阅读说明：本技术 酮还原酶多肽及多核苷酸 (Ketoreductase polypeptides and polynucleotides ) 是由 杰弗里·C·穆尔 杰克·梁 乔纳森·彭菲尔德 约瓦娜·纳佐尔 尼基·德拉斯 韦丝娜·米切尔 于 2018-04-13 设计创作，主要内容包括：本发明提供了与天然存在的野生型酮还原酶和亚磷酸脱氢酶相比具有改进的特性的工程化酮还原酶和亚磷酸脱氢酶、以及编码工程化酮还原酶和亚磷酸脱氢酶的多核苷酸、能够表达工程化酮还原酶和亚磷酸脱氢酶的宿主细胞、以及使用工程化酮还原酶和亚磷酸脱氢酶来合成用于抗病毒化合物诸如核苷抑制剂的合成的手性催化剂的方法。本发明还提供了使用工程化酶在一锅多酶系统中使手性醇去外消旋化的方法。(The present invention provides engineered ketoreductases and phosphite dehydrogenases having improved properties compared to naturally occurring wild-type ketoreductases and phosphite dehydrogenases, as well as polynucleotides encoding the engineered ketoreductases and phosphite dehydrogenases, host cells capable of expressing the engineered ketoreductases and phosphite dehydrogenases, and methods of using the engineered ketoreductases and phosphite dehydrogenases for the synthesis of chiral catalysts for the synthesis of antiviral compounds, such as nucleoside inhibitors. The invention also provides a method for deracemizing chiral alcohols in a one-pot multi-enzyme system using engineered enzymes.)