阅读说明：本技术 一种天麻保健酸奶及其制备方法 (Gastrodia elata health-care yoghourt and preparation method thereof ) 是由 邹娟 伍贤进 蒋忠权 陈三春 尹庆铁 田玉桥 曾申磊 于 2019-11-25 设计创作，主要内容包括：本发明公开了一种天麻保健酸奶,其原料组分由鲜牛奶、天麻多糖酶解液、糖、酸奶发酵剂组成,其中鲜牛奶、天麻多糖酶解液、糖、酸奶发酵剂的用量按质量配比为：1：0.05-0.1：0.05-0.1：0.02-0.05,本发明还公开了一种天麻保健酸奶的制备方法,该方法通过优化酶反应时间、pH值和酶用量,得到天麻多糖得率在5.03％以上的酶解液,进而增加可溶性多糖的含量,再配合糖和发酵酸奶,可获得组织细腻、凝块细小、均匀、无气泡并且口感佳、营养丰富的凝固型天麻酸奶。(The invention discloses a gastrodia elata health-care yoghourt, which comprises the following raw material components of fresh milk, gastrodia elata polysaccharide enzymatic hydrolysate, sugar and a yoghourt fermenting agent, wherein the fresh milk, the gastrodia elata polysaccharide enzymatic hydrolysate, the sugar and the yoghourt fermenting agent are used in the following mass ratio: 1: 0.05-0.1: 0.05-0.1: 0.02-0.05, and the invention also discloses a preparation method of the gastrodia elata health-care yoghourt, which comprises the steps of optimizing enzyme reaction time, pH value and enzyme dosage to obtain enzymatic hydrolysate with the gastrodia elata polysaccharide yield of more than 5.03%, further increasing the content of soluble polysaccharide, and then matching sugar and fermented yoghourt to obtain the set gastrodia elata yoghourt with fine and smooth tissue, fine and uniform coagulum, no bubbles, good taste and rich nutrition.)

1. The gastrodia elata health-care yoghourt is characterized by comprising the following raw material components of fresh milk, gastrodia elata polysaccharide enzymatic hydrolysate, sugar and a yoghourt fermenting agent, wherein the fresh milk, the gastrodia elata polysaccharide enzymatic hydrolysate, the sugar and the yoghourt fermenting agent are used in the following mass ratio: 1: 0.05-0.1: 0.05-0.1: 0.02-0.05.

2. The gastrodia elata health-care yoghourt as claimed in claim 1, wherein the fresh milk, the gastrodia elata polysaccharide enzymolysis liquid, the sugar and the yoghourt starter are used in the following amounts by mass: 1: 0.09: 0.08: 0.04.

3. the gastrodia elata health-care yoghourt as claimed in claim 1 or 2, wherein the preparation method of the gastrodia elata polysaccharide enzymolysis liquid comprises the following steps:

(1) pulping: soaking fresh rhizoma Gastrodiae in water for 10-20min, cleaning with clear water, steaming at 100-;

(2) enzymolysis: according to the following steps: 1-1.5, adding a citric acid-dipotassium hydrogen phosphate buffer solution with the pH value of 5.0-7.0 into the gastrodia elata homogenate obtained in the step (1), and adding a buffer solution which is mixed with the gastrodia elata homogenate in a mass ratio of 0.001-0.008: 1, carrying out enzymolysis for 2-5h at the temperature of 50-60 ℃ to obtain the gastrodia elata polysaccharide enzymolysis liquid.

4. The health yoghourt of claim 3, wherein in the step (2) of the preparation method of the polysaccharide enzymatic hydrolysate of Gastrodia elata, the pH value of the citric acid-dipotassium hydrogen phosphate buffer solution is 6.0.

5. The gastrodia elata health-care yoghourt as claimed in claim 3, wherein in the step (2) of the gastrodia elata polysaccharide enzymatic hydrolysate preparation method, the mass ratio of the hemicellulase to the gastrodia elata homogenate is 0.001: 1.

6. the gastrodia elata health-care yoghourt as claimed in claim 3, wherein in the step (2) of the gastrodia elata polysaccharide enzymolysis liquid preparation method, the enzymolysis temperature is 56 ℃.

7. The gastrodia elata health-care yoghourt as claimed in claim 3, wherein in the step (2) of the gastrodia elata polysaccharide enzymolysis liquid preparation method, the enzymolysis time is 4 hours.

8. The preparation method of the gastrodia elata health-care yoghourt according to any one of claims 1-7, characterized by comprising the following steps of:

step 1, dissolving: heating fresh milk in terms of 100g of fresh milk, adding the sugar in the mass ratio, and stirring until the sugar is completely dissolved;

step 2, blending: cooling the fresh milk treated in the step (1) to 38-44 ℃, inoculating the yoghourt leavening agent in the mass ratio into the fresh milk, simultaneously adding the gastrodia elata polysaccharide enzymatic hydrolysate in the mass ratio, and uniformly stirring;

step 3, fermentation: fermenting the fresh milk prepared in the step 1 at the temperature of 38-45 ℃ for 4-6h to obtain yoghourt;

step 4, refrigerating: and (4) refrigerating the yoghourt obtained in the step (3) at the temperature of 4-7 ℃ for 8-12h to obtain the gastrodia health-care yoghourt.

Technical Field

The invention belongs to the field of food processing, and particularly relates to gastrodia elata health-care yoghourt and a preparation method thereof.

Background

The gastrodia elata is a traditional rare Chinese medicinal material in China, has a history of more than two thousand years of medicine use, is a rootless heterotrophic orchidaceae plant, is mainly nourished by Armillaria mellea hypha invading into a epidermal layer after seeds germinate, and researches show that the main medicinal effect component in the gastrodia elata is gastrodia elata polysaccharide which has the effects of improving migraine, reducing blood pressure, preventing and treating senile dementia, resisting depression, improving sleep and the like, and can be widely applied to the fields of medical care and food.

The production process of the gastrodia elata polysaccharide is multiple, at present, a water extraction method, an alcohol extraction method, a microwave ultrasonic wave auxiliary method and the like are mostly adopted, and then the gastrodia elata polysaccharide is further separated and purified through a salt precipitation method, a gel chromatography method and the like to obtain the single-component polysaccharide. Although the existing extraction and separation method has the advantages of low price, no toxicity, safe operation and the like, the extraction time is too long, the yield is low, and the medicinal value of the gastrodia elata polysaccharide cannot be fully realized, so that how to improve the yield of the gastrodia elata polysaccharide and simplify the process becomes one of the key points of the current research.

The yogurt is a fermented food, contains digestible protein, amino acid, peptide and lactic acid, and has effects of improving lactose intolerance, enhancing immunity, etc., and has good flavor and health promotion effect, and is suitable for daily drinking. The research on the preparation of the gastrodia elata yoghourt by taking the gastrodia elata as the raw material is not complete at present.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide the gastrodia elata health-care yoghourt and the preparation method thereof, the enzymolysis liquid with the gastrodia elata polysaccharide yield of more than 5.03% is obtained by optimizing the enzyme reaction time, the pH value and the enzyme dosage, the content of soluble polysaccharide is further increased, and the solidified gastrodia elata yoghourt with fine and smooth tissue, fine and uniform coagulum, no bubbles and good taste can be obtained by matching sugar and fermented yoghourt.

In order to realize the purpose, the invention adopts the technical scheme that:

the gastrodia elata health-care yoghourt comprises the raw material components of fresh milk, gastrodia elata polysaccharide enzymatic hydrolysate, sugar and a yoghourt fermenting agent, wherein the fresh milk, the gastrodia elata polysaccharide enzymatic hydrolysate, the sugar and the yoghourt fermenting agent are used in the following mass ratio: 1: 0.05-0.1: 0.05-0.1: 0.02-0.05.

Preferably, the fresh milk, the gastrodia elata polysaccharide enzymolysis liquid, the sugar and the yoghourt starter are used in the following mass ratio: 1: 0.09: 0.08: 0.04.

preferably, the preparation method of the gastrodia elata polysaccharide enzymatic hydrolysate comprises the following steps:

(1) pulping: soaking fresh rhizoma Gastrodiae in water for 10-20min, cleaning with clear water, steaming at 100-;

(2) enzymolysis: according to the following steps: 1-1.5, adding a citric acid-dipotassium hydrogen phosphate buffer solution with the pH value of 5.0-7.0 into the gastrodia elata homogenate obtained in the step (1), and adding a buffer solution which is mixed with the gastrodia elata homogenate in a mass ratio of 0.001-0.008: 1, carrying out enzymolysis for 2-5h at the temperature of 50-60 ℃ to obtain the gastrodia elata polysaccharide enzymolysis liquid.

Preferably, in the step (2) of the method for preparing the gastrodia elata polysaccharide enzymatic hydrolysate, the pH value of the citric acid-dipotassium hydrogen phosphate buffer solution is 6.0.

Preferably, in the step (2) of the preparation method of the gastrodia elata polysaccharide enzymatic hydrolysate, the mass ratio of the hemicellulase to the gastrodia elata homogenate is 0.001: 1.

preferably, in the step (2) of the preparation method of the gastrodia elata polysaccharide enzymatic hydrolysate, the enzymolysis temperature is 56 ℃.

Preferably, in the step (2) of the preparation method of the gastrodia elata polysaccharide enzymatic hydrolysate, the enzymolysis time is 4 hours.

A preparation method of gastrodia elata health-care yoghourt comprises the following steps:

step 1, dissolving: heating fresh milk in terms of 100g of fresh milk, adding the sugar in the mass ratio, and stirring until the sugar is completely dissolved;

step 2, blending: cooling the fresh milk treated in the step (1) to 38-44 ℃, inoculating the yoghourt leavening agent in the mass ratio into the fresh milk, simultaneously adding the gastrodia elata polysaccharide enzymatic hydrolysate in the mass ratio, and uniformly stirring;

step 3, fermentation: fermenting the fresh milk prepared in the step 1 at the temperature of 38-45 ℃ for 4-6h to obtain yoghourt;

step 4, refrigerating: and (4) refrigerating the yoghourt obtained in the step (3) at the temperature of 4-7 ℃ for 8-12h to obtain the gastrodia health-care yoghourt.

The invention has the beneficial effects that: the raw material gastrodia elata polysaccharide enzymolysis liquid adopted by the invention is prepared by adopting an enzymolysis method, when the temperature, time, pH value and enzyme dosage of an enzyme reaction are controlled within the above ranges, the yield of the gastrodia elata polysaccharide reaches more than 5.03 percent, and the gastrodia elata polysaccharide serving as a medicinal component has the effects of improving migraine, reducing blood pressure, preventing and treating senile dementia, resisting depression, improving sleep and the like, and can be widely applied to the fields of medical care and food; the invention uses the rhizoma gastrodiae enzymatic hydrolysate as a raw material to prepare the set rhizoma gastrodiae yoghourt, adjusts the adding amount of the rhizoma gastrodiae yoghourt and the using amount of sugar and yoghourt leavening agent, and can prepare the set rhizoma gastrodiae yoghourt with fine and smooth tissue, fine and uniform coagulum, no bubbles, good taste and rich nutrition after process optimization; the yogurt combines the unique flavor of rhizoma Gastrodiae and the health promotion function of rhizoma Gastrodiae, and can satisfy various demands of consumers.

Detailed Description

For a better understanding of the present invention, embodiments of the present invention are described in detail below with reference to examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention.

The sugar used in this example was sucrose.