阅读说明：本技术 巴西苏木素增效多黏菌素类抗生素对大肠杆菌的抑菌效果应用 (Application of brazilin synergistic polymyxin antibiotics in bacteriostasis effect on escherichia coli ) 是由 郝智慧 任海燕 胡龙飞 刘旭东 王苹苹 王帅玉 于 2021-06-29 设计创作，主要内容包括：本发明涉及巴西苏木素增效多黏菌素类抗生素对鸡源大肠杆菌的抑菌效果的用途,本发明首次发现了巴西苏木素能够增效多黏菌素类抗生素对大肠杆菌的抑菌效果,使最低抑菌浓度明显降低。提供了新的联合用药的策略,同时巴西苏木素作为一种天然中药化合物,具有重要的药物研发和临床应用价值。(The invention relates to application of brazilein to synergism of polymyxin antibiotics to escherichia coli of chicken origin, and finds that brazilein can be used for synergism of the polymyxin antibiotics to escherichia coli for the first time, so that the minimum inhibitory concentration is obviously reduced. Provides a new drug combination strategy, and simultaneously brazilin is used as a natural traditional Chinese medicine compound, thereby having important drug research and development and clinical application values.)

1. A pharmaceutical combination comprising brazilin and polymyxin.

2. The pharmaceutical combination according to claim 1, wherein the polymyxin is selected from the group consisting of: polymyxin A, B, C, D, E, K, M and P.

3. The pharmaceutical combination according to claim 2, wherein: the polymyxin is selected from polymyxin B and polymyxin E.

4. The pharmaceutical combination according to claim 3, wherein: the polymyxin is polymyxin E.

5. The pharmaceutical combination according to claim 4, wherein: in the combination, the weight ratio of brazilein to polymyxin is 32-64: 1.

6. The pharmaceutical combination according to claim 1, characterized in that: the combination comprises brazilein and polymyxin which are mixed together to prepare a pharmaceutical composition, and the pharmaceutical composition is applied simultaneously when in use, or the brazilein and the polymyxin are respectively prepared into the pharmaceutical composition and are used jointly when in use.

7. The pharmaceutical combination according to claim 6, wherein: the pharmaceutical composition prepared by mixing the brazilein and the polymyxin can also comprise other components, and the brazilein and the polymyxin are respectively prepared into the pharmaceutical composition and are used in combination when in use, and can also comprise the combination of other components.

8. The pharmaceutical combination according to claim 7, wherein: the pharmaceutical composition is in the form of a pharmaceutical preparation and is selected from oral preparations and non-oral preparations, wherein the non-oral preparations comprise injections.

9. The application of brazilin is that the brazilin has the effect of increasing the resistance of polymyxin antibiotics to chicken escherichia coli.

10. The use according to claim 9, wherein the ratio of brazilin to polymyxin is 32-64:1 by weight, the two have a synergistic effect.

Technical Field

The invention belongs to the technical field of medicines, particularly relates to combined application of brazilein in synergistic polymyxin antibiotics against chicken-origin escherichia coli, and belongs to the field of animal bacterial disease prevention and treatment.

Background

Colibacillosis in chickens is an acute or chronic bacterial infectious disease of chickens caused by pathogenic escherichia coli.

The harm of chicken escherichia coli has a tendency of gradually expanding in recent years, and the number of dead chickens in a chicken farm caused by the escherichia coli is more than 50% of the number of dead chickens. To improve the survival rate and the laying rate of chicken flocks and improve the breeding level, a next-generation husband has to well control the colibacillosis of the chickens. The traditional Chinese medicine composition comprises various diseases such as colibacillary peritonitis, salpingitis, umbilicitis, synovitis, air sacculitis, granuloma, ophthalmia and the like, and is relatively harmful to the chicken industry. Polymyxin antibiotics (often referred to as polymyxin for short) are cationic polypeptides with broad-spectrum negative-bacteria resistance, mainly through the action of polycation rings in molecules and lipoid A on Lipopolysaccharide (LPS) of bacterial cell outer membrane, cations such as calcium, magnesium and the like in the outer membrane are replaced, the negatively charged outer membrane of negative bacteria is damaged, the stability of the cell membrane is reduced, the permeability of the cell outer membrane is increased, and important substances in cells flow out to generate bactericidal action. Polymyxins can be classified into 8 kinds of polymyxins A, B, C, D, E, K, M and P according to their chemical structures, wherein only Polymyxin B (Polymyxin B) and Polymyxin E (Polymyxin E, also known as Colistin) have low toxicity and are widely used in clinic, and the rest of polymyxins cannot be used in clinic due to excessive toxicity. Polymyxin B and colistin were used in the 1960 s for the treatment of severe pseudomonas aeruginosa or other gram negative bacilli infections, and since then these two drugs have been gradually replaced due to the continued development of new low-toxicity, potent antibiotics. With the wide clinical application of antibiotics and the continuous serious drug resistance, the curative effect of common antibacterial drugs (antibiotics and synthetic antibacterial drugs) is greatly reduced. Polymyxin is praised at home and abroad as the last line of defense for clinical treatment of multiple drug-resistant gram-negative bacterial infection due to low drug resistance. However, as the clinical, animal and agricultural use of polymyxin increases year by year and other antibiotic abuse conditions occur, the therapeutic effect on gram-negative bacteria is relatively reduced, and various polymyxin-resistant gram-negative bacteria appear. Therefore, under the condition of not increasing the dosage of polymyxin and even reducing the dosage, the synergist is utilized to effectively treat bacterial infection, particularly polymyxin-resistant bacterial infection, and the application value of the synergist in clinical application is important.

Brazilin is one of the main active ingredients extracted from heartwood of sappan wood.

Brazilin has activity against various bacteria, such as mammary gland inflammation caused by Staphylococcus aureus. In vitro experiments show that the combination of the hematoxylin and the aminoglycoside has synergistic effect on the bactericidal activity of methicillin-resistant staphylococcus aureus. Studies find that the hematoxylin can also inhibit the growth of staphylococcus aureus and staphylococcus epidermidis, and some components of the hematoxylin also have an inhibitory effect on streptococcus, and the action mechanism is realized by inhibiting the formation of a biological membrane.

The dosage of polymyxin: the recommended dose of the polymyxin sulfate oral liquid is 0.2mL/kg per time and 2 times per day for 2 days. [1] And the clinical test of the synergistic polymyxin sulfate oral liquid for treating yellow and white scour of piglets [ J ]. Guangxi agricultural science, 2009,40 (02): 210-212.

Dosage of brazilein: the hematoxylin is poured into hematoxylin granules dissolved in 2ml of distilled water every day according to the weight crude drug amount of 810mg/kg equivalent human dose. [2] Liuting, sappan wood and brazilin regulate the effect of autophagy on high-sugar-induced vascular endothelial cell injury [ D ]. department of medicine, university of california, 2017.

Disclosure of Invention

The brazilein has good bacteriostatic effect, but the research on chicken-derived drug-resistant escherichia coli is less.

Brazilein and polymyxin are used independently to treat chicken-derived escherichia coli, and the effect is poor.

The invention discovers that the brasilein can obviously enhance the antibacterial activity of the polymyxin and reverse the drug resistance of chicken-origin drug-resistant escherichia coli to the polymyxin when the novel application of the synergistic polymyxin antibiotics to chicken-origin escherichia coli is combined with the polymyxin. Wherein the weight ratio of the brazilein to the polymyxin is 32-64:1, so that the effect is obvious.

Therefore, the invention provides an application of brazilin, which is characterized in that the brazilin has a synergistic effect in the polymyxin antibiotics against the chicken escherichia coli. Wherein the weight ratio of brazilein to polymyxin is 32-64: 1.

The brazilin is applied in that the brazilin has the function of increasing the resistance of polymyxin antibiotics to chicken escherichia coli. Wherein the weight ratio of brazilein and polymyxin is 32-64:1, the two have synergistic effect.

The invention provides a combination comprising brazilein and polymyxin.

The polymyxins in the combination of the invention are polymyxin B and polymyxin E.

In the combination of the invention, the weight ratio of brazilein to polymyxin is 32-64: 1.

The combination comprises brazilein and polymyxin which are mixed together to prepare a pharmaceutical composition, and the pharmaceutical composition is applied simultaneously when in use, or the brazilein and the polymyxin are respectively prepared into the pharmaceutical composition and are used jointly when in use.

The pharmaceutical composition prepared by mixing brazilein and polymyxin can also comprise other components.

The brazilein and polymyxin are respectively prepared into pharmaceutical compositions, and are used in combination, and other components can also be used in combination.

The pharmaceutical composition is in the form of a pharmaceutical preparation and is selected from oral preparations and non-oral preparations, wherein the non-oral preparations comprise injections.

Experiments show that the compound medicine prepared from brazilein and polymyxin according to the weight ratio of 32-64:1 has the best treatment effect and the effect of eliminating the drug resistance of chicken-origin escherichia coli, and the composition can effectively inhibit the drug resistance of chicken-origin escherichia coli by eliminating the biofilm of the escherichia coli. In order to make the objects, technical solutions and advantages of the present invention clearer, the following further illustrates the beneficial effects of the present invention through specific experimental data. The beneficial effects of the present invention are further illustrated by experimental data below. The standard strain ATCC 25922.

Experiment 1: effect observation of brazilin and polymyxin on drug resistance of chicken-origin escherichia coli

(1) Experimental article

(ii) an experimental Strain

The strain used in the invention is chicken-origin drug-resistant escherichia coli E.coli-4 separated in a laboratory and purchased from China center for industrial microorganism culture collection.

② the test medicine brazilin CAS 474-07-7 Chengduieisi Biotechnology limited company, the content is: is more than 98 percent. Polymyxin B19000U/mg Shanghai Bioengineering, Inc.

Polymyxin E19000U/mg Shanghai Bioengineering, Inc.

③ dissolving the brazilein prepared from the medicines with sterile water to prepare a mother solution of 5mg/mL, and storing the mother solution in a refrigerator at 4 ℃. Polymyxin: dissolving the drug in small amount of sterile water (100 μ L), diluting with water to obtain drug stock solution with final concentration of 5120 μ g/mL, filtering with 0.22 μm filter membrane, packaging into sterile 2mL centrifuge tube, and storing at-80 deg.C

Fourthly, culture medium

LB broth culture medium: qingdao Haibo Biotechnology Ltd, configured according to the instructions.

MH broth medium: qingdao Haibo Biotechnology, Inc. was configured according to the instructions.

Fifthly, the electric pipette of the instrument is AUTOCLAVABLE: eppendorf, Germany;

electronic balance (ME203) mettler-toledo instruments (shanghai) ltd;

vertical automatic pressure steam sterilizer (GI80 DS): pico (xiamen) instruments ltd;

portable bacteria turbidimeter: shanghai Xinrui instruments & meters Limited;

double single-side vertical superclean bench: suzhou clarification plant Co Ltd

Preparation of bacterial liquid

Picking single colony on streaked Macconkey agar culture medium, inoculating to 5ml LB broth culture medium, shake culturing at 37 deg.C in a shaker at 180rpm, placing the bacteria at late growth phase, and adding the bacteria liquid into MH broth culture medium to make the concentration of the bacteria liquid reach 10⁵CFU/mL. Adding polymyxin to the bacterial suspension at a dose to a desired concentration for use.

(2) MIC determination and determination

The Minimum Inhibitory Concentrations (MICs) of colistin and brazilin against the test strains were measured by the twofold minimal broth dilution method, with reference to the procedures specified in CLSI. Placing required 96-well plate, disposable culture dish, sterile culture medium, sterile water and the like in an ultra-clean workbench, performing ultraviolet sterilization for 30min in advance, preparing polymyxin B and polymyxin E mother liquor (prepared at present) after sterilization, and diluting the polymyxin mother liquor with sterile water to prepare the polymyxin mother liquor (with the concentration range of 512 mu g/mL to 0.06 mu g/mL).

Secondly, the sterile MH liquid culture medium is subpackaged in each hole of a 96-hole plate, 50 mu L of each hole is filled with 50 mu L of prepared antibiotics with different concentrations, 2-time dilution is carried out (from the concentration range of 256-0.03 mu g/mL), finally, each hole contains polymyxin with different concentrations, the volume is 100 mu L, and 100 mu L of prepared bacteria liquid is supplemented in each hole (the final concentration of the bacteria liquid is equal to 5 multiplied by 10)⁵CFU/mL), the added 96-well plate is mixed evenly by micro-shaking, and then the mixture is placed in a constant temperature incubator (37 ℃) for static culture for 18 to 24 hours.

Observation of the broth dilution method to test the minimum inhibitory concentration of polymyxin on the used strains: the minimum inhibitory concentration for the modified bacteria is determined by the concentration of polymyxin 2 times corresponding to the turbidity visible to the naked eye in each row of the wells, if the phenomenon of hole jumping occurs, the problem of improper operation or pollution is indicated, the result is invalid, and all the tests are repeated for 3 times. The results are shown in tables 1 and 2.

By observing the transparency of the cultures in the 96-well plates, it can be seen from Table 1 that the MIC values of polymyxin for E.coli R-4 were all 8. mu.g/mL and for the standard strain were 0.25. mu.g/mL. The MIC value of brazilin to E.coli R-4 was 256. mu.g/mL, and the MIC to the standard strain was 512. mu.g/mL

TABLE 1 minimum inhibitory concentration test results for polymyxin against test strains

Table 2 results of minimum inhibitory concentration test of brazilin on test strains

Strain name	The source of the strain	Brazilian hematoxylin MIC/mu g/mL
			E.coli R-4	Shandong Qingdao poultry excrement	256
ATCC25922	China center for preservation of industrial microbial strains	512

Experiment 2: chessboard method for measuring brazilein and polymyxin FIC

(1) Test drugs: polymyxin E and brazilin

(2) The experimental method comprises the following steps:

(ii) picking single colony from MH solid medium and placing it in a culture medium containing MH 2. mu.g/mLAdding into liquid culture medium, culturing in constant temperature incubator until logarithmic phase, diluting with sterile culture medium to bacterial liquid amount (1 × 10)⁶/mL CFU/mL) as a stock solution.

Thirdly, taking out a sterile 96-well plate, adding 100 mu L A medicines into the 1 st row, adding 50 mu L MH broth into the 2 nd to 10 th rows, adding 100 mu L MH broth and 100 mu L bacterial liquid into the 11 th row, adding 200 mu L broth into the 12 th row, taking out 50 mu L from the 1 st row, diluting the 50 mu L in the 2-10 th rows from left to right times, diluting the traditional Chinese medicine monomers in each left row by 2 times, discarding 50 mu L from the 10 th hole, adding 1-6 medicines with gradually-reduced concentration gradient in an external centrifuge tube, and adding 100 mu L of different bacterial liquids into each hole until the total volume of each hole is 200 mu L. The row 7 is 100 mu L A single drug component plus 100 mu L bacterial liquid. The 8 th row is 100 mu L B single drug component plus 100 mu L bacterial liquid. Since brazilin is easily oxidized, it was rapidly dissolved in a 96-well plate in the dark, and compared with the normal assay group, the effect was different, and the test for each drug was repeated three times.

③ chessboard method result interpretation standard

The graded bacteriostasis concentration index FIC value can be used as the basis for evaluating the results and judging the curative effect of various combined medicament sensitive bacteria tests, and the calculation formula is briefly described as follows:

the FIC index is used to determine the criteria for synergistic effects of two antibacterial agents (FICI).

Wherein FICI is less than or equal to 0.5, and the synergistic effect is obtained; FICI of more than or equal to 0.5 and less than or equal to 1 is an additive effect; the FICI is more than 1 and less than or equal to 2 as an irrelevant effect; FICI > 2 is antagonistic.

Results of experiment 2:

as shown in table 3, the corresponding 96-well plate bacterial-free growth pore was selected for statistical drug dosage, and the FIC mean value of the combined action of alkyl gallate and streptomycin was obtained after repeated experiments according to the FIC calculation formula, as shown in table 3, when brazilin and polymyxin were used in combination, the FIC value of the test strain e.coli R-4 was 0.31, and the FIC was less than 0.5, which had a synergistic effect. The FIC value of the standard strain ATCC25922 is 0.26 and the FIC is less than 0.5. FIC of strain e.coli R-4 is 64 ÷ 256+1 ÷ 8 ═ 0.375

The standard strain ATCC25922 has FIC 4 ÷ 512+0.06 ÷ 0.25 ÷ 0.248

TABLE 3 minimum inhibitory concentrations of brazilin in combination with polymyxin against different isolates

Experiment 3: time sterilization curve experiment

(ii) overnight-cultured E.coli R-4 and ATCC25922 were adjusted to 5 xl 0⁷CFUs/mL, ready for use. Taking 3 groups of sterile test tubes (1 group: positive control group, 2 groups: single antibiotic group, 3 groups: antibiotic and drug combination group), marking each group as 0, 1, 3, 5, 8h and 12h, adding L mL of high-pressure sterilized LB liquid culture medium into all test tubes, adding 10 mu L of adjusted bacterial liquid into each tube, and leading the final concentration of the bacterial liquid in each test tube to be 5 multiplied by 10⁵CFUs/mL。

And secondly, carrying out different treatments on different groups, immediately mixing the treated groups, taking out 10 mu L of the positive control group bacterial liquid, coating the plate, and diluting the plate to a proper concentration by 10 times through normal saline to obtain the colony count of 0 h. And then, respectively taking 10 mu L of bacterial liquid at each time point on an LB agar plate, uniformly shaking the autoclaved and dried glass beads on a flat plate until the glass beads are air-dried, taking out the glass beads until the glass beads are air-dried, placing the flat plate coated at each time period in a constant-temperature incubator at 37 ℃, culturing for 22-24 h, calculating the number of grown bacteria, arranging 3 parallel controls in each group, repeating the steps for three times, and drawing a time-sterilization curve after obtaining a result.

③ judging and reading the result:

allowable difference for 3 replicates at the same dilution:

experimental results:

according to the sterilization curve result, the strain R-4 shows a logarithmic growth trend within 1-7 h in the blank control group, and reaches a growth stationary phase within 7-12 h. Compared with a blank control group, after the effect of 64 mu g/mL of brazilein and 1 mu g/mL of polymyxin are independently added, the growth trend of the bacteria in 1-7 h is slightly lower than that of the blank control group, but the bacteria grow repeatedly in the period of 7h, the number of colonies is counted by dropping plates, the number of the colonies added with brazilein is slightly reduced by about 0.3 multiplied by log CFU/mL compared with that of the blank group in 1-12 h, the growth of the strain is temporarily inhibited in 1-6 h after the single polymyxin is added, the strain grows again after 6h, and the result is basically consistent with that of the blank control group at 12 h.

In the drug combination group, after the combination of 64 mug/mL brasilein and 1 mug/mL polymyxin, the growth of bacteria amount is inhibited and slightly reduced, compared with the blank control group, the bacteria amount is reduced by about 5.3 XlogCFU/mL at 12h, although the bacteria are not completely killed, the bacteria growth of the combination group is always inhibited.

Experiment 4 the Effect of Brazilian hematoxylin in combination with polymyxin on Chicken-derived Escherichia coli biofilm

(1) Experimental strain test strain e.coli R-4, standard strain ATCC25922

(2) Medicine preparation: brazilin and polymyxin

(3) The experimental method comprises the following steps: scanning electron microscope for determining influence on thallus structure of escherichia coli

Selecting monoclonals of a test strain E.coli R-4 and a standard quality control strain ATCC25922 on an LB plate, placing the monoclonals in 5mL of LB liquid medium for overnight culture at constant temperature, adding 5mL of LB liquid medium according to drug grouping, adjusting the final drug concentration to be (1 group: polymyxin single use group 1 mu g/mL, 2 group: brazilein single use group 64 mu g/mL, 3 group: combination drug group: 64 mu g/mL brazilein +1 mu g/mL polymyxin 4 group: blank control group), and performing shake culture on a shaker until the growth log phase of the bacteria.

Secondly, 1mL of the bacterial liquid is taken out, centrifuged by 1 XPBS buffer solution 4600r/min for 5min, absorbed by a pipette and slowly and repeatedly rinsed for three times, after rinsing, 10 mu L of the liquid is placed on a glass slide, naturally dried in an ultra-clean bench, added with 5% glutaraldehyde for fixation, reacted for 4h at room temperature, and placed in a refrigerator at 4 ℃ for standing for 12h for later use;

③ dehydrating by using absolute ethyl alcohol with different gradients: dehydrating with 30% ethanol for 15min, dehydrating with 50% ethanol for 15min, dehydrating with 90% ethanol for 15min, and dehydrating with 100% ethanol for 20 min. And (5) absorbing the excessive water by using filter paper, and naturally drying.

And fourthly, observing the treated sample by using a scanning electron microscope.

(4) The experimental results are as follows:

it can be observed from the scanning electron microscope picture that, compared with the blank control group (figure a), the test strain e.coli R-4 has almost no change or influence of the drug on the form of escherichia coli when 1 μ g/mL polymyxin is used alone (figure B), the outer wall of the test strain has slight structural change and damage in the presence of 64 μ g/mL brazilian hematoxylin (figure C), when 64 μ g/mL brazilian hematoxylin is used in combination with 1 μ g/mL polymyxin (figure D), the outer wall of the cell of the bacterium starts to disintegrate and break, the content is leaked and the shape is irregular, which indicates that the effect of the drug on the bacterial structure is not great when the drug is used alone, and the drug combination inhibits the generation of the drug resistance of the bacterium by changing the bacterial structure after the drug combination.

Drawings

FIG. 1 is a graph showing the bactericidal activity of strain R-4

FIG. 2 is a diagram showing the morphology of bacterial cells under scanning electron microscope of strain E

Detailed Description

The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto.

Example 1

Prescription and packaging of polymyxin dosage forms:

packaging polymyxin 0.1g in powder form

Brazilin drugs:

packaging 3.2-6.4 g brazilein in the form of powder

And (3) combined packaging:

respectively packaging polymyxin and brazilein into respective medicine, then packaging the two medicines into the same large packaging box, and taking the two boxes of powder with water when in use.

Example 2

Polymyxin drugs and packaging:

the polymyxin tablet is prepared into 0.5g tablet, and the effective dose of polymyxin tablet is 0.45g

Brazilian hematoxylin medicine and package

Brazilein was packed in powder form at a weight ratio of 1: 32 in 14.4g in powder form. And (3) combined packaging:

respectively packaging polymyxin tablet and brazilin powder into respective medicine, and then packaging the two medicines together into a large packaging box, wherein the polymyxin tablet is orally taken and brazilin is taken with water when in use.

Example 3

Polymyxin drugs and packaging:

the polymyxin tablet is prepared into 0.5g tablet, and the effective dose of polymyxin tablet is 0.45g

Brazilian hematoxylin medicine and package

Brazilein was packed in powder form at a weight ratio of 1: 64 in 28.8g in powder form. And (3) combined packaging:

respectively packaging polymyxin tablet and brazilin powder into respective medicine, and then packaging the two medicines together into a large packaging box, wherein the polymyxin tablet is orally taken and brazilin is taken with water when in use.