阅读说明：本技术 一种从绿茶中提取茶氨酸的制备方法 (preparation method for extracting theanine from green tea ) 是由 杨成东 于 2018-05-29 设计创作，主要内容包括：本发明公开了一种从绿茶中提取茶氨酸的制备方法,其特征在于以下步骤：1)取干绿茶原料于50℃烘干粉碎,过40目筛,收集粗粉备用；2)酶解：称取一定量的粗粉,加入料液比为1：3~5,60%的乙醇浸泡,并加入粗粉重量0.1%~0.3%的生物酶在pH值5.6,温度为50℃的条件下浸泡酶解2h；3)将上述酶解液放入温度为70℃的恒温水浴锅内浸提90-120min,浓缩；4)将所得浓缩液上大孔吸附树脂柱,先用2~5倍柱体积的蒸馏水洗脱,再用40~90%的乙醇洗脱,收集乙醇洗脱液；5)回收试剂,真空干燥即得茶氨酸。本发明制备的茶氨酸,产品纯度高,品质好。(The invention discloses a preparation method for extracting theanine from green tea, which is characterized by comprising the following steps: 1) drying and crushing a dried green tea raw material at 50 ℃, sieving the dried green tea raw material by a 40-mesh sieve, and collecting coarse powder for later use; 2) enzymolysis: weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3-5, soaking in 60% ethanol, adding 0.1-0.3% of biological enzyme by weight of the coarse powder, and soaking and performing enzymolysis for 2 hours at the pH value of 5.6 and the temperature of 50 ℃; 3) leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90-120min, and concentrating; 4) passing the obtained concentrated solution through a macroporous adsorption resin column, eluting with 2-5 times of column volume of distilled water, eluting with 40-90% ethanol, and collecting ethanol eluate; 5) recovering the reagent, and vacuum drying to obtain theanine. The theanine prepared by the invention has high purity and good quality.)

1. A preparation method for extracting theanine from green tea is characterized by comprising the following steps:

1) Drying and crushing green tea raw materials at 50 ℃, sieving the green tea raw materials by a 40-mesh sieve, and collecting coarse powder for later use;

2) Enzymolysis: weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3-5, soaking in 60% ethanol, adding 0.1-0.3% of bio-enzyme by weight of the coarse powder, and soaking and performing enzymolysis for 1-3 hours under the conditions that the pH value is 3.5-7.5 and the temperature is 50 ℃;

3) Leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90-120min, and concentrating;

4) passing the obtained concentrated solution through a macroporous adsorption resin column, eluting with 2-5 times of column volume of distilled water, eluting with 40-90% ethanol, and collecting ethanol eluate;

5) recovering the reagent, and vacuum drying to obtain theanine.

2. the method of claim 1, wherein the bio-enzyme of step 2) is a mixture of pectinase, cellulase and laccase.

3. The method of claim 1, wherein the macroporous adsorbent resin in step 5) is one of JAD-1000, ZJX, and HD-8.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of theanine.

Background

green tea is known as "national drink" in China. The tea has the pharmacological effects of refreshing, clearing away heart-fire, relieving summer-heat, promoting digestion, eliminating phlegm, reducing weight, clearing away heart-fire, relieving restlessness, removing toxic substances, relieving hangover, promoting salivation, quenching thirst, lowering fire, improving eyesight, relieving dysentery, eliminating dampness, etc., and also has certain pharmacological effects on modern diseases, such as radiation disease, cardiovascular and cerebrovascular diseases, cancer, etc. Modern science has proved that the tea contains biochemical components closely related to human health, the main components of the tea with pharmacological action are tea polyphenol, caffeine, lipopolysaccharide, theanine and the like, and the tea has multiple efficacies of anti-aging, anti-cancer, anti-virus, beautifying and protecting skin, refreshing brain, inducing diuresis and relieving fatigue, protecting teeth and improving eyesight, reducing blood fat and the like.

Theanine is a unique free amino acid in tea, is similar to active substances in brain, namely glutamine and glutamic acid in chemical structure, is a main component for promoting the secretion of saliva or body fluid and moistening sweet in tea, and accounts for 1-2% of the weight of dry tea. Theanine can obviously promote dopamine release in brain center and improve physiological activity of dopamine in brain. Dopamine is a precursor of adrenaline and adrenaline, is an important substance for conveying the excitation degree of brain nerve cells, and the release of dopamine can greatly influence the emotion, the learning ability, the memory and the like of a human body. In addition, theanine also has the effects of lowering blood pressure, assisting in inhibiting tumor, and influencing absorption and metabolism.

disclosure of Invention

the invention aims to provide a method for extracting theanine, and the theanine has wide medicinal value.

the invention is completed by the following technical scheme, and the specific content comprises the following steps: extraction method of theanine

1) Drying and crushing green tea raw materials at 50 ℃, sieving the green tea raw materials by a 40-mesh sieve, and collecting coarse powder for later use;

2) Enzymolysis: weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3-5, soaking in 60% ethanol, adding 0.1-0.3% of bio-enzyme by weight of the coarse powder, and soaking and performing enzymolysis for 1-3 hours under the conditions that the pH value is 3.5-7.5 and the temperature is 50 ℃;

3) Leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90-120min, and concentrating;

4) Passing the obtained concentrated solution through a macroporous adsorption resin column, eluting by using distilled water with the volume of 3 times of the column volume, eluting by using 40-90% ethanol, and collecting ethanol eluate;

5) recovering the reagent, and vacuum drying to obtain theanine.

The biological enzyme in the step 2) is a mixture of 0.543 percent of pectinase, 0.657 percent of cellulase and 0.600 percent of laccase.

The macroporous adsorption resin in the step 5) is one of JAD-1000, ZJX and HD-8 types.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows: