阅读说明：本技术 一种丁香精油皮克林乳液涂膜保鲜剂及其制备方法和应用 (Clove essential oil pickering emulsion coating preservative and preparation method and application thereof ) 是由 徐宝才 花璐 邓洁莹 王兆明 周辉 于 2021-09-02 设计创作，主要内容包括：本发明属于食品领域,具体涉及一种丁香精油皮克林乳液涂膜保鲜剂及其制备方法和应用。该制备方法,包括以下步骤,用乙醇和玉米醇溶蛋白粉末配置20mg/ml的玉米醇溶蛋白溶液,超纯水和酪蛋白酸钠配置0.4-6mg/ml的酪蛋白酸钠溶液,磁力搅拌过夜；将的20mg/ml玉米醇溶蛋白溶液逐滴滴入的0.4-6mg/ml的酪蛋白酸钠溶液中,在500rpm磁力搅拌30min；再使用旋转蒸发仪在40℃蒸发掉溶液中的乙醇,蒸发掉的乙醇用超纯水替代；然后加入丁香精油,高速均质3min,得到丁香精油皮克林乳液；1％v/v乙酸和壳聚糖配置1.5％-2％w/v壳聚糖溶液,在壳聚糖溶液中加入丁香精油皮克林乳液,高速均质3min,超声30min消泡,得到丁香精油皮克林乳液涂膜保鲜剂。(The invention belongs to the field of foods, and particularly relates to a clove essential oil pickering emulsion coating preservative as well as a preparation method and application thereof. The preparation method comprises preparing 20mg/ml zein solution from ethanol and zein powder, preparing 0.4-6mg/ml sodium caseinate solution from ultrapure water and sodium caseinate, and magnetically stirring overnight; dropwise adding the 20mg/ml zein solution into 0.4-6mg/ml sodium caseinate solution, and magnetically stirring at 500rpm for 30 min; evaporating the ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water; then adding clove essential oil, and homogenizing at high speed for 3min to obtain clove essential oil pickering emulsion; preparing 1.5% -2% w/v chitosan solution from 1% v/v acetic acid and chitosan, adding clove essential oil pickering emulsion into the chitosan solution, homogenizing at high speed for 3min, and defoaming by ultrasonic treatment for 30min to obtain the clove essential oil pickering emulsion coating preservative.)

1. A preparation method of clove essential oil pickering emulsion coating preservative is characterized by comprising the following steps,

step S1, preparing a zein solution of 20mg/ml by using ethanol and zein powder, preparing a sodium caseinate solution of 0.4-6mg/ml by using ultrapure water and sodium caseinate, and magnetically stirring for 6-12 hours;

s2, dropwise adding the 20mg/ml zein solution into 0.4-6mg/ml sodium caseinate solution, and magnetically stirring at 500rpm for 30 min;

step S3, evaporating ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water to ensure that the volume of the solution is not changed;

step S4, adding clove essential oil, and homogenizing at high speed for 3min to obtain clove essential oil pickering emulsion;

s5, preparing 1.5% -2% w/v chitosan solution from 1% v/v acetic acid and chitosan, adding 20% w/w glycerol into the chitosan solution, then adding the clove essential oil pickering emulsion into the chitosan solution, homogenizing at a high speed for 3min, and defoaming by ultrasonic wave for 30min to obtain the clove essential oil pickering emulsion coating preservative.

2. The preparation method of the clove essential oil pickering emulsion coating preservative as claimed in claim 1, wherein the concentration of the ethanol is 70% -80%.

3. The preparation method of the clove essential oil pickering emulsion coating preservative according to claim 1, wherein the ratio of the zein solution to the sodium caseinate solution in the step S2 is 1: 10.

4. The preparation method of the clove essential oil pickering emulsion coating preservative as claimed in claim 1, wherein the concentration of clove essential oil in the step S4 is 0.5% -2% v/v.

5. The method for preparing the clove essential oil pickering emulsion coating preservative according to claim 1, wherein the clove essential oil is added into the chitosan solution in the step S5, so that the concentration of the clove essential oil in the total solution is 0.2-0.6% v/v.

6. The method for preparing the clove essential oil pickering emulsion coating preservative as claimed in claim 1, wherein the high-speed homogenizing speed in the steps S4 and S5 is 15000-20000 rpm.

7. The clove essential oil pickering emulsion coating preservative prepared by the method of any one of claims 1 to 6.

8. The use of the clove essential oil pickering emulsion coating preservative of claim 7 as a food preservative.

9. The method for preserving the roasted chicken by adopting the clove essential oil pickering emulsion coating preservative as claimed in claim 7, is characterized in that: comprises the following steps of (a) carrying out,

step S1, soaking the fresh roast chicken in 0-4 ℃ clove essential oil Pickering emulsion coating preservative for 20-60S;

and step S2, taking out the fresh roast chicken from a bath for soaking the pick-up oil Pickering emulsion coating preservative, draining in a refrigerating chamber at 4 ℃, and putting into a fresh-keeping bag for refrigerating at 4 ℃.

Technical Field

The invention belongs to the field of foods, and particularly relates to a clove essential oil pickering emulsion coating preservative as well as a preparation method and application thereof.

Background

The plant essential oil is a hydrophobic compound with aromatic flavor generated by plants, and has safety, broad-spectrum antibacterial property and antioxidant activity. Hitherto, a plurality of plant essential oils are used as food preservatives to replace synthetic preservatives for food preservation, and clove essential oil is a natural plant essential oil with great potential for food preservation. The clove essential oil is extracted from the bud of clove trunk, and has biological activity and antibacterial activity due to the existence of high-concentration eugenol and other phenolic compounds, and can denature cell protein and perform chemical reaction with cell membrane phospholipid so as to change the permeability of the cell membrane phospholipid. The clove essential oil can kill some important food-borne pathogenic bacteria, including staphylococcus aureus, escherichia coli, listeria monocytogenes and salmonella typhi, and has anti-free radical and metal chelating activities. In addition, the clove essential oil has strong inhibition effect on other gram-positive bacteria, gram-negative bacteria and fungi. At present, the clove essential oil is widely applied to the field of food preservation and fresh keeping. Researches have shown that the clove essential oil can well delay the putrefaction degree of ham sausages by directly applying the clove essential oil to the ham sausages through a soaking method and inoculating staphylococcus aureus ATCC and Escherichia coli O157: H7; the clove essential oil, the mustard essential oil and the cinnamon essential oil are compounded, so that the synergistic effect is found to be achieved on the fresh-keeping effect of the fresh conditioned chicken, and the shelf life of the fresh conditioned chicken is prolonged to 21 days.

Although the clove essential oil can play the roles of antibiosis and antioxidation when being directly applied to food, the volatility of the clove essential oil can reduce the utilization rate of the clove essential oil, and the clove essential oil with high concentration causes the food to have strong special flavor of the clove essential oil. On the contrary, the essential oil with low concentration does not achieve the ideal sterilization effect. In addition, the bacteriostatic and antioxidant activities of the clove essential oil decrease faster with time during the food refrigeration. Therefore, the volatilization of the clove essential oil is inhibited by adopting an encapsulation technology, and the water solubility is improved, so that the utilization rate and the antibacterial property of the essential oil in the aspects of food preservation and fresh keeping are improved. The oil-in-water type nano emulsion has high stability, stronger physical and chemical properties, better controlled release performance and good bioactivity. To date, the common emulsifiers have been surfactants such as tween 20 and tween 80, and researchers have begun investigating natural compounds as solid particulate stabilizing actives. Different from the surfactant, a compact interface layer can be formed after the nano particles are adsorbed at an oil-water phase interface, so that the accumulation of liquid drops is effectively prevented. Meanwhile, the preparation method has better stability, superior embedding rate, lower electrolysis rate and better protection of active small molecules to a certain extent. Proteins and polysaccharides are often used as emulsifiers and stabilizers due to their thickening and gelling properties. The antibacterial and antioxidant active micromolecules are emulsified and filmed, and then are covered on the surface of the food in a wrapping, spraying, soaking and other modes to obstruct the permeation of oxygen, reduce the volatilization of flavor substances and the loss of moisture, increase the luster of the surface of the food, prevent the migration and diffusion of components in the food, reduce the oxidation of fat and reduce the growth of microorganisms, thereby delaying the putrefaction and deterioration of the food and prolonging the shelf life of the food.

Disclosure of Invention

Therefore, the clove essential oil pickering emulsion coating preservative, and the preparation method and the application thereof are needed to be provided. The invention adopts an anti-solvent method to prepare the zein/sodium caseinate nanoparticle embedded clove essential oil, and researches the influence of pickering emulsion containing different contents of clove essential oil on the mechanical property, water resistance, microstructure, antibacterial property and release rate of the chitosan membrane.

In order to achieve the purpose, the invention adopts the following technical scheme:

a preparation method of clove essential oil pickering emulsion coating preservative comprises the following steps,

step S1, preparing a zein solution of 20mg/ml by using ethanol and zein powder, preparing a sodium caseinate solution of 0.4-6mg/ml by using ultrapure water and sodium caseinate, and magnetically stirring for 6-12 hours;

s2, dropwise adding the 20mg/ml zein solution into 0.4-6mg/ml sodium caseinate solution, and magnetically stirring at 500rpm for 30 min;

step S3, evaporating ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water to ensure that the volume of the solution is not changed;

step S4, adding clove essential oil, and homogenizing at high speed for 3min to obtain clove essential oil pickering emulsion;

s5, preparing 1.5% -2% w/v chitosan solution from 1% v/v acetic acid and chitosan, adding 20% w/w glycerol into the chitosan solution, then adding the clove essential oil pickering emulsion into the chitosan solution, homogenizing at a high speed for 3min, and defoaming by ultrasonic wave for 30min to obtain the clove essential oil pickering emulsion coating preservative.

The technical scheme is further optimized, and the concentration of the ethanol is 70-80%.

According to the further optimization of the technical scheme, the ratio of the zein solution to the sodium caseinate solution in the step S2 is 1: 10.

According to the further optimization of the technical scheme, the concentration of the clove essential oil in the step S4 is 0.5% -2% v/v.

According to the further optimization of the technical scheme, after the clove essential oil pickering emulsion is added into the chitosan solution in the step S5, the concentration of the clove essential oil in the total solution is 0.2% -0.6% v/v.

In the further optimization of the technical scheme, the speed of high-speed homogenization in the steps S4 and S5 is 15000-20000 rpm.

The clove essential oil Pickering emulsion coating preservative prepared by the method of any one technical scheme is provided.

The clove essential oil pickering emulsion coating preservative is applied as a food preservative. Is applied to fruits, vegetables and meat products.

The method for keeping the freshness of the roast chicken by the clove essential oil Pickering emulsion coating preservative comprises the following steps,

step S1, soaking the fresh roast chicken in 0-4 ℃ clove essential oil Pickering emulsion coating preservative for 20-60S;

and step S2, taking out the fresh roast chicken from a bath for soaking the pick-up oil Pickering emulsion coating preservative, draining in a refrigerating chamber at 4 ℃, and putting into a fresh-keeping bag for refrigerating at 4 ℃.

Different from the prior art, the technical scheme has the following beneficial effects:

1. the zein-sodium caseinate nano particles are used for emulsifying and embedding clove essential oil, so that the volatilization loss of the clove essential oil is reduced, and the embedding rate of the clove essential oil reaches 62.91%.

2. Compared with a pure chitosan coating, the tensile strength of the chitosan composite membrane added with the clove essential oil pickering emulsion is improved from 26.15MPa to 38.67 MPa.

3. The clove essential oil pickering emulsion coating preservative has strong bacteriostasis on escherichia coli and staphylococcus aureus, the diameter of a bacteriostasis zone on the escherichia coli is 3.29mm, and the diameter of the bacteriostasis zone on the staphylococcus aureus is 6.15 mm.

4. The application range is wide, and the fruits, vegetables and meat products can be preserved by using the clove essential oil pickering emulsion coating preservative.

Drawings

FIG. 1 is a technical route diagram for preparing a clove essential oil pickering emulsion coating preservative;

FIG. 2 is a graph of mechanical strength of different amounts of clove essential oil Pickering emulsion coating preservative;

FIG. 3 is a scanning electron microscope image of the Pickering emulsion coating preservative with different addition amounts of clove essential oil;

FIG. 4 is a Fourier infrared spectrogram of the Pickering emulsion coating preservative with different addition amounts of clove essential oil;

FIG. 5 is a diameter diagram of inhibition zones of the clove essential oil Pickering emulsion coating preservative for Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) in different addition amounts;

FIG. 6 is a schematic view of a stationary conveyor belt;

FIG. 7 is a diagram showing the control group-example 1 and example 3;

FIG. 8 is a graph showing the total number of colonies during storage of Fuji roast chickens.

Detailed Description

To explain technical contents, structural features, and objects and effects of the technical solutions in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with the embodiments.

Referring to fig. 1, a technical route diagram for preparing a clove essential oil pickering emulsion coating preservative is shown, the invention provides a preparation method of a clove essential oil pickering emulsion coating preservative, which comprises the following steps,

step S1, preparing a zein solution of 20mg/ml by using 70-80% ethanol and zein powder, preparing a sodium caseinate solution of 0.4-6mg/ml by using ultrapure water and sodium caseinate, and magnetically stirring for 6-12 hours;

s2, dropwise adding 1ml of 20mg/ml zein solution into 10ml of 0.4-6mg/ml sodium caseinate solution, and magnetically stirring at 500rpm for 30 min;

step S3, evaporating ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water to ensure that the volume of the solution is not changed;

step S4, adding 0.5-2% v/v clove essential oil, and homogenizing at 15000-.

S5, preparing 1.5% -2% w/v chitosan solution from 1% v/v acetic acid and chitosan, adding clove essential oil pickering emulsion into the chitosan solution to enable the concentration of clove essential oil in the total solution to be 0.2% -0.6% v/v, homogenizing at a high speed of 15000-.

The clove essential oil Pickering emulsion coating preservative prepared by the method of any one technical scheme is provided.

The clove essential oil pickering emulsion coating preservative is applied as a food preservative. Is applied to fruits, vegetables and meat products.

Example 1-control group:

the preparation method of the clove essential oil pickering emulsion coating preservative comprises the following steps:

step S1, preparing 1.5% w/v chitosan solution by 1% v/v acetic acid and chitosan, and adding 20% w/w glycerol into the chitosan solution. Homogenizing at 15000rpm for 3min, and defoaming with ultrasound for 30min to obtain pure chitosan membrane (control group) antistaling agent.

Example 2:

the preparation method of the clove essential oil pickering emulsion coating preservative comprises the following steps:

step S1, preparing a zein solution with the concentration of 75% ethanol and zein being 20 mg/ml; ultrapure water and sodium caseinate are prepared into a 4mg/ml sodium caseinate solution, and the solution is magnetically stirred for 12 hours.

Step S2, dropping 1ml of 20mg/ml zein solution into 10ml of 4mg/ml sodium caseinate solution drop by drop, and magnetically stirring at 500rpm for 30 min.

And step S3, evaporating off ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water to ensure that the volume of the solution is not changed.

And step S4, adding 1% v/v clove essential oil, and homogenizing at 15000rpm for 3min to obtain the clove essential oil pickering emulsion.

Step S5, preparing 1.5% w/v chitosan solution by 1% v/v acetic acid and chitosan, and adding 20% w/w glycerol into the chitosan solution. Then adding the clove essential oil pickering emulsion into the chitosan solution to ensure that the concentration of the clove essential oil in the total solution is 0.2% v/v. Homogenizing at 15000rpm for 3min, and defoaming with ultrasound for 30min to obtain flos Caryophylli essential oil pickering emulsion coating antistaling agent.

Example 3:

the preparation method of the clove essential oil pickering emulsion coating preservative comprises the following steps:

step S1, preparing a zein solution with the concentration of 75% ethanol and zein being 20 mg/ml; ultrapure water and sodium caseinate are prepared into a 4mg/ml sodium caseinate solution, and the solution is magnetically stirred for 12 hours.

Step S2, dropping 1ml of 20mg/ml zein solution into 10ml of 4mg/ml sodium caseinate solution drop by drop, and magnetically stirring at 500rpm for 30 min.

And step S3, evaporating off ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water to ensure that the volume of the solution is not changed.

And step S4, adding 1% v/v clove essential oil, and homogenizing at 15000rpm for 3min to obtain the clove essential oil pickering emulsion.

Step S5, preparing 1.5% w/v chitosan solution by 1% v/v acetic acid and chitosan, and adding 20% w/w glycerol into the chitosan solution. Then adding the clove essential oil pickering emulsion into the chitosan solution to ensure that the concentration of the clove essential oil in the total solution is 0.4% v/v. Homogenizing at 15000rpm for 3min, and defoaming with ultrasound for 30min to obtain flos Caryophylli essential oil pickering emulsion coating antistaling agent.

Example 4:

the preparation method of the clove essential oil pickering emulsion coating preservative comprises the following steps:

step S1, preparing a zein solution with the concentration of 75% ethanol and zein being 20 mg/ml; ultrapure water and sodium caseinate are prepared into a 4mg/ml sodium caseinate solution, and the solution is magnetically stirred for 12 hours.

Step S2, dropping 1ml of 20mg/ml zein solution into 10ml of 4mg/ml sodium caseinate solution drop by drop, and magnetically stirring at 500rpm for 30 min.

And step S3, evaporating off ethanol in the solution at 40 ℃ by using a rotary evaporator, and replacing the evaporated ethanol with ultrapure water to ensure that the volume of the solution is not changed.

And step S4, adding 1% v/v clove essential oil, and homogenizing at 15000rpm for 3min to obtain the clove essential oil pickering emulsion.

Step S5, preparing 1.5% w/v chitosan solution by 1% v/v acetic acid and chitosan, and adding 20% w/w glycerol into the chitosan solution. Then adding the clove essential oil pickering emulsion into the chitosan solution to ensure that the concentration of the clove essential oil in the total solution is 0.6% v/v. Homogenizing at 15000rpm for 3min, and defoaming with ultrasound for 30min to obtain flos Caryophylli essential oil pickering emulsion coating antistaling agent.

Device name and model used:

device name 1: an IKA high-speed shearing disperser; the model is as follows: t18

Device name 2: an ultraviolet spectrophotometer; the model is as follows: UV-6000

Device name 3: a texture analyzer; the model is as follows: TA-XTplus

Device name 4: a constant temperature and humidity chamber; the model is as follows: JC-100-SE

Device name 5: a cold field scanning electron microscope; the model is as follows: SU8920

Device name 6: fourier infrared spectrum analyzer

Device name 7: a sterile homogenizer; the model is as follows: SCIENTZ-09

Centrifuging the clove essential oil pickering emulsion prepared in the step S4 at 3500rpm for 10min to remove any large particles, and then centrifuging at 10000rpm for 30 min. And (3) diluting the supernatant by using absolute ethyl alcohol, and measuring the absorbance of the clove essential oil at the wavelength of 280nm by using a UV-6000 ultraviolet visible spectrophotometer. The embedding rate of the pickering emulsion on the clove essential oil is calculated by comparing the standard curve of the clove essential oil (y is 0.0129x-0.0157, R2 is 0.9992). The experiment result shows that the zein-sodium caseinate nano particles are emulsified and embedded with the clove essential oil, so that the volatilization loss of the clove essential oil is reduced, and the embedding rate of the clove essential oil is 62.91%.

The sample prepared in step S5 was subjected to a tensile test using a device 3 texture analyzer, and the tensile strength and elongation at break of the film were measured. As shown in fig. 2, a graph of mechanical strength of different amounts of the clove essential oil pickering emulsion coating preservative is obtained, and compared with a pure chitosan coating (control tenant) -example 1, the tensile strength of the chitosan composite membrane (0.2% — example 2, 0.4% — example 3, 0.6% — example 4, in sequence from left to right) added with clove essential oil pickering emulsion is increased from 26.15MPa to 38.67MPa, and the elongation at break is increased from 1% to 1.56%.

The membrane was broken in liquid nitrogen, glued onto a conductive gel, sprayed with gold, and the surface and cross-section of the membrane were observed by scanning electron microscopy. As shown in figure 3, which is a scanning electron micrograph of the clove essential oil Pickering emulsion coating antistaling agent with different addition amounts, A1-D1 are the surfaces of example 1, example 2, example 3 and example 4 in sequence, and A2-D2 are the cross sections of example 1, example 2, example 3 and example 4 in sequence. Observed by an electron microscope picture, the clove essential oil pickering emulsion is embedded into the chitosan network structure, and round bulges are arranged on the surface.

And testing the change of the internal molecular structure of the clove essential oil Pickering emulsion coating by a Fourier infrared spectrum analyzer. As shown in figure 4, which is a Fourier infrared spectrogram of the coating antistaling agent of the clove essential oil pickering emulsion with different addition amounts, the chitosan film is kept at 3200cm by adding the clove essential oil pickering emulsion^-1The absorption peak is red-shifted, which means that the clove essential oil pickering emulsion has been tightly bound to chitosan.

According to a flaking diffusion method, gram-negative bacteria, namely escherichia coli, and gram-positive bacteria, namely staphylococcus aureus, are used as test bacteria, and the bacteriostasis of the clove essential oil Pickering emulsion coating preservative on the two food-borne pathogenic bacteria is tested. 100 μ L of cultured 10⁶cfu/g of test bacteria were injected into LB plates and spread evenly. After the flat plate is slightly dried, 6mm sterile blank drug sensitive paper sheets are soaked in the clove essential oil Pickering emulsion coating preservative solution and are uniformly stuck on the flat plate coated with the tested bacteria. The plates were placed in a 37 ℃ incubator for 24-48 h. And measuring the diameter of the bacteriostatic zone by using a vernier caliper. As shown in fig. 5, the diameter of the zone of inhibition of the clove essential oil pickering emulsion coating preservative to escherichia coli (e.coli) and staphylococcus aureus (s.aureus) is shown in the graph, which shows strong bacteriostasis to escherichia coli and staphylococcus aureus, with 0.4% of the clove essential oil pickering emulsion coating preservatives having a zone of inhibition to escherichia coli of 3.29mm and a zone of inhibition to staphylococcus aureus of 6.15 mm.

A method for keeping the freshness of roast chicken by clove essential oil pickering emulsion coating antistaling agent comprises selecting the Shimili Jishao chicken marinated in a Huizhou fragrance source factory, and hanging the Shimili Jishao chicken upside down on a fixed conveyer belt shown in figure 6. The roasted chicken is moved to the upper part of the preservation solution soaking pool through a conveyor belt, and the lifting end of the vertical driving mechanism extends outwards so that the whole fresh roasted chicken is soaked in the preservation solution at the temperature of 0-4 ℃ for 20-60 s. And then the lifting end of the vertical driving mechanism retracts to lift the fresh-burned chickens out of the preservation solution soaking pool, and the fresh-burned chickens are drained in a refrigerating chamber at 4 ℃ and are packed into a fresh-keeping bag for refrigeration at 4 ℃.

As shown in fig. 7, the control group and the final product of example 3 are shown. A0: uncoated roast chicken on day 0 of storage, a 6: roast chicken not coated on day 6 of storage, B0: storage day 0, film-coated roast chicken, B6: and (5) displaying a film-coated roasted chicken finished product on the 6 th day of storage. The clove essential oil emulsion coating is adopted to preserve and store the Fuliji fresh roast chicken, and on the basis of no large-area contact roast chicken, the clove essential oil emulsion coating is safe, non-toxic, free of secondary pollution, green and environment-friendly, so that the original quality of the roast chicken is maintained, and the shelf life of the roast chicken is remarkably prolonged.

Solves the problem of fresh keeping of Fuliji fresh-cooked chicken. The total number of food microbial colonies was determined by GB 4789.2-2016. The finished product prepared in example 3 was sampled every 2 days for 8 days. Taking 10g meat sample in a sterile working table, adding 90mL of normal saline, and beating for 2min for homogenization by an aseptic homogenizer of equipment 1. 100 μ L of the suspension was applied to PCA plates by 10-fold serial dilution and spread evenly. Then inverted and cultured in a constant temperature incubator at 37 ℃ for 24-48 h. As shown in FIG. 8, the total number of colonies during storage of the Fuji roast chicken was plotted. The Shiliji fresh roast chicken sold in the market can be stored only up to the 2 nd day, the total number of bacteria reaches 4.7log cfu/g, namely the requirement of 5log cfu/g of the national standard is exceeded; the Taiji fresh roast chicken coated with the clove essential oil emulsion can be stored for 4-5 days, and the total number of bacteria reaches 5.71log cfu/g on the 6 th day.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the phrases "comprising … …" or "comprising … …" does not exclude the presence of additional elements in a process, method, article, or terminal that comprises the element. Further, herein, "greater than," "less than," "more than," and the like are understood to exclude the present numbers; the terms "above", "below", "within" and the like are to be understood as including the number.

Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes using the contents of the present specification and drawings, or any other related technical fields, which are directly or indirectly applied thereto, are included in the scope of the present invention.