Product quality testing fingerprint spectrum analysis device
阅读说明:本技术 一种产品质检指纹图谱分析装置 (Product quality testing fingerprint spectrum analysis device ) 是由 张萍 刘鸿凌 周永峰 王睿林 牛明 柏兆方 于 2020-06-18 设计创作,主要内容包括:本发明实施例公开了一种产品质检指纹图谱分析装置,包括高效液相色谱仪,控制系统以及设置在所述高效液相色谱仪一侧的自动上样装置,所述自动上样装置用于根据实际检测需要为所述高效液相色谱仪自动提供多种样品,所述自动上样装置包括供样部和取样部,所述高效液相色谱仪设置在所述供样部的侧部,在所述供样部的供样侧设置有壁面处理装置,所述取样部设置在所述壁面处理装置远离所述供样部的一侧,且所述取样部与所述控制系统连接,本发明提供的装置采用机械化取样的方式,在每一次取样前,通过壁面处理装置对取样部进行清洗,以减少取样部上附着的残留物,降低取样瓶之间的交叉污染,提高鉴定结果的准确性。(The embodiment of the invention discloses a product quality inspection fingerprint spectrum analysis device, which comprises a high-efficiency liquid chromatograph, a control system and an automatic sample loading device arranged on one side of the high-efficiency liquid chromatograph, wherein the automatic sample loading device is used for automatically providing various samples for the high-efficiency liquid chromatograph according to actual detection requirements, the automatic sample loading device comprises a sample supplying part and a sampling part, the high-efficiency liquid chromatograph is arranged on the side part of the sample supplying part, a wall surface processing device is arranged on the sample supplying side of the sample supplying part, the sampling part is arranged on one side of the wall surface processing device far away from the sample supplying part, and the sampling part is connected with the control system, the cross contamination among sampling bottles is reduced, and the accuracy of the identification result is improved.)
1. The utility model provides a product quality control fingerprint spectrum analytical equipment, includes high performance liquid chromatograph (1), control system and setting are in automatic loading device (2) of high performance liquid chromatograph (1) one side, automatic loading device (2) are used for according to actual detection needs for high performance liquid chromatograph (1) provides multiple sample, its characterized in that automatically:
automatic loading device (2) is including supplying kind portion (3) and sample portion (4), high performance liquid chromatograph (1) sets up the lateral part of supplying kind portion (3) the confession appearance side of supplying kind portion (3) is provided with wall processing apparatus (5), sample portion (4) set up wall processing apparatus (5) are kept away from one side of supplying kind portion (3), just sample portion (4) with control system connects, it is used for providing a plurality of sample bottles (6) that are equipped with the sample to supply kind portion (3), sample portion (4) pass under control system control wall processing apparatus (5) are followed sample bottle (6) extract the sample, wall processing apparatus (5) are used for carrying out the preliminary treatment to the sample portion (4) of process in order to avoid polluting sample bottle (6).
2. The product quality inspection fingerprint analysis device according to claim 1, wherein the sampling part (4) comprises a sampling needle (401) connected with the high performance liquid chromatograph (1) through a pipeline, and a driving device (402) for driving the sampling needle (401) to move, wherein the driving device (402) is connected with the control system;
the sample supplying part (3) comprises a containing plate (301), and the containing plate (301) is used for containing a plurality of sample bottles (6) with different samples;
the wall surface treatment device (5) comprises a pre-treatment cavity (501) and a pre-treatment device (502) arranged at the bottom of the pre-treatment cavity (501);
the driving device (402) is used for driving the sampling needle (401) to penetrate through the front processing cavity (501), different samples placed in the containing plate (301) are extracted to the high performance liquid chromatograph (1) to be detected, and the front processing device (502) is used for cleaning the sampling needle (401) penetrating through the front processing cavity (501).
3. The product quality control fingerprint analysis device according to claim 2, wherein the pre-processing device (502) is connected with an external fluid circulation device (7) through a pipeline to form a circulation structure.
4. A product quality control fingerprinting apparatus according to claim 3 characterized in that an adaptive closing part (503) is arranged at both ends of the pre-processing cavity (501), the sampling needle (401) penetrates the pre-processing cavity (501) through the adaptive closing part (503);
the self-adaptive closing piece (503) comprises a connecting through hole (504), a sealing plug (505) for closing the connecting through hole (504) is screwed in the connecting through hole (504) through threads, and a guide block (506) which is screwed in the connecting through hole (504) through threads and used for guiding the sampling needle (401) is arranged on one side, facing the sampling needle (401), of the sealing plug (505);
the sealing plug (505) comprises a threaded sleeve (507) matched with the connecting through hole (504), and sealing rubber (508) is filled in the threaded sleeve (507).
5. The product quality inspection fingerprint analysis device according to claim 3, wherein the pre-processing device (502) comprises a gaseous medium flow distribution plate (509) and a liquid medium flow distribution plate (510) which are both positioned at the bottom of the pre-processing chamber (501), and the liquid medium flow distribution plate (510) is positioned above the gaseous medium flow distribution plate (509);
the fluid circulating device (7) comprises a gaseous medium circulating device (701) and a liquid medium circulating device (702), the gaseous medium flow distribution plate (509) is connected with the gaseous medium circulating device (701) through a pipeline, the liquid medium flow distribution plate (510) is connected with the liquid medium circulating device (702) through a pipeline, and the liquid medium flow distribution plate (510) and the liquid medium flow distribution plate (509) are respectively used for spraying liquid medium and gas medium into the front processing cavity (501) to perform impact cleaning on the side wall of the sampling needle (401).
6. The product quality inspection fingerprint analysis device according to claim 5, wherein the liquid medium flow distribution plate (510) is a hollow structure, and a plurality of liquid discharge holes (511) are formed in one side of the liquid medium flow distribution plate (510) opposite to the gaseous medium flow distribution plate (509);
one side of the gaseous medium flow distribution plate (509) facing the liquid medium flow distribution plate (510) is provided with a plurality of exhaust heads (512) in a shape structure, a one-way airtight component (513) is arranged in each exhaust head (512), and each one-way airtight component (513) is used for preventing the liquid medium from being poured into the gaseous medium flow distribution plate (509).
7. The product quality inspection fingerprint analysis device according to claim 6, wherein the one-way airtight assembly (513) comprises an annular stopper (514) disposed in the venting head (512), and a sealing plate (515) for sealing the venting head (512) by engaging the annular stopper (514) is connected by a spring to a side of the annular stopper (514) away from the gaseous medium flow distribution plate (509).
8. The product quality inspection fingerprint analysis device according to claim 3, wherein a separation chamber (8) for separating liquid medium and gaseous medium is connected to one side of the pre-processing chamber (501) through a pipeline, the top of the separation chamber (8) is connected to the gaseous medium circulation device (701) through a pipeline, and the bottom of the separation chamber (8) is connected to the liquid medium circulation device (702) through a pipeline.
9. The product quality inspection fingerprint spectrum analysis device according to claim 3, characterized in that a plurality of irregularly distributed connecting ropes (516) are drawn in the front processing cavity (501), a plurality of irregularly distributed turbulence blocks (517) are arranged on the connecting ropes (516), and the turbulence blocks (517) are used for disturbing the flow tracks of the liquid medium and the gas medium.
10. An analysis method based on the product quality inspection fingerprint analysis device of any one of claims 1 to 9, characterized by comprising the following steps:
step 100, storing a unique number in a control system for each containing plate, and when each containing plate is filled with a prepared sample bottle, recording sample information in the sample bottle corresponding to the number of each containing plate in the control system;
200, when identifying the sample, controlling the driving device to drive the sampling needle to move to the corresponding sample bottle for sampling through the operation control system according to the identification sequence of the sample;
step 300, during sampling, the sampling needle penetrates through the pre-treatment cavity, and fluid inside the side wall of the pre-treatment cavity respectively performs cleaning treatment on the outer side wall of the sampling needle;
step 400, presetting a distance value for pausing when the sampling needle enters the preposed processing cavity in the control system, and when the sampling needle pauses, controlling the negative pressure mechanism by the control system to enable the sampling needle to absorb the fluid in the preposed processing cavity and push out again for several times;
step 500, the control system controls the driving device to enable the sampling needle to continuously penetrate through the pre-processing cavity to enter the sampling bottle for sampling, and the sampling needle is sent into the high performance liquid chromatograph to finish primary sampling;
and step 500, circulating the steps 200 to 400 until the sampling and the identification of all the samples are completed.
Technical Field
The embodiment of the invention relates to the technical field of fingerprint spectrums, in particular to a product quality inspection fingerprint spectrum analysis device.
Background
The fingerprint refers to a chromatogram or a spectrogram which can mark chemical characteristics of certain complex substances, such as traditional Chinese medicines, DNA of certain organisms or certain tissues or cells, obtained by adopting a certain analysis means after protein is properly processed, and the fingerprint is mainly divided into a traditional Chinese medicine fingerprint, a DNA fingerprint and a peptide fingerprint.
The traditional Chinese medicine fingerprint refers to a chromatogram or a spectrogram which can mark the chemical characteristics of certain traditional Chinese medicines or traditional Chinese medicine preparations by adopting a certain analysis means after the traditional Chinese medicines or the traditional Chinese medicine preparations are properly processed. The traditional Chinese medicine fingerprint is a comprehensive and quantifiable identification means and is mainly used for evaluating the authenticity, the excellence and the stability of the quality of traditional Chinese medicine and semi-finished products of traditional Chinese medicine preparations.
The existing acquisition of traditional Chinese medicine fingerprint is mostly realized through a high performance liquid chromatograph, the types of traditional Chinese medicine samples needing to be identified in practice are more, the traditional Chinese medicine samples comprise different components of the same raw material, different components of different raw materials and the like, and the identification process needs to be carried out through the comparison of the fingerprints of the traditional Chinese medicine samples of different types, so that more identification information is acquired.
The traditional Chinese medicine identification is mostly carried out manually through a dropper or other disposable tools for sampling traditional Chinese medicine samples, but for various samples, the sampling operation needs to be carried out manually on most sample containers, and in the sampling process, the following steps need to be completed almost simultaneously: the method comprises the steps of approving the sample information in the sampling container, carrying out sampling operation by holding the sampling container and the sampling tool, controlling the sampling amount, extracting to the high performance liquid chromatograph, placing the sampling container after sampling and the sampling tool.
Therefore, the existing operation mode has complicated steps, is very easy to make mistakes, and has low sampling precision, if tools such as a dropper are adopted, each sampling needs to be cleaned once in sequence, and substances attached to the surface of the sampling tool cannot be completely cleaned, so that the problem of sample pollution exists when different samples are sampled, and the accuracy of a test result is caused.
Disclosure of Invention
Therefore, the embodiment of the invention provides a product quality inspection fingerprint spectrum analysis device, which aims to solve the problems that in the prior art, most of sampling needles are cleaned by cleaning liquid, and the inner walls of the sampling needles are cleaned by cleaning liquid, and the detected samples are easily polluted due to the existence of sample residues on the side walls when a plurality of samples are taken.
In order to achieve the above object, an embodiment of the present invention provides the following:
a product quality inspection fingerprint spectrum analysis device comprises a high performance liquid chromatograph, a control system and an automatic sample loading device arranged on one side of the high performance liquid chromatograph, wherein the automatic sample loading device is used for automatically providing various samples for the high performance liquid chromatograph according to actual detection requirements;
the automatic sample loading device comprises a sample feeding portion and a sampling portion, the high performance liquid chromatograph is arranged on the side portion of the sample feeding portion, a wall surface processing device is arranged on the sample feeding side of the sample feeding portion, the sampling portion is arranged on the wall surface processing device and is far away from one side of the sample feeding portion, the sampling portion is connected with the control system, the sample feeding portion is used for providing a plurality of sample bottles filled with samples, the sampling portion penetrates under the control of the control system, the wall surface processing device is used for extracting the samples from the sample bottles, and the wall surface processing device is used for pre-processing the sampling portion passing through so as to avoid polluting the sample bottles.
As a preferable scheme of the present invention, the sampling part comprises a sampling needle connected with the high performance liquid chromatograph through a pipeline, and a driving device for driving the sampling needle to move, wherein the driving device is connected with the control system;
the sample supplying part comprises a containing plate, and the containing plate is used for containing a plurality of sample bottles with different samples;
the wall surface treatment device comprises a pre-treatment cavity and a pre-treatment device arranged at the bottom of the pre-treatment cavity;
the driving device is used for driving the sampling needle to penetrate through the front processing cavity and extracting different samples in the containing plate to the high performance liquid chromatograph for detection, and the front processing device is used for cleaning the sampling needle penetrating through the front processing cavity.
As a preferable scheme of the invention, the pretreatment device is connected with an external fluid circulating device through a pipeline to form a circulating structure.
As a preferable scheme of the invention, adaptive closing parts are arranged at two ends of the front treatment cavity, and the sampling needle penetrates through the front treatment cavity through the adaptive closing parts;
the self-adaptive closing piece comprises a connecting through hole, a sealing plug for closing the connecting through hole is screwed in the connecting through hole through threads, and a guide block which is screwed in the connecting through hole through threads and used for guiding the sampling needle is arranged on one side, facing the sampling needle, of the sealing plug;
the sealing plug comprises a threaded sleeve matched with the connecting through hole, and sealing rubber is filled in the threaded sleeve.
As a preferable scheme of the invention, the pre-treatment device comprises a gaseous medium flow distribution plate and a liquid medium flow distribution plate which are both positioned at the bottom of the pre-treatment cavity, and the liquid medium flow distribution plate is positioned above the gaseous medium flow distribution plate;
the fluid circulating device comprises a gaseous medium circulating device and a liquid medium circulating device, the gaseous medium splitter plate is connected with the gaseous medium circulating device through a pipeline, the liquid medium splitter plate is connected with the liquid medium circulating device through a pipeline, and the liquid medium splitter plate and the gaseous medium splitter plate are respectively used for spraying a liquid medium and a gas medium into the pre-treatment cavity to perform impact cleaning on the side wall of the sampling needle.
As a preferred scheme of the invention, the liquid medium splitter plate is of a hollow structure, and a plurality of liquid discharge holes are formed in one side of the liquid medium splitter plate, which is opposite to the gaseous medium splitter plate;
one side of the gaseous medium flow distribution plate, which faces the liquid medium flow distribution plate, is provided with a plurality of exhaust heads in a shape like a Chinese character 'ji', and a one-way airtight assembly is arranged in each exhaust head and is used for preventing the liquid medium from flowing backwards into the gaseous medium flow distribution plate.
In a preferred embodiment of the present invention, the one-way airtight assembly includes an annular stopper disposed in the exhaust head, and a sealing plate for sealing the exhaust head in cooperation with the annular stopper is connected to a side of the annular stopper away from the gaseous medium flow distribution plate through a spring.
As a preferable scheme of the present invention, a separation chamber for separating a liquid medium and a gaseous medium is connected to one side of the pre-treatment chamber through a pipeline, the top of the separation chamber is connected to a gaseous medium circulation device through a pipeline, and the bottom of the separation chamber is connected to a liquid medium circulation device through a pipeline.
As a preferable scheme of the present invention, a plurality of irregularly distributed connecting ropes are pulled in the pre-treatment chamber, and a plurality of irregularly distributed turbulence blocks are arranged on the connecting ropes and used for disturbing flow tracks of the liquid medium and the gas medium.
An analysis method of a product quality inspection fingerprint spectrum analysis device comprises the following steps:
step 100, storing a unique number in a control system for each containing plate, and when each containing plate is filled with a prepared sample bottle, recording sample information in the sample bottle corresponding to the number of each containing plate in the control system;
200, when identifying the sample, controlling the driving device to drive the sampling needle to move to the corresponding sample bottle for sampling through the operation control system according to the identification sequence of the sample;
step 300, during sampling, the sampling needle penetrates through the pre-treatment cavity, and fluid inside the side wall of the pre-treatment cavity respectively performs cleaning treatment on the outer side wall of the sampling needle;
step 400, presetting a distance value for pausing when the sampling needle enters the preposed processing cavity in the control system, and when the sampling needle pauses, controlling the negative pressure mechanism by the control system to enable the sampling needle to absorb the fluid in the preposed processing cavity and push out again for several times;
step 500, the control system controls the driving device to enable the sampling needle to continuously penetrate through the pre-processing cavity to enter the sampling bottle for sampling, and the sampling needle is sent into the high performance liquid chromatograph to finish primary sampling;
and step 500, circulating the steps 200 to 400 until the sampling and the identification of all the samples are completed.
The embodiment of the invention has the following advantages:
the device provided by the invention adopts a mechanical sampling mode, and the sampling part is cleaned by the wall surface processing device before each sampling, so that residues attached to the sampling part are reduced, the cross contamination among sampling bottles is reduced, and the accuracy of an identification result is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a schematic overall structure diagram of an embodiment of the present invention;
FIG. 2 is a schematic view of an installation structure of a pre-processing apparatus according to an embodiment of the present invention;
fig. 3 is a schematic perspective view of a sealing plug according to an embodiment of the present invention;
fig. 4 is a schematic structural view of a liquid medium flow distribution plate according to an embodiment of the present invention;
FIG. 5 is a schematic structural view of a gaseous medium flow distribution plate in an embodiment of the present invention;
FIG. 6 is an enlarged view of a portion of the structure at A in FIG. 2;
fig. 7 is an enlarged view of a portion of the structure at B in fig. 5.
In the figure:
1-high performance liquid chromatograph; 2-automatic sample loading device; 3-a sample supply part; 4-a sampling section; 5-a wall surface treatment device; 6-sample bottle; 7-a fluid circulation device; 8-a separation chamber;
301-holding plate;
401-a sampling needle; 402-a drive device;
501-a pre-processing chamber; 502-preprocessing unit; 503-adaptive closure; 504-connecting vias; 505-a sealing plug; 506-a guide block; 507-threaded sleeves; 508-sealing rubber; 509-gaseous medium diverter plate; 510-liquid medium flow distribution plate; 511-drain hole; 512-exhaust head; 513-one-way airtight component; 514-annular stop block; 515-sealing plate; 516-a connecting rope; 517-a turbulence block;
701-a gaseous medium circulation device; 702-liquid medium circulation device.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 7, the present invention provides a product quality inspection fingerprint analysis apparatus, which includes a high performance liquid chromatograph 1, a control system and an automatic sample loading device 2 disposed on one side of the high performance liquid chromatograph 1, wherein the automatic sample loading device 2 is configured to automatically provide multiple samples for the high performance liquid chromatograph 1 according to actual detection requirements, and the control system is mainly configured to control the automatic sample loading device 2 so as to automatically extract the samples into the high performance liquid chromatograph 1 for detection.
Automatic loading device 2 is including supplying
The analytical equipment that this embodiment provided mainly realizes automatic sample to and handle sample portion before sampling each time, one avoids different sample bottles to cause cross contamination at the sampling in-process.
The
In the actual use of
The
As shown in fig. 1, 2 and 6, the wall surface processing device 5 includes a pre-processing chamber 501 and a pre-processing device 502 disposed at the bottom of the pre-processing chamber 501, the driving
Leading processing apparatus 502 is connected through pipeline and external
The two ends of the pre-processing cavity 501 are provided with adaptive closing parts 503, the
The
The adaptive closing member 503 is mainly used to close the pre-processing chamber 501, so as to avoid the problem of leakage of the fluid medium when flowing through the pre-processing chamber 501, and at the same time, the
As shown in fig. 2 and fig. 3, the sealing plug 505 includes a threaded sleeve 507 matching with the connection through
As shown in fig. 2, 4, 5, and 6, the pre-processing apparatus 502 includes a gaseous medium
The gaseous medium
The
Gaseous medium
In the cleaning process, the gaseous medium rises in the liquid medium in the form of a small bubble, and the small bubbles are combined into a large bubble when contacting the
In addition, the liquid medium can be preferably distilled water, the gaseous medium can be preferably air, and the user can make corresponding adjustment according to the actual situation.
Draw in leading processing chamber 501 and be equipped with a plurality of
The connecting
The turbulence block 8 is irregular, and when the sampling probe is used, the flow of the liquid medium and the gaseous medium is influenced by the turbulence block 8, so that the liquid medium and the gaseous medium can further move irregularly in the pre-treatment cavity 501, and the cleaning effect of the
The liquid medium
As shown in fig. 2, 5 and 7, a plurality of exhaust heads 512 in a zigzag structure are disposed on one side of the gaseous medium
The exhaust end of the
The one-way airtight assembly 513 includes an
When the
As shown in fig. 2, a separation chamber 8 for separating a liquid medium and a gaseous medium is connected to one side of the pre-treatment chamber 501 through a pipe, the top of the separation chamber 8 is connected to the gaseous
The separation cavity 8 is used for recycling the gaseous medium and the fluid medium passing through the pre-treatment cavity 501, when in use, the gaseous medium and the fluid medium can uniformly enter the separation cavity 8 through a pipeline, and because the density of the gaseous medium is lower than that of the liquid medium, the liquid medium can be concentrated at the bottom of the separation cavity 8, and the gaseous medium is concentrated at the top of the separation cavity 8, so that the gaseous medium and the liquid medium can be separated and enter corresponding circulation for recycling or recycling.
It should be noted that when the liquid medium is recycled, the recovered liquid medium needs to be filtered to avoid the problem of secondary pollution during recycling, and when the gaseous medium is recycled, the liquid medium also needs to be treated correspondingly.
An analysis method of a product quality inspection fingerprint spectrum analysis device comprises the following steps:
step 100, storing a unique number in a control system for each containing plate, and when each containing plate is filled with a prepared sample bottle, recording sample information in the sample bottle corresponding to the number of each containing plate in the control system;
200, when identifying the sample, controlling the driving device to drive the sampling needle to move to the corresponding sample bottle for sampling through the operation control system according to the identification sequence of the sample;
step 300, during sampling, the sampling needle penetrates through the pre-treatment cavity, and fluid inside the side wall of the pre-treatment cavity respectively performs cleaning treatment on the outer side wall of the sampling needle;
step 400, presetting a distance value for pausing when the sampling needle enters the preposed processing cavity in the control system, and when the sampling needle pauses, controlling the negative pressure mechanism by the control system to enable the sampling needle to absorb the fluid in the preposed processing cavity and push out again for several times;
step 500, the control system controls the driving device to enable the sampling needle to continuously penetrate through the pre-processing cavity to enter the sampling bottle for sampling, and the sampling needle is sent into the high performance liquid chromatograph to finish primary sampling;
and step 500, circulating the steps 200 to 400 until the sampling and the identification of all the samples are completed.
Can improve
Meanwhile, in order to improve the utilization rate of the fluid medium, a sensor for detecting the solubility of the fluid medium can be arranged in the front treatment cavity, namely, when the fluid medium can be recycled by impurities in the fluid medium within a safety value range, otherwise, a user is reminded of carrying out timely replacement, and corresponding adjustment can be carried out according to actual requirements.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.