Formulations for administration of RNA
阅读说明：本技术 用于施用rna的制剂 (Formulations for administration of RNA ) 是由 乌尔·沙欣 海因里希·哈斯 安内特·福格尔 斯特凡妮·埃尔巴尔 克斯廷·瓦尔策 安妮·施莱 于 2019-01-10 设计创作，主要内容包括：本发明涉及包含聚合复合物制剂的组合物,其用于在肠胃外施用之后(特别是在肌内施用之后)将RNA递送至靶器官或靶细胞。更确切地说,本发明涉及用于特别是通过肌内注射来施用RNA(例如自复制RNA)的制剂。更详细地,所述制剂包含来自单链RNA和聚亚烷基亚胺的聚合复合物颗粒。(The present invention relates to compositions comprising a polymeric complex formulation for delivering RNA to a target organ or target cell following parenteral administration, particularly following intramuscular administration. More specifically, the invention relates to formulations for administering RNA (e.g., self-replicating RNA), particularly by intramuscular injection. In more detail, the formulation comprises polymeric complex particles from single stranded RNA and polyalkyleneimine.)
1. A pharmaceutical composition comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkyleneimine.
2. A composition comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
the composition is for use as a medicament.
3. The composition according to claim 1 or 2, wherein the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the single-stranded RNA (N: P ratio) is from 2.0 to 15.0, preferably from 6.0 to 12.0.
4. A composition comprising:
(a) single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the single-stranded RNA (N: P ratio) is from 2.0 to 15.0, preferably from 6.0 to 12.0.
5. The composition according to any one of claims 1 to 4, wherein the ionic strength is 50mM or less, preferably wherein the concentration of monovalent cations is 25mM or less and the concentration of divalent cations is 20 μ M or less.
6. A composition comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the ionic strength is 50mM or less.
7. The composition according to claim 6, wherein the concentration of monovalent cations is 25mM or less and the concentration of divalent cations is 20 μ M or less.
8. The composition of any one of claims 1 to 7, wherein the single-stranded self-replicating RNA is a cis replicon.
9. The composition of any one of claims 1-8, wherein the single-stranded self-replicating RNA is derived from Venezuelan Equine Encephalitis Virus (VEEV).
10. The composition of claim 9, wherein the single-stranded self-replicating RNA corresponds to or substantially corresponds to a genomic RNA of VEEV or an attenuated form thereof, wherein the open reading frame encoding a structural protein is replaced by the open reading frame encoding the antigen-or epitope-containing peptide or protein.
11. The composition of claim 9 or 10, wherein the antigen or epitope-containing peptide or protein is a membrane protein, such as a viral envelope protein.
12. The composition of any one of claims 9 to 11, wherein the antigen is influenza hemagglutinin.
13. The composition of any one of claims 1 to 8, wherein the single-stranded self-replicating RNA is derived from Semliki Forest Virus (SFV).
14. The composition of claim 13, wherein the single-stranded self-replicating RNA corresponds to or substantially corresponds to a genomic RNA of SFV or an attenuated form thereof, wherein the open reading frame encoding a structural protein is replaced with the open reading frame encoding the antigen-or epitope-containing peptide or protein.
15. The composition of claim 13 or 14, wherein the antigen or the peptide or protein comprising an antigen or epitope is not a membrane protein.
16. The composition of any one of claims 13-15, wherein the antigen is a viral capsid protein.
17. The composition according to any one of claims 1 to 16, for intramuscular administration, e.g. by intramuscular injection.
18. The composition of any one of claims 1 to 17, wherein the single stranded RNA and the polyalkyleneimine are present in a polymeric complex particle.
19. The composition of any one of claims 1 to 18, wherein the polyalkyleneimine comprises the following general formula (I):
wherein
R is H, acyl or a group comprising the following general formula (II):
wherein R is1Is H or a group comprising the following general formula (III):
n, m and l are independently selected from integers from 2 to 10; and is
p, q and r are integers, wherein the sum of p, q and r is such that the average molecular weight of the polymer is 1.5-102To 107Da, preferably 5000 to 105Da, more preferably 10000 to 40000Da, more preferably 15000 to 30000Da, even more preferably 20000 to 25000 Da.
20. The composition of claim 19, wherein n, m and l are independently selected from 2, 3, 4 and 5, preferably from 2 and 3.
21. The composition of claim 19 or 20, wherein R1Is H.
22. The composition of any one of claims 19 to 21, wherein R is H or acyl.
23. The composition according to any one of claims 1 to 22, wherein the polyalkyleneimine comprises a polyethyleneimine and/or a polypropyleneimine, preferably a polyethyleneimine.
24. The composition of any one of claims 1 to 23, wherein at least 92% of the N atoms in the polyalkyleneimine are protonatable.
25. The composition of any one of claims 1 to 24, further comprising one or more additives.
26. The composition of claim 25, wherein the one or more additives are selected from the group consisting of buffering substances, sugars, stabilizers, cryoprotectants, lyoprotectants, and chelating agents.
27. The composition of claim 26, wherein the buffering substance comprises at least one selected from the group consisting of: 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid (HEPES), 2- (N-morpholino) ethanesulfonic acid (MES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), acetic acid, acetate buffers and the like, phosphoric acid and phosphate buffers, and citric acid and citrate buffers.
28. The composition of claim 26 or 27, wherein the saccharide comprises at least one selected from the group consisting of: monosaccharides, disaccharides, trisaccharides, oligosaccharides and polysaccharides, preferably selected from glucose, trehalose and sucrose.
29. The composition of any one of claims 26 to 28, wherein the cryoprotectant comprises at least one selected from the group consisting of: glycols, such as ethylene glycol, propylene glycol, and glycerol.
30. The composition of any one of claims 26 to 29, wherein the chelating agent comprises EDTA.
31. The composition of any one of claims 1 to 30, wherein the composition comprises HEPES Buffered Glucose (HBG), MES Buffered Glucose (MBG), acetate buffered glucose, or HEPES Buffered Trehalose (HBT).
32. The composition according to any one of claims 18 to 31, wherein the z-average from dynamic light scattering measurements of the particles is less than 200nm, preferably less than 150nm, and more preferably less than 100nm, and/or the polydispersity index from dynamic light scattering measurements of the particles is less than 0.5, preferably less than 0.3, and more preferably less than 0.2.
33. The composition of any one of claims 18 to 32, wherein the zeta potential of the particles is 20mV or higher, preferably 25 to 40 mV.
34. The composition according to any one of claims 31 to 33, wherein the HBG comprises 5% glucose (w/v) and 10mM HEPES, pH 7.1.
35. The composition of any one of claims 31 to 33 wherein the HBT comprises 10% trehalose (w/v) and 10mM HEPES, pH 7.1.
36. The composition of any one of claims 18 to 35, wherein the particle is neutral or positively charged at physiological pH.
37. The composition according to any one of claims 1 to 36, wherein the single stranded RNA is a molecule of 6000 to 15000 bases, preferably 9000 to 12000 bases.
38. A composition according to any one of claims 1 to 37 for use in therapy.
39. The composition of any one of claims 1 to 38, which is a vaccine composition.
40. The composition of any one of claims 1 to 39 for use in inducing an immune response.
41. The composition of claim 40, which is administered by intramuscular administration.
42. A method of inducing an immune response comprising the step of administering the composition of any one of claims 1 to 40.
43. The method of claim 42, wherein the composition is administered by intramuscular administration.
44. The composition of claim 40 or 41 or the method of claim 42 or 43, wherein the immune response is directed against the antigen or epitope.
Technical Field
The present invention relates to compositions comprising a polymeric complex (polyplex) formulation for delivering RNA to a target organ or cell following parenteral administration, in particular following intramuscular administration. More specifically, the invention relates to formulations for administering RNA (e.g., self-replicating RNA), particularly by intramuscular injection. In more detail, the polymeric complex particle comprises single-stranded RNA (preferably self-replicating or self-amplifying RNA) and polyalkyleneimine (polyalkyleneimine). The RNA may encode a protein of interest, such as a pharmaceutically active protein. The RNA is taken up by the cell and the RNA is preferably translated into a peptide or protein that can exhibit its physiological activity. The compositions of the invention are suitable for inducing or enhancing an immune response. It may also be used for the prophylactic and/or therapeutic treatment of diseases involving antigens (e.g. proteins). Furthermore, the present invention relates to a method for producing a stable composition comprising an RNA-polymeric complex formulation comprising single stranded RNA and a polyalkyleneimine. The RNA-polymer complex particle formulations described herein can be frozen and thawed, or dehydrated and rehydrated without a loss of product quality, particularly without a significant loss of RNA activity. In particular, the RNA-polymer complex particle formulations described herein can be frozen or dehydrated by freeze-drying, spray-drying, or related methods, thereby enabling an extended product shelf life (shelf life) relative to liquid storage. Furthermore, the RNA-polymer complex particle formulations described herein may meet the requirements of pharmaceutical products, more specifically with respect to the requirements of GMP production and the quality requirements of pharmaceutical products for parenteral administration. The RNA-polymer complex formulations described herein are particularly useful for vaccination of humans or animals, e.g. against infectious diseases.
Background
The introduction of exogenous nucleic acids encoding one or more polypeptides for prophylactic and therapeutic purposes has been the goal of biomedical research for many years. Common to the prior art methods is the delivery of nucleic acid molecules to a target cell or organism, but the type of nucleic acid molecule and/or delivery system differs: ribonucleic acid (RNA) molecules have received increasing attention in recent years, subject to safety concerns associated with the use of deoxyribonucleic acid (DNA) molecules. Various methods have been proposed, including the administration of single-stranded or double-stranded RNA in the form of naked RNA or in complexed or packaged form (e.g., in a non-viral or viral delivery vehicle). In viruses and in viral delivery vehicles, nucleic acids are typically encapsulated by proteins and/or lipids (viral particles). For example, engineered RNA viral particles derived from RNA viruses have been proposed as delivery vehicles for the treatment of plants (WO 2000/053780A2) or for vaccination of mammals (Tubulikas et al, 1997, Gene, Vol.190, pp.191-195). In view of safety concerns, the medical and veterinary community is reluctant to administer RNA viral particles to humans or animals. Non-viral delivery vehicles applicable to RNA have been extensively studied for the development of gene-based delivery therapeutics. However, the conversion of non-viral gene delivery methods to clinical practice is not very successful for a variety of reasons. Reasons may be associated with unsatisfactory gene expression levels, technical and regulatory issues related to drug development of such complex products, and safety reasons.
Thus, there is a need for a pharmaceutical product for the safe and efficient delivery of RNA encoding proteins of therapeutic value (e.g., vaccines) in patients and animals. As described herein, some aspects and embodiments of the present invention address this need.
Summary of The Invention
Immunotherapeutic strategies represent a promising option for the prevention and treatment of, for example, infectious and cancer diseases. The identification of an increasing number of pathogen-associated and tumor-associated antigens has led to the widespread collection of suitable targets for immunotherapy. The present invention comprises improved reagents and methods useful for the high expression of antigens, for the immunotherapeutic treatment of diseases, both prevention and treatment.
In one aspect, the present invention relates to a pharmaceutical composition comprising:
(a) single-stranded RNA; and
(b) a polyalkyleneimine.
In another aspect, the present invention relates to a composition comprising:
(a) single-stranded RNA; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
the composition is for use as a medicament.
In one embodiment of all aspects of the invention, the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the single-stranded RNA (N: P ratio) is from 1.0 to 30, preferably from 2.0 to 15.0, more preferably from 6.0 to 12.0.
In another aspect, the present invention relates to a composition comprising:
(a) single-stranded RNA; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the single-stranded RNA (N: P ratio) is 1.0 to 30.0, preferably 2.0 to 15.0, more preferably 6.0 to 12.0.
In one embodiment of all aspects of the invention, the ionic strength of the composition is 50mM or less, preferably wherein the concentration of positively charged monovalent ions is 25mM or less and the concentration of free positively charged divalent cations is 20. mu.M or less.
In another aspect, the present invention relates to a composition comprising:
(a) single-stranded RNA; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the ionic strength is 50mM or less.
In one embodiment, the concentration of the positively charged monovalent ion is 25mM or less and the concentration of the positively charged divalent cation is 20. mu.M or less.
In one embodiment of all aspects of the invention, the composition is for intramuscular administration, e.g. by intramuscular injection.
In one embodiment of all aspects of the invention, said single stranded RNA and said polyalkyleneimine are present in a polymeric complex particle.
In one embodiment of all aspects of the invention, the polyalkyleneimine comprises the following general formula (I):
wherein
R is H, acyl or a group comprising the following general formula (II):
wherein R is1Is H or a group comprising the following general formula (III):
n, m and 1 are independently selected from integers from 2 to 10; and is
p, q and r are integers, wherein the sum of p, q and r is such that the average molecular weight of the polymer is 1.5-102To 107Da, preferably 5000 to 105Da, more preferably 10000 to 40000Da, more preferably 15000 to 30000Da, even more preferably 20000 to 25000 Da.
In one embodiment, n, m and 1 are independently selected from 2, 3, 4 and 5, preferably from 2 and 3. In one embodiment, R1Is H. In one embodiment, R is H or acyl.
In one embodiment of all aspects of the invention, the polyalkyleneimine comprises a polyethyleneimine and/or a polypropyleneimine, preferably a polyethyleneimine.
In one embodiment of all aspects of the invention, at least 92% of the N atoms in the polyalkyleneimine are protonatable.
In one embodiment of all aspects of the invention, the composition of the invention comprises one or more additives. In one embodiment, the one or more additives are selected from the group consisting of buffering substances, sugars, stabilizers, cryoprotectants, lyoprotectants, and chelating agents. In one embodiment of all aspects of the invention, the composition of the invention comprises one or more polymers. In one embodiment, the buffer substance comprises at least one selected from the group consisting of: 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid (HEPES), 2- (N-morpholino) ethanesulfonic acid (MES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), acetic acid buffer systems and the like, phosphoric acid buffer systems or citric acid buffer systems. In one embodiment of all aspects of the invention, the composition of the invention comprises a buffer for buffering in the pH range of 4 to 8 (preferably 5 to 7.5). Some examples of such buffer systems are acetate buffers or HEPES buffers or phosphate buffers or acetate buffers. In one embodiment, the saccharide comprises at least one selected from the group consisting of: monosaccharides, disaccharides, trisaccharides, oligosaccharides and polysaccharides, preferably selected from glucose, trehalose, sucrose and dextran. In one embodiment, the additive is dextran having an average molar mass of 1kDa to 100 kDa. In one embodiment, the cryoprotectant comprises at least one selected from the group consisting of: glycols, such as ethylene glycol, propylene glycol, and glycerol. In one embodiment, the chelating agent comprises EDTA. In one embodiment, the lipid comprises at least one selected from the group consisting of: cationic lipids, neutral lipids, and anionic lipids. In one embodiment, the composition of the present invention comprises one or more block copolymers comprising ethylene oxide and propylene oxide building blocks. In one embodiment, the composition of the invention comprises a copolymer containing ethylenediamine groups. In one embodiment, the composition of the invention comprises an amphiphilic block copolymer, preferably comprising ethylene oxide and propylene oxide building blocks, optionally also comprising ethylenediamine groups.
In one embodiment of all aspects of the invention, the composition comprises HEPES buffered glucose (HBG or hbgx 1), MES-buffered glucose (MBG or mbgx 1), Acetate Buffered Glucose (ABG), or HEPES buffered trehalose (HBT or hbgx 1). In one embodiment of all aspects of the invention, the composition comprises glucose or trehalose or sucrose in an acetate buffer at a concentration of 0.1mM to 10 mM. In one embodiment of all aspects of the invention, the composition comprises glucose or trehalose or sucrose in a phosphate buffer at a concentration of 0.1mM to 10 mM.
In one embodiment of all aspects of the invention, the z-average size of the particles is less than 200nm, preferably less than 150nm, and more preferably less than 100 nm. In one embodiment, the z-average size of the particles is from 50nm to 200 nm. In one embodiment of all aspects of the invention, the zeta potential of the particles is 20mV or more, preferably from 25 to 40 mV. In one embodiment of all aspects of the invention, the particles have an electrophoretic mobility (μ) of 1 to 1.6 μm cm/V S. In one embodiment of all aspects of the invention, the z-average size and/or zeta potential and/or electrophoretic mobility of the particles is determined in a suspension comprising the polymer composite particles and HEPES Buffered Glucose (HBG) or HEPES Buffered Trehalose (HBT). In one embodiment, the HBG comprises 5% glucose (w/v) and 10mM HEPES, pH 7.1, or the HBT comprises 10% trehalose (w/v) and 10mM HEPES, pH 7.1. In one embodiment, the z-average size of the particles is determined by dynamic light scattering and data analysis by a cumulant algorithm (cumulant algorithm). In one embodiment, the migration diffusion coefficient (transition diffusion coefficient) is measured by dynamic light scattering. Then, the Stock-Einstein equation was used to calculate the Z-average. In one embodiment, electrophoretic mobility is measured by laser-Doppler electrophoresis (laser-Doppler electrophoresis). Then, the Henry equation or Smoluchowski equation was used to calculate the zeta potential.
In one embodiment of all aspects of the invention, the particles are preferably neutral or positively charged at physiological pH or at a pH of 4.5 to 7.5.
In one embodiment of all aspects of the invention, the single stranded RNA is a molecule of 6000 to 15000 bases, preferably 9000 to 12000 bases. In one embodiment of all aspects of the invention, the single stranded RNA encodes at least one protein of interest. In one embodiment of all aspects of the invention, the single stranded RNA is a replicon, preferably a self-replicating or self-amplifying RNA. In one embodiment, the replicon is replicable by replicase from alphavirus (alphavirus), and wherein the replicon preferably comprises a5 'replication recognition sequence from alphavirus or a variant thereof and a 3' replication recognition sequence from alphavirus or a variant thereof. In one embodiment of all aspects of the invention, the single stranded RNA comprises an open reading frame encoding a peptide or protein of interest (e.g. a pharmaceutically active peptide or protein).
In one embodiment of all aspects of the invention, the compositions described herein are used in therapy. In one embodiment of all aspects of the invention, the composition described herein is a vaccine composition.
In another aspect, the invention relates to the use of a composition as described herein for introducing RNA into a cell, in particular for expressing RNA in a cell. In one embodiment, the cell is a muscle cell.
In another aspect, the invention relates to the use of a composition described herein for intramuscular administration of RNA.
In another aspect, the invention relates to a method of administering RNA intramuscularly comprising the step of administering intramuscularly the composition described herein.
In another aspect, the present invention relates to a frozen, lyophilized or spray-dried composition comprising:
(a) single-stranded RNA; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the composition comprises a cryoprotectant and/or a lyoprotectant, preferably a disaccharide (e.g. trehalose) or a polysaccharide (e.g. dextran).
In one embodiment, the composition further comprises a chelating agent (e.g., EDTA).
In one embodiment, the composition is prepared from an aqueous composition (aqueous composition) comprising 5% to 20% (w/v) of a disaccharide and optionally 20 μ M to 10mM (e.g., 80 μ M to 5mM) of the chelating agent. In one embodiment, the aqueous composition comprises trehalose, HEPES and EDTA, e.g., 10% trehalose (w/v), 2.8 mh epes, 80 μ M EDTA, pH 7.1.
In another aspect, the present invention relates to an aqueous composition obtainable by thawing a frozen composition as described herein or reconstituting a lyophilized or spray-dried composition as described herein.
In another aspect, the present invention relates to a method of preparing a frozen, lyophilized or spray-dried composition comprising:
(i) preparing an aqueous composition comprising single stranded RNA, a polyalkyleneimine and a cryoprotectant and/or lyoprotectant, preferably a disaccharide (e.g. trehalose) or a polysaccharide (e.g. dextran), and
(ii) freezing, lyophilizing or spray drying the composition.
In one embodiment, the aqueous composition further comprises a chelating agent (e.g., EDTA). In one embodiment, the aqueous composition comprises 5% to 20% (w/v) disaccharide and optionally 20 μ M to 10mM (e.g. 80 μ M to 5mM) chelating agent. In one embodiment, the aqueous composition comprises trehalose, HEPES and EDTA, e.g., 10% trehalose (w/v), 2.8mM HEPES, 80 μ M EDTA, pH 7.1.
In another aspect, the present invention relates to the use of a cryoprotectant and/or lyoprotectant, preferably a disaccharide (e.g. trehalose) or a polysaccharide (e.g. dextran), for the preparation of a frozen, lyophilized or spray-dried composition comprising:
(a) single-stranded RNA; and
(b) a polyalkyleneimine.
In one embodiment, the disaccharide is used in combination with a chelating agent (e.g., EDTA).
A frozen or lyophilized or spray-dried composition or an aqueous composition for preparing a frozen or lyophilized or spray-dried composition, which may comprise one or more of the following:
(i) non-aqueous solvents such as ethylene glycol, glycerol, dimethyl sulfoxide and dimethylformamide.
(ii) Surfactants such as
(iii) Disaccharides, such as trehalose, sucrose, lactose and maltose.
(iv) Polymers (which may have different MW) such as polyethylene glycol, dextran, poly (vinyl alcohol), hydroxypropyl methylcellulose, gelatin, polyvinylpyrrolidone, hydroxyethyl cellulose, Ficoll and albumin.
(v) Amino acids such as glycine, proline, 4-hydroxyproline, L-serine, glutamic acid, alanine, lysine, sarcosine and gamma-aminobutyric acid.
In another aspect, the present invention relates to a method for continuous flow preparation of an RNA polymerization complex preparation by using a continuous flow pump (continuous flow pump) and a mixing device, wherein two aqueous fluids are mixed through a mm or μm sized channel.
Brief Description of Drawings
FIG. 1.A. toxicity of free pure PEI on HEK-293 cells in vitro. IC (integrated circuit)5077 μ M nitrogen (free). Toxicity of PEI/replicon-RNA polymerization complexes on HEK-293 cells in vitro. IC (integrated circuit)50542 μ M nitrogen (polymer complex formulation).
FIG. 2 relative luminescence of C2C12 myocytes after incubation with PEI/replicon-RNA polymeric complexes with an N/P of 11.6 after 1 week of storage at different storage conditions.
FIG. 3 relative RNA integrity of PEI/replicon-RNA polymerization complexes with an N/P of 11.6 after 2 weeks of storage at different storage conditions.
FIG. 4. by 2-ethyl-2-ion in the presence of an initiator
FIG. 5 Synthesis of fully deacylated Linear PEI22, PEI87 and PEI217 by acid hydrolysis of PEOZ. Conditions are as follows: (i) 24% (wt/vol) HCl, 110 ℃, 96 hours; n-504 for 50-kDa PEOZ, n-2,018 for 200-kDa PEOZ, and n-5,044 for 500-kDa PEOZ.
FIG. 6 aggregation kinetics of IVT (A) and replicon (B) polymerization complexes at increasing salt concentrations.
FIG. 7 physicochemical parameters of the polymeric composite before (initial) and after (final) filtration. A and b. the diameter and polydispersity of the polymeric composite was measured by DLS. C. RNA was released from the polymer complex by heparin and measured by UV absorption at 260 nm. D. By CuSO4The assay measures PEI concentration.
FIG. 8 is a comparison of the chemical structures of high purity PEI and conventional purity PEI. For 25kDa PEI, n is 58. In PEI25kD, the average number of-CH 2CH2 NH-monomers was 581, which is also the length of the continuous segment of potentially protonatable nitrogen (contiguoustretch). Assuming a uniform distribution of the N-propionyl moieties in conventional PEI25, the continuous fraction of protonatable nitrogens is only 64.
FIG. 9 replicon-RNA polymerization complexes of different N/P ratios prepared by using PEI of different purity levels were used to transfect C2C12 muscle cells in vitro.
FIG. 10 preparation of replicon-RNA polymerization complexes having N/P ratios of 1(-) and 11.6(+) using high purity PEI (jetPEI) and conventional purity PEI (25 kDa). Free RNA was used as control. Formulation i.m. was injected into the hind limb of mice (n ═ 3). Luminescence signals from the mouse muscle were recorded.
FIG. 11 replicon-RNA polymerization complexes with N/P ratios of 7.7 and 11.6 were prepared with the following high purity PEI: jetPEI (from Polyplus), PEI-Max 40000 (from Polyscience) and Exgen 500 (from Eurodamex). All formulations were prepared in
FIG. 12 Freeze-dried cake of JetPEI/replicon-RNA polymerization complex (lyophilized cake) with N/P11.6 prepared with different buffers.
FIG. 13C 2C12 myocytes were transfected in vitro with IVT-RNA encoding luciferase. RNA was complexed with JetPEI in HBPx1 buffer at different N/P ratios. Luminescence signals were measured 24 hours after transfection.
FIG. 14 IVT-RNA polymerization complexes with N/P ratios of 5.8 and 11.6 were prepared with pure PEI in
FIG. 15 preparation of polymeric complexes of replicon-RNA and jetPEI at different RNA concentrations in
FIG. 16 transfection of C2C12 myocytes in vitro by the polymeric complex from FIG. 16. Luminescence signals were measured 24 hours after transfection.
FIG. 17 Rep-RNA polymerization complexes with N/P ratio of 11.6 were prepared with pure PEI in
FIG. 18 in vitro studies of the action of PEI/replicon-RNA polymerization complexes on human Dendritic Cells (DCs) and mouse myocytes (C2C 12). A. Toxicity (expressed as% of viable cells after treatment with polymeric complexes). B. Transfection (expressed as luminescence emission after treatment with the polymeric complex). Only the transfection results for C2C12 cells are shown.
FIG. 19 Rep-RNA polymerization complexes with N/P ratios of 11.6 or 15.8 were prepared with PEI from Polyplus or polytheraene in
FIG. 20.A) animals were imaged for non-invasive in
Figure 21.a) animals were imaged for non-invasive in
FIG. 22 beneficial effects of replicon-RNA formulations as vaccines.
FIG. 23 beneficial effects of replicon-RNA formulations as vaccines.
FIG. 24 results from spray drying of replicon-RNA formulated with PEI in 10% (w: v) trehalose.
FIG. 25 normalized luminescence from C2C12 muscle cells after incubation with PEI/replicon-RNA polymeric complexes at different N/P ratios before (prepared) and after (sterilized) sterile filtration.
FIG. 26: effect of the combination of short PEI and long PEI on transfection efficiency in vitro according to example 16. Fig. 26A): transfection efficiency of short linear PEI and long PEI polymeric complexes at 250ng RNA/well. Fig. 26B): transfection efficiency of short-branched PEI and long PEI polymeric complexes at 250ng RNA/well. Using a combination of short PEI (FIG. 26A: linear; FIG. 26B: branched) and long PEI (e.g. in vivo JetPEI) achieves higher expression levels within different time frames (time frames) compared to the baseline in vivo Jet PEI and for the same total NP ratio.
FIG. 27 is a schematic view showing: according to example 17, replicon-RNA transfection efficiency of the long Jet PEI + short PEI polymeric complex relative to a benchmark (i.e. in vivo JetPEI NP 12): for a total NP of 12, short PEI (branched 1.8kDA) and long PEI (NP4+ NP8 or NP1.15+ NP11) in different combinations.
FIG. 28: according to example 18, the effect of salt changes (e.g.NaCl) on the transfection efficiency of replicon (saRNA) -RNA in vivo. Bioluminescence signals were detected on day 3 (fig. 28A), day 6 (fig. 28B), day 9 (fig. 28C) and day 13 (fig. 28D). The signal strengths are compared in fig. 28E. The most intense signal in the mouse muscle region was detectable at
FIG. 29: effect of pH adjustment on transfection efficiency of replicon (saRNA) -PEI formulations according to example 18. Good results were obtained with saRNA-PEI polymeric complex formulations with pH 6.5 to 7.1. The most intense signal was detected with the saRNA-long PEI NP12 formulation adjusted to pH 6.5. As a basis, saRNA-Jet PEI polymerized complex NP12(BM) or HBG (20mM Hepes, pH 7.4, 5 wt.% glucose) without pH adjustment was used.
FIG. 30: electrophoretic mobility of in vivo JetPEI/replicon-RNA polymerization complexes (N/P4) adjusted to different pH values according to example 19.
FIG. 31: according to example 19, normalized luminescence from C2C12 myocytes after incubation with different doses of in vivo JetPEI/replicon-RNA polymerization complexes with N/P ratio of 4 and different pH values (pH 6.5 to pH 8.5).
FIG. 32: luciferase expression after transfection with different amounts of PEI/excess positive charge in PEI preparations, according to example 20.
FIG. 33: according to example 21, the polymer complex transfection was optimized by using 2-step complexation (complexation).
FIG. 34: the immunization experiment according to example 22 showed an excellent effect of saRNA-polymeric complex formulated with MES Buffered Glucose (MBG) compared to HEPES Buffered Glucose (HBG).
FIG. 35: following intramuscular (i.m.) immunization with PEI formulated self-amplifying RNA (saRNA) encoding HA of a/California/7/2009(H1N1) virus (HA of the a/California/7/2009(H1N1) virus, H1N1/Cf7-HA), animals developed neutralizing antibody immune responses.
(A) BALB/c mice were immunized once on
(B) On
The group of animals receiving the formulated VEEV-saRNA vaccine developed an immune response similar to animals injected with the positive control. SFV-sarRNA also resulted in the development of neutralizing antibody immune responses, but titers were lower than after VEEV-sarRNA immunization. Mean ± SEM are shown in the figure.
FIG. 36: following intramuscular (i.m.) immunization with self-amplifying RNA (saRNA) encoding Porcine Circovirus 2 (
BALB/c mice were immunized twice with buffer, 1. mu.g of SFV-sarRNA encoding PCV2-cap _ EU or VEEV-sarRNA formulated with PEI at a 12/1N/P ratio on
A similar antibody response against PCV2-cap _ EU protein occurred in the group of animals receiving the formulated SFV-or VEEV-sarRNA vaccine. The antibody immune response after a single vaccination with SFV-sarRNA was slightly higher than the antibody immune response after a single vaccination with VEEV-sarRNA. After two immunizations, the antibody responses of both types of saRNA vaccines were almost identical. Mean ± SEM are shown in the figure.
FIG. 37: replicon RNA (sarRNA) was complexed as N/P12 under different buffer systems and pH conditions. Both types of buffers, acetate or MES buffer, contained a buffer at a final concentration of 10mM and D-glucose at a final concentration of 5% w/v. The saRNA/PEI-polyplex was stored in each buffer at 4 ℃ for different time periods (1, 2, 4 and 8 days after complexation). After complexing the different formulations, RNA integrity was measured directly (t ═ 0). RNA integrity was measured by capillary electrophoresis. After incubation for 20 minutes at RT with a strong excess of a polyanion that induces electrostatic interaction with the polymer, releasing the RNA enclosed in the polymeric complex, saRNA complexed in the polymeric complex can be released. 200ug of released RNA was used for capillary electrophoresis assay strictly following the protocol provided with the appropriate kit (standard sensitivity RNA assay kit DF 471). For each time point, the reference saRNA was used to quantify saRNA integrity.
Higher pH values in the formulation buffer resulted in significantly increased degradation of the saRNA. Minimal loss of saRNA integrity after complexation was achieved with acetate buffer at
FIG. 38: the PEI formulated sarRNA-VEEV encoding HA of A/California/7/2009(
BALB/c mice were i.m. immunized twice on
Detailed Description
Although the invention is described in detail below, it is to be understood that this invention is not limited to the particular methodology, protocols, and reagents described herein as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention, which will be limited only by the appended claims. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art.
Preferably, the terms used herein are defined as described in the following: "A multilingulosysary of biotechnology terms: (IUPAC Recommendations), "h.g.w.leuenberger, b.nagel, and H.
The practice of the present invention will employ, unless otherwise indicated, conventional methods of chemistry, biochemistry, cell biology, immunology and recombinant DNA techniques which are explained in the literature of the art (see, e.g., molecular cloning: A Laboratory Manual, 2 nd edition, edited by J.Sambrook et al, Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989).
The elements of the present invention will be described below. These elements are listed with some specific embodiments, however, it is understood that they may be combined in any manner and in any number to produce additional embodiments. The various described examples and preferred embodiments should not be construed as limiting the invention to only the explicitly described embodiments. This description should be understood to disclose and cover embodiments that combine the explicitly described embodiments with any number of the disclosed and/or preferred elements. Moreover, any arrangement or combination of elements described in this application is intended to be disclosed herein unless otherwise indicated by the context.
The term "about" means about or near, and in the case of a numerical value or range recited herein, preferably means +/-10% of the numerical value or range recited or claimed.
The terms "a" and "an" and "the" and similar referents used in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., "such as") provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention.
The term "comprising" is used in the context of this document to indicate that, unless otherwise explicitly stated, there may optionally be additional members other than the members of the list introduced by "comprising". However, it is contemplated that as a specific embodiment of the present invention, the term "comprising" covers the possibility that no other member is present, i.e. for this purpose, the embodiment "comprising" is to be understood as having the meaning "consisting of …".
Throughout this specification text, several documents are cited. Each of the documents cited herein (including all patents, patent applications, scientific publications, manufacturer's specifications, instructions, etc.), whether supra or infra, is hereby incorporated by reference in its entirety. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention.
Definitions applicable to all aspects of the invention are provided below.
As used herein, terms such as "reduce" or "inhibit" mean the ability to cause an overall reduction in levels of preferably 5% or more, 10% or more, 20% or more, more preferably 50% or more, and most preferably 75% or more. The term "inhibit" or similar phrases include complete or substantially complete inhibition, i.e., reduction to zero or substantially to zero.
Terms such as "increase" or "enhancement" preferably relate to an increase or enhancement of about at least 10%, preferably at least 20%, preferably at least 30%, more preferably at least 40%, more preferably at least 50%, even more preferably at least 80%, and most preferably at least 100%.
With respect to nucleic acid sequences, "fragments" relate to a portion of a nucleic acid sequence, i.e. a sequence representing a nucleic acid sequence shortened at the 5 '-and/or 3' -end. Preferably, a fragment of a nucleic acid sequence comprises at least 80%, preferably at least 90%, 95%, 96%, 97%, 98% or 99% of the nucleotide residues from said nucleic acid sequence. In the present invention, those fragments of the RNA molecule that retain RNA stability and/or translation efficiency are preferred.
With respect to amino acid sequences (peptides or proteins), "fragments" relate to a part of an amino acid sequence, i.e. a sequence representing an amino acid sequence shortened at the N-terminus and/or the C-terminus. A fragment shortened at the C-terminus (N-terminal fragment) can be obtained, for example, by translation of a truncated open reading frame lacking the 3' end of the open reading frame. A fragment shortened at the N-terminus (C-terminal fragment) may be obtained, for example, by translation of a truncated open reading frame lacking the 5' end of the open reading frame, as long as the truncated open reading frame comprises a start codon to initiate translation. A fragment of an amino acid sequence comprises, e.g., at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90% of the amino acid residues from the amino acid sequence.
The term "ionic strength" refers to the mathematical relationship between the number of different species of ionic species in a particular solution and their respective charges. Therefore, the ionic strength I is mathematically represented by the following formula
Where c is the molar concentration of the particular ionic species and z is the absolute value of its charge. The sum sigma is taken from all the different species of ions (i) in the solution.
The ionic strength of the compositions described herein is preferably 50mM or less, preferably 25mM or less, preferably 20mM or less, 19mM or less, 18mM or less, 17mM or less, 16mM or less, 15mM or less, 10mM or less or 5mM or less. Preferably, the ionic strength of the compositions described herein is sufficiently low to prevent aggregation of the polymer composite particles.
According to the invention, the term "ionic strength" preferably relates to the presence of monovalent ions. With respect to the presence of divalent ions (in particular divalent cations), their concentration or effective concentration (presence of free ions) is preferably low enough to prevent degradation of the RNA due to the presence of the chelating agent. In a particularly preferred embodiment, the concentration or effective concentration of the divalent ion is below the catalytic level for hydrolysis of phosphodiester bonds between RNA nucleotides. In a particularly preferred embodiment, the concentration of free divalent ions is 20 μ M or less, preferably no or substantially no free divalent ions are present.
The pH of the compositions described herein is preferably from 4 to 8; more preferably from 5.5 to 8, such as from 6 to 7.5, for example from 6.5 to 7.1, from 6.5 to 7 or from 6.5 to 6.9.
The term "disaccharide" refers to a carbohydrate composed of two monosaccharide residues linked by a glycosidic bond. Some representative examples of disaccharides include: trehalose, maltose, sucrose, lactose, lactulose, cellobiose, isomaltose, gentiobiose, laminaribiose (laminaribiose), chitobiose, xylobiose (xylobiose), inulinbiose and mannobiose. Preferred levels of disaccharides in the compositions described herein are 5% to 20% (w/v), such as 5% to 15% (w/v), 7% to 15% (w/v), or 8% to 12% (w/v). Disaccharides with a high glass transition temperature are preferred according to the invention.
The term "chelating agent" means a compound that forms a chelate with a metal ion, preferably a divalent or polyvalent metal ion. The chelating agent has a plurality of groups capable of forming a ring structure with the metal ion, for example, OH, -COOH. Some examples of chelating agents are: ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), trans-1, 2-diamino-cyclohexanetetraacetic acid monohydrate, N-hydroxyethylethylenediaminetriacetic acid (HEDTA), citric acid, and phosphoric acid chelating agents (e.g., Dequest 2000). According to the invention, ethylenediaminetetraacetic acid (EDTA) is preferred. Preferably, the chelating agent is present in the compositions described herein at a concentration of at least 20 μ M, at least 40 μ M, at least 60 μ M or at least 80 μ M. Preferably, the chelating agent is present in the compositions described herein at a concentration of at most 10mM, at most 5mM, at most 2mM, at most 1mM, at most 0.5mM, at most 0.2mM or at most 0.1 mM.
The term "freezing" relates to solidification of a liquid, usually accompanied by removal of heat.
The term "lyophilization" refers to freeze-drying of a substance by freezing the substance and then reducing the ambient pressure so that the freezing medium in the substance sublimes directly from a solid phase to a gas phase.
The term "spray drying" refers to spray drying of a substance by mixing a (heated) gas with a fluid that is atomized (sprayed) within a vessel (spray dryer), wherein the solvent from the droplets formed evaporates, producing a dry powder.
The term "cryoprotectant" relates to a substance added to a formulation to protect an active ingredient during the freezing phase.
The term "lyoprotectant" relates to a substance added to a formulation to protect an active ingredient during the drying phase.
The term "reconstitution" relates to the addition of a solvent (e.g. water) to a dry product to bring it back to a liquid state, e.g. its original liquid state.
The term "autologous" is used to describe anything that originates from the same subject. For example, "autologous cells" refer to cells derived from the same subject. Introducing autologous cells into a subject is advantageous because these cells overcome the immune barrier that would otherwise result in rejection.
The term "allogenic" is used to describe anything derived from different individuals of the same species. When the genes at one or more loci are not identical, two or more individuals are considered allogeneic to each other.
The term "isogenic" is used to describe anything derived from individuals or tissues having the same genotype (i.e., animals of the same egg twin or same inbred strain, or tissues or cells thereof).
The term "heterologous" is used to describe something consisting of a plurality of different elements. For example, cells from one individual may be introduced into a different individual to form an allograft. A heterologous gene is a gene derived from a source other than the subject.
According to the present invention, it is advantageous to provide RNA molecules in complexed form due to the instability of unprotected RNA. In particular, in some embodiments, the compositions of the present invention comprise particles comprising RNA and polyalkyleneimine.
When the system according to the invention is formulated as a particle formulation, each RNA species (e.g. replicon, replicase construct and optionally further RNA species (e.g. RNA encoding a protein suitable for inhibiting IFN)) may be formulated independently as a separate particle formulation. In this case, each individual particle preparation will contain one RNA species. The separate particle formulations may be present as separate entities, for example in separate containers. Such a formulation may be obtained by providing each RNA species (typically each in the form of a solution comprising RNA) separately together with a particle-forming agent, thereby allowing the formation of particles. Each particle will only contain the specific RNA species provided at the time of particle formation (individual particle preparation).
In one embodiment, the composition according to the invention comprises more than one separate granular formulation. Each composition is referred to as a mixed particulate formulation. The mixed granule formulation according to the present invention may be obtained by independently forming individual granule formulations as described above and then mixing the individual granule formulations. Through the mixing step, a preparation comprising a mixed population of RNA-containing particles is obtained (e.g., a first population of particles can comprise a replicon, and a second population of particles can comprise a replicase construct, for example). The individual particle populations may be together in a container containing a mixed population of the individual particle formulations.
Alternatively, all RNA species of the composition (e.g., the replicon, replicase construct, and optional additional species (e.g., RNA encoding proteins suitable for inhibiting IFN)) may be formulated together into a combined particle formulation. Such a formulation may be obtained by providing a combined formulation (typically a combined solution) of all RNA species together with the particle-forming agent, thereby allowing particle formation. In contrast to mixed particle preparations, combined particle preparations will typically comprise particles containing more than one RNA species. In a combined particle composition, different RNA species are typically present together in a single particle.
In one embodiment, the particle formulation of the present invention is a nanoparticle formulation. In this embodiment, the composition according to the invention comprises RNA in the form of nanoparticles.
In the general definition, the term "nanoparticle" refers to any particle having a diameter of 1nm to 1000 nanometers (nm).
In the context of the present invention, the term "particle" relates to a structured entity formed by molecules or molecular complexes. In one embodiment, the term "particle" relates to a micro-or nano-sized structure, such as a micro-or nano-sized dense structure.
The terms "In vivo-jetPEITM", "In vivo jetPEI", "jet PEI" and "jetPEI" all refer to the commercially available In vivo-jetPEITM reagent from Polyplus-Transfection SA (Illkirch, France), # 201-50G.
The term "polymeric complex" as used herein refers to a complex formed by electrostatic interaction between a polymer and a nucleic acid (e.g., RNA). Where the polymeric complex comprises RNA, it may also be referred to as an "RNA complex" or an "RNA polymeric complex".
The present invention relates to polymeric complex particles formed from at least one single-stranded RNA and at least one polyalkyleneimine.
In one embodiment, the average diameter of the particles described herein is less than about 200nm, preferably less than about 150nm, and more preferably less than about 100 nm. In one embodiment, the average diameter of the particles described herein is at least about 30nm, at least about 40nm, at least about 50nm, at least about 60nm, at least about 70nm, at least about 80nm, at least about 90nm, or at least about 100 nm.
The term "average diameter" refers to the measurement and use by dynamic light scatteringThe cumulant algorithm of (a) provides a mean hydrodynamic diameter of the particle for data analysis, which provides a so-called Z with a length dimension and a dimensionless Polydispersity Index (PI)Mean value ofAs a result (Koppel, d., j.chem.phys.57, 1972, pages 4814-4820, ISO 13321). Here the "average diameter", "diameter" or "size" of the particles and the ZMean value ofThe values of (A) are used synonymously.
The term "net charge" relates to the sum of charges (e.g., positive and negative). For example, if a particle contains a higher number of negative charges than positive charges, the net charge of the particle is negative. If the particle contains a higher number of positive charges than negative charges, the net charge of the particle is positive. If the particles contain equal amounts of positive and negative charges, the net charge of the particles is neutral, in particular electrically neutral. Thus, the net charge of the particles according to the invention may be negative, positive or neutral. In one embodiment, the net charge of the particle is positive. In one embodiment, the net charge of the particle is negative.
The terms "charged," "net charge," "negatively charged," or "positively charged" refer to the electrical net charge of a given compound or particle when dissolved or suspended in an aqueous buffer at a relevant pH (e.g., 7.1).
According to the invention, the terms "N/P ratio", "NP ratio", "N: P ratio", "N/P" and "NP" refer to the molar ratio of nitrogen atoms (N) in the polyethyleneimine to phosphorus atoms (P) in the RNA.
According to the invention, the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the RNA (N/P ratio) is preferably from 2.0 to 15.0, preferably from 8.0 to 12.0, from 6.0 to 14.0 or from 6.0 to 12.0.
According to the invention, the composition described herein is preferably adjusted to the final N/P ratio in more than 1 step (e.g. in 2, 3, 4 or more steps). For example, the composition may be adjusted in a first step to a first N/P ratio that is lower than the final N/P ratio, for example using a long polyalkyleneimine. The N/P ratio can be adjusted to the final N/P ratio by adding additional polyalkyleneimines (e.g., short polyalkyleneimines or long polyalkyleneimines, such as the long polyalkyleneimines used in the first step). In one embodiment, the final N/P ratio is from 8 to 16, such as from 9 to 14, such as from 10 to 12. In one embodiment, the N/P ratio produced in the first step is from 1 to 6, such as from 2 to 5 (e.g., 3 or 4).
Polyalkyleneimines
The polyalkyleneimines used herein preferably comprise the following general formula (I):
wherein
R is H, acyl or a group comprising the following general formula (II):
wherein R is1Is H or a group comprising the following general formula (III):
n, m and 1 are independently selected from integers from 2 to 10; and is
p, q and r are integers, where the sum of p, q and r is such that the average molecular weight of the polymer is 1.5-102To 107Da, preferably 5000 to 105Da, more preferably 10000 to 40000Da, more preferably 15000 to 30000Da, even more preferably 20000 to 25000 Da.
In one embodiment, n, m and 1 are independently selected from 2, 3, 4 and 5, preferably from 2 and 3, and more preferably 2. In one embodiment, R1Is H. In one embodiment, R is H or acyl.
In one embodiment, the polyalkyleneimines comprise polyethyleneimines and/or polypropyleneimines, preferably polyethyleneimines. A preferred polyalkyleneimine is Polyethyleneimine (PEI). The average molecular weight of PEI is preferably 1.5. multidot.102To 107Da, preferably 5000 to 105Da, more preferably 10000 to 40000Da, more preferably 15000 to 30000Da, even more preferably 20000 to 25000 Da.
Preferred according to the invention are linear polyalkyleneimines, such as linear Polyethyleneimine (PEI). In one embodiment, linear PEI is obtained as follows: 2-ethyl-2-Oxazoline cycloisomerization polymerization to obtain poly (2-ethyl-2-)
Preferably, according to the present invention, linear PEI is obtained by complete or substantially complete deacylation of PEOX. For example, PEOX with a molecular weight of 50kDa provides linear PEI with a molecular weight of 22 kDa. Preferably, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or essentially 100% of the substituents on the nitrogen atoms in the polyalkyleneimine (e.g., polyethyleneimine) are hydrogen (i.e., R in the above formula is H). Thus, preferably, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or essentially 100% of the nitrogen atoms in a polyalkyleneimine, such as a polyethyleneimine, are protonatable.
Preferred polyalkyleneimines according to the present invention are Polyethyleneimines (PEI), in particular linear polyethyleneimines. Such linear polyethylenimine preferably has a molar mass of from 15kDa to 30kDa and is preferably used in combination with self-replicating or self-amplifying RNA, wherein the N/P ratio is preferably of from 6 to 15, and the linear polyethylenimine and the self-replicating or self-amplifying RNA are preferably present in polymeric complex particles having a size of less than 200nm, preferably less than 150nm, and even more preferably less than 100 nm.
In one embodiment of the invention the polyalkyleneimine is a combination of a short polyalkyleneimine, for example a short polyethyleneimine (linear and/or branched) of 0.6 to 11kDa, preferably 1 to 6kDa or1 to 4kDa (e.g. 1 to 3kDa) with a long polyalkyleneimine (e.g. a long polyethyleneimine (linear and/or branched) of 20 to 40 kDa), wherein the total N/P ratio is preferably 8 to 16, for example 9 to 14, for example 10 to 12 in one embodiment the N/P ratio of the long polyalkyleneimine and RNA is 1 to 6, for example 2 to 5, for example 3 or 4.
Polyethyleneimine (PEI) is an organic macromolecule with high cationic charge density. PEI compresses nucleic acids into positively charged particles that are able to interact with anionic proteoglycans on the cell surface and facilitate entry of the particles by endocytosis.
For PEI, there are several manufacturing methods. According to the present invention, linear polyethyleneimines are preferably synthesized and prepared by a process comprising the steps of: from a defined amount of monomeric 2-ethyl-2-carboxylic acid with a purity higher than 99%Starting with oxazoline, thoroughly drying said amount of monomer and polymerizing said amount of monomer to obtain poly (2-ethyl-2-Oxazoline) (PEOX):
-after thoroughly drying a predetermined amount of acetonitrile, using said acetonitrile as solvent in said amount of dried monomer while adding a predetermined amount of thoroughly dried polymerization initiator and mixing them together,
-purifying the obtained PEOX by evaporation to remove the solvent, while carrying out at least three successive washing/precipitation steps with methanol and ether and corresponding filtration,
arranging said drying, polymerization and purification operations to obtain (i) by carrying out1H NMR testing, correct identification of the PEOX polymer, confirmation of absence of monomer to a level < 1.0% and confirmation of absence of solvent to a level < 5.0%; and (ii) by performing Gel Permeation Chromatography (Gel Permeation Chromatography), said PEOX having a molecular weight (Mw) average of > 23,000Da and a polydispersity (Mw/Mn) < 1.5,
-salts ofAcid hydrolyzing said PEOX to obtain said PEI with sufficient efficiency by performing1H-NMR measurements, the amount of residual side chains or propionic acid was < 5% and the PEI was identified as a single peak.
It will be appreciated that by thoroughly drying a specific amount of monomer, acetonitrile or initiator, a reduction of the moisture of the water below 10ppm is obtained just before use, which can be obtained by drying on calcium hydride for 48 hours and subsequently by distilling and collecting the monomer at a temperature above 129 ℃.
According to the invention, one or more of the following features are preferred:
(i) the average molecular weight (Mw) of PEOX is, for example, 40,000Da < Mw < 60,000 Da;
(ii) a monomer/initiator ratio of about 500 (about should be understood as ± 5%);
(iii) a monomer/initiator ratio of 480;
(iv) the purity of the monomer is higher than 99.95 percent;
(v) mixing an initiator with acetonitrile prior to addition to the monomers;
(vi) the polymerization is carried out at a temperature higher than 85 ℃ for more than 20 hours;
(vii) the temperature of the polymerization is higher than or equal to 105 ℃;
(viii) after the first filtration, the residue is washed freely with a solvent (e.g. MeOH), and after addition of diethyl ether, the poly (2-ethyl-2-Oxazoline) from the solution to separate naturally as an oil, decanting off all solvents and repeating the washing and separation at least four times, followed by drying in vacuo;
(ix) the hydrolysis step comprises removing from the reaction mixture the propionic acid which is expelled, obtained by azeotropic distillation on a regular basis and during at least one day, and simultaneously1H-NMR spectrum is used for monitoring the reaction process;
(x) The residue obtained at the end of the reaction process was diluted in water and evaporated at least three times to remove traces of propionic acid, then the residue was redissolved in water and filtered before lyophilization;
(xi) Filtration is provided by a sterile membrane (in particular a sterile cellular acetate membrane) having a mesh size of 0.20 μm to 0.25 μm.
Advantageously, the linear PEI used according to the invention is characterized in that the molecular weight Mw of the intermediate PEOX is, for example, 40,000 < Mw < 60,000 Da.
The degree of polymerization is controlled by the monomer/initiator ratio and the yield of the synthesis. Molecular weight determination can be performed by Gel Permeation Chromatography (GPC).
The term "nucleic acid" according to the present invention includes deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and Locked Nucleic Acid (LNA). Nucleic acids according to the invention include genomic DNA, cDNA, mRNA, viral RNA, recombinantly prepared and chemically synthesized molecules. According to the invention, the nucleic acid may be in the form of a single-stranded or double-stranded and linear or covalently closed circular molecule. The term "nucleic acid" according to the present invention also includes chemical derivatization of nucleic acids at the nucleotide base, at the sugar or at the phosphate, as well as nucleic acids comprising non-natural nucleotides and nucleotide analogs. The nucleic acid may be an isolated and/or recombinant nucleic acid.
The term "isolated" as used herein is intended to refer to a molecule that is substantially free of other molecules (e.g., other cellular material). The term "isolated nucleic acid" according to the present invention means that the nucleic acid has been (i) amplified in vitro, e.g., by Polymerase Chain Reaction (PCR), (ii) recombinantly produced by cloning, (iii) purified, e.g., fractionated by cleavage and gel electrophoresis, or (iv) synthesized, e.g., by chemical synthesis. An isolated nucleic acid is a nucleic acid that can be used for manipulation by recombinant techniques.
In the context of the present invention, the term "recombinant" means "prepared by genetic engineering". Preferably, a "recombinant substance" is not naturally occurring in the context of the present invention.
The term "naturally occurring" as used herein refers to the fact that an object (object) can be found in nature. For example, peptides or nucleic acids that are present in organisms (including viruses) and that can be isolated from sources in nature and that have not been intentionally modified by man in the laboratory are naturally occurring. The term "found in nature" means "occurring in nature" and includes known guests as well as guests that have not been found and/or isolated from nature but may be found and/or isolated from a natural source in the future.
"nucleic acid sequence" according to the invention refers to a sequence of nucleotides in a nucleic acid, such as ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The term can refer to the entire nucleic acid molecule (e.g., a single strand of the entire nucleic acid molecule) or a portion (e.g., a fragment) thereof.
"3' end of a nucleic acid" according to the invention refers to the end having a free hydroxyl group. In the illustration of double-stranded nucleic acids (particularly DNA), the 3' end is always on the right side. "5' end of a nucleic acid" according to the invention refers to the end having a free phosphate group. In the illustration of double-stranded nucleic acids (particularly DNA), the 5' end is always on the left hand side.
5 'terminal 5' - -P-NNNNNNNNN-OH-3 '3' terminal
3′-HO-NNNNNNN-P--5′
"upstream" describes the relative positioning of a first element of a nucleic acid molecule relative to a second element of the nucleic acid molecule, wherein both elements are contained in the same nucleic acid molecule, and wherein the first element is closer to the 5' end of the nucleic acid molecule than the second element of the nucleic acid molecule. The second element is then said to be "downstream" of the first element of the nucleic acid molecule. An element that is "upstream" from a second element may be synonymously referred to as "5'" from the second element. For double-stranded nucleic acid molecules, indications are given in terms of (+) strands, e.g., "upstream" and "downstream".
According to the present invention, the term "gene" refers to a specific nucleic acid sequence responsible for the production of one or more cellular products and/or for performing one or more intercellular or intracellular functions. More specifically, the term relates to a nucleic acid portion (DNA or RNA) comprising a nucleic acid encoding a specific protein or a functional or structural RNA molecule.
The term "vector" is used herein in its most general sense and includes any intermediate carrier for a nucleic acid, e.g., such that the nucleic acid can be introduced into a prokaryotic and/or eukaryotic host cell and, when appropriate, integrated into the genome. Preferably, such vectors replicate and/or express in cells. Vectors include plasmids, phagemids, viral genomes, and portions thereof.
In the context of the present invention, the term "RNA" relates to a molecule comprising and preferably consisting entirely or essentially of ribonucleotide residues and includes all RNA types described herein. The term "ribonucleotide" relates to a nucleotide having a hydroxyl group at the 2' position of the beta-D-ribofuranosyl group. The term "RNA" includes double-stranded RNA, single-stranded RNA, isolated RNA, e.g., partially or fully purified RNA, substantially pure RNA, synthetic RNA, and recombinantly produced RNA (e.g., modified RNA that differs from naturally occurring RNA by the addition, deletion, substitution, and/or alteration of one or more nucleotides). Such alterations may include, for example, the addition of non-nucleotide material to the end of the RNA or internally, for example, at one or more nucleotides of the RNA. The nucleotides in the RNA molecule may also include non-standard nucleotides, such as non-naturally occurring nucleotides or chemically synthesized nucleotides or deoxynucleotides. These altered RNAs may be referred to as analogs, particularly analogs of naturally occurring RNAs. The RNA used according to the invention may have a known composition, or the composition of the RNA may be partially or completely unknown.
The term "stability" of an RNA relates to the "half-life" of the RNA. "half-life" refers to the time required to eliminate half the activity, amount, or number of molecules. In the context of the present invention, the half-life of an RNA is indicative of the stability of said RNA. The half-life of an RNA can affect the "duration of expression" of the RNA. It is expected that RNAs with long half-lives will be expressed over extended periods of time.
The term "translation efficiency" relates to the amount of translation product provided by an RNA molecule over a specific period of time.
According to the invention, "double-stranded RNA" or "dsRNA" means RNA having two partially or fully complementary strands.
According to the invention, the RNA is preferably a single-stranded RNA (ssRNA). The term "single-stranded RNA" generally refers to an RNA molecule with which no complementary nucleic acid molecule (generally no complementary RNA molecule) is associated. The single-stranded RNA may comprise self-complementary sequences that allow portions of the RNA to fold back and form secondary structural motifs including, but not limited to, base pairs, stems, stem loops, and bulges (bulks). Single-stranded RNA can exist as the negative [ (-) strand ] or as the positive [ (+) strand ]. The (+) strand is a strand that contains or encodes genetic information. The genetic information may be, for example, a polynucleotide sequence encoding a protein. When the (+) strand RNA encodes a protein, the (+) strand may be used directly as a template for translation (protein synthesis). The (-) strand is the complement of the (+) strand. In the case of double-stranded RNA, the plus and minus strands are two separate RNA molecules, and the two RNA molecules associate with each other to form double-stranded RNA ("duplex RNA").
Particularly preferred single-stranded RNAs according to the invention are mRNA and replicon-RNA, e.g.self-replicating RNA. According to the invention, the RNA may be an RNA coding for a peptide or protein. Preferably, the RNA is a pharmaceutically active RNA.
A "pharmaceutically active RNA" is an RNA that encodes a pharmaceutically active peptide or protein (e.g., an antigen or an immunologically active compound (which does not encode an antigen)) or that itself has a pharmaceutical activity, e.g., it has one or more pharmaceutical activities, such as those described for a pharmaceutically active protein.
According to the present invention, the term "RNA encoding a peptide or protein" means an RNA that, if present in a suitable environment (preferably within a cell), can direct the assembly of amino acids during translation to produce a peptide or protein. Preferably, the coding RNA according to the invention is capable of interacting with the cellular translation machinery, allowing translation of the coding RNA to produce a peptide or protein.
According to the invention, the term "mRNA" means "messenger RNA" and relates to a transcript that is usually produced by using a DNA template and encodes a peptide or protein. Generally, an mRNA comprises a5 '-UTR, a protein coding region, a 3' -UTR, and a poly (A) sequence. mRNA can be produced from a DNA template by in vitro transcription. In vitro transcription methods are known to the skilled worker. For example, various in vitro transcription kits are commercially available. According to the present invention, mRNA can be modified by stabilization modification and capping.
The term "untranslated region" or "UTR" refers to a region in a DNA molecule that is transcribed but not translated into an amino acid sequence, or to a corresponding region in an RNA molecule (e.g., an mRNA molecule). Untranslated regions (UTRs) may be present 5 '(upstream) (5' -UTR) and/or 3 '(downstream) (3' -UTR) of the open reading frame.
If present, the 3 ' -UTR is located at the 3 ' end of the gene, downstream of the stop codon of the protein coding region, but the term "3 ' -UTR" preferably does not comprise a poly (A) tail. Thus, the 3' -UTR is located upstream of, e.g., immediately adjacent to, the poly (a) tail, if present. If present, the 5 '-UTR is located at the 5' end of the gene, upstream of the start codon of the protein coding region. The 5 ' -UTR is located downstream of the 5 ' -cap (if present), e.g., directly adjacent to the 5 ' -cap. According to the invention, the 5 '-and/or 3' -untranslated regions may be functionally linked to the open reading frame, so that these regions are associated with the open reading frame in such a way that the stability and/or the translation efficiency of the RNA comprising said open reading frame is increased.
According to the present invention, the term "poly (a) sequence" or "poly (a) tail" refers to an uninterrupted or interrupted sequence of adenylate residues, which is typically located at the 3' end of an RNA molecule. The uninterrupted sequence is characterized by consecutive adenylate residues. In nature, uninterrupted poly (A) sequences are typical. Although poly (a) sequences are not normally encoded in eukaryotic DNA, they are linked to the free 3' end of RNA following transcription by a template-independent RNA polymerase during eukaryotic transcription in the nucleus, and the present invention encompasses poly (a) sequences encoded by DNA.
Terms such as "5 ' -cap", "5 ' -cap structure" or "cap structure" are used synonymously to refer to the dinucleotide found at the 5 ' end of some eukaryotic primary transcripts (e.g., precursor messenger RNAs). A5 ' -cap is a structure in which the (optionally modified) guanosine is bonded to the first nucleotide of the mRNA molecule through a5 ' to 5 ' triphosphate linkage (or modified triphosphate linkage in the case of certain cap analogs). The term may refer to a conventional cap or cap analogue.
The RNA molecule according to the invention may be characterized by a5 ' -cap, a5 ' -UTR, a3 ' -UTR, a poly (a) sequence and/or an adaptation of codon usage.
The RNA molecules used according to the invention preferably have a size of more than 2000 bases, preferably more than 3000 bases, more than 4000 bases, more than 5000 bases, more than 6000 bases, more than 7000 bases, more than 8000 bases, more than 9000 bases, or more than 10000 bases. The size of the RNA molecules used according to the invention is preferably from 6000 to 20000 bases, preferably from 6000 to 15000 bases, preferably from 9000 to 12000 bases.
According to the present invention, the term "expression" is used in its most general sense and includes the production of RNA and/or protein. It also includes partial expression of nucleic acids. Furthermore, expression may be transient or stable. With respect to RNA, the terms "expression" or "translation" relate to the process in the ribosomes of a cell by which a strand of coding RNA (e.g., messenger RNA) directs the assembly of an amino acid sequence to make a peptide or protein.
The term "transcription" relates to the process during which a nucleic acid molecule having a specific nucleic acid sequence ("nucleic acid template") is read by an RNA polymerase such that the RNA polymerase produces a single-stranded RNA molecule. During transcription, genetic information in the nucleic acid template is transcribed. The nucleic acid template may be DNA; however, for example, in the case of transcription from an alphavirus nucleic acid template, the template is typically RNA. Subsequently, the transcribed RNA can be translated into protein. According to the present invention, the term "transcription" includes "in vitro transcription", wherein the term "in vitro transcription" relates to a process in which RNA (in particular mRNA) is synthesized in vitro in a cell-free system. Preferably, cloning vectors are used to generate transcripts. These cloning vectors are generally designated as transcription vectors and are encompassed by the term "vector" according to the present invention. The cloning vector is preferably a plasmid. According to the invention, the RNA is preferably an in vitro transcribed RNA (IVT-RNA) and can be obtained by in vitro transcription of a suitable DNA template. The promoter used to control transcription may be any promoter of any RNA polymerase. DNA templates for in vitro transcription can be obtained by cloning nucleic acids, in particular cDNA, and introducing them into suitable vectors for in vitro transcription. cDNA can be obtained by reverse transcription of RNA.
The single-stranded nucleic acid molecules produced during transcription typically have a nucleic acid sequence that is the complement of the template.
According to the present invention, the term "template" or "nucleic acid template" or "template nucleic acid" generally refers to a nucleic acid sequence that can be replicated or transcribed.
According to the present invention, the term "expression control sequence" includes promoters, ribosome binding sequences and other control elements which control the transcription of genes or the translation of the resulting RNA. In some particular embodiments of the invention, the expression control sequence may be modulated. The precise structure of the expression control sequence may vary depending on the species or cell type, but typically includes 5 ' -nontranscribed sequences and 5 ' -and 3 ' -nontranslated sequences that are involved in initiating transcription and translation, respectively. More specifically, the 5' -non-transcribed expression control sequence comprises a promoter region encompassing a promoter sequence for transcriptional control of a functionally linked gene. Expression control sequences may also include enhancer sequences or upstream activator sequences. Expression control sequences for DNA molecules typically include 5 ' -nontranscribed sequences as well as 5 ' -and 3 ' -untranslated sequences, such as TATA boxes, capping sequences, CAAT sequences, and the like. The expression control sequences of alphavirus RNA can include a subgenomic promoter and/or one or more conserved sequence elements. As described herein, a particular expression control sequence according to the present invention is a subgenomic promoter of alphavirus.
The term "promoter" or "promoter region" refers to a nucleic acid sequence that controls the synthesis of a transcript (e.g., a transcript comprising a coding sequence) by providing a recognition and binding site for RNA polymerase. The promoter region may also contain recognition or binding sites for other factors involved in regulating transcription of the gene. Promoters can control the transcription of prokaryotic or eukaryotic genes. A promoter may be "inducible" and initiate transcription in response to an inducer, or may be "constitutive" if transcription is not controlled by the inducer. If no inducer is present, the inducible promoter is expressed only to a very small extent or not at all. In the presence of an inducer, the gene is "turned on" or the level of transcription is increased. This is usually mediated by the binding of specific transcription factors. As described herein, a specific promoter according to the invention is a subgenomic promoter of alphavirus. Other specific promoters are the genomic plus or minus strand promoters of alphaviruses.
The term "core promoter" refers to a nucleic acid sequence comprised by a promoter. The core promoter is usually the minimal portion of the promoter required to properly initiate transcription. The core promoter typically comprises a transcription initiation site and a binding site for RNA polymerase.
The nucleic acid sequences described herein, in particular the transcribable and encoding nucleic acid sequences, may be combined with any expression control sequence, which may be homologous or heterologous to said nucleic acid sequence, wherein the term "homologous" refers to the fact that the nucleic acid sequence is also naturally functionally linked to the expression control sequence, and the term "heterologous" refers to the fact that the nucleic acid sequence is not naturally functionally linked to the expression control sequence.
If the nucleic acid sequence (in particular a nucleic acid sequence encoding a peptide or protein) and the expression control sequence are covalently linked to one another in such a way that: the transcription or expression of the transcribable and/or coding nucleic acid sequences is controlled or influenced by the expression control sequence and are then "functionally" linked to one another.
According to the present invention, "functional linkage" or "functional connection" relates to a connection within a functional relationship. A nucleic acid is "functionally linked" if it is functionally related to another nucleic acid sequence. For example, a promoter is functionally linked to a coding sequence if it affects the transcription of the coding sequence. Functionally linked nucleic acids are generally adjacent to each other, separated by other nucleic acid sequences when appropriate.
In some particular embodiments, according to the invention, the nucleic acid is functionally linked to an expression control sequence, which may be homologous or heterologous to the nucleic acid.
"polymerase" generally refers to a molecular entity capable of catalyzing the synthesis of a polymer molecule from a monomeric building block. An "RNA polymerase" is a molecular entity capable of catalyzing the synthesis of an RNA molecule from a ribonucleotide building block. A "DNA polymerase" is a molecular entity capable of catalyzing the synthesis of a DNA molecule from a deoxyribonucleotide building block. In the case of DNA polymerases and RNA polymerases, the molecular entity is typically a protein or an assembly or complex of multiple proteins. Generally, a DNA polymerase synthesizes a DNA molecule based on a template nucleic acid, which is typically a DNA molecule. Typically, an RNA polymerase synthesizes an RNA molecule based on a template nucleic acid, which is either a DNA molecule (in which case the RNA polymerase is a DNA-dependent RNA polymerase, DdRP) or an RNA molecule (in which case the RNA polymerase is an RNA-dependent RNA polymerase, RdRP).
An "RNA-dependent RNA polymerase" or "RdRP" is an enzyme that catalyzes the transcription of RNA from an RNA template. In the case of alphavirus RNA-dependent RNA polymerase, sequential synthesis of the negative strand complementary strand of genomic RNA and the (+) strand of genomic RNA results in RNA replication. Thus, the alphavirus RNA-dependent RNA polymerase is synonymously referred to as "RNA replicase". In nature, RNA-dependent RNA polymerases are typically encoded by all RNA viruses except retroviruses. A typical representative of viruses that encode RNA-dependent RNA polymerases is the alphavirus.
According to the present invention, "RNA replication" generally refers to an RNA molecule synthesized based on the nucleotide sequence of a given RNA molecule (template RNA molecule). The synthetic RNA molecule can be, for example, identical to or complementary to the template RNA molecule. Generally, RNA replication can occur through the synthesis of DNA intermediates, or can occur directly through RNA-dependent RNA replication mediated by RNA-dependent RNA polymerase (RdRP). In the case of alphaviruses, RNA replication does not occur through DNA intermediates, but is mediated by RNA-dependent RNA polymerase (RdRP): the template RNA strand (first RNA strand) or a portion thereof is used as a template for the synthesis of a second RNA strand complementary to the first RNA strand or portion thereof. The second RNA strand, or portion thereof, can in turn optionally be used as a template for the synthesis of a third RNA strand that is complementary to the second RNA strand, or portion thereof. Thus, the third RNA strand is identical to the first RNA strand or a portion thereof. Thus, the RNA-dependent RNA polymerase is able to directly synthesize the complementary RNA strand of the template, and is able to indirectly synthesize the same RNA strand (via the complementary intermediate strand).
According to the present invention, the term "template RNA" refers to RNA that can be transcribed or replicated by RNA-dependent RNA polymerase.
In a preferred embodiment of the invention, the RNA used according to the invention is a replicon RNA or simply a "replicon", in particular a self-replicating RNA. In a particularly preferred embodiment, the replicon or self-replicating RNA is derived from, or comprises elements derived from, an ssRNA virus, particularly a positive-stranded ssRNA virus (e.g., alphavirus).
Generally, RNA viruses are a diverse group of infectious particles having an RNA genome. RNA viruses can be subdivided into single stranded RNA (ssRNA) and double stranded RNA (dsRNA) viruses, and ssRNA viruses can generally be further divided into positive-strand [ (+) strand ] and/or negative-strand [ (-) strand ] viruses. Positive-stranded RNA viruses initially appear to be attractive as delivery systems in biomedicine (prima facies) because their RNA can be used directly as a template for translation in host cells.
Alphaviruses are a typical representation of positive-stranded RNA viruses. Hosts for alphaviruses include a wide variety of organisms, including insects, fish, and mammals, such as domestic animals and humans. Alphaviruses replicate in the cytoplasm of infected cells (for reviews of the alphavirus life cycle see Jos et al, Future microbiol, 2009, vol. 4, pages 837-856). The total genomic length of many alphaviruses is typically 11,000 to 12,000 nucleotides, and the genomic RNA typically has a5 '-cap and a 3' poly (a) tail. The genome of alphaviruses encodes non-structural proteins (involved in transcription, modification and replication of viral RNA and protein modification) and structural proteins (forming viral particles). There are usually two Open Reading Frames (ORFs) in the genome. The four nonstructural proteins (nsP1 to nsP4) are typically encoded together by a first ORF starting near the 5 'end of the genome, while the alphavirus structural proteins are encoded together by a second ORF found downstream of the first ORF and extending near the 3' end of the genome. Generally, the first ORF is larger than the second ORF in a ratio of about 2: 1.
In cells infected with alphaviruses, only nucleic acid sequences encoding non-structural proteins are translated from genomic RNA, while genetic information encoding structural proteins is translated from subgenomic transcripts, which are RNA molecules similar to eukaryotic messenger RNA (mRNA; Gould et al, 2010, Antiviral Res., Vol 87, p 111-124). Following infection, i.e., at an early stage in the viral life cycle, the (+) strand genomic RNA acts directly like messenger RNA for translation of the open reading frame encoding the nonstructural polyprotein (nsP 1234). In some alphaviruses, an opal stop codon (opal stop codon) is present between the coding sequences of nsP3 and nsP 4: when translation was terminated at the opal stop codon, polyprotein P123 containing nsP1, nsP2, and nsP3 was produced, and after reading through the opal codon polyprotein P1234 additionally containing nsP4 was produced (Strauss & Strauss, Microbiol. Rev., 1994, Vol 58, pp 491-562; Rupp et al, 2015, J.Gen.virology, Vol 96, pp 2483-2500). nsP1234 was cleaved from proteolysis into fragments nsP123 and
In infected cells, subgenomic RNA as well as new genomic RNA is provided with a 5' -cap by nsP1 (Pettersson et al 1980, Eur.J.biochem.105, 435-443; Rozanov et al 1992, J.Gen.virology, Vol.73, p.2129-2134) and a poly A [ poly (A) ] tail by nsP4 (Rubach et al Virology, 2009, Vol.384, p.201-208). Thus, both subgenomic and genomic RNAs resemble messenger RNA (mRNA).
Alphavirus structural proteins are typically encoded by a single open reading frame under the control of a subgenomic promoter (Strauss & Strauss, microbiol. rev., 1994, volume 58, pages 491-562). Subgenomic promoters are recognized by cis-acting alphavirus nonstructural proteins. In particular, alphavirus replicase synthesizes (+) strand subgenomic transcripts using the negative (-) strand of genomic RNA as a template. The (+) strand subgenomic transcript encodes an alphavirus structural protein (Kim et al, 2004, Virology, Vol. 323, pp. 153-163, Vasiljeva et al, 2003, J.biol.Chem. Vol. 278, pp. 41636-41645). The subgenomic RNA transcript serves as a template for translation of an open reading frame encoding a structural protein that is a polyprotein, and the polyprotein is cleaved to produce the structural protein. In the later stages of alphavirus infection in the host cell, the packaging signal located within the coding sequence of nsP2 ensures selective packaging of genomic RNA into budding virions (budding virus) packaged by structural proteins (White et al, 1998, j.virol., vol 72, pp 4320-4326).
In infected cells, (-) strand RNA synthesis is typically observed only in the first 3 to 4 hours after infection and is not detected at a later stage, when only (+) strand RNA (both genomic and subgenomic) synthesis is observed. According to Frolov et al, 2001, RNA, Vol.7, pp.1638-1651, the main model for regulating RNA synthesis indicates dependence on unstructured polyprotein processing: initial cleavage of the nonstructural polyprotein, nsP1234, yielded nsP123 and
The synthesis of alphavirus RNA is also regulated by cis-acting RNA elements, including four conserved sequence elements (CSEs; Strauss & Strauss, Microbiol. Rev., 1994, Vol.58, pp.491-562; and Frolov, 2001, RNA, Vol.7, pp.1638-1651).
Generally, the 5' replication recognition sequences of alphavirus genomes are characterized by low overall homology between different alphaviruses, but have conserved predicted secondary structures. The 5 'replication recognition sequence of the alphavirus genome is not only involved in translation initiation, but also comprises a 5' replication recognition sequence containing two conserved sequence elements,
In contrast, the 3' end sequence of the alphavirus genome (i.e., the sequence immediately upstream of the poly (A) sequence) is characterized by a conserved primary structure, particularly by conserved sequence element 4(CSE 4), also known as the "19-nt conserved sequence", which is important for the initiation of (-) strand synthesis.
CSE3, also known as a "junction sequence", is a conserved sequence element on the (+) strand of the alphavirus genomic RNA, and the CSE3 complement on the (-) strand serves as a promoter for transcription of subgenomic RNA (Strauss & Strauss, Microbiol. Rev., 1994, Vol.58, pp.491-562; Frolov et al, 2001, RNA, Vol.7, pp.1638-1651). CSE3 typically overlaps with the region encoding the C-terminal fragment of
In addition to alphavirus proteins, host cytokines (which may be proteins) may also bind to conserved sequence elements (Strauss & Strauss, supra).
Alphavirus-derived vectors have been proposed for the delivery of exogenous genetic information into target cells or target organisms. In a simple method, the open reading frame encoding the alphavirus structural protein is replaced with an open reading frame encoding the protein of interest. The alphavirus-based trans-replication system relies on alphavirus nucleotide sequence elements on two separate nucleic acid molecules: one nucleic acid molecule encodes a viral replicase (usually the polyprotein nsP1234) and the other nucleic acid molecule is capable of being replicated in trans by the replicase (hence the name trans-replication system). Trans-replication requires the presence of both nucleic acid molecules in a given host cell. Nucleic acid molecules capable of being replicated in trans by replicase must contain certain alphavirus sequence elements to allow for recognition by the alphavirus replicase and RNA synthesis.
According to the present invention, the term "alphavirus" is to be understood broadly and includes any viral particle having alphavirus characteristics. A characteristic of alphaviruses includes the presence of (+) strand RNA, which encodes genetic information suitable for replication in a host cell, including RNA polymerase activity. Additional features of many alphaviruses are described, for example, in Strauss & Strauss, Microbiol. Rev., 1994, Vol.58, pages 491-562. The term "alphavirus" includes alphaviruses found in nature, as well as any variants or derivatives thereof. In some embodiments, the variant or derivative is not found in nature.
In one embodiment, the alphavirus is one found in nature. Typically, alphaviruses found in nature are infectious to any one or more eukaryotic organisms, such as animals, including vertebrates (e.g., humans) and arthropods (e.g., insects).
The alphavirus found in nature is preferably selected from the following: bama Forest virus complex (including bama Forest virus)); eastern equine encephalitis complex (Eastern equine encephalitis virus) (including seven antigen types of Eastern equine encephalitis virus)); middelberg virus complex (middlelburg viroplex) (including middelberg virus)); andumu virus complex (including andumu virus)); semliki Forest virus complex (Semliki Forest virus complex) (including Bebaru virus), Chikungunya virus (Chikungunya virus), marraya virus (Mayaro virus) and its subtype uravirus (Una virus), ornithoni virus (O' Nyong virus) and its subtype Igbo-Ora virus, Ross River virus (Ross River virus) and its subtype babaru virus (Bebaru virus), geta virus (Getah virus), aigren virus (sagima virus), Semliki Forest virus (Semliki Forest virus) and its subtype Me Tri virus); venezuelan equine encephalitis complex (Venezuelan equine encephalitis virus) (including cabasco virus, marsh virus, mostemdaps paderas virus, mucuna virus, paumar virus, paumana virus, naxsula virus, rujor virus, troaca virus, and subtypes thereof bitavirus (Bijou Bridge virus), Venezuelan equine encephalitis virus (Venezuelan encitisis)); western equine encephalitis complex (Western equine encephalitis complex) (including ola virus (Aura virus), babankan virus (Babanki virus), cumin agagacke virus (kyzygaach virus), Sindbis virus (Sindbis virus), okibu virus (ockbo virus), vataro virus (whataroavir), bogey virus (Buggy Creek virus), morberg virus (Fort Morgan virus), highland J virus (Highlands J virus), Western equine encephalitis virus (Western equene virus)); and some unclassified viruses, including salmon pancreatic disease virus; sleep disorder virus; southern elephant seal virus; tonatevirus (Tonate virus). More preferably, the alphavirus is selected from the following: semliki forest virus complex (including the types of viruses described above, including semliki forest virus), western equine encephalitis complex (including the types of viruses described above, including sindbis virus), eastern equine encephalitis virus (including the types of viruses described above), venezuelan equine encephalitis complex (including the types of viruses described above, including venezuelan equine encephalitis virus).
In another preferred embodiment, the alphavirus is a semliki forest virus. In another alternative preferred embodiment, the alphavirus is sindbis virus. In another alternative preferred embodiment, the alphavirus is venezuelan equine encephalitis virus.
In some embodiments of the invention, the alphavirus is not an alphavirus found in nature. Typically, an alphavirus not found in nature is a variant or derivative of an alphavirus found in nature that is distinguished from an alphavirus found in nature by at least one mutation in the nucleotide sequence (i.e., genomic RNA). Mutations in the nucleotide sequence may be selected from insertions, substitutions or deletions of one or more nucleotides compared to alphaviruses found in nature. Mutations in a nucleotide sequence may or may not be related to mutations in the polypeptide or protein encoded by the nucleotide sequence. For example, an alphavirus not found in nature can be an attenuated alphavirus. An attenuated alphavirus not found in nature is an alphavirus which usually has at least one mutation in its nucleotide sequence by which it is distinguished from an alphavirus found in nature and which is not infectious at all, or infectious but has a lower ability to produce a disease or has no ability to produce a disease at all. As an illustrative example, TC83 is an attenuated alphavirus that is distinguished from Venezuelan Equine Encephalitis Virus (VEEV) found in nature (McKinney et al, 1963, am.J.Trop.Med.Hyg., 1963, Vol.12; pp.597-603).
Members of the alphavirus genus can also be classified based on their relative clinical features in humans: alphaviruses mainly associated with encephalitis, and alphaviruses mainly associated with fever, rash, and polyarthritis.
The term "alphaviral" means found in, or derived from, an alphavirus, e.g., by genetic engineering.
According to the invention, "SFV" stands for Semliki forest virus. According to the invention, "SIN" or "SINV" stands for Sindbis virus. According to the invention, "VEE" or' "VEEV" stands for venezuelan equine encephalitis virus.
According to the present invention, the term "alphavirus" or "derived from an alphavirus" refers to an entity derived from an alphavirus. For example, an alphavirus protein may refer to a protein found in and/or encoded by an alphavirus; the nucleic acid sequence of an alphavirus may refer to a nucleic acid sequence found in and/or encoded by an alphavirus. Preferably, a "nucleic acid sequence of an alphavirus" refers to a nucleic acid sequence of a "genome of an alphavirus" and/or of a "genomic RNA of an alphavirus".
According to the present invention, the term "alphavirus RNA" refers to any one or more of alphavirus genomic RNA (i.e., (+) strand), the complementary strand of alphavirus genomic RNA (i.e., (-) strand, and the subgenomic transcript (i.e., (+) strand), or any fragment thereof.
According to the invention, "alphavirus genome" refers to the genomic (+) strand RNA of an alphavirus.
According to the present invention, the term "native alphavirus sequence" and similar terms generally refer to the (e.g., nucleic acid) sequence of a naturally occurring alphavirus (an alphavirus found in nature). In some embodiments, the term "native alphavirus sequence" also includes sequences of attenuated alphaviruses.
According to the present invention, the term "5 'replication recognition sequence" preferably refers to a contiguous nucleic acid sequence, preferably a ribonucleic acid sequence, which is identical or homologous to a 5' fragment of the alphavirus genome. A "5' replication recognition sequence" is a nucleic acid sequence recognized by an alphavirus replicase. The term 5 ' replication recognition sequence includes both the native 5 ' replication recognition sequence and functional equivalents thereof, such as functional variants of the 5 ' replication recognition sequence of an alphavirus found in nature. The 5' replication recognition sequence is required for the synthesis of the negative-strand complementary strand of the alphavirus genomic RNA and for the synthesis of the (+) strand viral genomic RNA based on the negative-strand template. The natural 5' replication recognition sequence typically encodes at least an N-terminal fragment of
According to the present invention, the term "3 'replication recognition sequence" preferably refers to a contiguous nucleic acid sequence, preferably a ribonucleic acid sequence, which is identical or homologous to a 3' fragment of the alphavirus genome. A "3' replication recognition sequence" is a nucleic acid sequence recognized by an alphavirus replicase. The term 3 ' replication recognition sequence includes both the native 3 ' replication recognition sequence and functional equivalents thereof, such as functional variants of the 3 ' replication recognition sequence of an alphavirus found in nature. The 3' replication recognition sequence is required for the synthesis of the negative strand complementary strand of the alphavirus genomic RNA. In one embodiment, the 3' replication recognition sequence comprises conserved sequence element 4(CSE 4) of the alphavirus genome or a variant thereof and optionally a poly (a) tail of the alphavirus genome.
The term "conserved sequence element" or "CSE" refers to a nucleotide sequence found in alphavirus RNA. These sequence elements are referred to as "conserved" because orthologs are present in the genomes of different alphaviruses, and orthologous CSEs of different alphaviruses preferably have a high percentage of sequence identity and/or similar secondary or tertiary structure. The term CSE includes
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According to the present invention, the term "subgenomic promoter" or "SGP" refers to a nucleic acid sequence upstream (5') of a nucleic acid sequence (e.g. a coding sequence) which controls transcription of said nucleic acid sequence by providing an RNA polymerase (typically an RNA-dependent RNA polymerase, in particular a recognition and binding site for a functional alphavirus nonstructural protein). SGPs may also contain other recognition or binding sites for other factors. Subgenomic promoters are typically genetic elements of positive-stranded RNA viruses (e.g., alphaviruses). A subgenomic promoter of an alphavirus is a nucleic acid sequence contained in the viral genomic RNA. Subgenomic promoters are generally characterized in that they allow transcription (RNA synthesis) to be initiated in the presence of an RNA-dependent RNA polymerase (e.g., a functional alphavirus nonstructural protein). The RNA (-) strand (i.e., the complementary strand of the alphavirus genomic RNA) serves as a template for the synthesis of the (+) strand subgenomic transcript, and synthesis of the (+) strand subgenomic transcript typically begins at or near the subgenomic promoter. The term "subgenomic promoter" as used herein is not limited to any particular location in a nucleic acid comprising such a subgenomic promoter. In some embodiments, the SGP is identical to CSE3 or overlaps with CSE3 or comprises
The term "subgenomic transcript" or "subgenomic RNA" synonymously refers to an RNA molecule that can be obtained by transcription using the RNA molecule as a template ("template RNA"), wherein the template RNA comprises a subgenomic promoter that controls transcription of the subgenomic transcript. Subgenomic transcripts can be obtained in the presence of RNA-dependent RNA polymerase (particularly functional alphavirus non-structural proteins). For example, the term "subgenomic transcript" can refer to an RNA transcript prepared in an alphavirus-infected cell using the negative-strand complementary strand of the alphavirus genomic RNA as a template. However, the term "subgenomic transcript" as used herein is not limited thereto, and also includes transcripts that can be obtained by using a heterologous RNA as a template. For example, subgenomic transcripts can also be obtained by using the negative (-) strand complement of the replicon comprising the SGP according to the present invention as a template. Thus, the term "subgenomic transcript" may refer to an RNA molecule obtainable by transcription of an RNA fragment of an alphavirus genome, as well as an RNA molecule obtainable by transcription of a fragment of a replicon according to the invention.
According to the invention, a nucleic acid construct capable of replication by a replicase, preferably an alphavirus replicase, is referred to as a replicon. According to the present invention, the term "replicon" defines an RNA molecule that can be replicated by an RNA-dependent RNA polymerase, resulting in one or more identical or essentially identical copies of the RNA replicon, without DNA intermediates. By "without a DNA intermediate" is meant that no copies of deoxyribonucleic acid (DNA) of the replicon or complementary sequence are formed during the formation of copies of the RNA replicon and/or that no deoxyribonucleic acid (DNA) molecules are used as template during the formation of copies of the RNA replicon or its complementary sequence. The function of replicase is usually provided by functional alphavirus nonstructural proteins.
According to the present invention, the terms "replicable" and "capable of replication" generally describe that one or more identical or substantially identical copies of a nucleic acid can be prepared. When used with the term "replicase," e.g., in "capable of replication by a replicase," the terms "replicable" and "capable of replication" describe a functional characteristic of a nucleic acid molecule (e.g., an RNA replicon) with respect to the replicase. These functional features include at least one of: (i) the replicase is capable of recognizing a replicon and (ii) the replicase is capable of functioning as an RNA-dependent RNA polymerase (RdRP). Preferably, the replicase is capable of both (i) recognizing the replicon and (ii) acting as an RNA-dependent RNA polymerase.
The expression "capable of recognizing" describes that the replicase is capable of physically associating with the replicon, and preferably, that the replicase is capable of binding to the replicon, typically noncovalently. The term "associated with" may mean that the replicase has the ability to bind to one or more of: conserved sequence element 1(CSE 1) or its complement (if comprised by the replicon), conserved sequence element 2(CSE2) or its complement (if comprised by the replicon), conserved sequence element 3(CSE 3) or its complement (if comprised by the replicon), conserved sequence element 4(CSE 4) or its complement (if comprised by the replicon). Preferably, the replicase is capable of binding to CSE 2[ i.e., to (+) strand ] and/or to CSE 4[ i.e., to (+) strand ], or to the complement of CSE 1 [ i.e., to (-) strand ] and/or to the complement of CSE3 [ i.e., to (-) strand ].
The expression "capable of acting as an RdRP" means that the replicase is capable of catalyzing the synthesis of the complementary strand of the (-) strand of the (+) strand RNA of an alphavirus genome, wherein the (+) strand RNA has the function of a template, and/or the replicase is capable of catalyzing the synthesis of the (+) strand alphavirus genome RNA, wherein the (-) strand RNA has the function of a template. Generally, the expression "capable of functioning as an RdRP" may also include replicase capable of catalyzing the synthesis of a (+) strand subgenomic transcript, wherein the (-) strand RNA has template function, and wherein the synthesis of the (+) strand subgenomic transcript generally begins at the alphavirus subgenomic promoter.
The expressions "capable of binding" and "capable of acting as an RdRP" refer to the ability to function under normal physiological conditions. In particular, they refer to the conditions within a cell that expresses a functional alphavirus nonstructural protein or has been transfected with a nucleic acid encoding a functional alphavirus nonstructural protein. The cell is preferably a eukaryotic cell. The binding capacity and/or the capacity to act as an RdRP can be tested experimentally, for example, in a cell-free in vitro system or in eukaryotic cells. Optionally, the eukaryotic cell is a cell from a species for which the particular alphavirus representing the source of the replicase is infectious. For example, when using an alphavirus replicase from a specific alphavirus that is infectious to humans, the normal physiological conditions are those in human cells. More preferably, the eukaryotic cells (in one example, human cells) are from the same tissue or organ to which the particular alphavirus representing the origin of the replicase is infectious.
According to the invention, "compared to a native alphavirus sequence" and similar terms refer to sequences that are variants of a native alphavirus sequence. The variant is not usually a native alphavirus sequence per se.
In one embodiment, the RNA replicon comprises a replication recognition sequence, such as a5 'replication recognition sequence and a 3' replication recognition sequence. Replication recognition sequences are nucleic acid sequences that can be recognized by functional alphavirus nonstructural proteins. In other words, functional alphavirus nonstructural proteins are able to recognize replication recognition sequences. Preferably, the 5 'replication recognition sequence is located at the 5' end of the replicon. In one embodiment, the 5' replication recognition sequence comprises or consists of
In one embodiment, the 5 'replication recognition sequence and the 3' replication recognition sequence are capable of directing replication of the RNA replicon in the presence of functional alphavirus nonstructural proteins. Thus, when present alone or preferably together, these recognition sequences direct the replication of the RNA replicon in the presence of functional alphavirus nonstructural proteins.
Preferably, the functional alphavirus nonstructural proteins capable of recognizing both the 5 'and 3' replication recognition sequences of the replicon are provided in cis (encoded by the open reading frame on the replicon as the protein of interest) or in trans (encoded by the open reading frame on an independent replicase construct as the protein of interest). In one embodiment, this is achieved when the 5 'and 3' replication recognition sequences are native to the alphavirus from which the functional alphavirus nonstructural proteins are derived. By natural is meant that the natural source of these sequences is the same alphavirus. In an alternative embodiment, the 5 'replication recognition sequence and/or the 3' replication recognition sequence are not native to the alphavirus from which the functional alphavirus nonstructural protein is derived, so long as the functional alphavirus nonstructural protein is capable of recognizing both the 5 'replication recognition sequence and the 3' replication recognition sequence of the replicon. In other words, functional alphavirus nonstructural proteins are compatible with the 5 'replication recognition sequence and the 3' replication recognition sequence. Functional alphavirus nonstructural proteins are considered compatible (cross-viral compatibility) when they are able to recognize the corresponding sequence or sequence element. Any combination of (3 '/5') replication recognition sequences and CSEs, respectively, with non-structural proteins of functional alphaviruses is possible as long as cross-viral compatibility exists. One skilled in the art can readily test cross-viral compatibility by incubating functional alphavirus nonstructural proteins to be tested with RNA having 3 '-and 5' replication recognition sequences to be tested under conditions suitable for RNA replication (e.g., in a suitable host cell). If replication occurs, it is determined that the (3 '/5') replication recognition sequence and the functional alphavirus nonstructural protein are compatible.
In one embodiment of the invention, the replicon is part of a trans-replication system, and thus, the replicon is a trans-replicon. In this embodiment, it is preferred that the RNA replicon does not comprise an open reading frame encoding a functional alphavirus nonstructural protein. Thus, in this embodiment, the invention provides a system comprising two nucleic acid molecules: a first RNA construct for expressing a functional alphavirus non-structural protein (i.e., encoding a functional alphavirus non-structural protein); and a second RNA molecule, an RNA replicon. An RNA construct for expressing a functional alphavirus nonstructural protein is referred to synonymously herein as an "RNA construct for expressing a functional alphavirus nonstructural protein" or a "replicase construct". Functional alphavirus nonstructural proteins are as defined above and are typically encoded by the open reading frame comprised by the replicase construct. The functional alphavirus nonstructural protein encoded by the replicase construct may be any functional alphavirus nonstructural protein capable of replicating a replicon. According to the invention, the replicase constructs may be present with the replicon in the same composition (e.g., as a mixed particle formulation or a combined particle formulation), or in a separate composition (e.g., as separate particle formulations). When the system of the invention is introduced into a cell, preferably a eukaryotic cell, the open reading frame encoding a functional alphavirus nonstructural protein can be translated. After translation, functional alphavirus nonstructural proteins are capable of replicating individual RNA molecules in trans (RNA replicons).
In this context, trans (e.g., in the case of trans-action, trans-regulation) generally means "acting from different molecules" (i.e., intermolecular). This is in contrast to cis (e.g., in the case of cis-acting, cis-regulated), which is generally meant to "act from the same molecule" (i.e., intramolecular). In the case of RNA synthesis, including transcription and RNA replication, the trans-acting element comprises a nucleic acid sequence comprising a gene encoding an enzyme capable of RNA synthesis (RNA polymerase). RNA polymerases use a second nucleic acid molecule (i.e., a nucleic acid molecule other than the nucleic acid molecule encoding the RNA polymerase) as a template for RNA synthesis. Both the RNA polymerase and the nucleic acid sequence comprising the gene encoding the RNA polymerase are said to "trans-act" on the second nucleic acid molecule. In the context of the present invention, the RNA polymerase encoded by the trans-acting RNA may be a functional alphavirus non-structural protein. Functional alphavirus nonstructural proteins are capable of using a second nucleic acid molecule, which is an RNA replicon, as a template for RNA synthesis, including replication of the RNA replicon. An RNA replicon that can replicate by a transreplicase according to the present invention is synonymously referred to herein as a "trans-replicon".
According to the invention, functional alphavirus nonstructural proteins function to amplify the replicon and produce subgenomic transcripts if the subgenomic promoter is present on the replicon. If the replicon encodes a gene of interest for expression, the level of expression and/or the duration of expression of the gene of interest can be trans-regulated by altering the level of functional alphavirus nonstructural proteins.
The trans-replication system of the invention comprises at least two nucleic acid molecules. In a preferred embodiment, the system consists of exactly two RNA molecule replicons and replicase constructs. In some alternative preferred embodiments, the system comprises more than one replicon (each replicon preferably encoding at least one protein of interest), and further comprises a replicase construct. In these embodiments, functional alphavirus nonstructural proteins encoded by the replicase constructs may act on each replicon to drive replication and optionally produce subgenomic transcripts, respectively. For example, each replicon can encode a pharmaceutically active peptide or protein. This is advantageous, for example, if it is desired to vaccinate a subject against several different antigens.
Preferably, the replicase constructs lack Conserved Sequence Elements (CSE) required for (+) strand template-based (-) strand synthesis and/or (-) strand template-based (+) strand synthesis. More preferably, the replicase construct does not contain any alphavirus Conserved Sequence Elements (CSE). In particular, of the four CSEs of the alphavirus (Strauss & Strauss, Microbiol. Rev., 1994, Vol.58, pp.491 to 562; Jos e et al, Future Microbiol., 2009, Vol.4, pp.837 to 856), any one or more of the following CSEs are preferably not present on the replicase construct:
Preferably, the replicase constructs of the invention differ from alphavirus genomic RNA at least in that they are not self-replicating and/or in that they do not comprise an open reading frame under the control of a subgenomic promoter. When self-replication is not possible, the replicase construct may also be referred to as a "suicide construct".
The replicase constructs according to the invention are preferably single stranded RNA molecules. The replicase constructs according to the invention are typically (+) strand RNA molecules. In one embodiment, the replicase constructs of the invention are isolated nucleic acid molecules.
In one embodiment, the RNA (e.g. a replicon according to the invention) comprises at least one open reading frame encoding a peptide or protein of interest. In various embodiments, the peptide or protein of interest is encoded by a heterologous nucleic acid sequence. According to the present invention, the term "heterologous" refers to the fact that a nucleic acid sequence is not naturally linked to a nucleic acid sequence (e.g., an alphavirus nucleic acid sequence) in function or structure.
The RNA according to the invention may encode a single polypeptide or a plurality of polypeptides. Multiple polypeptides may be encoded as a single polypeptide (fusion polypeptide) or as separate polypeptides. In some embodiments, the RNA according to the invention may comprise more than one open reading frame, each of which may or may not be independently selected under the control of a subgenomic promoter in the case of a replicon. Alternatively, the polyprotein or fusion polypeptide comprises separate polypeptides separated by an optional autocatalytic protease cleavage site (e.g., foot and mouth disease virus 2A protein) or intein (intein).
The protein of interest may for example be selected from reporter proteins, pharmaceutically active peptides or proteins, inhibitors of intracellular Interferon (IFN) signalling and functional alphavirus non-structural proteins.
According to the present invention, the term "peptide" includes oligopeptides and polypeptides and refers to substances comprising two or more, preferably 3 or more, preferably 4 or more, preferably 6 or more, preferably 8 or more, preferably 10 or more, preferably 13 or more, preferably 16 or more, preferably 20 or more, and up to preferably 50, preferably 100 or preferably 150 consecutive amino acids linked to each other by peptide bonds. The term "protein" refers to a large peptide, preferably a peptide having at least 151 amino acids, but the terms "peptide" and "protein" are generally used herein as synonyms.
According to the present invention, the terms "peptide" and "protein" include substances containing not only amino acid components but also non-amino acid components (e.g., sugar and phosphate structures), and also include substances containing bonds such as ester, thioether, or disulfide bonds.
According to the invention, the term "variant" with respect to, for example, nucleic acid and amino acid sequences includes any variant, in particular mutants, strain variants, splice variants, conformations, isoforms, allelic variants, species variants and species homologues, in particular those occurring in nature. Allelic variants involve alterations in the normal sequence of a gene, the meaning of which is often unclear. Whole-gene sequencing typically determines many allelic variants of a given gene. With respect to nucleic acid molecules, the term "variant" encompasses degenerate nucleic acid sequences, wherein a degenerate nucleic acid according to the present invention is a nucleic acid that differs from a reference nucleic acid in codon sequence due to the degeneracy of the genetic code (e.g., due to adaptations of codon usage). Species homologs are nucleic acid or amino acid sequences of a species of origin that differ from a given nucleic acid or amino acid sequence. A viral homolog is a nucleic acid or amino acid sequence of a virus of origin that differs from a given nucleic acid or amino acid sequence.
According to the invention, a nucleic acid variant comprises a single or multiple nucleotide deletion, addition, mutation, substitution and/or insertion compared to a reference nucleic acid. Deletions include the removal of one or more nucleotides from the reference nucleic acid. The additive variants comprise 5 '-and/or 3' -terminal fusions of one or more nucleotides (e.g., 1, 2, 3, 5, 10, 20, 30, 50 or more nucleotides). In the case of substitutions, at least one nucleotide in the sequence is removed and at least one other nucleotide is inserted in its place (e.g., transversions and transitions). Mutations include abasic sites, cross-linking sites, and chemically altered or modified bases. The insertion includes adding at least one nucleotide to the reference nucleic acid.
According to the present invention, "nucleotide change" may refer to a single or multiple nucleotide deletion, addition, mutation, substitution and/or insertion compared to a reference nucleic acid. In some embodiments, a "nucleotide change" as compared to a reference nucleic acid is selected from the group consisting of a deletion of a single nucleotide, an addition of a single nucleotide, a mutation of a single nucleotide, a substitution of a single nucleotide, and/or an insertion of a single nucleotide. According to the invention, a nucleic acid variant may comprise one or more nucleotide changes compared to a reference nucleic acid.
Variants of a particular nucleic acid sequence preferably have at least one functional property of the particular sequence and are preferably functionally equivalent to the particular sequence, e.g., a nucleic acid sequence that exhibits the same or similar properties as the particular nucleic acid sequence.
Preferably, the degree of identity between a given nucleic acid sequence and a nucleic acid sequence that is a variant of said given nucleic acid sequence will be at least 70%, preferably at least 75%, preferably at least 80%, more preferably at least 85%, even more preferably at least 90%, or most preferably at least 95%, 96%, 97%, 98% or 99%. Preferably, a degree of identity is given to a region of at least about 30, at least about 50, at least about 70, at least about 90, at least about 100, at least about 150, at least about 200, at least about 250, at least about 300, or at least about 400 nucleotides. In some preferred embodiments, the degree of identity is given over the entire length of the reference nucleic acid sequence.
"sequence similarity" refers to the percentage of amino acids that are identical or represent conservative amino acid substitutions. "sequence identity" between two polypeptide or nucleic acid sequences refers to the percentage of amino acids or nucleotides that are identical between the sequences.
The term "% identity" is particularly intended to mean the percentage of identical nucleotides at the time of optimal alignment between two sequences to be compared, said percentage being purely statistical, and the differences between the two sequences may be randomly distributed over the entire length of the sequences, and the sequences to be compared may comprise additions or deletions compared to the reference sequence in order to obtain optimal alignment between the two sequences. Comparison of two sequences is typically performed by comparing the sequences after optimal alignment with respect to a segment or "comparison window" to determine local regions of the respective sequences. The optimal alignment for comparison can be done manually or by means of the local homology algorithm of Smith and Waterman, 1981, Ads app. math.2, 482, by means of the local homology algorithm of Needleman and Wunsch, 1970, j.mol.biol.48, 443, and by means of the similarity search algorithm of Pearson and Lipman, 1988, proc.natl acad.sci.usa 85, 2444 or by means of Computer programs using said algorithms (GAP, BESTFIT, FASTA, BLAST P, BLAST N and TFASTA in the Wisconsin Genetics software package, Genetics Computer Group, 575Science Drive, Madison, Wis.).
Percent identity is obtained by determining the number of identical positions in the corresponding compared sequences, dividing this number by the number of compared positions and multiplying the result by 100.
For example, the BLAST program "
A nucleic acid is "capable of hybridizing" or "hybridizing" to another nucleic acid if the two sequences are complementary to each other. A nucleic acid is "complementary" to another nucleic acid if the two sequences are capable of forming a stable duplex with each other. According to the present invention, hybridization is preferably carried out under conditions (stringent conditions) that allow specific hybridization between polynucleotides. Stringent conditions are described, for example, in molecular cloning: a Laboratory Manual, J.Sambrook et al, eds., 2 nd edition, Cold Spring Harbor Laboratory press, Cold Spring Harbor, New York, 1989 or Current Protocols in molecular Biology, F.M.Ausubel et al, eds., John Wiley&Sons, inc., New York and means, for example, at 65 ℃ in hybridization buffer (3.5 × SSC, 0.02% Ficoll, 0.02% polyvinylpyrrolidone, 0.02% bovine serum albumin, 2.5mM NaH2PO4(pH 7), 0.5% SDS, 2mM EDTA) SSC of 0.15M sodium chloride/0.15M sodium citrate,
Percent complementarity refers to the percentage of contiguous residues (e.g., 5, 6, 7, 8, 9, 10 out of 10 are 50%, 60%, 70%, 80%, 90%, and 100% complementary) in a nucleic acid molecule that can form hydrogen bonds (e.g., Watson-Crick base pairing) with a second nucleic acid sequence. By "perfectly complementary" or "fully complementary" is meant that all consecutive residues of a nucleic acid sequence will hydrogen bond to the same number of consecutive residues in a second nucleic acid sequence. Preferably, the degree of complementarity according to the invention is at least 70%, preferably at least 75%, preferably at least 80%, more preferably at least 85%, even more preferably at least 90% or most preferably at least 95%, 96%, 97%, 98% or 99%. Most preferably, the degree of complementarity according to the invention is 100%.
The term "derivative" includes any chemical derivatization of nucleic acids at the nucleotide bases, sugars or phosphates. The term "derivative" also includes nucleic acids containing nucleotides and nucleotide analogs that do not naturally occur. Preferably, derivatization of the nucleic acid increases its stability.
According to the invention, "nucleic acid sequence derived from a nucleic acid sequence" refers to a nucleic acid which may be a variant of the nucleic acid from which it is derived.
In one embodiment, the open reading frame encodes a reporter protein. In this embodiment, the open reading frame comprises a reporter gene. Certain genes may be selected as reporters because they confer a characteristic that can be readily determined and measured by the cell or organism in which they are expressed, or because they are selectable markers. Reporter genes are often used as indicators of whether a gene is taken up or expressed by a cell or a population of organisms. Preferably, the expression product of the reporter gene is visually detectable. Common visually detectable reporter proteins typically have fluorescent or luminescent proteins. Examples of specific reporter genes include the gene encoding jellyfish Green Fluorescent Protein (GFP), which causes cells expressing it to emit green light under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to generate light, and Red Fluorescent Protein (RFP). Variants of any of these particular reporter genes are possible, provided that the variant has a visually detectable property. For example, eGFP is a point mutant variant of GFP.
According to the present invention, in one embodiment, the RNA comprises or consists of a pharmaceutically active RNA. A "pharmaceutically active RNA" may be an RNA that encodes a pharmaceutically active peptide or protein. Preferably, the RNA according to the invention encodes a pharmaceutically active peptide or protein. Preferably, the open reading frame encodes a pharmaceutically active peptide or protein. Preferably, the RNA comprises an open reading frame encoding a pharmaceutically active peptide or protein, optionally under the control of a subgenomic promoter in the case of an RNA replicon.
A "pharmaceutically active peptide or protein" has a positive or beneficial effect on a disorder or disease state in a subject when administered to the subject in a therapeutically effective amount. Preferably, the pharmaceutically active peptide or protein has therapeutic or palliative properties and can be administered to ameliorate, reduce, alleviate, reverse one or more symptoms of a disease or disorder, delay its onset, or reduce its severity. Pharmaceutically active peptides or proteins may have prophylactic properties and may be used to delay the onset of a disease or to reduce the severity of such a disease or pathological condition. The term "pharmaceutically active peptide or protein" includes whole proteins or polypeptides, and may also refer to pharmaceutically active fragments thereof. It may also include pharmaceutically active analogs of peptides or proteins. The term "pharmaceutically active peptide or protein" includes peptides and proteins that are antigens, i.e., that elicit an immune response in a subject, which may be therapeutic or partially or fully protective.
In one embodiment, the pharmaceutically active peptide or protein is or comprises an immunologically active compound or antigen or epitope.
According to the present invention, the term "immunologically active compound" relates to any compound that alters the immune response, preferably by inducing and/or inhibiting the maturation of immune cells, inducing and/or inhibiting cytokine biosynthesis, and/or by stimulating antibody production by B cells. In one embodiment, the immune response involves stimulating an antibody response (typically including immunoglobulin G (IgG)) and/or a cellular response (e.g., a T cell response). The immunologically active compounds can have potent immunostimulatory activity, including but not limited to antiviral and antitumor activity, and can also down-regulate other aspects of the immune response, such as shifting the immune response away from TH2Immune response, which can be used to treat a wide variety of TH2A mediated disease.
According to the present invention, the term "antigen" or "immunogen" covers any substance that will elicit an immune response. In particular, "antigen" relates to any substance that reacts specifically with an antibody or T lymphocytes (T cells). According to the present invention, the term "antigen" includes any molecule comprising at least one epitope. Preferably, an antigen in the context of the present invention is a molecule that induces an immune response, optionally after processing, which is preferably specific for the antigen. According to the present invention, any suitable antigen that is a candidate for an immune response, which may be both a humoral immune response and a cellular immune response, may be used. In the case of some embodiments of the invention, the antigen is preferably presented by a cell (preferably by an antigen presenting cell in the case of an MHC molecule), which results in an immune response against the antigen. The antigen is preferably a product corresponding to or derived from a naturally occurring antigen. Such naturally occurring antigens may include or may be derived from allergens, viruses, bacteria, fungi, parasites and other infectious agents and pathogens, or the antigen may also be a tumor antigen. According to the invention, the antigen may correspond to a naturally occurring product, such as a viral protein or a portion thereof. In some preferred embodiments, the antigen is a surface polypeptide, i.e. a polypeptide that is naturally displayed on the surface of a cell, pathogen, bacterium, virus, fungus, parasite, allergen or tumor. The antigen may elicit an immune response against a cell, pathogen, bacteria, virus, fungus, parasite, allergen or tumor.
The term "disease-associated antigen" is used in its broadest sense to refer to any antigen associated with a disease. A disease-associated antigen is a molecule that comprises epitopes that will stimulate the immune system of the host to produce a cellular antigen-specific immune response and/or a humoral antibody response against the disease. Thus, the disease-associated antigen can be used for therapeutic purposes. The disease-associated antigen is preferably associated with a microbial infection (typically a microbial antigen) or with a cancer (typically a tumour).
The term "pathogen" refers to a pathogenic biological substance capable of causing disease in an organism, preferably a vertebrate organism. Pathogens include microorganisms, such as bacteria, unicellular eukaryotes (protozoa), fungi, and viruses.
The terms "epitope", "antigenic peptide", "antigenic epitope", "immunogenic peptide" and "MHC-binding peptide" are used interchangeably herein and refer to an antigenic determinant in a molecule (e.g., an antigen), i.e., to a portion or fragment of an immunologically active compound that is recognized by the immune system (e.g., by T cells), particularly when presented in the context of MHC molecules. The epitope of a protein preferably comprises a continuous or discontinuous portion of the protein and is preferably from 5 to 100, preferably from 5 to 50, more preferably from 8 to 30, most preferably from 10 to 25 amino acids in length, for example the epitope may preferably be 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 amino acids in length. According to the present invention, an epitope can bind to an MHC molecule (e.g., an MHC molecule on the surface of a cell) and thus can be an "MHC-binding peptide" or an "antigenic peptide". The term "major histocompatibility complex" and the abbreviation "MHC" include both MHC class I and MHC class II molecules and refer to the gene complex present in all vertebrates. MHC proteins or molecules are important for signaling between lymphocytes and antigen presenting cells or diseased cells in an immune response, where they bind and present peptides for recognition by T cell receptors. Proteins encoded by MHC are expressed on the cell surface and display self-antigens (peptide fragments from the cell itself) and non-self-antigens (e.g., fragments of invading microorganisms) to T cells. Preferably such immunogenic portions bind to MHC class I or class II molecules. As used herein, an immunogenic portion is said to "bind" to an MHC class I or class II molecule if such binding is detectable using any assay known in the art. The term "MHC-binding peptide" relates to a peptide that binds to an MHC class I and/or MHC class II molecule. In the case of MHC class I/peptide complexes, the binding peptides are typically 8 to 10 amino acids long, although longer or shorter peptides may be effective. In the case of MHC class II/peptide complexes, the binding peptides are typically 10 to 25 amino acids long, and in particular 13 to 18 amino acids long, although longer and shorter peptides may be effective.
In one embodiment, the protein of interest according to the invention comprises epitopes suitable for vaccination of a target organism. The skilled person will know that one of the principles of immunobiology and vaccination is based on the following facts: an immunoprotective response against a disease is generated by immunizing an organism with an antigen that is immunologically relevant to the disease to be treated. According to the invention, the antigen is selected from the group consisting of autoantigens and non-autoantigens. The non-self antigen is preferably a bacterial antigen, a viral antigen, a fungal antigen, an allergen or a parasitic antigen. Preferably, the antigen comprises an epitope capable of eliciting an immune response in the target organism. For example, the epitope may elicit an immune response against a bacterium, virus, fungus, parasite, allergen, or tumor.
In some embodiments, the non-self antigen is a bacterial antigen. In some embodiments, the antigen elicits an immune response against bacteria that infect animals, including birds, fish, and mammals, including domesticated animals. Preferably, the bacteria against which an immune response is elicited are pathogenic bacteria.
In some embodiments, the non-self antigen is a viral antigen. The viral antigen may, for example, be a protein, polypeptide or peptide from a viral surface protein (e.g., a membrane-bound glycoprotein, capsid protein or polypeptide or spike protein or polypeptide). In some embodiments, the antigen elicits an immune response against a virus that infects animals, including birds, fish, and mammals, including domesticated animals. Preferably, the virus against which the immune response is elicited is a pathogenic virus.
In some embodiments, the non-self antigen is a polypeptide or protein from a fungus. In some embodiments, the antigen elicits an immune response against a fungus that infects animals, including birds, fish, and mammals, including domesticated animals. Preferably, the fungus against which the immune response is elicited is a pathogenic fungus.
In some embodiments, the non-self antigen is a polypeptide or protein from a unicellular eukaryotic parasite. In some embodiments, the antigen elicits an immune response against a unicellular eukaryotic parasite (preferably a pathogenic unicellular eukaryotic parasite). Pathogenic unicellular eukaryotic parasites may for example be from Plasmodium (Plasmodium), such as Plasmodium falciparum (p.falciparum), Plasmodium vivax (p.vivax), Plasmodium malariae (p.malariae) or Plasmodium ovale (p.ovale); from the genus Leishmania (Leishmania); or from the genus Trypanosoma (Trypanosoma), such as Trypanosoma cruzi (t.
In some embodiments, the non-self antigen is an allergenic polypeptide or an allergenic protein. The allergenic proteins or allergenic polypeptides are suitable for allergen immunotherapy, also known as hypo-sensitization.
In some embodiments, the antigen is an autoantigen, particularly a tumor antigen. Tumor antigens and their determination are known to those skilled in the art.
In the context of the present invention, the term "tumor antigen" or "tumor-associated antigen" relates to a protein that is specifically expressed under normal conditions in a limited number of tissues and/or organs or in a specific developmental stage, e.g. a tumor antigen may be specifically expressed under normal conditions in gastric tissue (preferably in gastric mucosa), in reproductive organs (e.g. in testis), in trophoblast tissue (e.g. in placenta), or in germ-line cells, and expressed or aberrantly expressed in one or more tumor or cancer tissues. In this case, "limited number" preferably means not more than 3, more preferably not more than 2. In the context of the present invention, tumor antigens include, for example, differentiation antigens, preferably cell type-specific differentiation antigens, i.e., proteins that are specifically expressed in a particular cell type at a particular differentiation stage under normal conditions; cancer/testis antigens, i.e., proteins that are specifically expressed in the testis and sometimes in the placenta under normal conditions; and germline specific antigens. In the context of the present invention, the tumor antigen is preferably associated with the cell surface of cancer cells and is preferably not or only rarely expressed in normal tissues. Preferably, the tumor antigen or abnormal expression of the tumor antigen is indicative of a cancer cell. In the context of the present invention, the tumor antigen expressed by a cancer cell in a subject (e.g. a patient suffering from a cancer disease) is preferably a self-protein in said subject. In some preferred embodiments, the tumor antigen in the context of the present invention is specifically expressed under normal conditions in an unnecessary tissue or organ (i.e., a tissue or organ that does not cause death of the subject when damaged by the immune system), or in a body organ or structure that is inaccessible or nearly inaccessible to the immune system. Preferably, the amino acid sequence of the tumor antigen is identical between the tumor antigen expressed in normal tissue and the tumor antigen expressed in cancer tissue.
Some examples of tumor antigens useful in the present invention are p53, ART-4, BAGE, β -catenin/m, Bcr-ablCAEL, CAP-1, CASP-8, CDC27/m, CDK4/m, CEA, cell surface proteins of the sealin family (e.g. CLAUDIN-6, CLAUDIN-18.2 and CLAUDIN-12), c-MYC, CT, Cyp-B, DAM, ELF2M, ETV6-AML1, G250, GAGE, GnT-V, Gap, HAGE, HER-2/neu, HPV-E7, HPV-E6, HAST-2, hTERT (or hTRT), HPV, LDL, FUT, MAGE-A (preferably MAGE-A1, MAGE-A2, MAGE-A3, MAGE-A4, MAGE-356, MAGE-6, MAGE-A4642, MAGE-A-3642, MAGE-A-364642, MAGE-7, MAGE-A-7, MAGE-11, MAGE-A-364629, MAGE-2, MAGE-A-2, MAGE, MAGE-B, MAGE-C, MART-1/Melan-A, MC1R, myosin/m, MUC1, MUM-1, MUM-2, MUM-3, NA88-A, NF1, NY-ESO-1, NY-BR-1, p190 small BCR-abL, Pm1/RARa, PRAME, protease 3, PSA, PSM, RAGE, RU1 or RU2, SAGE, SART-1 or SART-3, SCGB3A2, SCP1, SCP2, SCP3, SSX, SURVIN, TEL/AML1, TPI/m, TRP-1, TRP-2/2, TPTE and WT. Particularly preferred tumor antigens include CLAUDIN-18.2(CLDN18.2) and CLAUDIN-6(CLDN 6).
It was observed according to the present invention that an effective immune response can be elicited by immunization with PEI formulated self-replicating (self-amplifying) RNA for delivery of antigen. PEI formulated self-replicating RNA derived from Venezuelan Equine Encephalitis Virus (VEEV) is particularly effective for delivering antigens that are membrane proteins.
According to the present invention, the term "membrane protein" relates to a protein associated or bound to a cell membrane. They include integral membrane proteins that are permanently anchored or partially membrane and peripheral membrane proteins that are only temporarily attached to the lipid bilayer or other integral proteins. Integral membrane proteins are divided into transmembrane proteins spanning the membrane and integral monotopic proteins attached to only one side of the membrane. For example, membrane proteins can be divided into a number of general types:
1) type I membrane protein: these proteins have a single transmembrane domain in the mature protein. The N-terminus is extracellular and the C-terminus is cytoplasmic. The N-terminus of the protein characteristically has a classical signal peptide sequence that directs the protein to the ER. Proteins are subdivided into type Ia (comprising a cleavable signal sequence) and type Ib (not comprising a cleavable signal sequence). Examples of type I membrane proteins include, but are not limited to: influenza HA, insulin receptor, glycophorin, LDL receptor and viral G protein.
2) Type II membrane protein: for these single membrane domain proteins, the C-terminus is extracellular and the N-terminus is cytoplasmic. The N-terminus may have a signal anchor sequence. Examples of such protein types include, but are not limited to: influenza neuraminidase, golgi galactosyltransferase, golgi sialyltransferase, sucrase-isomaltase precursor, asialoglycoprotein receptor and transferrin receptor.
3) Multichannel transmembrane protein: in type I and type II membrane proteins, the polypeptide crosses the lipid bilayer once, whereas in multichannel membrane proteins, the polypeptide crosses the membrane multiple times. Multichannel transmembrane proteins are also subdivided into types IIIa and IIIb. The IIIa-type protein has a cleavable signal sequence. The amino terminus of the IIIb-type protein is exposed on the outer surface of the membrane, but there is no cleavable signal sequence. Type IIIa proteins include, but are not limited to, M and L peptides of the photoreactive center. Type IIIb proteins include, but are not limited to, cytochrome P450 and leader peptidase of e. Further examples of multi-channel transmembrane proteins are membrane transporters, such as sugar transporters (glucose, xylose) and ion transporters.
4) Lipid chain anchored membrane protein: these proteins are associated with the membrane bilayer by one or more covalently linked fatty acid chains or other types of lipid chains known as isoprenyls.
5) GPI-anchored membrane protein: these proteins are bound to the membrane via a Glycosylphosphatidylinositol (GPI) anchor.
6) Peripheral membrane protein: these proteins bind indirectly to the membrane through non-covalent interactions with other membrane proteins.
The term "membrane protein" as used herein includes cell membrane proteins of human or non-human cells as well as viral envelope proteins. One embodiment of a membrane protein is influenza Hemagglutinin (HA), which is a glycoprotein found on the surface of influenza viruses. It is responsible for binding the virus to cells with sialic acid on the membrane (e.g. cells in the upper respiratory tract or erythrocytes). After lowering the pH, it is also responsible for fusion of the viral envelope with the endosomal membrane. Other influenza membrane proteins are the M2 protein, which is abundantly expressed on the cell surface, and Neuraminidase (NA).
Accordingly, in one aspect, the present invention relates to a pharmaceutical composition comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkyleneimine.
In another aspect, the present invention relates to a composition for use as a medicament, comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkyleneimine.
In one embodiment of all aspects of the invention, the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the single-stranded RNA (N: P ratio) is from 1.0 to 30, preferably from 2.0 to 15.0, more preferably from 6.0 to 12.0.
In another aspect, the present invention relates to a composition comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the molar ratio of the number of nitrogen atoms (N) in the polyalkyleneimine to the number of phosphorus atoms (P) in the single-stranded RNA (N: P ratio) is 1.0 to 30.0, preferably 2.0 to 15.0, more preferably 6.0 to 12.0.
In one embodiment of all aspects of the invention, the ionic strength of the composition is 50mM or less, preferably wherein the concentration of positively charged monovalent ions is 25mM or less and the concentration of free positively charged divalent cations is 20. mu.M or less.
In another aspect, the present invention relates to a composition comprising:
(a) a single-stranded self-replicating RNA encoding a peptide or protein comprising an antigen or epitope; and
(b) a polyalkylene-imine as a curing agent for a thermoplastic resin,
wherein the ionic strength is 50mM or less.
In one embodiment, the concentration of the positively charged monovalent ion is 25mM or less and the concentration of the positively charged divalent cation is 20. mu.M or less.
In one embodiment of all aspects of the invention, the single stranded self-replicating RNA is a cis replicon.
In one embodiment, the single-stranded self-replicating RNA is derived from Venezuelan Equine Encephalitis Virus (VEEV). In one embodiment, the single-stranded self-replicating RNA corresponds to or substantially corresponds to a genomic RNA of VEEV or an attenuated form thereof, wherein the open reading frame encoding the structural protein is replaced by an open reading frame encoding a peptide or protein comprising an antigen or epitope. In one embodiment, the antigen or peptide or protein comprising the antigen or epitope is a membrane protein, such as a viral envelope protein. In one embodiment, the antigen is influenza hemagglutinin.
In one embodiment, the single-stranded self-replicating RNA is derived from Semliki Forest Virus (SFV). In one embodiment, the single-stranded self-replicating RNA corresponds to or substantially corresponds to a genomic RNA of SFV or an attenuated form thereof, wherein the open reading frame encoding the structural protein is replaced by an open reading frame encoding a peptide or protein comprising an antigen or epitope. In one embodiment, the antigen or peptide or protein comprising the antigen or epitope is not a membrane protein. In one embodiment, the antigen is a viral capsid protein.
In one embodiment of all aspects of the invention, the composition is for intramuscular administration, e.g. by intramuscular injection.
In one embodiment of all aspects of the invention, the single stranded RNA and the polyalkyleneimine are present in a polymeric complex particle.
In one embodiment of all aspects of the invention, the polyalkyleneimine comprises the following general formula (I):
wherein
R is H, acyl or a group comprising the following general formula (II):
wherein R is1Is H or a group comprising the following general formula (III):
n, m and 1 are independently selected from integers from 2 to 10; and is
p, q and r are integers, wherein the sum of p, q and r is such that the average molecular weight of the polymer is 1.5-102To 107Da, preferably 5000 to 105Da, more preferably 10000 to 40000Da, more preferably 15000 to 30000Da, even more preferably 20000 to 25000 Da.
In one embodiment, n, m and 1 are independently selected from 2, 3, 4 and 5, preferably from 2 and 3. In one embodiment, R1Is H. In one embodiment, R is H or acyl.
In one embodiment of all aspects of the invention, the polyalkyleneimine comprises a polyethyleneimine and/or a polypropyleneimine, preferably a polyethyleneimine.
In one embodiment of all aspects of the invention, at least 92% of the N atoms in the polyalkyleneimine are protonatable.
In one embodiment of all aspects of the invention, the composition of the invention comprises one or more additives. In one embodiment, the one or more additives are selected from the group consisting of buffering substances, sugars, stabilizers, cryoprotectants, lyoprotectants, and chelating agents. In one embodiment of all aspects of the invention, the composition of the invention comprises one or more polymers. In one embodiment, the buffer substance comprises at least one selected from the group consisting of: 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid (HEPES), 2- (N-morpholino) ethanesulfonic acid (MES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), acetic acid buffer systems and the like, phosphoric acid buffer systems, or citric acid buffer systems. In one embodiment of all aspects of the invention, the composition of the invention comprises a buffering agent for buffering in the pH range of 4 to 6.5, 3 to 5, or 3, 5 to 4.5. Examples of such buffer systems are acetate buffers or HEPES buffers or phosphate buffers or acetate buffers. In one embodiment, the saccharide comprises at least one selected from the group consisting of: monosaccharides, disaccharides, trisaccharides, oligosaccharides and polysaccharides, preferably selected from glucose, trehalose, sucrose and dextran. In one embodiment, the additive is dextran having an average molar mass of 1kDa to 100 kDa. In one embodiment, the cryoprotectant comprises at least one selected from the group consisting of: glycols, such as ethylene glycol, propylene glycol, and glycerol. In one embodiment, the chelating agent comprises EDTA. In one embodiment, the compositions of the present invention comprise one or more block copolymers comprising ethylene oxide and propylene oxide building blocks. In one embodiment, the composition of the invention comprises a copolymer containing ethylenediamine groups. In one embodiment, the composition of the invention comprises an amphiphilic block copolymer, preferably comprising ethylene oxide and propylene oxide building blocks, optionally further comprising ethylenediamine groups.
In one embodiment of all aspects of the invention, the composition comprises HEPES buffered glucose (HBG or hbgx 1), MES buffered glucose (MBG or mbgx 1), acetate buffered glucose, or HEPES buffered trehalose (HBT or HBT x 1). In one embodiment of all aspects of the invention, the composition comprises glucose or trehalose or sucrose in an acetate buffer at a concentration of 0.1mM to 10 mM. In one embodiment of all aspects of the invention, the composition comprises glucose or trehalose or sucrose in a phosphate buffer at a concentration of 0.1mM to 10 mM.
In one embodiment of all aspects of the invention, the z-average size of the particles is less than 200nm, preferably less than 150nm, and more preferably less than 100 nm. In one embodiment, the z-average size of the particles is from 50nm to 200 nm. In one embodiment of all aspects of the invention, the zeta potential of the particles is 20mV or higher, preferably 25 to 40 mV. In one embodiment of all aspects of the invention, the particles have an electrophoretic mobility (μ) of 1 to 1.6 μm cm/V S. In one embodiment of all aspects of the invention, the z-average size and/or zeta potential and/or electrophoretic mobility of the particles is determined in a suspension comprising the polymer composite particles and HEPES-buffered glucose (HBG) or HEPES-buffered trehalose (HBT). In one embodiment, the HBG comprises 5% glucose (w/v) and 10mM HEPES, pH 7.1, or the HBT comprises 10% trehalose (w/v) and 10mM HEPES, pH 7.1. In one embodiment, the z-average size of the particles is determined by dynamic light scattering and data analysis by an accumulative quantification algorithm. In one embodiment, the migration diffusion coefficient is measured by dynamic light scattering. Then, the Stock-Einstein equation was used to calculate the Z-average. In one embodiment, electrophoretic mobility is measured by laser-doppler electrophoresis. Then, the Henry equation or Smoluchowski equation was used to calculate the zeta potential.
In one embodiment, MBG comprises 5% glucose (w/v) and 10mM MES. In one embodiment, the acetate buffered glucose comprises 5% glucose (w/v) and 10mM acetate.
In one embodiment of all aspects of the invention, the particles are neutral or positively charged, preferably at physiological pH or at a pH of 4.5 to 7.5.
In one embodiment of all aspects of the invention, the single stranded RNA is a molecule of 6000 to 15000 bases, preferably 9000 to 12000 bases.
In one embodiment of all aspects of the invention, the compositions described herein are used in therapy. In one embodiment of all aspects of the invention, the composition described herein is a vaccine composition.
In another aspect, the invention relates to a composition as described herein for use in inducing an immune response. In one embodiment, the composition is administered by intramuscular administration. In one embodiment, the immune response is directed against an antigen or epitope.
In another aspect, the invention relates to a method of inducing an immune response comprising the step of administering a composition described herein. In one embodiment, the composition is administered by intramuscular administration. In one embodiment, the immune response is directed against an antigen or epitope.
In some embodiments, it is not required that the pharmaceutically active peptide or protein be an antigen that elicits an immune response. Suitable pharmaceutically active proteins or peptides may be selected from cytokines and immune system proteins, such as immunologically active compounds (e.g., interleukins, Colony Stimulating Factor (CSF), granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor (GM-CSF), erythropoietin, Tumor Necrosis Factor (TNF), interferons, integrins, addressins, selectins, homing receptors, T cell receptors, immunoglobulins), hormones (insulin, thyroid hormone, catecholamines, gonadotropins, prolactin, oxytocin, dopamine, bovine somatotropin, leptin, etc.), growth hormones (e.g., human growth hormone), growth factors (e.g., epidermal growth factor, nerve growth factor, insulin-like growth factor, etc.), growth factor receptors, enzymes (tissue plasminogen activator, insulin-like growth factor, etc.), enzymes, Streptokinase, cholesterol biosynthesis or degradation, steroid hormone producing enzymes (sterodogenic enzymes), kinases, phosphodiesterases, methylases, demethylases, dehydrogenases, cellulases, proteases, lipases, phospholipases, aromatases, cytochromes, adenylate or guanylate cyclases, ceramidases, etc.), receptors (steroid hormone receptors, peptide receptors), binding proteins (growth hormone or growth factor binding proteins, etc.), transcription and translation factors, tumor growth inhibitory proteins (e.g., proteins that inhibit angiogenesis), structural proteins (e.g., collagen, fibroin, fibrinogen, elastin, tubulin, actin and myosin), blood proteins (thrombin, VIII, serum albumin, factor VII, insulin, factor IX, factor X, tissue plasminogen activator, protein C, von Wilebrand factor, antithrombin III, glucocerebrosidase, erythropoietin, Granulocyte Colony Stimulating Factor (GCSF) or modified factor VIII, anticoagulants, and the like. In one embodiment, the pharmaceutically active protein according to the invention is a cytokine involved in the regulation of lymphatic homeostasis (lymphoid homeostasis), preferably a cytokine involved in and preferably inducing or enhancing the development, priming, expansion, differentiation and/or survival of T-cells. In one embodiment, the cytokine is an interleukin, such as IL-2, IL-7, IL-12, IL-15, or IL-21.
Another suitable protein of interest encoded by an open reading frame is an inhibitor of Interferon (IFN) signaling. Although it has been reported that the viability of cells into which RNA has been introduced for expression can be reduced, particularly if the cells are transfected multiple times with RNA, IFN inhibitors have been found to enhance the viability of cells in which RNA is to be expressed (WO 2014/071963 a 1). Preferably, the inhibitor is an inhibitor of IFN type I signaling. Preventing IFN receptor engagement by extracellular IFN and inhibiting intracellular IFN signaling allows for stable expression of RNA in cells. Alternatively or additionally, preventing IFN receptor engagement by extracellular IFN and inhibiting intracellular IFN signaling enhances survival of the cells, particularly if the cells are repeatedly transfected with RNA. Without wishing to be bound by theory, it is contemplated that intracellular IFN signaling may lead to inhibition of translation and/or RNA degradation. This can be addressed by inhibiting one or more IFN-inducible antiviral activity effector proteins. The IFN-inducible antiviral activity effector protein may be selected from the group consisting of RNA-dependent Protein Kinase (PKR), 2 ', 5' -oligoadenylate synthase (OAS) and RNaseL. Inhibiting intracellular IFN signaling may comprise inhibiting a PKR-dependent pathway and/or an OAS-dependent pathway. Suitable proteins of interest are proteins capable of inhibiting the PKR-dependent pathway and/or the OAS-dependent pathway. Inhibiting the PKR-dependent pathway can include inhibiting elF2- α phosphorylation. Inhibiting PKR can comprise treating the cell with at least one PKR inhibitor. The PKR inhibitor may be a viral inhibitor of PKR. A preferred viral inhibitor of PKR is vaccinia virus (vaccinia virus) E3. Intracellular expression of a peptide or protein (e.g., E3, K3) is preferred if the peptide or protein is to inhibit intracellular IFN signaling. Vaccinia virus E3 is a 25kDa dsRNA-binding protein (encoded by gene E3L) that binds and sequesters dsRNA to prevent activation of PKR and OAS. E3 can bind directly to PKR and inhibit its activity, resulting in reduced phosphorylation of eIF2- α. Other suitable inhibitors of IFN signaling are herpes simplex virus ICP34.5, toscarna virus (Toscana virus) NS, Bombyx mori nuclear polyhedrosis virus PK2 and HCV NS 34A.
In one embodiment, the inhibitor of intracellular or extracellular IFN signaling is encoded by a replicon. Replicons contain nucleic acid sequence elements that allow replication by alphavirus replicase, typically
Another suitable protein of interest encoded by an open reading frame is a functional alphavirus nonstructural protein. The term "alphavirus nonstructural proteins" includes various and every co-or post-translational modified form of alphavirus nonstructural proteins, including carbohydrate modified (e.g., glycosylated) and lipid modified forms.
In some embodiments, the term "alphavirus non-structural protein" refers to any one or more individual non-structural proteins of alphavirus origin (nsP1, nsP2, nsP3, nsP4), or to a polyprotein comprising polypeptide sequences of more than one non-structural protein of alphavirus origin. In some embodiments, "alphavirus non-structural protein" refers to nsP123 and/or
In some embodiments, the alphavirus non-structural protein is capable of forming a complex or associate, for example, in a host cell. In some embodiments, an "alphavirus non-structural protein" refers to a complex or association of nsP123 (synonymous with P123) and
The term "complex" or "association" refers to two or more identical or different protein molecules in spatial proximity. The proteins of the complex are preferably in direct or indirect physical or physicochemical contact with each other. The complex or association may be composed of a plurality of different proteins (heteromultimers) and/or a plurality of copies of a particular protein (homomultimers). In the case of alphavirus nonstructural proteins, the term "complex or associate" describes a large number of at least two protein molecules, at least one of which is an alphavirus nonstructural protein. The complex or association may be composed of multiple copies of a particular protein (homomultimer) and/or multiple different proteins (heteromultimer). In the case of multimers, "multiple" means more than one, e.g., two, three, four, five, six, seven, eight, nine, ten, or more than ten.
The term "functional alphavirus nonstructural protein" includes alphavirus nonstructural proteins having replicase function. Thus, a "functional alphavirus nonstructural protein" includes an alphavirus replicase. "replicase function" includes the function of an RNA-dependent RNA polymerase (RdRP), i.e., an enzyme capable of catalyzing the synthesis of negative-strand RNA based on a (+) strand RNA template, and/or an enzyme capable of catalyzing the synthesis of (+) strand RNA based on a negative-strand RNA template. Thus, the term "functional alphavirus non-structural protein" may refer to a protein or complex that synthesizes a negative-strand RNA using a (+) strand (e.g., genomic) RNA as a template, a protein or complex that synthesizes a new (+) strand RNA using a negative-strand of genomic RNA as a template, and/or a protein or complex that synthesizes a subgenomic transcript using a fragment of the negative-strand of genomic RNA as a template. Functional alphavirus nonstructural proteins may additionally have one or more additional functions, for example, proteases (for self-cleavage), helicases, terminal adenyltransferases (for poly (a) tail addition), methyltransferases and guanylyl transferases (for providing nucleic acids with a 5' cap), nuclear localization sites, triphosphatases (Gould et al, 2010, Antiviral res., vol 87, p 111 to 124; Rupp et al, 2015, j.gen.virol., vol 96, p 2483 to 500).
According to the present invention, the term "alphavirus replicase" refers to an alphavirus RNA-dependent RNA polymerase, which includes RNA-dependent RNA polymerases from naturally occurring alphaviruses (alphaviruses found in nature) and RNA-dependent RNA polymerases from variants or derivatives of alphaviruses (e.g. from attenuated alphaviruses). In the context of the present invention, the terms "replicase" and "alphavirus replicase" are used interchangeably unless the context dictates that any particular replicase is not an alphavirus replicase.
The term "replicase" includes all variants of alphavirus replicase (in particular, post-translationally modified variants, conformations, isoforms and homologues) which are expressed by cells infected with alphavirus or which are expressed by cells which have been transfected with nucleic acid encoding an alphavirus replicase. Furthermore, the term "replicase" includes all forms of replicase that have been produced, or that can be produced by recombinant means. For example, replicase may be produced by recombinant means comprising a tag (e.g., a myc tag, an HA tag, or an oligohistidine tag (His tag)) that facilitates detection and/or purification of the replicase in the laboratory.
Optionally, the alphavirus replicase is additionally functionally defined by the ability to bind to any one or more of: an alphavirus conserved sequence element 1(CSE 1) or its complement, a conserved sequence element 2(CSE2) or its complement, a conserved sequence element 3(CSE 3) or its complement, and a conserved sequence element 4(CSE 4) or its complement. Preferably, the replicase is capable of binding to CSE 2[ i.e.to (+) strand ] and/or to CSE 4[ i.e.to (+) strand ], or to the complement of CSE 1 [ i.e.to (-) strand ] and/or to the complement of CSE3 [ i.e.to (-) strand ].
The source of replicase is not limited to any particular alphavirus. In a preferred embodiment, the alphavirus replicase comprises non-structural proteins from semliki forest viruses including naturally occurring semliki forest viruses and variants or derivatives of semliki forest viruses, such as attenuated semliki forest viruses. In an alternative preferred embodiment, the alphavirus replicase comprises non-structural proteins from sindbis virus, including naturally occurring sindbis virus and variants or derivatives of sindbis virus, such as attenuated sindbis virus. In an alternative preferred embodiment, the alphavirus replicase comprises non-structural proteins from Venezuelan Equine Encephalitis Virus (VEEV) including naturally occurring VEEV and variants or derivatives of VEEV, such as attenuated VEEV. In an alternative preferred embodiment, the alphavirus replicase comprises non-structural proteins from chikungunya virus (CHIKV), including naturally occurring CHIKV and variants or derivatives of CHIKV, such as attenuated CHIKV.
The replicase may also comprise non-structural proteins from more than one alphavirus. Thus, heterologous complexes or associates comprising non-structural proteins of the alphavirus and having replicase function are also encompassed by the present invention. For illustrative purposes only, the replicase may comprise one or more non-structural proteins from a first alphavirus (e.g., nsP1, nsP2) and one or more non-structural proteins from a second alphavirus (nsP3, nsP 4). Nonstructural proteins from more than one different alphavirus may be encoded by separate open reading frames, or may be encoded as a polyprotein from a single open reading frame, such as nsP 1234.
In some embodiments, the functional alphavirus non-structural protein is capable of forming a membrane replication complex and/or vacuole (vacuole) in a cell expressing the functional alphavirus non-structural protein.
If a functional alphavirus nonstructural protein (i.e.an alphavirus nonstructural protein with replicase function) is encoded by a nucleic acid molecule according to the invention, it is preferred that the subgenomic promoter of the replicon, if present, is compatible with said replicase. In this case, compatible means that the alphavirus replicase is able to recognize the subgenomic promoter (if present). In one embodiment, this is achieved when the subgenomic promoter is native to the alphavirus from which the replicase is derived (i.e., the natural source of these sequences is the same alphavirus). In an alternative embodiment, the subgenomic promoter is not native to the alphavirus from which the alphavirus replicase is derived, provided that the alphavirus replicase is capable of recognizing the subgenomic promoter. In other words, the replicase is compatible with subgenomic promoters (cross-viral compatibility). Examples of cross-viral compatibility with subgenomic promoters and replicases derived from different alphaviruses are known in the art. Any combination of subgenomic promoters and replicase is possible, as long as cross-viral compatibility exists. One skilled in the art can readily test for cross-viral compatibility by incubating the replicase to be tested with RNA having the subgenomic promoter to be tested under conditions suitable for synthesis of RNA from the subgenomic promoter. If a subgenomic transcript is prepared, the subgenomic promoter is determined to be compatible with the replicase. Various examples of cross-viral compatibility are known (reviewed by Strauss & Strauss, microbiol. rev., 1994, volume 58, pages 491 to 562).
In the present invention, the open reading frame encoding a functional alphavirus nonstructural protein may be provided on an RNA replicon, or alternatively, may be provided as a separate nucleic acid molecule (e.g., an mRNA molecule). The individual mRNA molecules may optionally comprise, for example, a cap, a5 '-UTR, a 3' -UTR, a poly (a) sequence, and/or adaptations of codon usage. As described herein, individual mRNA molecules can be provided in trans.
When an open reading frame encoding a functional alphavirus nonstructural protein is provided on an RNA replicon, the replicon may preferably be replicated by the functional alphavirus nonstructural protein. In particular, an RNA replicon encoding a functional alphavirus nonstructural protein may be replicated by a functional alphavirus nonstructural protein encoded by the replicon. This embodiment is highly preferred when the nucleic acid molecule encoding a functional alphavirus nonstructural protein is not provided in trans. In this embodiment, cis replication of the replicon is intended to be achieved. In a preferred embodiment, the RNA replicon comprises an open reading frame encoding a functional alphavirus nonstructural protein and at least one further open reading frame encoding a protein of interest and is replicable by the functional alphavirus nonstructural protein.
If multiple open reading frames are present on the replicon, functional alphavirus non-structural proteins may be encoded by either of them, optionally under or without control of a subgenomic promoter, preferably without control of a subgenomic promoter. In a preferred embodiment, the functional alphavirus non-structural proteins are encoded by the most upstream open reading frame of the RNA replicon. When the functional alphavirus nonstructural proteins are encoded by the most upstream open reading frame of an RNA replicon, the genetic information encoding the functional alphavirus nonstructural proteins will be translated early after introduction of the RNA replicon into the host cell, and the resulting proteins can then drive replication and optionally produce subgenomic transcripts in the host cell.
The presence of an open reading frame encoding a functional alphavirus nonstructural protein comprised by the replicon or comprised by a separate nucleic acid molecule provided in trans allows the replicon to replicate and, thus, express the gene of interest encoded by the replicon at high levels, optionally under the control of a subgenomic promoter.
The RNA replicon is suitable for expressing one or more genes encoding a peptide or protein of interest, optionally under the control of a subgenomic promoter. Various embodiments are possible. One or more open reading frames may be present on the RNA replicon, each encoding a peptide of interest or a protein of interest. The most upstream open reading frame of the RNA replicon is referred to as the "first open reading frame". In some embodiments, the "first open reading frame" is the only open reading frame of an RNA replicon. Optionally, one or more additional open reading frames may be present downstream of the first open reading frame. One or more additional open reading frames downstream of the first open reading frame may be referred to as "second open reading frame", "third open reading frame", etc., in the order in which they are present downstream of the first open reading frame (5 'to 3'). Preferably, each open reading frame comprises an initiation codon (base triplet), typically AUG (in RNA molecules), which corresponds to ATG (in corresponding DNA molecules).
If the replicon comprises a3 'replication recognition sequence, it is preferred that all open reading frames are located upstream of the 3' replication recognition sequence.
When an RNA replicon comprising one or more open reading frames is introduced into a host cell, the replicon can be used directly as a template for translation of the first open reading frame. Preferably, the replicon comprises a 5' -cap. This facilitates the expression of the gene encoded by the first open reading frame directly from the replicon.
In some embodiments, at least one open reading frame of the replicon is under the control of a subgenomic promoter (preferably an alphavirus subgenomic promoter). Alphavirus subgenomic promoters are very efficient and are therefore suitable for high levels of heterologous gene expression. Preferably, the subgenomic promoter is a promoter of a subgenomic transcript in an alphavirus. This means that the subgenomic promoter is a promoter that is native to the alphavirus and preferably controls the transcription of the open reading frame encoding one or more structural proteins in the alphavirus. Alternatively, the subgenomic promoter is a variant of a subgenomic promoter of an alphavirus; any variant that functions as a promoter for the transcription of subgenomic RNA in the host cell is suitable. If the replicon comprises a subgenomic promoter, it is preferred that the replicon comprises conserved sequence element 3(CSE 3) or a variant thereof.
Preferably, the at least one open reading frame under the control of the subgenomic promoter is located downstream of the subgenomic promoter. Preferably, the subgenomic promoter controls the production of subgenomic RNA comprising transcripts of the open reading frame.
In some embodiments, the first open reading frame is under the control of a subgenomic promoter. When the first open reading frame is under the control of a subgenomic promoter, its positioning is similar to that of the open reading frame encoding the structural proteins in the alphavirus genome. When the first open reading frame is under the control of a subgenomic promoter, it is preferred that the genes encoded by the first open reading frame are expressible from both the replicon as well as its subgenomic transcript (the latter in the presence of functional alphavirus nonstructural proteins). One or more additional open reading frames, each under the control of a subgenomic promoter, may be present downstream of the first open reading frame under the control of a subgenomic promoter. Genes encoded by one or more additional open reading frames (e.g., by a second open reading frame) can be translated from one or more subgenomic transcripts, each under the control of a subgenomic promoter. For example, an RNA replicon can comprise a subgenomic promoter that controls the production of a transcript encoding a second protein of interest.
In other embodiments, the first open reading frame is not under the control of a subgenomic promoter. When the first open reading frame is not under the control of the subgenomic promoter, the gene encoded by the first open reading frame can be expressed from the replicon. One or more additional open reading frames, each under the control of a subgenomic promoter, may be present downstream of the first open reading frame. The genes encoded by the one or more additional open reading frames can be expressed from the subgenomic transcript.
In cells comprising a replicon according to the invention, the replicon may be amplified by functional alphavirus nonstructural proteins. In addition, if the replicon comprises one or more open reading frames under the control of a subgenomic promoter, it is contemplated that one or more subgenomic transcripts will be made from functional alphavirus nonstructural proteins. Functional alphavirus nonstructural proteins may be provided in trans, or may be encoded by the open reading frame of the replicon.
If the replicon comprises more than one open reading frame encoding a protein of interest, it is preferred that each open reading frame encodes a different protein, e.g., a different pharmaceutically active peptide or protein. For example, the protein encoded by the second open reading frame is different from the protein encoded by the first open reading frame.
In some embodiments, the protein of interest encoded by the first and/or the further open reading frame(s) (preferably by the first open reading frame) is a functional alphavirus nonstructural protein or an inhibitor of IFN signaling, e.g. E3. In some embodiments, the protein of interest encoded by the first and/or additional open reading frames (e.g., by the second open reading frame) is a pharmaceutically active peptide or protein, or a reporter protein.
In one embodiment, the protein of interest encoded by the first open reading frame is a functional alphavirus nonstructural protein. In this embodiment, the replicon preferably comprises a 5' -cap. Particularly when the protein of interest encoded by the first open reading frame is a functional alphavirus nonstructural protein, and preferably when the replicon comprises a 5' -cap, the nucleic acid sequence encoding the functional alphavirus nonstructural protein can be efficiently translated from the replicon, and the resulting protein can then drive replication of the replicon and drive synthesis of the subgenomic transcript. This embodiment may be preferred when additional nucleic acid molecules encoding functional alphavirus nonstructural proteins are not used or are not present with the replicon. In this embodiment, cis replication of the replicon is intended to be achieved.
The compositions described herein can be administered for the treatment of diseases such as those described herein, e.g., diseases associated with an antigen encoded by the administered RNA.
The term "disease" refers to an abnormal condition affecting the body of an individual. A disease is generally interpreted as a medical condition associated with specific symptoms and signs. The disease may be caused by factors originating from an external source, such as an infectious disease, or may be caused by internal dysfunction (e.g., autoimmune disease). In humans, "disease" is generally used more broadly to refer to any condition that causes pain, dysfunction, distress, social problems or death in the affected individual, or that causes similar problems to those persons with whom the individual comes into contact. In this broader sense, it sometimes includes injuries, disabilities, disorders, syndromes, infections, isolated symptoms (isolatedsymptom), abnormal behavior, and atypical changes in structure and function, while in other cases and for other purposes, these may be considered distinguishable categories. Diseases often affect not only an individual's body, but also his mood, as having and accompanying many diseases life can change a person's view of life and the person's personality.
The term "antigen-associated disease" or "disease involving an antigen" refers to any disease in which an antigen is involved, for example a disease characterized by the presence of an antigen. The disease in which the antigen is involved may be an infectious disease, an autoimmune disease or a cancer disease or simply a cancer. As described above, the antigen may be a disease-associated antigen, such as a tumor-associated antigen, a viral antigen, or a bacterial antigen.
The term "infectious disease" refers to any disease that can be transmitted from individual to individual or from organism to organism and is caused by a microbial agent (e.g., the common cold). Infectious diseases are known in the art and include, for example, viral diseases, bacterial diseases or parasitic diseases, which are caused by viruses, bacteria and parasites, respectively. In this regard, the infectious disease may be, for example, hepatitis, sexually transmitted diseases (e.g., chlamydia or gonorrhea), tuberculosis, HIV/acquired immunodeficiency syndrome (AIDS), diphtheria, hepatitis b, hepatitis c, cholera, Severe Acute Respiratory Syndrome (SARS), avian influenza, animal diseases (e.g., foot and mouth disease, small ruminant plague (pest viruses), Porcine reproductive and respiratory syndrome virus), or parasitic diseases (e.g., trypanosomiasis (Chagas), malaria, and the like).
The term "autoimmune disease" refers to any disease in which the body produces an immunogenic (i.e., immune system) response to a component of its own tissue. In other words, the immune system loses its ability to recognize certain tissues or systems in the body as self and targets it and attacks it as if it were foreign. Autoimmune diseases can be divided into those that affect primarily one organ (e.g., hemolytic anemia and anti-immune thyroiditis), and those in which the autoimmune disease process spreads through many tissues (e.g., systemic lupus erythematosus). For example, multiple sclerosis is thought to be caused by T cell attack on the sheaths surrounding brain and spinal nerve fibers. This results in loss of coordination, weakness and blurred vision. Autoimmune diseases are known in the art and include, for example, Hashimoto's thyroiditis, Grave's disease, lupus, multiple sclerosis, rheumatoid arthritis, hemolytic anemia, anti-immune thyroiditis, systemic lupus erythematosus, celiac disease, Crohn's disease, colitis, diabetes, scleroderma, psoriasis, and the like.
The term "cancer disease" or "cancer" refers to or describes a physiological condition in an individual that is typically characterized by unregulated cell growth. Examples of cancer include, but are not limited to, epithelial carcinoma (carcinoma), lymphoma, blastoma, sarcoma, and leukemia. More specifically, examples of such cancers include bone cancer, blood cancer, lung cancer, liver cancer, pancreatic cancer, skin cancer, head and neck cancer, cutaneous or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, cancer of the anal region, stomach cancer, colon cancer, breast cancer, prostate cancer, uterine cancer, cancers of the sexual and reproductive organs, Hodgkin's Disease, esophageal cancer, cancer of the small intestine, cancer of the endocrine system, cancer of the thyroid gland, cancer of the parathyroid gland, cancer of the adrenal gland, sarcoma of soft tissue, cancer of the bladder, cancer of the kidney, cancer of the renal cell, cancer of the renal pelvis, tumors of the Central Nervous System (CNS), cancer of the neuroectodermal layer, tumor of the spinal axis, glioma, meningioma and pituitary adenoma. The term "cancer" according to the present invention also encompasses cancer metastasis.
The term "immune response" relates to the reaction of the immune system, for example, to an immunogenic organism (e.g., a bacterium or virus), cell, or substance. The term "immune response" includes both innate and adaptive immune responses. Preferably, the immune response is associated with activation of immune cells, induction of cytokine biosynthesis and/or antibody production.
Preferably, the immune response induced by the composition of the invention comprises the following steps: activating antigen-presenting cells (e.g., dendritic cells and/or macrophages), presenting antigen or a fragment thereof by said antigen-presenting cells, and activating cytotoxic T cells as a result of such presentation.
The term "immune cell" refers to a cell that is involved in defending the immune system of an individual's body. The term "immune cell" encompasses a specific type of immune cell and its precursors, including leukocytes, including macrophages, monocytes (precursors of macrophages), granulocytes (e.g., neutrophils, eosinophils, and basophils), dendritic cells, mast cells, and lymphocytes (e.g., B cells, T cells, and Natural Killer (NK) cells). Macrophages, monocytes (precursors of macrophages), neutrophils, dendritic cells and mast cells are phagocytic cells.
The term "immunotherapy" relates to the treatment of a disease or disorder by inducing, enhancing or suppressing an immune response. Immunotherapy designed to elicit or enhance an immune response is classified as activating immunotherapy, while immunotherapy that reduces or suppresses an immune response is classified as suppressing immunotherapy. The term "immunotherapy" includes antigen immunization or antigen vaccination, or tumor immunization or tumor vaccination. The term "immunotherapy" also relates to the manipulation of an immune response such that in the case of autoimmune diseases (e.g. rheumatoid arthritis), allergy, diabetes or multiple sclerosis, an inappropriate immune response is modulated to a more appropriate immune response.
The term "immunization" or "vaccination" describes the process of administering an antigen to an individual in order to induce an immune response, e.g. for therapeutic or prophylactic reasons.
The term "therapeutic treatment" or simply "treatment" relates to any treatment that improves the health status and/or prolongs (increases) the lifespan of an individual. The treatment can eliminate the disease in the subject, prevent or slow the onset of the disease in the subject, inhibit or slow the onset of the disease in the subject, reduce the frequency or severity of symptoms in the subject, and/or reduce relapse in a subject currently suffering from or previously suffering from the disease.
The term "prophylactic treatment" or "prophylactic treatment" relates to any treatment intended to prevent the occurrence of a disease in an individual. The terms "prophylactic treatment" or "prophylactic treatment" are used interchangeably herein.
The terms "protect", "prevent", "prophylactic", "preventive" or "protective" relate to the prevention and/or treatment of the occurrence and/or spread of a disease (e.g. a tumor) in an individual. For example, prophylactic administration of immunotherapy, e.g., by administration of a composition of the invention, can protect a recipient individual from the development of tumors. For example, therapeutic administration of immunotherapy (e.g., by administration of a composition of the invention) may prevent the onset of disease, e.g., resulting in inhibition of tumor progression/growth. This includes a slowing down of tumor progression/growth, in particular a destruction of tumor progression, which preferably leads to elimination of the tumor. Therapeutic administration of immunotherapy can protect an individual, for example, from dissemination or metastasis of existing tumors.
The term "individual" or "subject" relates to a vertebrate, in particular a mammal. For example, mammals in the context of the present invention are humans, non-human primates, domesticated mammals (e.g., dogs, cats, sheep, cows, goats, pigs, horses, etc.), laboratory animals (e.g., mice, rats, rabbits, guinea pigs, etc.), and captive animals (e.g., zoo animals). The term "subject" also relates to non-mammalian vertebrates, such as birds (particularly domesticated birds, such as chickens, ducks, geese, turkeys), and to fish (particularly farmed fish, such as salmon or catfish). The term "animal" as used herein also includes humans.
The pharmaceutical agent, e.g., the polymeric complex particles described herein, may be administered in the form of any suitable pharmaceutical composition. The term "pharmaceutical composition" relates to a formulation comprising a therapeutically effective agent or a salt thereof, preferably together with pharmaceutical excipients such as buffers, preservatives and tonicity adjusting agents. The pharmaceutical composition is for treating, preventing, or reducing the severity of a disease or disorder by administering the pharmaceutical composition to an individual. Pharmaceutical compositions are also known in the art as pharmaceutical formulations. The pharmaceutical composition may be administered locally or systemically. In the context of the present invention, the pharmaceutical composition comprises the particles described herein.
The term "systemic administration" refers to the administration of a therapeutically effective agent such that the agent becomes widely distributed in a significant amount throughout the body of an individual and exerts a biological effect. According to the invention, administration is preferably carried out by parenteral administration.
The term "parenteral administration" refers to the administration of a therapeutically effective agent such that the agent does not pass through the intestine. The term "parenteral administration" includes intravenous administration, subcutaneous administration, intradermal administration, or intraarterial administration, but is not limited thereto.
In a particularly preferred embodiment, the composition according to the invention is administered to muscle tissue, such as skeletal muscle. Thus, intramuscular administration (e.g., by intramuscular injection) is the preferred route of administration.
Administration can be accomplished in a variety of ways. In one embodiment, the composition according to the invention is administered by injection. In a preferred embodiment, the injection is performed through a needle. Needleless injection may be used as an alternative.
The pharmaceutical compositions of the present invention may comprise at least one adjuvant. The term "adjuvant" relates to compounds that prolong or enhance or accelerate the immune response when administered to an individual in combination with an antigen or antigenic peptide. It is theorized that adjuvants exert their biological activity by one or more mechanisms including increasing the surface of the antigen, prolonging retention of the antigen in vivo, delaying antigen release, targeting the antigen to macrophages, increasing antigen uptake, enhancing antigen processing, stimulating cytokine release, stimulating and activating immune cells (e.g., B cells, macrophages, dendritic cells, T cells), and non-specifically activating immune cells. Adjuvants comprise heterogeneous groups of compounds, such as oil emulsions (e.g. Freund's adjuvant), mineral compounds (e.g. alum), bacterial products (e.g. Bordetella pertussis toxin) or immunostimulatory complexes. Examples of adjuvants include, but are not limited to, saponin, incomplete freund's adjuvant, complete freund's adjuvant, tocopherol, or alum.
The pharmaceutical compositions according to the invention are generally administered in a "pharmaceutically effective amount" and in a "pharmaceutically acceptable formulation".
The term "pharmaceutically effective amount" refers to an amount that alone or in combination with another dose achieves a desired response or desired effect. In the case of treating a particular disease, the desired response preferably involves inhibiting the progression of the disease. This includes slowing the progression of the disease and in particular interrupting or reversing the progression of the disease. The desired response in the treatment of a disease may also be to delay the onset of the disease or the condition or to prevent its onset. An effective amount of a composition described herein will depend on the condition to be treated, the severity of the disease, the individual parameters of the patient (including age, physiological condition, size and weight), the duration of the treatment, the type of concomitant therapy (if any), the particular route of administration, and the like. Thus, the dosage of administration of the compositions described herein may depend on a variety of such parameters. In the event that the response in the patient is inadequate at the initial dose, higher doses may be used (or an effectively higher dose achieved by a different, more topical route of administration).
The term "pharmaceutically acceptable" refers to the non-toxicity of a substance that does not interact with the active ingredients of a pharmaceutical composition.
The pharmaceutical compositions of the present invention may comprise salts, buffers, preservatives, carriers, and optionally other therapeutic agents. Preferably, the pharmaceutical composition of the present invention comprises one or more pharmaceutically acceptable carriers, diluents and/or excipients.
The term "excipient" is intended to mean all substances in a pharmaceutical composition that are not active ingredients, such as binders, lubricants, thickeners, surfactants, preservatives, emulsifiers, buffers, flavoring agents or coloring agents.
The term "diluent" relates to a diluent (diluting agent) and/or a diluent (diluting agent). Further, the term "diluent" includes any one or more of a fluid, liquid or solid suspension, and/or a mixing medium.
The term "carrier" relates to one or more compatible solid or liquid fillers or diluents suitable for administration to a human. The term "carrier" relates to a natural or synthetic organic or inorganic component which is combined with an active ingredient to facilitate its use. Preferably, the carrier component is a sterile liquid (e.g., water or oil), which includes those sterile liquids derived from mineral oil, animal or vegetable sources (e.g., peanut oil, soybean oil, sesame oil, sunflower oil, and the like). Saline solutions and aqueous dextrose and glycerol solutions can also be used as aqueous carrier compounds.
Pharmaceutically acceptable carriers or diluents for therapeutic use are well known in the Pharmaceutical art and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing co. (A.R Gennaro editor 1985). Examples of suitable carriers include, for example, magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting wax, cocoa butter, and the like. Examples of suitable diluents include ethanol, glycerol and water.
The pharmaceutical carrier, excipient or diluent may be selected with respect to the intended route of administration and standard pharmaceutical practice. The pharmaceutical composition of the present invention may comprise as or in addition to a carrier, excipient or diluent: any suitable binder, lubricant, suspending agent, coating agent and/or solubilizer. Examples of suitable binders include starch, gelatin, natural sugars (e.g., glucose, anhydrous lactose, free-flowing lactose, beta-lactose), corn sweeteners, natural and synthetic gums (e.g., gum arabic, tragacanth) or sodium alginate, carboxymethylcellulose, and polyethylene glycol. Examples of suitable lubricants include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Preservatives, stabilizers, dyes and even flavoring agents may be provided in the pharmaceutical compositions. Examples of preservatives include sodium benzoate, sorbic acid and esters of p-hydroxybenzoic acid. Antioxidants and suspending agents may also be used.
In one embodiment, the composition is an aqueous composition. The aqueous composition may optionally comprise a solute, such as a salt. In one embodiment, the composition is in the form of a lyophilized composition. Lyophilized compositions can be obtained by freeze-drying the corresponding aqueous composition.
The agents and compositions provided herein can be used alone or in combination with other treatment regimens, such as surgery, irradiation, chemotherapy, and/or bone marrow transplantation (autologous, syngeneic, allogeneic, or unrelated).
The invention is described and illustrated in detail by the accompanying drawings and examples, which are for illustrative purposes only and are not intended to be limiting. Other embodiments also encompassed by the present invention will become apparent to the skilled artisan in view of the description and examples herein.