阅读说明：本技术 乳腺癌分子分型及远处转移风险基因群及诊断产品和应用 (Breast cancer molecular typing and distant metastasis risk gene group, diagnosis product and application ) 是由 周彤 胡志元 周伟庆 于 2019-09-30 设计创作，主要内容包括：本发明公开了乳腺癌亚型分型及评估远处转移风险基因群及其体外诊断产品和应用。(The invention discloses a breast cancer subtype typing and remote metastasis risk assessment gene group, and an in-vitro diagnosis product and application thereof.)

A gene cluster for molecular typing of breast cancer and/or assessing the risk of distant metastasis thereof,

the gene group comprises 66 molecular typing and distant metastasis risk assessment related genes, wherein,

the 66 molecular typing and distant metastasis risk assessment related genes comprise:

(1) proliferation-related genes ASPM, AURKA, BIRC5, CCNB1, CDC20, CDK1, CENPU, CEP55, MELK, MKI67, NEK2, PRC1, PTTG1, RRM2, TOP2A, TPX2, TYMS, UBE2C and ZWINT,

(2) immune-related genes APOBEC3G, CCL5, CCR2, CD2, CD3D, CD52, CD53, CORO1A, CXCL9, GZMA, GZMK, HLA-DMA, HLA-DQA1, IL2RG, LCK, LYZ and PTPRC,

(3) basal cell-associated genes ACTR3B, CDH3, EGFR, FOXC1, KRT14, KRT17, KRT5, MIA, MYC, PHGDH, and SFRP1,

(4) estrogen receptor related genes BAG1, BCL2, BLVRA, CD68, ESR1, FOXA1, GSTM1, MAPT, MDM2, MLPH, NAT1, PGR, SCUBE2 and SLC39A6,

(5) HER2 related genes ERBB2, FGFR4 and GRB7,

(6) invasion-associated genes CTSL2 and MMP 11.

The gene cluster according to claim 1,

the gene group further comprises a housekeeping gene;

preferably, the housekeeping genes comprise at least one, preferably at least 3, most preferably 6 of: GAPDH, GUSB, MRPL19, PSMC4, SF3a1, and TFRC.

Use of a population according to claim 1 or 2 for molecular typing of breast cancer and/or assessing the risk of distant metastasis thereof.

Use of a gene population according to claim 1 or 2 for the preparation of a diagnostic product for molecular typing and/or assessing the risk of distant metastasis of breast cancer.

Use of an agent for detecting the expression level of a gene in the gene population according to claim 1 or 2 for the preparation of a diagnostic product for molecular typing of breast cancer and/or for assessing the risk of distant metastasis thereof.

A diagnostic product for molecular typing of breast cancer and/or assessing the risk of distant metastasis thereof, comprising a reagent related to the detection of the expression level of a gene in the gene population of claim 1 or 2.

The use or diagnostic product according to claim 5 or 6, in the form of an in vitro diagnostic product, preferably a diagnostic kit.

Use or diagnostic product according to any of claims 5 to 7, wherein said agent is an agent for measuring the amount of RNA, in particular mRNA, transcribed from said gene.

The use or diagnostic product of any one of claims 5 to 8, wherein said reagent is a reagent for detecting the amount of cDNA complementary to said mRNA.

The use or diagnostic product according to any one of claims 5 to 9, wherein the diagnostic product further comprises a total RNA extraction reagent, a reverse transcription reagent and/or a secondary sequencing reagent.

Use or diagnostic product according to any of claims 5 to 10, wherein said agent is an agent for detecting the amount of a polypeptide encoded by said gene, preferably wherein said agent is an antibody, an antibody fragment or an affinity protein.

The use or diagnostic product of any one of claims 5 to 11, wherein the agent is a probe or primer, preferably a primer.

The use or diagnostic product of claim 12, wherein the primer has a sequence as set forth in SEQ ID No.1 to SEQ ID No. 144.

A set of primers for molecular typing of breast cancer and/or assessing the risk of distant metastasis thereof, wherein the sequence of the primers is shown in SEQ ID No.1-SEQ ID No. 144.

Use of a primer set according to claim 14 for the preparation of a product for molecular typing and/or assessing the risk of distant metastasis of breast cancer.

The panel of genes, uses, diagnostic products or primer sets of any one of claims 1 to 15, wherein said typing of breast cancer comprises luminal a, luminal B, HER2-enriched, basal cell type and immunopotentiating.