阅读说明：本技术 一种适用于Cas12蛋白的缓冲系统及其应用 (Buffer system suitable for Cas12 protein and application thereof ) 是由 段志强 陈莹 于 2021-07-16 设计创作，主要内容包括：本发明提供了一种适用于Cas12蛋白的缓冲系统,所述缓冲系统包括pH缓冲液；二价阳离子；还原剂和稳定剂。其特征在于,所述pH缓冲液是将pH值维持在7.0-9.0的pH缓冲液；所述二价阳离子为镁离子；所述还原剂选自DTT、β-巯基乙醇和TCEP中的一种或任意几种；所述稳定剂选自BSA、甘油和PEG中的一种或任意几种。(The invention provides a buffer system suitable for a Cas12 protein, the buffer system comprising a pH buffer; a divalent cation; reducing agents and stabilizers. Wherein the pH buffer is a pH buffer that maintains the pH at 7.0 to 9.0; the divalent cations are magnesium ions; the reducing agent is selected from one or any of DTT, beta-mercaptoethanol and TCEP; the stabilizer is selected from one or more of BSA, glycerol and PEG.)