阅读说明：本技术 一个表观遗传操作植物表型可塑性性状的方法 (Method for epigenetic manipulation of plant phenotypic plasticity traits ) 是由 强胜 解洪杰 于 2018-03-07 设计创作，主要内容包括：提供一个表观遗传操作植物表型可塑性性状的方法,通过简并密码子定量替换目的基因的可甲基化位点,通过设计合成基因,进行转基因操作；或利用CRISPR/Cas9技术构建植物表达载体进行原位在体基因简并密码子定量替换可甲基化位点,定量调控外源或原位在体目的基因甲基化程度和基因表达水平,达到定量调控目标植物的可塑性性状。通过定量调节ICE1基因的甲基化程度培育不同抗寒能力植物,为通过表观遗传技术培育抗寒作物提供了新的选择,为降低低温伤害对作物产量和品质等的影响提供了技术方法。(Providing a method for epigenetic manipulation of plant phenotypic plasticity traits, quantitatively replacing methylatable sites of a target gene by degenerate codons, and carrying out transgenic manipulation by designing a synthetic gene; or a plant expression vector is constructed by using the CRISPR/Cas9 technology to carry out in-situ in-vivo gene degenerate codon quantitative replacement on the methylatable site, and the methylation degree and the gene expression level of the exogenous or in-situ in-vivo target gene are quantitatively regulated, so that the quantitative regulation of the plasticity of the target plant is achieved. Plants with different cold resistance abilities are cultivated by quantitatively regulating the methylation degree of the ICE1 gene, a new choice is provided for cultivating cold-resistant crops by an epigenetic technique, and a technical method is provided for reducing the influence of low-temperature injury on the yield, quality and the like of the crops.)