阅读说明：本技术 一种用于清除hpv的复合制剂 (It is a kind of for removing the compound formulation of HPV ) 是由 邓兆群 王京苏 王盛洲 柯金铭 周新路 于 2019-09-20 设计创作，主要内容包括：本发明提供一种清除效率高、转阴率效果好的复合制剂。由以下重量配比组成：乳清蛋白0.1-5g,壳聚糖0.01-4g,葡聚糖0.01-5g,茶多酚0.1-3g,溶菌酶0.1-2g,山梨酸钾0.2-2g,聚六亚甲基胍0.01-0.1g,乳鉄蛋白0.1-5g,去离子水60～120ml；本发明抗HPV作用机制多样,清除效率高、转阴率效果好本发明制备复合制剂通过利用乳清蛋白,壳聚糖,葡聚糖,茶多酚,溶菌酶,山梨酸钾,聚六亚甲基胍,乳鉄蛋白等原料配比而成,抗HPV能力强,功能多样,副作用小。(The present invention provides the compound formulation that a kind of elimination efficiency is high, negative conversion rate effect is good.It is made of following weight proportion: lactalbumin 0.1-5g, chitosan 0.01-4g, glucan 0.01-5g, tea polyphenols 0.1-3g, lysozyme 0.1-2g, potassium sorbate 0.2-2g, polyhexamethylene guanide 0.01-0.1g, newborn iron albumen 0.1-5g, 60~120ml of deionized water；The anti-HPV mechanism of action multiplicity of the present invention, elimination efficiency is high, the good present invention of negative conversion rate effect prepares compound formulation by utilizing lactalbumin, chitosan, glucan, tea polyphenols, lysozyme, potassium sorbate, polyhexamethylene guanide, the raw material proportionings such as newborn iron albumen form, anti- HPV ability is strong, vdiverse in function, Small side effects.)

1. a kind of for removing the compound formulation of HPV, which is characterized in that be made of following weight proportion: lactalbumin 0.1-5g, Chitosan 0.01-4g, glucan 0.01-5g, tea polyphenols 0.1-3g, lysozyme 0.1-2g, potassium sorbate 0.2-2g, poly- six methylene Base guanidine 0.01-0.1g, newborn iron albumen 0.1-5g, 60~120ml of deionized water.

2. a kind of for removing the compound formulation of HPV according to claim 1, which is characterized in that by the original of following parts by weight Material composition: lactalbumin 0.5-4g, chitosan 0.05-3g, glucan 0.5-4g, tea polyphenols 0.5-2g, lysozyme 0.5- 1.5g, potassium sorbate 0.6-1.5g, polyhexamethylene guanide 0.02-0.08g, newborn iron albumen 0.5-4g, deionized water 80~ 110ml。

3. a kind of for removing the compound formulation of HPV according to claim 1, which is characterized in that by the original of following parts by weight Material composition: lactalbumin 3g, chitosan 2g, glucan 3g, tea polyphenols 1g, lysozyme 1g, potassium sorbate 1g, polyhexamethylene Guanidine 0.05g, newborn iron albumen 2g, deionized water 90ml.

4. according to claim 1 a kind of for removing the compound formulation of HPV, which is characterized in that the preparation method comprises the following steps:

(1) raw material sterilizes: 60Coradiation sterilizing, and the dose of radiation of sterilization is 1-10kGy；

(2) prepared by sterile water: tap water high pressure sterilization, and 121 DEG C, the sterilizing of 103.4kPa vapour pressure maintains 30min, spare；

(3) just formulation ingredients mix: autoclaved sterile water is heated to 50 degree, by chitosan stirring and dissolving, is separately added into cream Albumin, glucan, tea polyphenols, lysozyme, potassium sorbate, newborn iron albumen, stirring and dissolving mix；

(4) just preparation filters: will stir and evenly mix mixture and is fixed on tube sheet, is packed into pressure-resistant staving, filter area is in 3- 30m², operating pressure is the PE filter membrane filtering of > 0.1um in 0.2Mpa. filtering accuracy, filters liquid under collection membrane；

(5) product dispenses: filtering liquid is sub-packed in 50-200ml sterile chamber.

5. a kind of for removing the compound formulation of HPV as claimed in claim 4, it is characterised in that: the preparation method of PE filter membrane Are as follows:

(1) it sieves: PE powder is sieved with the sieving machine that aperture is 5-8 microns；

(2) mix: curing agent and surfactant is added in the PE powder after screening, and stirring forms mixed-powder, adds into mold Enter mixed-powder；

(3) it is sintered: the mold for the mixed-powder being equipped in step (2) is kept into 120- under conditions of 80-90 DEG C, 2-4MPa 180min sinter molding is cooled to room temperature and obtains PE skeleton；

(4) it takes step (1) screening to obtain PE powder, 3-7 times of solvent is added and obtains PE liquid；

(5) it is poured: controlling pressure 5-8MPa in the skeleton that PE injection step (3) is obtained, 130-180 DEG C of temperature, be cooled to room Temperature both a kind of PE film.

6. the preparation method of PE film as claimed in claim 5: curing agent in step (2) is m-phenylene diamine (MPD): diamines: two propylene Triamine is mixed by weight 3:5-7:3-5.

7. the preparation method of PE film as claimed in claim 5: surfactant is by lecithin in step (2): stearic acid: poly- sorb Ester is mixed by weight 1:3-6:3-4.

8. the preparation method of PE film as claimed in claim 5: the solvent is polysulfones.

Technical field

The invention belongs to field of pharmaceutical preparations, and in particular to one kind can remove the compound formulation of HPV.

Background technique

Human papilloma virus (Human Papilloma Virus, HPV) is a kind of common skin infection and mucous membrane group The virus knitted, about more than 100 kinds of HPV types.The HPV type of common wart is caused mainly to infect by skin contact, Chang Fasheng In hand, foot position.The HPV of this type can also be infected by sharing towel or other articles with this kind of patient.

Human papilloma virus is a kind of DNA tumour virus, is classified as difference using HPV viruse DNA sequence analysis technology Type, it has now been found that more than 130 kinds of types [1].According to different type HPV and tumorigenic risk height, by it It is divided into high-risk-type and low risk.International Cancer Research Center clearly proposes that high-risk HPV virus infection is the master of uterine neck carcinogenesis Want factor.It is reported that the new hair cases of cervical cancer in annual about 500,000 of the whole world, more than 20 ten thousand people die of cervical carcinoma, seriously endanger The health and lives of women [2].Develop into invasive carcinoma of cervix to the end from initial infection high-risk HPV virus, needs 10 ~15 years.Therefore, actively intervened after discovery cervical infection high-risk HPV virus, disease can be blocked in time Further development, achieve the purpose that prevent uterine neck carcinogenesis.

Cervix cancer is mainly caused by high-risk human mammilla papillomavirus (HPV) persistent infection, in 99.7% cervix cancer High-risk HPV all can be detected.HPV infection has apparent correlation (OR=3.6-254.2), especially HPV16 with cervical carcinoma Type and 18 types.International Agency for Research on Cancer (IARC) announce the results show that the whole world in 2018, which is estimated to be nearly 570,000 women, newly suffers from son Cervical carcinoma, more than 310,000 women die of cervix cancer.New hair cases of cervical cancer number about 110,000 of China in 2018, annual death Nearly 50,000 people.

HPV infection genital tract is a long-term process, can be hidden the several years in the cell, once immunity of organisms drops Low, latent virus can reactivate.HPV infection process is generally divided into latent infection phase, subclinical infection phase, clinical stage Tumor stage relevant with HPV.Cervical carcinoma also has a series of precursor lesion, i.e. epithelium of cervix uteri atypical hyperplasia, is pathologically claiming Cervical intraepitheliaI neoplasia (CIN) is usually divided into three-level according to severity again: slight tumor becomes (CINI), uterine neck in epithelium of cervix uteri Upper intradermal moderate tumor becomes (CINII) and epithelium of cervix uteri inner height tumor becomes (CINIII), these precancerous lesions are possible to development and are Invasive carcinoma of cervix.

Doctor trained in Western medicine to the therapeutic strategy of cervical HPV infection with prevention of diseae, both disease was anti-becomes thinking, cervical HPV it is preventative And therapeutic vaccine is expected to thoroughly eliminate HPV viruse.Temporization can be used in patient for having infected HPV viruse, but Many patients are unwilling passively to wait, and are more willing to take positive remedy measures, doctor trained in Western medicine is to eliminate virus, destroy virus host Based on cell.Chinese medicine has the function of improving immunity of organisms and antiviral, can be reduced the wound of physiotherapy and operation, clinical Upper use Chinese medicine is for oral administration and external treatment of tcm treats this disease, achieves good curative effect, has broad prospects.

The anti-HPV mechanism of action of existing product is single, and anti-HPV effect is limited, is not able to satisfy clinical demand, is badly in need of anti-HPV effect Fruit is good, the new product of Small side effects.Our patented product combines for traditional Chinese and western medicine, and anti-HPV ability is strong, vdiverse in function, mainly By inhibiting the HPV duplication enhancing anti-HPV of four number of mechanisms such as body entirety and local immunity, there is good anti-HPV infection Effect.

[1]de Cremoux P,de la Rochefordière A,Savignoni A,et al.Different outcome of invasive cervical cancer associated with high-risk versus intermediate-risk HPV genotype[J].IntJ Cancer,2009,124(4):778-782.

[2]Syrjanen S,Naud P,Sarian L,et al.Up-regulation of 14-3-3sigma (stratifin)is associated with high-grade CIN and high-risk,human papillomavirus(HPV)at baseline but dose not predict outcomes of HR-HPV infections or incident CIN in the LAMS study[J].Am J Clin Pathol,2010,133(2): 232-240.

Summary of the invention

The problems in technology based on the above background, the present invention is quasi- to develop that a kind of elimination efficiency is high, negative conversion rate effect is good answers Close preparation.

It is a kind of for removing the compound formulation of HPV, be made of following weight proportion: lactalbumin 0.1-5g, chitosan 0.01-4g, glucan 0.01-5g, tea polyphenols 0.1-3g, lysozyme 0.1-2g, potassium sorbate 0.2-2g, polyhexamethylene guanide 0.01-0.1g, newborn iron albumen 0.1-5g, 60~120ml of deionized water.

Preferably, a kind of for removing the compound formulation of HPV, it consists of the following raw materials in parts by weight: lactalbumin 0.5-4g, chitosan 0.05-3g, glucan 0.5-4g, tea polyphenols 0.5-2g, lysozyme 0.5-1.5g, potassium sorbate 0.6- 1.5g, polyhexamethylene guanide

0.02-0.08g, newborn iron albumen 0.5-4g, 80~110ml of deionized water.

Preferably, a kind of for removing the compound formulation of HPV, it consists of the following raw materials in parts by weight: lactalbumin 3g, Chitosan 2g, glucan 3g, tea polyphenols 1g, lysozyme 1g, potassium sorbate 1g, polyhexamethylene guanide 0.05g, newborn iron albumen 2g, Deionized water 90ml.

It is a kind of for removing the preparation method of the compound formulation of HPV, comprising the following steps:

(1) raw material sterilizes: 60Coradiation sterilizing, and the dose of radiation of sterilization is 1-10kGy；

(2) prepared by sterile water: tap water high pressure sterilization, and 121 DEG C, the sterilizing of 103.4kPa vapour pressure maintains 30min, spare；

(3) just formulation ingredients mix: autoclaved sterile water is heated to 50 degree, by chitosan stirring and dissolving, respectively plus Enter lactalbumin, glucan, tea polyphenols, lysozyme, potassium sorbate, newborn iron albumen, stirring and dissolving, mixing；

(4) just preparation filters: will stir and evenly mix mixture and is fixed on tube sheet, is packed into pressure-resistant staving, filter area exists 3-30m², operating pressure is the PE filter membrane filtering of > 0.1um in 0.2Mpa. filtering accuracy, filters liquid under collection membrane；

(5) product dispenses: filtering liquid is sub-packed in 20-200ml sterile chamber.

The PE filter membrane the preparation method comprises the following steps:

(1) it sieves: PE powder is sieved with the sieving machine that aperture is 5-8 microns；

(2) mix: curing agent and surfactant is added in the PE powder after screening, and stirring forms mixed-powder, to mold Middle addition mixed-powder；

(3) it is sintered: the mold for the mixed-powder being equipped in step (2) is kept under conditions of 80-90 DEG C, 2-4MPa 120-180min sinter molding is cooled to room temperature and obtains PE skeleton；

(4) it takes step (1) screening to obtain PE powder, 3-7 times of solvent is added and obtains PE liquid；

(5) it is poured: controlling pressure 5-8MPa in the skeleton that PE injection step (3) is obtained, it is 130-180 DEG C of temperature, cooling To room temperature both a kind of PE film.

As further limiting, the curing agent in step (2) is m-phenylene diamine (MPD): diamines: dipropylenetriamine is by weight 3:5-7:3-5 is mixed.

As further limiting, surfactant is by lecithin in step (2): stearic acid: polysorbate is by weight 1: 3-6:3-4 is mixed.

As further limiting, step (4) solvent is polysulfones.

Each main functional component mechanism of action of the present invention is as follows:

Lactalbumin has antibacterial and antiviral functions^[3].Previously, it had been considered in lactalbumin lactoferrin antibacterial action Mainly remove iron ion (remove enteron aisle in iron ion) required for part pathogenic bacteria grow in vivo, and bovine lactoferrin and Its newborn iron transporter for being digested generation in vivo can directly destroy the okioplast wall of Gram-negative bacteria, lead to cell Integrity violations, it is final dead.The antibacterial action of newborn iron operating albumen is stronger than lactoferrin, it can inhibit a variety of and cause a disease The growth and breeding of bacterium, such as Escherichia coli, Bacterium enteritidis, Friedlander's bacillus and campylobacter jejuni.Lactalbumin Also have and adjusts immune and oxidation resistant effect.Through Cell culture invitro test and animal vivo test discovery, lactalbumin can Body non-specificity and specific immune response are improved, the generation of chemically induced property cancer can be prevented, and can also effectively subtract The pressure that slow body tissue aoxidizes^[4].Lactalbumin has the principle and antioxidant mechanism of facilitation to immune function All related rich in cysteine and glutamic acid with it, both amino acid are all the premises of internal synthesizing glutathion.Work as intake After lactalbumin rich in above two amino acid, the concentration of body tissue glutathion inside can be improved, and glutathione is body " street cleaner " of interior free radical.

Lysozyme, the enzyme with dissolution of bacteria cell wall^[5], it is a kind of alkaline protein, under acidic environment, enzymatic activity is very By force.Lysozyme has the function of antibacterial anti-inflammatory, strengthen immunity.

Chitosan is a kind of derivative of chitin --- deacetylated product dissolves in acidic aqueous solution.In human body It is biodegradable, it has no toxic side effect, not only can be used as pharmaceutical carrier but also can be used as pharmaceutical film and dressing, thus led in medicine Domain is widely used^[6], chitosan have the effects that it is antibacterial, hemostasis, analgesia.

Glucan, refers to the homotype polysaccharide formed using glucose as monosaccharide, with glucosides key connection between glucose unit.It Can activated macrophage, neutrophils etc., therefore containing for leukin, the basic element of cell division and distinct antibodies can be improved Amount stimulates the immune system of body comprehensively.So, body, which just has, is more ready to disease caused by resisting microorganism.β-Portugal The ability that glycan can make the lymphocyte of injured body generate cell factor (IL-1) is restored rapidly normally, effectively adjusts body and exempts from Epidemic disease function.Many experiments show that beta glucan can promote the generation of internal IgM antibody, to improve the immunocompetence of body fluid.It is this The cell of glucan activation can excite the non-specific defense mechanism of host, therefore apply in terms of tumour, infection disease and treatment wound It attracts attention deeply.It is extracted through specific step and has assert it is a kind of safe object in U.S. FDA without endotoxic β -1,3 glucan Matter can be added in normal food, and many reports show that mouse takes orally yeast β -1, and 3 glucans can increase strong peritoneal cell antibacterial Phagocytosis.In addition, glucan, which still has, removes free radical, anti-radiation, dissolving cholesterol, the effect of prevention hyperlipidemia and resistance It is infected caused by filterable virus, fungi, bacterium etc.^[7].Therefore it is widely used in the industries such as medicine, food, cosmetics.

Tea polyphenols have apparent control efficiency to following disease: blood pressure lowering, anticoagulation, reducing blood lipid is fat, prevention and treatment artery is hard Change and the cardiovascular diseases such as thrombosis, hypoglycemic, prevention and treatment diabetes, sterilizing and anti-virus；Prevent and treat gastrointestinal tract, respiratory tract, influenza etc. Disease；Prevent and treat hepatitis, anti-aging and enhancing immunity function；The curative effects such as strong tooth, prevention and treatment periodontitis, anti-caries, smelly eliminating removing toxic substances.Tea is more Phenol can be used as the paramedicines of above-mentioned Disease and the raw material of health medicine^[8].Tea polyphenols are as a kind of wide spectrum, potent, low The antimicrobial of poison is recognized by the scholar of many countries in the world.

Polyhexamethylene guanide --- environment-friendly type macromolecule polymer disinfection sanitizer has broad-spectrum；Effective concentration is low； Work fast；Property is stablized；Excellent performance soluble easily in water；It can use at normal temperature；It is long-term antibacterial, without side-effects；Without corruption Corrosion；It is colourless, odorless；It is nontoxic；It is non-ignitable, not quick-fried, using safe；It is moderate；Convenient transportation, it may be said that be optimal sterilization Agent.In fact, the product is a kind of environment-friendly multipurpose new polymers, have in industrial, agriculture, medical and daily life Extremely extensive purposes.

Beneficial effects of the present invention:

(1) anti-HPV mechanism of action multiplicity, elimination efficiency is high, the good present invention of negative conversion rate effect prepares compound formulation and passes through benefit With lactalbumin, chitosan, glucan, tea polyphenols, lysozyme, potassium sorbate, polyhexamethylene guanide, the raw materials such as newborn iron albumen match Than forming, the anti-HPV ability of the product is strong, vdiverse in function, Small side effects.It is main by inhibiting HPV duplication enhancing body whole and The anti-HPV of four number of mechanisms such as local immunity has the effect of good anti-HPV infection.

(2) Small side effects, raw materials used this product is mostly food and raw material, to human body Small side effects.

Specific embodiment