阅读说明：本技术 一种以蚕蛹为原料制备免消化蚕蛹窝蛋白质的方法 (Method for preparing digestion-free silkworm pupa pit protein by using silkworm pupas as raw materials ) 是由 朱振伟 朱光 于 2021-03-12 设计创作，主要内容包括：本发明公开了一种以蚕蛹为原料制备免消化蚕蛹蛋白质的方法,发明所属生物食品,利用蚕蛹为原料,在体外通过生物酶解技术完成人体内的蛋白质消化,生产出免消化蚕蛹蛋白质产品。免消化蛋白质即指分子量在500Da(道尔顿)小分子的蛋白质,其中平均分子量在120Da的复合氨基酸和分子量在180—500Da的小分子二肽与三肽。海参的蛋白质是比较好的优质蛋白质,但再好的蛋白质都要靠人体消化到能吸收的状态才可以被人体吸收。由于老年人随着年龄的增加,身体各功能都在老化及减退,特别是消化功能,所以蛋白质的消化不足使蛋白质的利用率在降低,损失和浪费了很多蛋白质资源,免消化蛋白质产品的开发对老年人补充蛋白质营养不足有很大意义。(The invention discloses a method for preparing digestion-free silkworm pupa protein by using silkworm pupas as raw materials, which belongs to biological food. Indigestible protein refers to proteins with small molecules of molecular weight of 500Da (Dalton), wherein the average molecular weight is 120Da and the molecular weight is 180-500 Da, such as dipeptide and tripeptide. The protein of the sea cucumber is better high-quality protein, but the better protein can be absorbed by the human body only by being digested to be in an absorbable state by the human body. As the old people age, all the functions of the body are aged and declined, particularly the digestion function, the utilization rate of the protein is reduced due to the insufficient digestion of the protein, a lot of protein resources are lost and wasted, and the development of the digestion-free protein product has great significance for supplementing insufficient protein nutrition for the old people.)

1. A method for preparing digestion-free cubilose protein by taking cubilose as a raw material is characterized by comprising the following steps: the bird's nest is used as a protein raw material, deep enzymolysis is carried out, small molecular proteins with the molecular weight below 500 daltons are obtained, and the small molecular proteins can be directly absorbed by small intestines and enter a body, wherein the small molecular proteins comprise compound amino acids with the average molecular weight of 120 daltons, small molecular dipeptides with the molecular weight of 180-500 daltons and tripeptides;

the preparation process comprises the following steps:

firstly, cleaning silkworm chrysalis raw materials, crushing the silkworm chrysalis raw materials by using water in a ratio of 1 to 8-10 times, and thinning silkworm chrysalis materials;

secondly, sterilizing and modifying the refined silkworm chrysalis feed liquid, and instantaneously treating the silkworm chrysalis feed liquid at 125-130 ℃ for 10-16 seconds;

thirdly, the sterilized silkworm chrysalis feed liquid after the protein denaturation treatment is required to be processed at a temperature of between 54 and 56 ℃;

fourthly, adding 0.3 to 0.5 percent of compound protease into the cooled feed liquid, slowly stirring the mixture, controlling the temperature to be between 54 and 56 ℃, carrying out deep enzymolysis for 36 to 72 hours or more until the mixture reaches below 500 daltons and meets the product standard, and not adjusting the pH value in the whole enzymolysis process;

fifthly, centrifugally separating the feed liquid after the enzymolysis is finished to remove insoluble substances;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to kill protease;

seventhly, concentrating the solid material content of the materials to be more than 40 percent by using a double-effect evaporator respectively, and performing spray drying to obtain a digestion-free silkworm chrysalis protein powder product;

and sixthly, mixing the liquid materials respectively sterilized and inactivated by the step sixthly according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free silkworm chrysalis protein liquid product.

2. The method for preparing digestion-free silkworm pupa protein by using cubilose as the raw material according to claim 1, which is characterized in that a digestion-free silkworm pupa protein product with the molecular weight of below 500 daltons is produced by using silkworm pupa protein as the raw material through complex enzyme digestion.

3. The method for preparing digestion-free silkworm pupa protein from silkworm pupas as claimed in claim 1, wherein the digestion-free silkworm pupa protein comprises the following steps: firstly, cleaning the silkworm chrysalis raw material, crushing the silkworm chrysalis raw material by using water of which the ratio is 1 to 8-10 times, and refining the silkworm chrysalis raw material liquid;

secondly, sterilizing and modifying the refined silkworm chrysalis feed liquid, and instantaneously treating the silkworm chrysalis feed liquid at 125-130 ℃ for 10-16 seconds;

thirdly, the silkworm chrysalis feed liquid after sterilization and protein denaturation is cooled to 54-56 ℃;

fourthly, adding 0.3 to 0.5 percent of compound protease into the cooled feed liquid, slowly stirring the mixture, controlling the temperature to be between 54 and 56 ℃ all the time, and deeply performing enzymolysis for 36 to 72 hours to meet the standard requirement of products below 500 daltons, wherein the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, centrifugally separating the feed liquid after the enzymolysis is finished to remove insoluble substances;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to kill protease;

seventhly, concentrating the solid material content of the material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free silkworm chrysalis protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated liquid material according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free silkworm pupa protein liquid product.

Technical Field

The invention relates to a preparation method of biological food, in particular to a method for preparing digestion-free silkworm pupa protein by using silkworm pupas as raw materials.

Background

According to the latest theories of life and physiology: when the human body is about 25 years old, the growth period is over 30 years old, and the maturity period is over, all the functions of the human body can begin to decline, and the functions decline greatly after 60 years old. The new theory suggests that the main reason of aging of the old people is that endogenous cellular proteins dropped in intestines cannot be completely recycled, and the long-term shortage of body proteins is the main reason of premature aging of the old people. The physiology states that: the life cycle of human intestinal cells is 43 hours at most, the life cycle of cells on the surface of the stomach is three days, old cells are exfoliated as new cells grow out, the cells are digested and utilized by the human body like food, and normal people have 8-inch and two-inch exfoliated cells, namely 248.8 g of intestinal cell proteins are exfoliated every day. Half jin of human body cell protein with the biological value of 100 is the best quality, the falling cell protein of the stomach is not considered, the cell protein of the small intestine is not denatured by gastric acid, the molecule winding is very tight and is not easy to digest, and the normal young people can completely recycle the protein after the long-time digestion at the lower end of the small intestine.

The human protein supply consists of three parts:

the first part supplies: is cell protein dropped from intestinal tract, the dropped cell is digested and utilized like food, adult has at least 8 engbi and 31.1 g per engbi every day, namely 248.8 g of dropped cell protein, the cell protein is human protein, is the best quality protein with 100 biological potency, and the biological potency of any other kind of protein is lower than the best quality protein.

A second part supplying: is dead cells in tissues, about 198-283 g of dead cells are repeatedly used by autophagy of human bodies every day, and autophagy means that the dead cells eat themselves. The existence of enzymes in living human cells that digest proteins, carbohydrates and fats can digest apoptotic cells for reuse by the cells is called autophagy. Looking up "one day hungry secret" autophagy science on the web, doctor university swedish japan scientist in 2016 thus won the nobel prize.

The third part supplies: is protein in food, and is supplemented by 1.0 g to 1.5 g per kilogram of body weight per day. The protein in food is digested in stomach, the protease secreted by human stomach is inactive and activated by gastric acid to digest the protein in food, and the aging of human body is the decrease of secretion in body which directly affects the digestion of protein in body.

The old people can not completely recycle endogenous cell protein due to the aging of each function of the organism, especially the reduction of the secretion amount and the reduction of the activity of digestive enzyme, so that the long-term nitrogen source imbalance of the organism is caused. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle so badly that the body cells use their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the old has no way, the digestive function of the body is reduced, and the protein in the body is not digested, namely the protein is absorbed and utilized. And the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole, and the toxins are absorbed by the large intestine to hurt the body. The digestion-free protein product is the only method for solving the problem of protein deficiency of the old. Protein nutrition plays an important role in the aging process, and nutrient imbalance not only accelerates organ aging, but also causes diseases. Another manifestation of aging in the elderly is: the metabolism is slow, the digestion function is reduced, in particular, various hormones in the body are reduced, the hormones are generated by the human body, the basic substances of the hormones are all proteins, including immune cells, so that protein nutrients are absolutely indispensable and are the most important substances in life.

The indigestible protein is a protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered during the study of genes that the human body has a substance that determines all functional activities of the human body's life, called peptides. He speaks: peptide is a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been undergoing three health revolution since history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, the third was the use of small molecule active peptide to treat all diseases including all major diseases, which was the problem of cell failure. Medical science states that only one disease of humans is cellular failure. Cell failure is due to two causes, 1, the cells are not nourished. (not getting the needed things) 2, cytotoxic overload. (unwanted material is obtained).

The silkworm pupa has a protein content of more than 50 percent, which is far higher than that of common food, and the types of necessary amino acids in the cut protein are complete. The silkworm pupa protein consists of 18 amino acids, wherein the content of 8 amino acids necessary for human bodies is high, is 2 times that of pork, 4 times that of eggs, 10 times that of milk, and 8 amino acids necessary for human bodies are balanced in nutrition and proper in proportion, and meets the requirements of FFFAO/WHO (grain and agriculture organization and world health organization of the United nations); the silkworm pupa also contains trace elements such as potassium, sodium, calcium, magnesium, iron, copper, manganese, zinc, phosphorus, selenium and the like, vitamins A, E, B1, B2, carotene and the like; the silkworm pupa is rich in unsaturated fatty acid, about 72.5% of total fat, and is a high-quality animal protein without saturated fat in safety belt.

The invention aims to solve the problem that the old people have insufficient protein in vivo due to the insufficient recovery and utilization of cell protein in vivo caused by the aging of the body, realize the in vitro digestion by using a biological enzyme engineering technology, provide a protein product which can be directly absorbed through small intestines without digestion, solve the problem of protein imbalance of the old people, wherein the most important substance for human health is protein, immune cells are all composed of protein, and no protein exists, so the old people have no life.

The invention content is as follows:

the invention aims to provide a protein food which is digested in vitro for the old, and solves the problem of pain point of protein nutrient imbalance caused by incomplete digestion of protein due to aging of the old. Another manifestation of aging in the elderly is: the metabolism is slowed down, the digestion function is reduced, in particular, various hormones in the body are reduced, the hormones are important substances which are generated by the human body and used for regulating the normal activities of the body, the hormone secretion in the body is out of balance and must be supplemented by external sources from the aging point, the basis of the substances is protein, so the development of the digestion-free silkworm pupa protein product is significant for the health of old people, and the product is the only food which does not contain cholesterol in animal protein.

The preparation process comprises the following steps:

firstly, washing silkworm chrysalis raw materials, then crushing the cleaned silkworm chrysalis raw materials by using water in a ratio of 1 to 8-10 times, and thinning silkworm chrysalis materials;

secondly, sterilizing and modifying the refined silkworm chrysalis feed liquid, and instantaneously treating the silkworm chrysalis feed liquid at 125-130 ℃ for 10-16 seconds;

thirdly, the silkworm chrysalis feed liquid after sterilization and protein denaturation is cooled to 54-56 ℃;

fourthly, adding 0.3 to 0.5 percent of compound protease into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, carrying out deep enzymolysis for 36 to 72 hours, meeting the product standard requirement below 500 daltons, and not adjusting the pH value in the whole enzymolysis process;

fifthly, centrifugally separating the feed liquid after the enzymolysis is finished, and removing insoluble substances to obtain the digestion-free silkworm pupa protein feed liquid;

sixthly, sterilizing and inactivating enzyme in the step four, and keeping the temperature of the feed liquid at 90-95 ℃ for 15-25 seconds by a sterilizer to achieve the aim of killing protease;

seventhly, concentrating the solid material content of the materials to be more than 40 percent by using a double-effect evaporator respectively, and performing spray drying to obtain a digestion-free silkworm chrysalis protein powder product;

and sixthly, mixing the liquid materials respectively sterilized and inactivated by the step sixthly according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free silkworm chrysalis protein liquid product.

The invention has the following advantages and positive effects:

1. in order to solve the problem that the old people have insufficient protein due to the insufficient recycling of cell protein dropped from intestinal tracts in vivo caused by the aging of organisms, the enzyme engineering of the biotechnology is utilized to upgrade the traditional soybean protein product into a coffee indigestion-free protein product. The product after upgrading has the function of being absorbed without digestion, and can be used for the old with hypofunction of digestion, the people in a sick state and the people who can eliminate fatigue and recover physical performance as soon as possible. In addition, another manifestation of aging in the elderly is: metabolism slows down, digestion function reduces, especially various hormone in vivo reduces, hormone is that the human body produces, the basic material is protein, silkworm pupa protein is the only animal protein that does not contain cholesterol, the food chain requirement of silkworm pupa is very clean, so the development of the protein of the digestion-free silkworm pupa provides safer digestion-free protein product for the old.

2. Provides a protein nutritious food which can not be digested for patients. The research shows that: when a patient is ill, the patient is emaciated, the body is in a stop state due to the fact that the patient has good and multiple functions in the ill, if the patient does not have a stomach and is unwilling to eat, the digestion function is stopped, the body is consumed every moment, the body can only be used for meeting the needs of the body without supplementing the protein, and the patient is ill, the protein of the digestion-free silkworm chrysalis can be rapidly supplemented to the protein nutrient, so that the rehabilitation of the patient is greatly facilitated.

3. Provides a small molecule dipeptide and tripeptide protein product which is really free from digestion in the market. The small molecular dipeptide and tripeptide are the components of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering the body. American shake video: the whole process of killing cancer cells by immune cells in a human body is also realized by the principle of the existing immunotherapy that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells can kill cancer cells, the cancer incidence rate of the old is high, the body protein is insufficient, the most direct influence is the reduction of the immune cells, and the opportunity of killing the cancer cells is also reduced, so that: proliferating human immune cells is the most effective method for preventing and treating cancer.

There are only two ways for humans to obtain peptides:

one, eating small molecular dipeptide and tripeptide with molecular weight of 180 Da-500 Da.

Secondly, the peptide product is injected into the body through injection. Insulin, for example, is the 51 peptide, which saves countless lives of diabetics worldwide.

The specific implementation mode is as follows:

example 1:

weighing 500kg of silkworm chrysalis, washing, crushing by using water in an amount which is 1 to 8 to 10 times that of the silkworm chrysalis, and refining the silkworm chrysalis material;

secondly, sterilizing and modifying the refined silkworm chrysalis feed liquid, and instantaneously treating the silkworm chrysalis feed liquid at 120-130 ℃ for 3-6 seconds;

thirdly, the silkworm chrysalis feed liquid after sterilization and protein denaturation is cooled to 54-56 ℃;

adding 2kg of compound protease into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, and achieving the standard requirement of the product within 36 to 72 hours of deep enzymolysis time, wherein the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, centrifugally separating the feed liquid after the enzymolysis is finished to remove insoluble substances, and obtaining the digestion-free silkworm pupa protein feed liquid;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to kill protease;

seventhly, concentrating the solid material content of the material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free silkworm chrysalis protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated liquid material according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free silkworm pupa protein liquid product.

Example 2:

weighing 1000kg of silkworm chrysalis, washing, crushing by using water in a ratio of 1: 8-10 times, and refining the silkworm chrysalis material;

secondly, sterilizing and modifying the refined silkworm chrysalis feed liquid, and instantaneously treating the silkworm chrysalis feed liquid at 120-130 ℃ for 3-6 seconds;

thirdly, the silkworm chrysalis feed liquid after sterilization and protein denaturation is cooled to 54-56 ℃;

adding 400 g of compound protease into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, and enabling the deep enzymolysis time to reach the product standard requirement within 36 to 72 hours, wherein the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, centrifugally separating the feed liquid after the enzymolysis is finished to remove insoluble substances, and obtaining the digestion-free silkworm pupa protein feed liquid;

sterilizing the material liquid in the fifth step, keeping the temperature of the material liquid at 90-95 ℃ for 15-25 seconds by a sterilizer to achieve the aim of killing protease;

seventhly, concentrating the solid material content of the material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free silkworm chrysalis protein powder product;

and eighthly, preparing the sterilized and enzyme-deactivated liquid material according to the standard of the liquid product, and performing aseptic filling to obtain the active digestion-free silkworm chrysalis protein liquid product.