阅读说明：本技术 一种组合酶解法提取牡蛎肽的方法 (Method for extracting oyster peptide by combinatorial enzymolysis ) 是由 李立 郭红星 周尽学 于 2021-02-04 设计创作，主要内容包括：本发明公开了一种组合酶解法提取牡蛎肽的方法,步骤如下：一次汤储存装置中的一次汤泵入水解装置中,升温至设定灭菌温度121℃,并按照设定灭菌时长15～30分钟进行保持,然后降温至设定酶解温度50～55℃,加入配置液将一次汤PH值调节至中性,投入蛋白酶制剂进行水解,并保持至设定酶解时长,酶解结束后升温至设定灭酶温度,并保持至设定灭酶时长,形成灭酶后的水解液。通过将多种酶混合制成酶制剂粉料,使得多种酶共同作用,能够提高水解的效率,且酶制剂粉料中的酶激活剂能够加快酶的反应速率,进一步提高酶的水解效率,从而能够提高牡蛎肽的提取效率。(The invention discloses a method for extracting oyster peptide by a combinatorial enzymolysis method, which comprises the following steps: pumping the primary soup in the primary soup storage device into a hydrolysis device, heating to a set sterilization temperature of 121 ℃, keeping for 15-30 minutes according to the set sterilization time, then cooling to a set enzymolysis temperature of 50-55 ℃, adding a preparation solution to adjust the pH value of the primary soup to be neutral, adding a protease preparation for hydrolysis, keeping for the set enzymolysis time, heating to a set enzyme deactivation temperature after enzymolysis is finished, and keeping for the set enzyme deactivation time to form an enzyme deactivated hydrolysate. The enzyme preparation powder is prepared by mixing multiple enzymes, so that the efficiency of hydrolysis can be improved under the combined action of the multiple enzymes, the reaction rate of the enzymes can be accelerated by the enzyme activator in the enzyme preparation powder, the hydrolysis efficiency of the enzymes is further improved, and the extraction efficiency of the oyster peptides can be improved.)

1. A method for extracting oyster peptides by a combinatorial enzymolysis method is characterized by comprising the following steps: the method comprises the following steps:

the first step is as follows: cleaning the oysters according to a set cleaning duration to form cleaned oysters;

the second step is that: putting water into a cooking device, heating while adding weighed oysters, synchronously stirring, stopping heating after heating to a heat preservation range of 120-150 ℃, preserving heat for 2-3 hours according to a set heat preservation time length, stopping stirring after finishing heat preservation, and standing according to a set standing time length to form primary soup;

the third step: pumping the primary soup by using a pumping device and pumping the primary soup into a primary soup storage device;

the fourth step: pumping the primary soup in the primary soup storage device into a hydrolysis device, heating to a set sterilization temperature of 121 ℃, keeping for 15-30 minutes according to the set sterilization time, then cooling to a set enzymolysis temperature of 50-55 ℃, adding a preparation solution to adjust the pH value of the primary soup to be neutral, adding a protease preparation for hydrolysis, keeping for the set enzymolysis time, heating to a set enzyme deactivation temperature after enzymolysis is finished, and keeping for the set enzyme deactivation time to form an enzyme deactivated hydrolysate;

the fifth step: adding the hydrolysate subjected to enzyme deactivation into diatomite and/or active carbon, standing, filtering to a clear liquid storage device by using a filtering device, and performing circulating filtration on the clear liquid by using a circulating filtering device according to a set circulating filtration time length;

and a sixth step: concentrating the clear liquid after the circulating filtration to a set concentration to form a clarified concentrated solution;

the seventh step: and carrying out spray drying on the clarified concentrated solution according to the set air inlet temperature and the set air outlet temperature to form oyster peptide powder.

2. The method for extracting oyster peptides by a combinatorial enzymatic hydrolysis method according to claim 1, wherein the method comprises the following steps: the protease preparation in the fourth step comprises the following components: neutral protease alkaline protease flavourzyme 2:1: 1.

3. The method for extracting oyster peptides by a combinatorial enzymatic hydrolysis method according to claim 2, wherein the method comprises the following steps: the protease preparation comprises the following preparation steps:

1) mixing nanocrystalline cellulose in a solution with neutral pH to prepare a basic stock solution:

2) weighing neutral protease, alkaline protease and flavourzyme according to the ratio of 2:1:1, pouring the neutral protease, the alkaline protease and the flavourzyme into the basic stock solution, and oscillating and mixing for 5 minutes at normal temperature to obtain mixed suspension;

3) mixing the obtained mixed suspension with an enzyme activator, and shaking for 15 minutes;

4) sending the mixed solution obtained in the step 3) to a pressure spray tower for spray drying, and obtaining enzyme preparation powder.

4. The method for extracting oyster peptides by a combinatorial enzymatic hydrolysis method according to claim 3, wherein the method comprises the following steps: the enzyme activator in 3) is Ca²⁺And (3) solution.

Technical Field

The invention belongs to the technical field of oyster peptide extraction, and particularly relates to a method for extracting oyster peptide by a combinatorial enzymatic hydrolysis method.

Background

The oyster peptide is prepared by applying peptide molecular biotechnology to oyster processing and performing enzymolysis. The micromolecule oligopeptide formed by the preparation method completely reserves the original nutrients such as vitamins, trace elements, taurine and the like of the oysters, so that the oysters rich in nucleic acid can be absorbed by the human body more quickly than single amino acid or protein after being ingested by the human body, are absorbed by the human body more easily, have more important biological activity in the aspect of human metabolism, and can effectively improve the male serum testosterone level. Has higher biological value and more important physiological function than the common oyster products.

At present, protease is required to be added for hydrolysis in the process of extracting oyster peptides. However, in the prior art, single enzyme is added for hydrolysis during extraction of oyster peptides, so that the hydrolysis efficiency is low, and the extraction efficiency of the oyster peptides is influenced.

Disclosure of Invention

The invention aims to provide a method for extracting oyster peptides by a combinatorial enzymatic hydrolysis method, which aims to solve the problems that the oyster peptides are hydrolyzed by adding single enzyme during extraction, the hydrolysis efficiency is low, and the extraction efficiency of the oyster peptides is influenced.

In order to achieve the purpose, the invention provides the following technical scheme: a method for extracting oyster peptide by a combinatorial enzymolysis method comprises the following steps:

the first step is as follows: cleaning the oysters according to a set cleaning duration to form cleaned oysters;

the second step is that: putting water into a cooking device, heating while adding weighed oysters, synchronously stirring, stopping heating after heating to a heat preservation range of 120-150 ℃, preserving heat for 2-3 hours according to a set heat preservation time length, stopping stirring after finishing heat preservation, and standing according to a set standing time length to form primary soup;

the third step: pumping the primary soup by using a pumping device and pumping the primary soup into a primary soup storage device;

the fourth step: pumping the primary soup in the primary soup storage device into a hydrolysis device, heating to a set sterilization temperature of 121 ℃, keeping for 15-30 minutes according to the set sterilization time, then cooling to a set enzymolysis temperature of 50-55 ℃, adding a preparation solution to adjust the pH value of the primary soup to be neutral, adding a protease preparation for hydrolysis, keeping for the set enzymolysis time, heating to a set enzyme deactivation temperature after enzymolysis is finished, and keeping for the set enzyme deactivation time to form an enzyme deactivated hydrolysate;

the fifth step: adding the hydrolysate subjected to enzyme deactivation into diatomite and/or active carbon, standing, filtering to a clear liquid storage device by using a filtering device, and performing circulating filtration on the clear liquid by using a circulating filtering device according to a set circulating filtration time length;

and a sixth step: concentrating the clear liquid after the circulating filtration to a set concentration to form a clarified concentrated solution;

the seventh step: and carrying out spray drying on the clarified concentrated solution according to the set air inlet temperature and the set air outlet temperature to form oyster peptide powder.

Preferably, the composition of the protease preparation in the fourth step is: neutral protease alkaline protease flavourzyme 2:1: 1.

Preferably, the protease preparation is prepared by the following steps:

1) mixing nanocrystalline cellulose in a solution with neutral pH to prepare a basic stock solution:

2) weighing neutral protease, alkaline protease and flavourzyme according to the ratio of 2:1:1, pouring the neutral protease, the alkaline protease and the flavourzyme into the basic stock solution, and oscillating and mixing for 5 minutes at normal temperature to obtain mixed suspension;

3) mixing the obtained mixed suspension with an enzyme activator, and shaking for 15 minutes;

4) sending the mixed solution obtained in the step 3) to a pressure spray tower for spray drying, and obtaining enzyme preparation powder.

Preferably, the enzyme activator in 3) is Ca²⁺And (3) solution.

Compared with the prior art, the invention has the beneficial effects that: the enzyme preparation powder is prepared by mixing multiple enzymes, so that the efficiency of hydrolysis can be improved under the combined action of the multiple enzymes, the reaction rate of the enzymes can be accelerated by the enzyme activator in the enzyme preparation powder, the hydrolysis efficiency of the enzymes is further improved, and the extraction efficiency of the oyster peptides can be improved.

Detailed Description

A method for extracting oyster peptide by a combinatorial enzymolysis method comprises the following steps:

the first step is as follows: cleaning the oysters according to a set cleaning duration to form cleaned oysters;

the second step is that: putting water into a cooking device, heating while adding weighed oysters, synchronously stirring, stopping heating after heating to a heat preservation range of 120-150 ℃, preserving heat for 2-3 hours according to a set heat preservation time length, stopping stirring after finishing heat preservation, and standing according to a set standing time length to form primary soup;

the third step: pumping the primary soup by using a pumping device and pumping the primary soup into a primary soup storage device;

the fourth step: pumping the primary soup in the primary soup storage device into a hydrolysis device, heating to a set sterilization temperature of 121 ℃, keeping for 15-30 minutes according to the set sterilization time, then cooling to a set enzymolysis temperature of 50-55 ℃, adding a preparation solution to adjust the pH value of the primary soup to be neutral, adding a protease preparation for hydrolysis, keeping for the set enzymolysis time, heating to a set enzyme deactivation temperature after enzymolysis is finished, and keeping for the set enzyme deactivation time to form an enzyme deactivated hydrolysate;

the fifth step: adding the hydrolysate subjected to enzyme deactivation into diatomite and/or active carbon, standing, filtering to a clear liquid storage device by using a filtering device, and performing circulating filtration on the clear liquid by using a circulating filtering device according to a set circulating filtration time length;

and a sixth step: concentrating the clear liquid after the circulating filtration to a set concentration to form a clarified concentrated solution;

the seventh step: and carrying out spray drying on the clarified concentrated solution according to the set air inlet temperature and the set air outlet temperature to form oyster peptide powder.

The protease preparation in the fourth step comprises the following components: neutral protease alkaline protease flavourzyme 2:1: 1.

The protease preparation comprises the following preparation steps:

1) mixing nanocrystalline cellulose in a solution with neutral pH to prepare a basic stock solution:

2) weighing neutral protease, alkaline protease and flavourzyme according to the ratio of 2:1:1, pouring the neutral protease, the alkaline protease and the flavourzyme into the basic stock solution, and oscillating and mixing for 5 minutes at normal temperature to obtain mixed suspension;

3) mixing the obtained mixed suspension with an enzyme activator, and shaking for 15 minutes;

4) sending the mixed solution obtained in the step 3) to a pressure spray tower for spray drying, and obtaining enzyme preparation powder.

The enzyme activator in 3) is Ca²⁺And (3) solution.