阅读说明：本技术 从甜菜糖蜜中提取天然甜菜碱的方法 (Method for extracting natural betaine from beet molasses ) 是由 邹传军 于 2020-12-08 设计创作，主要内容包括：本发明公开了一种从甜菜糖蜜中提取天然甜菜碱的方法,该方法首先将含有甜菜碱的甜菜糖蜜经过色谱分离,得到含有甜菜碱、果糖、葡萄糖的混合物；将上述混合物进行发酵处理消耗其中的果糖和葡萄糖；将发酵物进行浓缩、过滤、纯化,得到甜菜碱。本发明的方法将果糖、葡萄糖作为碳源发酵转化为乙醇,技术简单,得到的产物-乙醇,沸点低,在甜菜碱的浓缩过程中,可以很容易蒸发除去。在发酵过程中,甜菜碱不参与任何反应,因此,甜菜碱的得率为100％,而还原糖在发酵过程中消耗率接近100％,由于色谱分离的产物,甜菜碱+还原糖的总量超过98％,因此,得到的甜菜碱的纯度可以达到98％以上。该技术,工艺简单,技术成熟,投资小,效果好。(The invention discloses a method for extracting natural betaine from beet molasses, which comprises the steps of firstly, carrying out chromatographic separation on the beet molasses containing the betaine to obtain a mixture containing the betaine, fructose and glucose; fermenting the mixture to consume fructose and glucose in the mixture; and concentrating, filtering and purifying the fermentation product to obtain the betaine. The method of the invention converts fructose and glucose into ethanol by fermentation as carbon sources, has simple technology, and the obtained product-ethanol has low boiling point and can be easily evaporated and removed in the concentration process of betaine. In the fermentation process, betaine does not participate in any reaction, so the yield of the betaine is 100%, the consumption rate of reducing sugar in the fermentation process is close to 100%, and the total amount of the betaine and the reducing sugar exceeds 98% due to chromatographic separation products, so the purity of the obtained betaine can reach more than 98%. The technology has the advantages of simple process, mature technology, small investment and good effect.)

1. A method for extracting natural betaine from beet molasses is characterized in that: the method comprises the following steps:

1) firstly, carrying out chromatographic separation on beet molasses containing betaine to obtain a mixture containing betaine, fructose and glucose;

2) fermenting the mixture to consume fructose and glucose in the mixture to obtain a fermented product;

3) distilling, filtering and purifying the fermented product to obtain the betaine.

2. The method for extracting natural betaine from beet molasses as claimed in claim 1, wherein: in the step 1), the betaine content in the beet molasses is 3-8%.

3. The method for extracting natural betaine from beet molasses as claimed in claim 1, wherein: in the step 1), the content of betaine in the mixture is 10-80%.

4. The method for extracting natural betaine from beet molasses as claimed in claim 3, wherein: in the step 1), the content of betaine in the mixture is 50-80%.

5. The method for extracting natural betaine from beet molasses as claimed in claim 3, wherein: in the step 2), the fermentation treatment is yeast inoculation fermentation.

6. The method for extracting natural betaine from beet molasses as claimed in claim 1, wherein: in the step 3), the purity of the betaine is 90-99%.

Technical Field

The invention relates to the field of betaine production, and particularly relates to a method for extracting natural betaine from beet molasses.

Background

Betaine is an alkaloid which is very soluble in water, and molasses of the betaine is a main source of the betaine, so the betaine is named. Betaine is ubiquitous in animals and plants, is an intermediate product of animal metabolism, and plays an important role in the metabolism of nutrients. Is a secondary product of metabolism, is a very important osmotic adjusting substance and is very important for enhancing the stress resistance of plants, such as salt and alkali resistance and drought tolerance. People find that one component in the beet can play a good cleaning role, does not hurt the skin, is very safe, is named as betaine, and is used for cleaning skin care products safely and effectively in recent times.

The extraction of natural betaine from beet molasses generally uses chromatographic separation, such as the method disclosed in chinese patent CN 201510999995.1. Since betaine has a molecular weight of 117 and fructose and glucose have a molecular weight of 180, betaine cannot be separated from fructose and glucose by a membrane filtration technique or a chromatographic separation process, and betaine, fructose and glucose have high solubility, cannot be separated by crystallization, and are difficult to separate by a fractionation technique or the like, so that fructose and glucose affect purification of betaine. To solve this problem, chinese patent CN201280064269.5 discloses a method of subjecting fructose and glucose to special enzyme treatment to obtain polymers of fructose and glucose with molecular weight of more than 342, so that the polymers can be separated from betaine by membrane filtration and chromatographic separation. The defects of the technology are as follows: the fructose and the glucose are treated by special enzyme, the operation cost is high, secondary separation is needed, and the process is complex.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a method for extracting natural betaine from beet molasses, which is simple to operate, strong in practicability and low in cost.

In order to achieve the purpose, the invention relates to a method for extracting natural betaine from beet molasses, which comprises the following steps:

1) firstly, carrying out chromatographic separation on beet molasses containing betaine to obtain a mixture containing betaine, fructose and glucose;

2) fermenting the mixture to consume fructose and glucose in the mixture to obtain a fermented product;

(fermentation process can be carried out in various ways, fructose and glucose are used as carbon sources to obtain various fermentation products, typically yeast is used to convert fructose and glucose into ethanol, ethanol can be removed by evaporation in the concentration process of betaine, and yeast can be removed by filtration);

an anaerobic glycolysis performed by an organism to produce ethanol and carbon dioxide from a sugar or polysaccharide, of the formula:

C₆H₁₂O₆——2CH₃CH₂OH+2CO₂

3) and concentrating, filtering and purifying the fermentation product to obtain the betaine.

Further, in the step 1), the content of betaine in the beet molasses is 3-8%.

Further, in the step 1), the content of betaine in the mixture is 10-80%.

Further, in the step 1), the content of betaine in the mixture is 50-80%.

Still further, in the step 2), the fermentation treatment is yeast fermentation.

Still further, in the step 3), the purity of the betaine is 90-99%.

The invention has the beneficial effects that:

the method of the invention uses fructose and glucose as carbon sources to ferment and transfer the fructose and the glucose into the ethanol, has simple technology, and the obtained product, namely the ethanol, has low boiling point and can be easily evaporated and removed in the concentration process of the betaine. In the fermentation process, betaine does not participate in any reaction, so the yield of the betaine is 100%, the consumption rate of reducing sugar in the fermentation process is close to 100%, and the total amount of the betaine and the reducing sugar exceeds 98% due to chromatographic separation products, so the purity of the obtained betaine can reach more than 98%. The technology has the advantages of simple process, mature technology, small investment and good effect.

Detailed Description

The present invention is described in further detail below with reference to specific examples so as to be understood by those skilled in the art.

Example 1

A method for extracting natural betaine 1 from beet molasses comprises the following steps:

1) firstly, carrying out chromatographic separation on beet molasses (the content of betaine is 3%) to obtain a mixture containing betaine, fructose and glucose; wherein the mixture comprises the following components:

composition (I)	Ratio of
		Betaine	65％
Fructose, glucose	34.2％
		Others	0.8％

2) Fermenting the mixture with yeast to consume fructose and glucose therein to obtain a fermented product;

3) concentrating, filtering and purifying the fermented product to obtain the betaine with the purity of 99.2%.

Example 2

A method for extracting natural betaine 2 from beet molasses comprises the following steps:

1) firstly, carrying out chromatographic separation on beet molasses (the content of betaine is 5%) to obtain a mixture containing betaine, fructose and glucose; wherein the mixture comprises the following components:

composition (I)	Ratio of
		Betaine	60％
Fructose, glucose	38.5％
		Others	1.5％

2) Fermenting the mixture with yeast to consume fructose and glucose therein to obtain a fermented product;

3) concentrating, filtering and purifying the fermented product to obtain the betaine with the purity of 98.5%.

Example 3

A method for extracting natural betaine 3 from beet molasses comprises the following steps:

1) firstly, carrying out chromatographic separation on beet molasses (the content of betaine is 8%) to obtain a mixture containing betaine, fructose and glucose; wherein the mixture comprises the following components:

composition (I)	Ratio of
		Betaine	80％
Fructose, glucose	19.5％
		Others	0.5％

2) Fermenting the mixture with yeast to consume fructose and glucose therein to obtain a fermented product;

3) concentrating, filtering and purifying the fermented product to obtain the betaine with the purity of 99.5%.

Other parts not described in detail are prior art. Although the above examples are intended to describe the present invention in detail, it is only a part of the examples, not all examples, and one can obtain other examples without inventive step, for example, by using fermentation method to convert fructose and glucose into other products, which are all within the scope of the present invention.