阅读说明：本技术 一种大豆低聚肽的生产工艺 (Production process of soybean oligopeptide ) 是由 邹鹏 陈雷 匙金祥 于 2020-08-12 设计创作，主要内容包括：本发明公开了一种大豆低聚肽的生产工艺,涉及低聚肽生产技术领域。本发明包括以下步骤：步骤一：在大豆分离蛋白中加入纯水搅拌至溶解,配得大豆分离蛋白溶液；步骤二：将大豆分离蛋白溶液输送至酶解罐中持续搅拌,加入蛋白酶进行酶解后得到酶解液；步骤三：调节酶解液PH值至等电点,未酶解的大豆分离蛋白过滤分离得到浊液和清液；步骤四：将清液脱盐浓缩,脱苦脱色、澄清后得到浓缩肽液；步骤五：将浓缩肽液进行压力喷雾后获得大豆低聚肽粉末。本发明通过使用陶瓷管式膜和纳滤设备对大豆低聚肽粉末的生产工艺进行改进,缩短工艺步骤,提高生产过程稳定性,通过多种酶的相互补充,提升大豆分离蛋白酶解的效果,使产品更容易被吸收。(The invention discloses a production process of soybean oligopeptide, and relates to the technical field of oligopeptide production. The invention comprises the following steps: the method comprises the following steps: adding pure water into the isolated soy protein, stirring until the pure water is dissolved, and preparing a isolated soy protein solution; step two: conveying the soy protein isolate solution into an enzymolysis tank, continuously stirring, adding protease for enzymolysis to obtain an enzymolysis solution; step three: adjusting the pH value of the enzymolysis liquid to an isoelectric point, and filtering and separating the soybean protein isolate which is not subjected to enzymolysis to obtain turbid liquid and clear liquid; step four: desalting and concentrating the clear liquid, debitterizing, decolorizing and clarifying to obtain concentrated peptide liquid; step five: and (3) carrying out pressure spraying on the concentrated peptide solution to obtain soybean oligopeptide powder. According to the invention, the production process of the soybean oligopeptide powder is improved by using the ceramic tubular membrane and the nanofiltration equipment, the process steps are shortened, the stability of the production process is improved, and the enzymolysis effect of the soybean protein isolate is improved by mutual supplement of various enzymes, so that the product is easier to absorb.)

1. A production process of soybean oligopeptide is characterized by comprising the following steps: the method comprises the following steps:

the method comprises the following steps: adding pure water into the isolated soy protein, stirring until the pure water is dissolved, and preparing a isolated soy protein solution;

step two: conveying the soybean protein isolate solution into an enzymolysis tank, continuously stirring, sterilizing at high temperature, adding protease for enzymolysis, and inactivating at high temperature to obtain an enzymolysis solution;

step three: cooling the enzymolysis liquid to 70 ℃, adjusting the pH value of the enzymolysis liquid to an isoelectric point, performing acid precipitation on the soybean protein isolate which is not subjected to enzymolysis, filtering and separating to obtain turbid liquid and clear liquid, and drying and screening the turbid liquid to obtain feed protein;

step four: desalting and concentrating the clear liquid, performing high-temperature sterilization, debitterizing, decoloring and clarifying to obtain a concentrated peptide liquid;

step five: and (3) carrying out pressure spraying on the concentrated peptide solution to obtain soybean oligopeptide powder.

2. The process for producing soybean oligopeptide according to claim 1, wherein in the first step, the ratio of the isolated soybean protein to the pure water is as follows: 1: 25; the pure water in the first step is first-level RO produced water, and the conductivity index is less than or equal to 20 mu s/cm.

3. The process for producing soybean oligopeptide according to claim 1, wherein in the second step, the temperature for the first high-temperature inactivation is 90 ℃ and the duration is 30 min; the temperature of the second high temperature inactivation is 90 ℃, and the duration time is 30 min;

the method comprises the following steps of (1) reducing the temperature of a soybean protein isolate solution subjected to high-temperature sterilization to 55 ℃ and keeping the temperature, adding alkaline protease, adjusting the pH value of the solution to 9.0, adding the alkaline protease, carrying out primary reaction for 60min, adjusting the pH value to 7.0, adding the neutral protease, carrying out primary reaction for 10min, adding the flavor enzyme, keeping the temperature of the solution at 55 ℃, and continuously stirring for 5h, wherein the alkaline protease, the neutral protease and the flavor enzyme are mixed with the soybean protein isolate solution to be reacted according to the weight ratio: 2:1:1:4000.

4. The process for producing soybean oligopeptide according to claim 1, wherein in the third step, the pH value of the enzymatic hydrolysate is adjusted by adding hydrochloric acid; and filtering and separating the acid-precipitated enzymolysis liquid by using a ceramic tubular membrane, wherein the pore size of the ceramic tubular membrane is 50 nm.

5. The process for producing soybean oligopeptide according to claim 1, wherein in the fourth step, the clear solution is desalted and concentrated by nanofiltration, the temperature of high-temperature sterilization is 90 ℃, the duration is 60min, activated carbon powder is added, stirring and filtering are carried out, the desalted and concentrated clear solution is debittered and decolored, wherein the adding amount of the activated carbon and the unsaturated degree of the desalted and concentrated clear solution are added according to the ratio of 1: 100.

6. The process for producing soybean oligopeptide according to claim 1, wherein the step five further comprises the following steps:

step six: and (3) carrying out index detection on the soybean oligopeptide powder, weighing the qualified soybean oligopeptide powder, packaging and warehousing.

Technical Field

The invention belongs to the technical field of oligopeptide production, and particularly relates to a production process of soybean oligopeptide.

Background

Oligopeptide refers to peptide with 2-10 amino acids, which is easy to be absorbed by human body due to small molecular weight, and soybean is a plant protein with wide source.

At present, in the production of soybean oligopeptide, low-temperature defatted soybean meal is mostly selected and subjected to more than two times of acid extraction, solid-liquid separation, alkali liquor regulation and other complex processes to obtain slurry for enzymolysis, and the method has high requirements on selected raw materials and is complex and unstable in treatment; in the production process, tap water or drinking water is mostly used as process water, the hardness is high, the treatment of subsequent working sections is influenced, the taste of the final product is influenced, protease for protein hydrolysis is single, the enzymolysis time is long, the hydrolysis degree of protein is low, the taste of the product is poor, and the yield is low.

Disclosure of Invention

The invention aims to provide a production process of soybean oligopeptide, and solves the problems that the existing soybean oligopeptide product process is complex and difficult to control, the uniformity of product quality is difficult to ensure, and the product taste is poor.

In order to solve the technical problems, the invention is realized by the following technical scheme:

the invention relates to a production process of soybean oligopeptide, which comprises the following steps:

the method comprises the following steps: adding pure water into the selected soybean protein isolate, stirring until the pure water is dissolved, and preparing a soybean protein isolate solution;

step two: conveying the soybean protein isolate solution into an enzymolysis tank, continuously stirring, sterilizing at high temperature, adding protease for enzymolysis, and inactivating at high temperature to obtain an enzymolysis solution;

step three: cooling the enzymolysis liquid to 70 ℃, adjusting the pH value of the enzymolysis liquid to an isoelectric point, performing acid precipitation on the soybean protein isolate which is not subjected to enzymolysis, filtering and separating to obtain turbid liquid and clear liquid, and drying and screening the turbid liquid to obtain feed protein;

step four: desalting and concentrating the clear liquid, performing high-temperature sterilization, debitterizing, decoloring and clarifying to obtain a concentrated peptide liquid;

step five: and (3) carrying out pressure spraying on the concentrated peptide solution to obtain soybean oligopeptide powder.

Preferably, in the first step, the ratio of the soy protein isolate to the pure water is as follows by weight: 1: 25; the pure water in the first step is first-level RO produced water, the conductivity index is less than or equal to 20 mu s/cm, the hardness and the conductivity of the tap water are reduced through membrane separation, and the quality of the soybean protein isolate solution is improved, so that the stability of the product in production is improved.

Preferably, in the second step, the temperature for the first high-temperature inactivation is 90 ℃, and the duration time is 30 min; the temperature of the second high temperature inactivation is 90 ℃, and the duration time is 30 min;

the method comprises the following steps of (1) reducing the temperature of a soybean protein isolate solution subjected to high-temperature sterilization to 55 ℃, keeping the temperature, adjusting the pH value of the solution to 9.0, adding alkaline protease, performing an initial reaction for 60min, adjusting the pH value to 7.0, adding neutral protease, performing an initial reaction for 10min, adding flavor enzyme, keeping the temperature of the solution at 55 ℃, and continuously stirring for 5h, wherein the alkaline protease, the neutral protease and the flavor enzyme are mixed with the soybean protein isolate solution to be reacted according to the weight ratio: 2:1:1:4000, and the three proteases are mutually matched and supplemented, so that the enzymolysis rate and the enzymolysis rate of the soybean protein isolate solution are further improved, the production efficiency is improved, and the product taste is improved.

Preferably, in the third step, the pH value of the enzymolysis liquid is adjusted by adding hydrochloric acid; the enzymolysis liquid after acid precipitation is filtered and separated by a ceramic tubular membrane, the pore size of the ceramic tubular membrane is 50nm, the product is filtered by the ceramic tubular membrane, the ceramic tubular membrane can be repeatedly used, a filter aid does not need to be added in production, the pollution to the environment is avoided, and the product quality is improved.

Preferably, in the fourth step, the clear liquid is desalted and concentrated through nanofiltration, the temperature of high-temperature sterilization is 90 ℃, the duration time is 60min, activated carbon powder is added, stirring and filtering are carried out, the desalted and concentrated clear liquid is debittered and decolorized, wherein the adding amount of the activated carbon and the unsaturation degree of the desalted and concentrated clear liquid are added according to the proportion of 1:100, desalting and concentration are carried out through nanofiltration equipment, the production process steps are reduced, the pollution problem caused by excessive processes is avoided, and the production stability of the product is improved.

Preferably, the step five further comprises the following steps:

step six: and (3) carrying out index detection on the soybean oligopeptide powder, weighing the qualified soybean oligopeptide powder, packaging and warehousing.

The invention has the following beneficial effects:

according to the invention, the production process of the soybean oligopeptide powder is improved by using the ceramic tubular membrane and the nanofiltration equipment, so that the process steps are shortened, the stability of the production process is improved, the product quality is improved, the enzymolysis effect of soybean protein is improved by mutual supplement of various enzymes, the product is easier to absorb, and the taste of the product is improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a flow chart of a process for producing soybean oligopeptide.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the present invention relates to a process for producing soybean oligopeptide, comprising the following steps:

the method comprises the following steps: adding pure water into the selected soybean protein isolate, stirring until the pure water is dissolved, and preparing a soybean protein isolate solution;

step two: conveying the soy protein isolate solution into an enzymolysis tank, continuously stirring, sterilizing at high temperature, adding protease for enzymolysis, and inactivating at high temperature to obtain an enzymolysis solution;

step three: cooling the enzymolysis solution to 70 ℃, adjusting the pH value of the enzymolysis solution to an isoelectric point, performing acid precipitation on the soybean protein isolate which is not subjected to enzymolysis, filtering and separating to obtain a turbid solution and a clear solution, and drying and screening the turbid solution to obtain the feed protein;

step four: desalting and concentrating the clear liquid, sterilizing at high temperature, debitterizing, decolorizing, and clarifying to obtain concentrated peptide solution;

step five: and (3) carrying out pressure spraying on the concentrated peptide solution to obtain soybean oligopeptide powder.

Preferably, in the first step, the ratio of the soy protein isolate to the pure water is as follows by weight: 1: 25; the pure water in the first step is first-level RO water, the conductivity index is less than or equal to 20 mu s/cm, the hardness and the conductivity of the tap water are reduced through membrane separation, and the quality of the soybean protein isolate solution is improved, so that the stability of the product in production is improved.

Preferably, in the second step, the temperature of the first high-temperature inactivation is 90 ℃, and the duration time is 30 min; the temperature of the second high temperature inactivation is 90 ℃, and the duration time is 30 min;

the method comprises the following steps of (1) reducing the temperature of a soybean protein isolate solution subjected to high-temperature sterilization to 55 ℃, keeping the temperature, adjusting the pH value of the solution to 9.0, adding alkaline protease, carrying out primary reaction for 60min, adjusting the pH value to 7.0, adding neutral protease, carrying out primary reaction for 10min, adding flavor enzyme, keeping the temperature of the solution at 55 ℃, and continuously stirring for 5h, wherein the alkaline protease, the neutral protease and the flavor enzyme are mixed with the soybean protein isolate solution to be reacted according to the weight ratio: 2:1:1:4000, and the three proteases are mutually matched and supplemented, so that the enzymolysis rate and the enzymolysis rate of the soybean protein isolate solution are further improved, the production efficiency is improved, and the product taste is improved.

Preferably, in the third step, the pH value of the enzymolysis liquid is adjusted by adding hydrochloric acid; the enzymolysis liquid after acid precipitation is filtered and separated by a ceramic tubular membrane, the pore size of the ceramic tubular membrane is 50nm, the product is filtered by the ceramic tubular membrane, the ceramic tubular membrane can be repeatedly used, a filter aid does not need to be added in production, the pollution to the environment is avoided, and the product quality is improved.

Preferably, in the fourth step, the clear liquid is desalted and concentrated through nanofiltration, the temperature of high-temperature sterilization is 90 ℃, the duration time is 60min, activated carbon powder is added, stirring and filtering are carried out, the desalted and concentrated clear liquid is debittered and decolorized, wherein the adding amount of the activated carbon and the unsaturation degree of the desalted and concentrated clear liquid are added according to the proportion of 1:100, desalting and concentration are carried out through nanofiltration equipment, the production process steps are reduced, the pollution problem caused by excessive processes is avoided, and the production stability of the product is improved.

Preferably, step five further comprises the steps of:

step six: and (4) performing index detection on the soybean oligopeptide powder, weighing the qualified soybean oligopeptide powder, packaging and warehousing.

The first embodiment is as follows: mouse weight bearing swimming test

The soybean oligopeptide product produced by the production process of the soybean oligopeptide is used for carrying out a mouse weight-bearing swimming test, 60 healthy male white mice with the same age of day are selected for the mice under experimental conditions, four groups of white mice are respectively fed with distilled water, 2% glucose solution, low-dose oligopeptide and high-dose oligopeptide for 30min, wherein the feeding amount of the low-dose oligopeptide product is 3g per mouse, the feeding amount of the high-dose oligopeptide product is 10g per mouse, after the mice are fed for 30min, weight-bearing lead skins which are 5% of the weight of the mice are respectively bound at the tail root of each white mouse, the white mice are put into water, the swimming time of the white mice is measured, and the average swimming time of each group of white mice is counted, and the test results are shown in the following table:

table one: white mouse load swimming test data table

Group of	Animal number (only)	Average swimming time (S)
			High dose group	15	657
Low dose group	15	522
			2% glucose solution	15	441
Distilled water	15	357

As can be seen from the data in the table, the time for the mice of the soybean oligopeptide group to stay for swimming is obviously longer than that of the glucose group, the time for the mice of the high-dose group to stay for swimming is longer than that of the mice of the low-dose group in the soybean oligopeptide group, and the time for the mice of the distilled water group is shortest, so that the soybean oligopeptide has the effects of quickly restoring the physical strength of animals, improving the endurance and resisting fatigue.

Example two:

the soybean oligopeptide product produced by the production process of the soybean oligopeptide is taken for sample detection, and the samples are respectively subjected to microbial detection and physical and chemical detection, wherein the detection results are shown in the following table:

table two: microorganism detection meter

Detecting items	Unit of	Detection method	The result of the detection
				Total number of colonies	CFU/g	GB 4789.2-2016	＜10
Coliform group bacteria	MPN/g	First method of GB 4789.3-2016	＜0.3
				Mould fungus	CFU/g	First method of GB 4789.15-2016	＜10
Yeast	CFU/g	First method of GB 4789.15-2016	＜10
				Staphylococcus aureus	/25g	First method of GB 4789.10-2016	ND
Salmonella	/25g	GB 4789.4-2016	ND

Table three: physical and chemical detection table

The nitrogen conversion coefficient to protein was 6.25, and ND means no detection.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.