High performance liquid chromatography analysis method for polypeptide mixture
阅读说明：本技术 多肽混合物高效液相色谱分析方法 (High performance liquid chromatography analysis method for polypeptide mixture ) 是由 唐洋明 何刚 李国弢 陶安进 袁建成 于 2015-04-28 设计创作，主要内容包括：本发明涉及一种多肽混合物高效液相色谱分析方法,具体而言,该方法包括如下步骤,步骤(1)配置待测醋酸格拉替雷溶液；步骤(2)以反相液色谱法,对待测样本进行梯度洗脱,步骤(3)检测分析共聚物各组分对应峰面积,通过与对照品比对,确定待测样本各组分含量范围是否合格。(The invention relates to a high performance liquid chromatography analysis method for a polypeptide mixture, which specifically comprises the following steps of (1) preparing a glatiramer acetate solution to be detected; and (2) carrying out gradient elution on the sample to be detected by using a reversed phase liquid chromatography, detecting and analyzing corresponding peak areas of all components of the copolymer in the step (3), and comparing the peak areas with a reference substance to determine whether the content range of all the components of the sample to be detected is qualified.)
1. A method for carrying out linear or step gradient elution on glatiramer acetate by adopting a high performance liquid chromatography and analyzing whether the content of each component of the copolymer is qualified or not is characterized by comprising the following steps,
preparing a glatiramer acetate solution to be detected;
step (2) using reversed phase liquid chromatography to carry out gradient elution on a sample to be detected,
and (3) detecting and analyzing corresponding peak areas of all components of the copolymer, and comparing the peak areas with a reference substance to determine whether the content range of each component of the sample to be detected is qualified.
2. The method of claim 1,
the chromatographic conditions of the reversed phase liquid chromatography adopted in the step (2) are as follows:
a chromatographic column taking C18, C12, C8 and C4 bonded silica gel particles as fillers is adopted, and the particle size of the chromatographic column filler particles is 1.7-10 mu m;
acetonitrile is used as a mobile phase A;
taking a solution containing 30-80mM ammonium sulfate as a mobile phase B, and adjusting the pH value to 2-3 by using a phosphoric acid solution;
or 0.1% trifluoroacetic acid solution is used as mobile phase B;
the sample amount is 5-50uL, the sample concentration is 1-20mg/ml,
the flow rate is 0.5-1.5mL/min, and the elution time is 50-250 min;
the elution gradient was:
the total elution time is equally divided into N (N is more than or equal to 2 and less than or equal to 20) steps, gradient elution is sequentially carried out, the proportion of the mobile phase A in the gradient of the first N-1 step is gradually increased from 5 percent to 40 percent, the proportion of the mobile phase B is gradually decreased from 95 percent to 60 percent, the proportion of the mobile phase A in the Nth step is 5 percent, and the proportion of the mobile phase B in the Nth step is 95 percent;
preferably 5. ltoreq. N.ltoreq.15, more preferably 8. ltoreq. N.ltoreq.12, most preferably, N.ltoreq.10;
the column temperature is 25-50 ℃.
3. The method according to any one of claims 1 to 2,
the detection analysis in the step (3) adopts an ultraviolet detector to detect the wavelength of 260-280 nm;
when a fluorescence detector is used, the excitation wavelength is 230nm and the emission wavelength is 300 nm.
Technical Field
The invention belongs to the field of biological medicine, and particularly relates to a high performance liquid chromatography analysis method for a polypeptide mixture.
Background
Glatiramer acetate (abbreviated as GA) is a synthetic polypeptide mixture (relative molecular weight: 4700 and 11000 daltons) which is a random polymer composed of four amino acids of L-alanine, L-glutamic acid, L-tyrosine and L-lysine, and has a length of 45-100 amino acids. The molar ratio of each amino acid is about 0.392-0.462, 0.129-0.153, 0.086-0.100, 0.300-0.374. The medicine is developed and manufactured by Tibet pharmaceutical factory terra tile pharmacy (TEVA), and has trade name ofFDA approval for the treatment of multiple sclerosis was obtained in 1996 in the United states and is currently availableThere are two kinds of products, water injection and freeze dried powder injection, which are used for subcutaneous injection.
Glatiramer acetate is a copolymer with strong continuity, and the structure of the copolymer is shown as the following formula:
(Glu，Ala，Lys，Tyr)xxCH3COOH
(C5H9NO4.C3H7NO2.C6H14N2O2.C9H11NO3)x.
xC2H4O2
CAS-147245-92-9
to imitate the pharmaceutical manufacturer, the compositional differences of the imitation glatiramer acetate and the control formulation glatiramer acetate can only be examined based on some inherent properties of the drug.
Based on the charge, polarity and non-polar conditions of the product in different buffer solutions, the invention adopts anion exchange chromatography, cation exchange chromatography and reversed phase chromatography to carry out step gradient elution to compare and analyze the difference between a trial sample and a commercial preparation (a reference product).
Based on that glatiramer acetate is a copolymer with strong continuity, the components are difficult to be clarified by a separation method. The existing analysis method is based on the molecular weight difference among components and uses a size exclusion method to carry out simple separation analysis (a single peak is divided into a plurality of components to be analyzed after being collected), so that the literature method is complicated. It is highly desirable to develop analytical methods that effectively separate the components of glatiramer acetate.
Disclosure of Invention
The invention firstly relates to a method for carrying out linear or step gradient elution on glatiramer acetate by adopting a high performance liquid chromatography and analyzing whether the content of each component of the copolymer is qualified or not.
The method comprises the following steps of,
preparing a glatiramer acetate solution to be detected;
step (2) using anion exchange liquid chromatography, cation exchange liquid chromatography or reversed phase liquid chromatography to carry out gradient elution to the sample to be tested,
and (3) detecting and analyzing corresponding peak areas of all components of the copolymer, and comparing the peak areas with a reference substance to determine whether the content range of each component of the sample to be detected is qualified.
The elution gradient in the step (2) is
The chromatographic conditions of the anion exchange liquid chromatography adopted in the step (2) are as follows:
adopting a chromatographic column with carboxyl bonded polystyrene-divinylbenzene particles as a filler, wherein the particle size of the filler particles of the chromatographic column is 1.7-10 mu m;
taking trihydroxyaminomethane hydrochloride solution containing 10-50mM as a mobile phase A, and adjusting the pH value of the mobile phase A to 10-12 by using NaOH solution;
taking solution containing 10-50mM trihydroxy aminomethane hydrochloride and 0.5-1.5M sodium chloride as mobile phase B, and adjusting the pH value of the mobile phase B to 8-10 by hydrochloric acid solution;
the sample amount is 5-50uL, the sample concentration is 1-20mg/ml,
the flow rate is 0.5-1.5mL/min, and the elution time is 50-250 min;
the elution gradient was:
elution is carried out by adopting elution gradients of different forms,
the column temperature is 25-50 ℃;
the elution gradient is as follows:
the total elution time is equally divided into N (N is more than or equal to 2 and less than or equal to 20) steps, gradient elution is sequentially carried out, the proportion of the mobile phase A in the gradient of the first N-1 step is gradually reduced from 100 percent to 50 percent, the proportion of the mobile phase B is gradually increased from 0 to 50 percent, the proportion of the mobile phase A in the Nth step is 100 percent, and the proportion of the mobile phase B is 0;
preferably 5. ltoreq. N.ltoreq.15, more preferably 8. ltoreq. N.ltoreq.12, most preferably, N10.
The chromatographic conditions of the cation exchange liquid chromatography adopted in the step (2) are as follows:
the method comprises the following steps of (1) adopting a chromatographic column taking tertiary ammonium group bonded polystyrene-divinylbenzene particles as a filler, wherein the particle size of the filler particles of the chromatographic column is 1.7-10 mu m;
using a solution containing 10-50mM of 2- (N-morpholino) ethanesulfonic acid and 0.5-5mM of EDTA as a mobile phase A, and adjusting the pH value to 4-6 by using a NaOH solution;
using solution containing 10-50mM 2- (N-morpholino) ethanesulfonic acid, 0.5-5mM EDTA and 1-2.5M NaCl as mobile phase B, and adjusting pH value to 5-7 with NaOH solution;
the sample amount is 5-50uL, the sample concentration is 1-20mg/ml,
the flow rate is 0.5-1.5mL/min, and the elution time is 50-250 min;
the elution gradient was:
elution is carried out by adopting elution gradients of different forms,
the column temperature is 25-50 ℃;
the elution gradient is as follows:
the total elution time is equally divided into N (N is more than or equal to 2 and less than or equal to 20) steps, gradient elution is sequentially carried out, the proportion of the mobile phase A in the first N-1 step of gradient is gradually reduced from 100 percent to 0 percent, the proportion of the mobile phase B is gradually increased from 0 to 100 percent, the proportion of the mobile phase A in the Nth step is 90 percent, and the proportion of the mobile phase B in the Nth step is 10 percent;
preferably 5. ltoreq. N.ltoreq.15, more preferably 8. ltoreq. N.ltoreq.12, most preferably, N10.
The chromatographic conditions of the reversed phase liquid chromatography adopted in the step (2) are as follows:
a chromatographic column taking C18, C12, C8 and C4 bonded silica gel particles as fillers is adopted, and the particle size of the chromatographic column filler particles is 1.7-10 mu m;
acetonitrile is used as a mobile phase A;
taking a solution containing 30-80mM ammonium sulfate as a mobile phase B, and adjusting the pH value to 2-3 by using a phosphoric acid solution;
or 0.1% trifluoroacetic acid solution is used as mobile phase B;
the sample amount is 5-50uL, the sample concentration is 1-20mg/ml,
the flow rate is 0.5-1.5mL/min, and the elution time is 50-250 min;
the elution gradient was:
elution is carried out by adopting elution gradients of different forms,
the column temperature is 25-50 ℃;
the elution gradient is as follows:
the total elution time is equally divided into N (N is more than or equal to 2 and less than or equal to 20) steps, gradient elution is sequentially carried out, the proportion of the mobile phase A in the gradient of the first N-1 step is gradually increased from 5 percent to 40 percent, the proportion of the mobile phase B is gradually decreased from 95 percent to 60 percent, the proportion of the mobile phase A in the Nth step is 5 percent, and the proportion of the mobile phase B in the Nth step is 95 percent;
preferably 5. ltoreq. N.ltoreq.15, more preferably 8. ltoreq. N.ltoreq.12, most preferably, N10.
The detection analysis in the step (3) adopts an ultraviolet detector to detect the wavelength of 260-280 nm;
when a fluorescence detector is used, the excitation wavelength is 230nm and the emission wavelength is 300 nm.
Drawings
FIG. 1-1, example 1 chromatogram of test samples.
FIGS. 1-2, example 2 chromatogram of test samples.
FIGS. 1-3, example 3 chromatogram of test samples.
FIGS. 1-4, example 4 chromatogram of test samples.
FIGS. 1-5, example 5 chromatogram of test samples.
FIG. 2-1, example 7 chromatogram of test samples.
FIG. 2-2, example 8 chromatogram of test samples.
FIGS. 2-3, example 9 chromatogram of test samples.
FIGS. 2-4, example 10 chromatogram of test samples.
FIG. 3-1, example 12 chromatogram of test samples.
FIG. 3-2, example 13 chromatogram of test samples.
FIGS. 3-3, example 14, chromatography of test samples.
FIGS. 3-4, example 15 chromatogram of test samples.
FIGS. 3-5, example 16, chromatographic analysis of test samples.
Detailed Description
Example 1
A2475 fluorescence multi-wavelength detector of a Watt-off 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 150mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is polystyrene-divinylbenzene particles, and the particle size of the chromatographic column is 3 mu m. Mobile phase a was 20mM tris hydrochloride, adjusted to pH 11.2 with NaOH solution; mobile phase B was 20mM tris hydrochloride, containing 1M NaCl, adjusted to pH 9.8 with hydrochloric acid solution, and the ratio of mobile phases a and B was set according to table 1 below. Commercially available glatiramer acetate (hereinafter referred to as a reference substance) and a test sample glatiramer acetate (hereinafter referred to as a test sample) were separately added with a mobile phase to dissolve and prepare a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was set at 0.8mL/min, and the column temperature was 30 ℃. The proportions of the components are shown in Table 2, and the chromatogram analysis chart of a certain sample is shown in attached figure 1-1.
Table 1 shows the ratio of mobile phases A and B in example 1
Table 2 shows the comparison results of anion exchange between 3 control samples and 3 test samples
Example 2
A2475 fluorescence multi-wavelength detector of a Vortet 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 150mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is polystyrene-divinylbenzene particles, and the particle size of the chromatographic column is 5 microns. Mobile phase a was 10mM tris hydrochloride, adjusted to pH 10 with NaOH solution; mobile phase B was 10mM tris hydrochloride containing 1.5M NaCl, adjusted to pH 8 with hydrochloric acid solution, and the ratio of mobile phases a and B was set according to table 1. The control or the test sample is dissolved in the mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample introduction amount was 15. mu.L, the flow rate was set at 0.5mL/min, and the column temperature was 25 ℃. A chromatogram of a sample is shown in figures 1-2.
Example 3
A Watt 2695 high performance liquid chromatograph 2489 is used as an ultraviolet multi-wavelength detector, the wavelength is 275nm, the size of a chromatographic column is 150mm long, the diameter of the chromatographic column is 4.6mm, and the filler of the chromatographic column is polystyrene-divinylbenzene particles with the particle size of 10 mu m. Mobile phase a was 50mM tris hydrochloride, adjusted to pH 12 with NaOH solution; mobile phase B was 50mM tris hydrochloride containing 0.5M NaCl, adjusted to pH 10 with hydrochloric acid solution, and the ratio of mobile phases a and B was set according to table 1. The control or test sample is dissolved in mobile phase and made into a solution containing about 10mg per 1mL for testing. The sample volume was 50. mu.L, the flow rate was set at 1.5mL/min, and the column temperature was 50 ℃. A sample chromatogram is shown in figures 1-3
Example 4
A Watt 2695 high performance liquid chromatograph 2489 is used as an ultraviolet multi-wavelength detector, the wavelength is 275nm, the size of a chromatographic column is 150mm long, the diameter of the chromatographic column is 4.6mm, and the filler of the chromatographic column is polystyrene-divinylbenzene particles with the particle size of 3 mu m. Mobile phase a was 30mM tris hydrochloride, adjusted to pH 10 with NaOH solution; mobile phase B was 30mM tris hydrochloride containing 1M NaCl, adjusted to pH 8 with hydrochloric acid solution, and the ratio of mobile phases a and B was set according to table 3 below. The control or the test sample is dissolved in the mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was set at 0.8mL/min, and the column temperature was 40 ℃. A chromatogram of a sample is shown in figures 1-4.
Table 3 example 4 ratio of mobile phases a and B
Time (min)
Mobile phase A (%)
Mobile phase B (%)
0
100
0
15
95
5
30
92.5
7.5
45
90
10
60
87.5
12.5
75
85
15
90
80
20
105
70
30
120
50
50
135
100
0
150
100
0
Example 5
A2475 fluorescence multi-wavelength detector of a Watt-off 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 150mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is polystyrene-divinylbenzene particles, and the particle size of the chromatographic column is 3 mu m. Mobile phase a was 20mM tris hydrochloride, adjusted to pH 11.2 with NaOH solution; mobile phase B was 20mM tris hydrochloride, containing 1M NaCl, adjusted to pH 9.8 with hydrochloric acid solution, and the ratio of mobile phases a and B was set according to table 4 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was 1mL/min, and the column temperature was 30 ℃. A sample chromatogram is shown in FIGS. 1-5, and it can be seen that under this elution condition, the components of the sample cannot be separated efficiently.
TABLE 4 example 5 ratio of mobile phases A to B
Time (min)
Mobile phase A (%)
Mobile phase B (%)
0
100
0
10
100
0
30
0
100
40
0
100
41
100
0
55
100
0
Example 6
A Watt 2695 high performance liquid chromatograph 2489 is used as an ultraviolet multi-wavelength detector, the wavelength is 275nm, the size of a chromatographic column is 150mm long, the diameter of the chromatographic column is 4.6mm, and the filler of the chromatographic column is polystyrene-divinylbenzene particles with the particle size of 3 mu m. Mobile phase a was 20mM tris hydrochloride, adjusted to pH 11.2 with NaOH solution; mobile phase B was 20mM tris hydrochloride, containing 1M NaCl, adjusted to pH 9.8 with hydrochloric acid solution, and the ratio of mobile phases a and B was set according to table 4 below. The reference substance and the trial sample are respectively taken, and the mobile phase is added for dissolution to prepare a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was set at 0.8mL/min, and the column temperature was 30 ℃. The proportions of the components are shown in Table 6.
Table 5 shows the ratio of mobile phases A and B in example 6
Table 6 shows the comparison of anion exchange compositions of 3 control samples and 3 test samples
Cation exchange liquid chromatography (examples 7 to 11)
Example 7
A2475 fluorescence multi-wavelength detector of a Watt-off 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 250mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is tertiary ammonium group bonded polystyrene-divinylbenzene particles, and the particle size is 5 mu m. Mobile phase a was 20mM 2- (N-morpholino) ethanesulfonic acid, containing 1mM EDTA, adjusted to pH 5.2 with NaOH solution; mobile phase B was 20mM 2- (N-morpholino) ethanesulfonic acid, containing 2mM EDTA, containing 2M NaCl, adjusted to pH 5.8 with NaOH solution, and the ratio of mobile phases a and B was set according to table 7 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was 1mL/min, and the column temperature was 30 ℃. A chromatogram of a sample is shown in figure 2-1.
Table 7 shows the ratio of mobile phases A and B in example 7
Time (min)
Mobile phase A (%)
Mobile phase B (%)
0
90
10
15
90
10
15.1
80
20
30
80
20
30.1
70
30
45
70
30
45.1
60
40
60
60
40
60.1
50
50
75
50
50
75.1
40
60
90
40
60
90.1
30
70
105
30
70
105.1
20
80
120
20
80
120.1
0
100
135
0
100
135.1
90
10
150
90
10
Table 8 shows the comparison of the cation exchange compositions of the 3 control samples and the 3 test samples
Example 8
A Watt 2695 high performance liquid chromatograph 2489 is used as an ultraviolet multi-wavelength detector, the wavelength is 275nm, the size of a chromatographic column is 250mm long and the diameter is 4.6mm, and the filler of the chromatographic column is tertiary ammonium-based bonded polystyrene-divinylbenzene particles with the particle size of 10 mu m. Mobile phase a was 10mM 2- (N-morpholino) ethanesulfonic acid, containing 0.5mM EDTA, adjusted to pH 4 with NaOH solution; mobile phase B was 10mM 2- (N-morpholino) ethanesulfonic acid, containing 5mM EDTA, containing 2M NaCl, adjusted to pH 7 with NaOH solution, and the ratio of mobile phases a and B was set according to table 7. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample introduction amount was 15. mu.L, the flow rate was set at 1mL/min, and the column temperature was 25 ℃. A sample chromatogram is shown in figure 2-2
Example 9
A2475 fluorescence multi-wavelength detector of a Watt-off 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 250mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is tertiary ammonium group bonded polystyrene-divinylbenzene particles, and the particle size is 5 mu m. Mobile phase a was 50mM 2- (N-morpholino) ethanesulfonic acid, containing 1mM EDTA, adjusted to pH 6 with NaOH solution; mobile phase B was 50mM 2- (N-morpholino) ethanesulfonic acid, containing 2mM EDTA, 2M NaCl, adjusted to pH 5 with NaOH solution, and the ratio of mobile phases a and B was set according to table 7 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 10mg per 1mL for testing. The sample volume was 50. mu.L, the flow rate was set at 1mL/min, and the column temperature was 30 ℃. A chromatogram of a sample is shown in figures 2-3.
Example 10
A2475 fluorescence multi-wavelength detector of a Watt-off 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 250mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is tertiary ammonium group bonded polystyrene-divinylbenzene particles, and the particle size is 5 mu m. Mobile phase a was 20mM 2- (N-morpholino) ethanesulfonic acid, containing 1mM EDTA, adjusted to pH 5.2 with NaOH solution; mobile phase B was 20mM 2- (N-morpholino) ethanesulfonic acid, containing 2mM EDTA, 2M NaCl, adjusted to pH 5.8 with NaOH solution, and the ratio of mobile phases a and B was set according to table 9 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was 1mL/min, and the column temperature was 30 ℃. A sample chromatogram is shown in FIGS. 2-4, and it can be seen that under this elution condition, the components of the sample cannot be separated efficiently.
TABLE 9 example 10 ratio of mobile phases A to B
Example 11
A Watt 2695 high performance liquid chromatograph 2489 is used as an ultraviolet multi-wavelength detector, the wavelength is 275nm, the size of a chromatographic column is 250mm long and the diameter is 4.6mm, and the filler of the chromatographic column is tertiary ammonium-based bonded polystyrene-divinylbenzene particles with the particle size of 5 mu m. Mobile phase a was 30mM 2- (N-morpholino) ethanesulfonic acid, containing 3mM EDTA, adjusted to pH 5 with NaOH solution; mobile phase B was 10mM 2- (N-morpholino) ethanesulfonic acid, 2mM EDTA, 2M NaCl, adjusted to pH 6 with NaOH solution, and the ratio of mobile phases a and B was set according to table 10 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was 1mL/min, and the column temperature was 35 ℃. The proportions of the components are shown in Table 11.
Table 10 shows the ratio of mobile phases A and B in example 11
Table 11 shows the comparison of the cation exchange compositions of the 3 control samples and the 3 test samples
Reverse phase liquid chromatography case (examples 12 to 17)
Example 12
An Agilent 1260 high performance liquid chromatograph ultraviolet multi-wavelength detector is used, the detection wavelength is set to 275nm, the size of a chromatographic column is 250mm long, the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is silica gel matrix bonded C18 ligand particles, and the particle size is 3 microns. Mobile phase a is acetonitrile; mobile phase B was a 50mM ammonium sulfate solution adjusted to pH 2.5 with phosphoric acid solution and the ratio of mobile phases a and B was set as in table 12 below. The control and the test sample were dissolved in mobile phase and made into solutions containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was 1mL/min, and the column temperature was 30 ℃. The component ratio analysis of the reference sample and the test sample is shown in Table 13, and the chromatogram analysis of a certain sample is shown in figure 3-1.
Table 12 is the ratio of mobile phases a and B of example 12:
table 13 shows the comparison of the reversed phase analysis of each component of the 3 control samples and the 3 test samples
Example 13
An Agilent 1260 high performance liquid chromatograph ultraviolet multi-wavelength detector is used, the detection wavelength is set to 275nm, the size of a chromatographic column is 250mm long, the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is silica gel matrix bonded C8 ligand particles, and the particle size is 10 microns. Mobile phase a is acetonitrile; mobile phase B was a 0.1% trifluoroacetic acid solution and the ratio of mobile phases a and B was set as in table 12. The control and the test sample were dissolved in mobile phase and made into solutions containing about 10mg per 1mL for testing. The sample volume was 50. mu.L, the flow rate was 1mL/min, and the column temperature was 50 ℃. A chromatogram of a sample is shown in figure 3-2.
Example 14
An Agilent 1260 high performance liquid chromatograph ultraviolet multi-wavelength detector is used, the detection wavelength is set to 275nm, the size of a chromatographic column is 250mm long, the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is silica gel matrix bonded C4 ligand particles, and the particle size is 3 microns. Mobile phase a is acetonitrile; mobile phase B was a 30mM ammonium sulfate solution adjusted to pH 2 with phosphoric acid solution and the ratio of mobile phases a and B was set as in table 12. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample introduction amount was 15. mu.L, the flow rate was set at 0.5mL/min, and the column temperature was 25 ℃. A chromatogram of a sample is shown in figure 3-3.
Example 15
An Agilent 1260 high performance liquid chromatograph ultraviolet multi-wavelength detector is used, the detection wavelength is set to 275nm, the size of a chromatographic column is 250mm long, the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is silica gel matrix bonded C4 ligand particles, and the particle size is 3 microns. Mobile phase a is acetonitrile; mobile phase B was an 80mM ammonium sulfate solution adjusted to pH 3 with phosphoric acid solution and the ratio of mobile phases a and B was set as in table 12. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 25. mu.L, the flow rate was set at 0.5mL/min, and the column temperature was 50 ℃. A chromatogram of a sample is shown in figures 3-4.
Best mode for carrying out the invention
A2475 fluorescence multi-wavelength detector of a Vorter 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 250mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is silica gel matrix bonded C18 ligand particles, and the particle size is 3 microns. Mobile phase a is acetonitrile; mobile phase B was a 0.1% trifluoroacetic acid solution and the ratio of mobile phases a and B was set as in table 14 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 50. mu.L, the flow rate was set at 1mL/min, and the column temperature was 30 ℃. The chromatogram analysis of a sample is shown in figures 3-5, which shows that the conventional method can not separate each component in the trial sample.
TABLE 14 example 16 ratio of mobile phases A to B
Example 17
A2475 fluorescence multi-wavelength detector of a Vorter 2695 high performance liquid chromatograph is used, the excitation wavelength (Ex) is 230nm, the emission wavelength (Em) is 300nm, the size of a chromatographic column is 250mm long and the diameter of the chromatographic column is 4.6mm, the filler of the chromatographic column is silica gel matrix bonded C18 ligand particles, and the particle size is 3 microns. Mobile phase a is acetonitrile; mobile phase B was a 0.1% trifluoroacetic acid solution and the ratio of mobile phases a and B was set as in table 15 below. The control or test sample is dissolved in mobile phase and made into a solution containing about 20mg per 1mL for testing. The sample volume was 50. mu.L, the flow rate was set at 1.5mL/min, and the column temperature was 30 ℃. The proportions of the components are shown in Table 16.
Table 15 shows the ratio of mobile phases A to B in example 17
Time (min)
Mobile phase A (%)
Mobile phase B (%)
0
100
0
15
100
0
15.1
90
10
30
90
10
30.1
80
20
45
80
20
45.1
70
30
60
70
30
60.1
60
40
75
60
40
75.1
55
45
85
55
45
85.1
50
50
100
50
50
100.1
100
0
120
100
0
Table 16 shows the comparison of the reversed phase analysis of each component of the 3 control samples and the 3 test samples
From the above examples, it can be seen that the method for analyzing the content of each component in the glatiramer acetate sample is effective and feasible, and can be used for comparative analysis and quality consistency research of the glatiramer acetate.
Finally, it should be noted that the above examples are only used to help those skilled in the art understand the essence of the present invention, and should not be construed as limiting the scope of the present invention.
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