阅读说明：本技术 冬凌草甲素在制备治疗高尿酸血症和抗痛风药物中的应用 (Application of oridonin in preparing medicine for treating hyperuricemia and anti-gout ) 是由 徐霞 王馨迎 崔伟琪 可钰 于 2020-12-21 设计创作，主要内容包括：本发明属药物化学领域,涉及冬凌草甲素在制备治疗高尿酸血症和抗痛风药物中的应用。本发明提供了冬凌草甲素作为唯一有效成分或主要有效成分用于制备治疗高尿酸血症和抗痛风药物的新用途。冬凌草甲素在小鼠中的应用量按10 mg/kg体重量灌胃给药,给药后可降低血清尿酸水平,抑制肝脏组织中的黄嘌呤氧化酶活性,产生治疗高尿酸血症和抗痛风效果,且具有用量小,对肝、肾无明显毒性等优点。本发明为冬凌草甲素开拓了新的用途,并为改善目前抗痛风病药物副作用大的现象提供了新的方向。(The invention belongs to the field of pharmaceutical chemistry, and relates to application of oridonin in preparation of a medicament for treating hyperuricemia and gout. The invention provides a new application of oridonin as a unique effective component or a main effective component in preparing medicaments for treating hyperuricemia and gout. The application amount of oridonin in mice is administrated by gavage according to the weight amount of 10mg/kg, after administration, the oridonin can reduce the serum uric acid level, inhibit the xanthine oxidase activity in liver tissues, and produce the effects of treating hyperuricemia and resisting gout, and has the advantages of small dosage, no obvious toxicity to liver and kidney, and the like. The invention develops new application of oridonin and provides a new direction for improving the phenomenon of large side effect of the existing anti-gout drugs.)

1. The application of the oridonin in preparing the medicine for treating hyperuricemia and anti-gout is characterized in that the oridonin is used as an effective component to prepare the medicine for treating hyperuricemia or anti-gout.

2. The use of oridonin according to claim 1 in the preparation of a medicament for the treatment of hyperuricemia and gout, wherein the composition of the medicament for the treatment of hyperuricemia or gout comprises pharmaceutically acceptable auxiliary components.

3. The use of oridonin according to claim 1 or 2 in the preparation of a medicament for the treatment of hyperuricemia or gout, wherein the medicament for the treatment of hyperuricemia or gout is in a dosage form selected from the group consisting of powder, granule, tablet, capsule, pill, solution, suspension, and injection.

Technical Field

The invention relates to a pharmaceutical technology, belongs to the field of pharmaceutical chemistry, and particularly relates to an application of oridonin in preparation of a medicine for treating hyperuricemia and gout.

Background

Uric acid (uric acid), 2,6, 8-trioxypurine, is the end product of purine metabolism in humans. Uric acid in blood is produced by oxidation of xanthine and hypoxanthine catalyzed by xanthine oxidase from butter cream (XOD), and plays an important physiological role in protecting nerve cells, maintaining blood pressure, and the like. Under normal purine diet, the level of uric acid in fasting blood twice on non-same day is higher than 420 mu mol/L in male and higher than 360 mu mol/L in female, namely Hyperuricemia (HUA), at the moment, uric acid can be separated out and crystallized and deposited in tissues to cause histological changes, and gout, gouty arthritis, gouty nephropathy and the like are caused. Hyperuricemia often accompanies cardiovascular diseases, and is closely related to hypertension, hyperlipidemia, insulin resistance, atherosclerosis, obesity, and the like. In China, the prevalence rate of HUA is as high as 13.3%, and in recent years, with the improvement of living standard of people and the change of dietary structure, the prevalence rate of hyperuricemia is on the trend of rising year by year.

Currently, the clinical common uric acid lowering drugs are mainly divided into two categories, namely inhibiting the production of uric acid and promoting the excretion of uric acid. The medicament for inhibiting the generation of uric acid is mainly a Xanthine Oxidase Inhibitor (XOI) which mainly contains allopurinol, but allopurinol has a purine structure, possibly influences the activities of other enzymes in purine metabolism and pyrimidine metabolism, can cause adverse reactions such as liver and kidney injury, allergy, bone marrow transplantation and the like, and inhibits the clinical use of the allopurinol to a certain extent. Febuxostat is a selective non-purine xanthine oxidase inhibitor, can specifically inhibit reduced xanthine oxidase and oxidized xanthine oxidase, has a better effect of reducing uric acid and higher safety compared with allopurinol, but has also been reported to have adverse reactions such as abnormal liver function, gastrointestinal reaction, arthralgia and the like. Drugs for promoting uric acid excretion include probenecid, benzbromarone, etc., and act on urate transporters of renal proximal convoluted tubule to inhibit uric acid absorption. But researches find that probenecid and benzbromarone have side effects of gastrointestinal reaction, renal colic, gout acute attack excitation and the like. Therefore, the existing uric acid reducing medicines on the market are limited, and have obvious toxic and side effects and poor patient compliance. Therefore, the components with small toxic and side effects in the traditional Chinese medicine treasury are selected to develop the novel high-efficiency low-toxicity uric acid reducing medicine with the targeting effect, and the medicine has important effects and significance.

Rabdosia rubescens belongs to labiate plants, is a genuine medicinal material of Henan, is produced in Henan Taihang mountain range, is a plant medicine which is excavated from folks in Henan province in the early eighties of the last century, and has the effects of clearing heat, reducing internal heat, dredging qi and blood and relieving pain. According to reports, the rabdosia rubescens boiled tea is used for treating the sore throat in local places of Jiyuan in Henan province. Research shows that rabdosia rubescens has significant anti-inflammatory activity, and the anti-inflammatory activity mainly comes from diterpenoid compounds, especially Oridonin. Oridonin is the first tetracyclic diterpenoid compound extracted from Rabdosia Rubescens (Hemsl.) Hara, and has good antiinflammatory activity and antitumor activity. Oridonin has been reported to inhibit more than twenty common tumor cells, including: esophageal cancer, pancreatic cancer, liver cancer, etc. At present, the application of oridonin as a drug effect component for preventing and/or treating hyperuricemia and gout is not reported.

Disclosure of Invention

Based on the accidental discovery of the inventor, the invention provides a new application of oridonin serving as a unique effective component or a main effective component in preparing a medicament for treating hyperuricemia and anti-gout.

In order to achieve the above purpose, the invention provides the following technical scheme:

application of rubescensine A in preparing medicine for treating hyperuricemia and gout is provided. Oridonin molecular formula C₂₀H₂₈O₆Molecular weight 364.44, the structure is shown in formula 1.

Further, the application of the oridonin in preparing the medicaments for treating hyperuricemia and gout is that the application amount of the oridonin in mice is administrated by gastric gavage according to the weight of 10 mg/kg.

Mainly takes a male Kunming mouse as an experimental object, induces a hyperuricemia model through yeast powder and Potassium Oxonate, intervenes by gastric lavage of a traditional Chinese medicine extract oridonin, and verifies the application of the oridonin in treating hyperuricemia and resisting gout. The results show that: compared with a model control group, the serum uric acid level of mice in the oridonin administration group is reduced, and the xanthine oxidase activity in liver tissues is reduced; compared with the normal saline group, the levels of liver function enzyme and kidney function enzyme of mice in the oridonin administration group have no obvious change. Therefore, the oridonin has the functions of treating hyperuricemia and resisting gout, has no obvious toxicity to liver and kidney, and can be used for preparing medicines for treating hyperuricemia and resisting gout.

The medicament for treating hyperuricemia and anti-gout comprises the oridonin, can be a single-component medicament only containing the oridonin, and can also be a pharmaceutical composition containing the oridonin. The pharmaceutical composition containing the oridonin comprises the oridonin and a pharmaceutical component which has positive effects on treating hyperuricemia and resisting gout after being simultaneously applied with the oridonin and/or a pharmaceutically acceptable component and/or a pharmaceutically acceptable auxiliary component for improving the stability of the oridonin.

The dosage form of the drug for treating hyperuricemia and anti-gout provided by the invention can be powder, granules, tablets, capsules, pills, solution, suspension or injection and the like.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, oridonin is used for preparing the medicine for treating hyperuricemia and gout, and the medicine can reduce the serum uric acid level of mice, inhibit the activity of xanthine oxidase in liver tissues, has remarkable effects of treating hyperuricemia and gout, has the advantages of small dosage, no obvious toxicity to liver and kidney and the like. Develops a new application of the oridonin and provides a new direction for improving the phenomenon of large side effect of the existing anti-gout drugs.

Drawings

FIG. 1 is a schematic diagram showing the effect of oridonin on the reduction of mouse serum uric acid level.

FIG. 2 is a schematic diagram showing the effect of oridonin on the inhibition of mouse liver xanthine oxidase.

FIG. 3 is a schematic diagram showing the effect of oridonin on the level changes of mouse serum liver function enzymes (AST, ALT) and kidney function enzymes (CRE-E, BUN). Wherein, the graph A is a statistical graph of the level of aspartate Aminotransferase (AST) in the serum of the mice; panel B is a statistical plot of alanine Aminotransferase (ALT) levels in mouse serum; panel C is a statistical plot of creatinine (CRE-E) levels in mouse serum; panel D is a statistical plot of urea (BUN) levels in mouse serum.

FIG. 4 is a schematic view of molecular docking simulation of oridonin and xanthine oxidase protein (2 CKJ).

Detailed Description

The present invention will be described in further detail with reference to specific embodiments for better illustrating the objects, technical solutions and advantages of the present invention, but the present invention is not limited to the following examples.

In the following embodiments, the biochemical reagents not specifically described are all conventional reagents in the art, and can be prepared according to conventional methods in the art or obtained commercially, and the specification is laboratory pure grade, and the percentages are all mass percentages unless specifically described.

Test method

The source of the drug is as follows: the medicament rubescensine A is purchased from Sienna Hao Xuan Biotech limited company, and the purity is more than 98 percent; the febuxostat drug is purchased from Shanghai Alatin Biotechnology GmbH, and the purity is more than or equal to 98 percent; potassium oxonate was purchased from Sigma Aldrich (Shanghai) trade, Inc. with a purity of > 98%; yeast powder was purchased from Obo Star Biotechnology, Inc., Beijing.

Preparing the medicine: oridonin is prepared into 1mg/mL suspension by using 0.5% CMC-Na; preparing yeast powder into 3g/mL suspension by using normal saline; potassium oxonate is prepared into a suspension of 25mg/mL by using 0.5 percent CMC-Na; febuxostat (positive protective drug) is prepared into suspension with the concentration of 4mg/mL by using 0.5% CMC-Na.

Animal feeding: 40 healthy male Kunming mice with the weight of 18-20g are provided by animal experiment centers in Henan province, and license numbers are as follows: SCXK (Yu) 2017-. The breeding environment is SPF grade, independent air intake and return purification Cages (IVC) are arranged, the temperature is constant (22 +/-2 ℃), the humidity is constant (55 +/-15%), light and shade are alternated every 12 hours, one cage is arranged for every 5 mice, autoclaved water is drunk, and the mice can eat freely.

The administration mode comprises the following steps: oridonin (10mg/kg), febuxostat (40mg/kg), yeast powder (30g/kg) and normal saline adopt a gastric perfusion administration mode, and oteracil potassium (250mg/kg) adopts an intraperitoneal injection administration mode.

Example 1

The effect of oridonin on hyperuricemia induced by yeast powder and Potassium Oxonate.

40 healthy male Kunming mice with the weight of 18-20g are provided by the animal experiment center in Henan province. The mice are adaptively raised in SPF level animal rooms of the institute of medicine of Zhengzhou university, Henan province for one week, and are divided into 4 groups by a random number table method, wherein each group comprises 10 mice, and the specific steps are as follows:

1. a normal control group;

2. a model control group;

3. oridonin group;

4. a positive control drug group.

Dosing was started after the end of the acclimatization period, and gavage twice daily in the morning. Firstly, performing intragastric administration on a model control group and an oridonin group, wherein a positive control group adopts yeast powder (30g/kg), and a normal control group is administered with equivalent physiological saline for intragastric administration; after 1h, the oridonin group was subjected to intragastric administration (10mg/kg), the positive control group was subjected to intragastric administration (40mg/kg) of febuxostat, and the model control group and the normal control group were subjected to intragastric administration of 0.5% CMC-Na in equal amounts. The body weight of the mice was weighed and recorded daily for seven consecutive days.

1h after the two intragastric gavage operations are finished in the morning on the seventh day, the model control group, the oridonin group and the positive control group are injected with oteracil potassium (250mg/kg) in the abdominal cavity, and the normal control group is injected with 0.5 percent of CMC-Na in the same amount in the abdominal cavity.

After intraperitoneal injection for 1h, the eyeball is removed and blood is taken. Standing the obtained blood at room temperature for half an hour, centrifuging at 3500r for 5min, and retaining supernatant to obtain serum. Serum uric acid levels, liver function enzymes (ALT, AST) and kidney function enzymes (BUN, CRE-E) levels were determined using a fully automated biochemical analyzer (BX 4000). The mouse liver and kidney were harvested and washed with PBS solution, blotted dry with filter paper, and stored in a freezer at-80 ℃.

50mg of liver tissue was weighed, homogenized with physiological saline (0.9%) pre-cooled in a refrigerator, centrifuged at 12000rpm at 4 ℃ for 10min, and the supernatant was retained to determine the enzymatic activity of xanthine oxidase with reference to the kit instructions (colorimetric method, cat # A002-1-1).

The protein structure of xanthine oxidase (2ckj) was obtained by obtaining the RCSB protein database, searching all binding sites of 2ckj using the corsite program to predict the likely binding site of oridonin, and calculating the Z value and the pharmaceutical utility of the binding site. Inputting oridonin and xanthine oxidase protein into AutoDock-4.2 software, and performing molecular simulation docking. And finally obtaining a three-dimensional structure image of the combination of the oridonin and the xanthine oxidase by PyMol-1.6.x software.

The statistical analysis method comprises the following steps:

the results are expressed as means ± SE and the comparison of the mean of the two samples is performed by t-test.

As shown in Table 1 and FIG. 1, the serum uric acid level of mice in the model control group was significantly increased (P < 0.05) compared with the normal control group, indicating that the molding was successful. Compared with the model control group, the uric acid level of the serum of the mice in the oridonin group is reduced from 26.62 +/-2.63 to 12.39 +/-2.65 (P is less than 0.05), which indicates that the oridonin has good effect of reducing uric acid.

As shown in Table 2 and FIG. 2, the XOD activity in the mouse liver in the model control group was significantly increased (P < 0.05) compared to the normal control group, and the XOD activity in the mouse liver in the oridonin group was decreased from 14.37 + -2.11 to 10.21 + -2.88 (P < 0.05) compared to the model control group, indicating that oridonin exerts a good uric acid lowering effect by inhibiting the XOD activity.

The results are shown in A in figure 3, compared with the normal control group, the ALT level of the model control group is obviously increased (P < 0.01), which indicates that the hyperuricemia mouse is possibly accompanied with the occurrence of liver injury, and the ALT level increase caused by the hyperuricemia model can be obviously reduced by the oridonin (P < 0.01); as shown in B-D in FIG. 3, there was no significant difference in the levels of AST, BUN and CRE-E in the sera of mice in the normal control group and oridonin group. The oridonin has no influence on liver and kidney functions and has the potential of liver protection.

The results of molecular simulation docking of oridonin and xanthine oxidase protein using AutoDock-4.2 software showed that the lowest scoring binding site was cavity5 (score-5.066). As a result, as shown in FIG. 4, the ketone carbonyl group in the oridonin structure can form hydrogen bonds with SER-1081 and GLN-1041, and the hydroxyl group can form hydrogen bonds with ARG-913, therefore, oridonin may be bound with cavity5 of protein 2ckj through hydrogen bonds, thereby inhibiting the enzymatic activity of XOD.

TABLE 1 Effect of Oridonin on Potassium Oxonate and Yeast powder induced uric acid levels in serum of mice with hyperuricemia

Group of	Dosage form	Uric acid (mu mol/L)
			Normal control group	0.9％NaCl	12.71.±2.71
Model control group	30g/kg+250mg/kg	26.62±2.63#
			Oridonin group	10mg/kg	12.39±2.65*
Positive control group	40mg/kg	14.98±1.23*

Note: #: p <0.05 compared to normal control; # #: p <0.01 compared to normal control group

*: p <0.05, compared to model control group: p <0.01 compared to model control.

TABLE 2 Effect of oridonin on Potassium Oxonate and Yeast powder combination induced hyperuricemia mouse liver XOD Activity

Group of	Dosage (mg/kg)	XOD(U/mg)
			Normal control group	0.9％NaCl	11.14±2.07
Model control group	30g/kg+250mg/kg	14.37±2.11#
			Oridonin group	10mg/kg	10.21±2.88*
Positive control group	40mg/kg	10.50±2.28*

Note: #: p <0.05 compared to normal control; # #: p <0.01 compared to normal control group

*: p <0.05, compared to model control group: p <0.01 compared to model control.