阅读说明：本技术 一种用于肝病快速筛查的便携式检测设备及检测方法 (Portable detection equipment and detection method for rapid screening of liver diseases ) 是由 王海容 韩宝庆 李长青 杨婉莹 陈家洛 吴昌龙 田汨龙 段滨 于 2020-06-03 设计创作，主要内容包括：本发明公开了一种用于肝病快速筛查的便携式检测设备及其检测方法,包括样品气体采样袋、惰性气体载气瓶、微型电磁阀集成模块、富集器、色谱柱、传感单元和单片机单元,微型电磁阀集成模块将流经微通道的样品气体和惰性气体分别流经富集器和色谱柱,进行通道切换后进入富集器或色谱柱；传感单元将进入的样品气体感应并进行电信号提取；通过单片机单元控制各模块对信号进行传递、交互,依据不同程度肝病特征气体的指纹特征,利用单片机单元中的模式识别算法进行肝病的快速筛查诊断。本发明具有智能化、检测成本低、简单安全、检测时间短、易携带等特点,可以用于家庭、社区、诊所等更广泛的场合进行初步的疾病筛查。(The invention discloses portable detection equipment for rapidly screening liver diseases and a detection method thereof, wherein the portable detection equipment comprises a sample gas sampling bag, an inert gas carrying cylinder, a micro electromagnetic valve integration module, an enricher, a chromatographic column, a sensing unit and a single chip microcomputer unit, wherein the micro electromagnetic valve integration module respectively flows sample gas and inert gas which flow through a micro channel through the enricher and the chromatographic column, and the sample gas and the inert gas enter the enricher or the chromatographic column after channel switching is carried out; the sensing unit senses the entering sample gas and extracts an electric signal; the single chip microcomputer unit controls each module to transmit and interact signals, and according to the fingerprint characteristics of the liver disease characteristic gases with different degrees, the mode recognition algorithm in the single chip microcomputer unit is utilized to carry out rapid screening diagnosis on the liver diseases. The invention has the characteristics of intellectualization, low detection cost, simplicity, safety, short detection time, easy carrying and the like, and can be used for primary disease screening in wider occasions such as families, communities, clinics and the like.)

1. A portable testing device for rapid screening of liver diseases, comprising:

the sampling bag is used for containing sample gas and is communicated with the micro electromagnetic valve integrated module;

the gas carrying bottle is used for carrying inert gas and is communicated with the micro electromagnetic valve integrated module;

the micro electromagnetic valve integration module is used for enabling the sample gas and the inert gas flowing through the micro channel to respectively flow through the enricher and the chromatographic column, and enabling the sample gas and the inert gas to enter the enricher or the chromatographic column after channel switching is carried out;

the enricher is used for realizing the adsorption and heating desorption of the sample gas;

the chromatographic column is used for separating the gas with complex characteristics in the sample gas on a time axis;

the sensing unit is used for sensing the sample gas entering the unit, extracting response electric signals and transmitting the acquired response information of the liver disease characteristic gases with different degrees to the single chip microcomputer unit;

the single chip microcomputer unit is used for controlling the opening and closing of the flow control modules of the sampling bag and the gas carrying bottle, the miniature electromagnetic valve and the vacuum pump; realizing the temperature closed-loop control of the heating/temperature measuring modules of the enriching device and the chromatographic column; power supply to the sensing unit and acquisition and processing of sensing signals;

the sampling bag and the gas carrying bottle are respectively communicated with the miniature electromagnetic valve integrated module, the miniature electromagnetic valve integrated module is respectively communicated with the enricher, the chromatographic column and the vacuum pump, the chromatographic column is communicated with the sensing unit, and the sensing unit, the vacuum pump, the chromatographic column, the enricher, the flow control module and the miniature electromagnetic valve integrated module are respectively connected with the single chip microcomputer unit.

2. The portable testing device for rapid screening of liver diseases according to claim 1, wherein the micro-solenoid valve integrated module has a channel mounting block and a plurality of micro-solenoid valves, the channel mounting block has micro-channels made of polymer material, and the plurality of micro-solenoid valves are respectively fixed on the channel mounting block.

3. The portable testing device for rapid screening of liver diseases according to claim 1, wherein the sampling bag and the gas carrying bottle are respectively connected to the micro-solenoid valve integrated module through a flow control module.

4. The portable detection device for rapid screening of liver diseases according to claim 1, wherein the concentrator contains micro-nano adsorption material and is provided with a heating/temperature measuring module.

5. The portable testing device for rapid screening of liver diseases according to claim 1, wherein the inner wall of the chromatographic column is coated with nano stationary phase material and provided with a heating/temperature measuring module.

6. The portable testing device for rapid screening of liver diseases according to claim 1, wherein the sensing unit comprises a number of metal oxide sensors and a humidity sensor.

7. The portable testing device for rapid screening of liver diseases according to claim 1, wherein the sensing unit is connected to the single chip microcomputer unit via a signal processing circuit; the single chip microcomputer unit is connected with a display and data processing module.

8. A portable test method for rapid screening of liver diseases based on the apparatus of any one of claims 1 to 7, comprising:

a gas sampling stage: the vacuum pump is started, sample gas in the sampling bag enters the enricher through the first flow control module and the micro electromagnetic valve integrated module to realize sample gas adsorption, and residual gas flows through the micro electromagnetic valve integrated module and is discharged from the outlet of the vacuum pump;

desorption and sample introduction stage: the enricher heats and desorbs the sample gas, the carrier gas flows through the microchannel of the miniature electromagnetic valve integrated module through the second flow control module, enters the enricher and pushes the desorbed sample gas to flow out of the enricher, and flows through the microchannel of the miniature electromagnetic valve integrated module again and then flows to the chromatographic column, the sample gas and the chromatographic column are fully acted, and the residual gas is discharged along the chromatographic column and the sensing unit in sequence;

a separation and cleaning stage: after the sample gas fully acts with the chromatographic column stationary phase, carrier gas with constant flow flows through the microchannel of the micro electromagnetic valve integrated module and enters the chromatographic column, all components of the separated sample gas sequentially flow out of the chromatographic column to the sensing unit, and the inductive electric signals of all sensors are transmitted to the single chip microcomputer unit; the first carrier gas is directly communicated until no sample gas is separated out from the chromatographic column;

an enrichment cleaning stage: and the carrier gas with constant flow directly enters the enricher through the micro electromagnetic valve integrated module microchannel, cleans the residual gas in the enricher, then flows through the micro electromagnetic valve integrated module microchannel, and is discharged from the outlet of the vacuum pump.

Technical Field

The invention relates to the field of liver disease detection, in particular to portable detection equipment for primary screening of liver diseases and a detection method thereof.

Background

In recent years, liver diseases are rapidly developed in the world, and developed and emerging countries are affected. By the end of 12 months in 2015, about 1.88, 4.31 and 1.01 billion major chronic liver disease cases exist in europe, china and the united states, respectively. The number of patients with hepatitis B, hepatitis C, alcoholic fatty liver disease and non-alcoholic fatty liver disease in the chronic liver disease patients in China is respectively about: 9314 ten thousand, 2431 ten thousand, 24635 ten thousand, 6827 ten thousand. According to a latest report from international cancer research center in 2018, the incidence of liver cancer in China is the ninth, and the number of liver cancer patients in China is estimated to be the largest globally according to population size. It is estimated that up to 700 million people in China have cirrhosis, that hepatitis B and C affect 9000 million people and 1000 million people, respectively, that non-alcoholic fatty liver disease affects about 1.73 to 3.38 million people, and that alcoholic liver disease affects at least 6200 million people. According to the prediction of professional research institutions, along with the more and more abundant physical lives of people, the liver diseases still further expand in the aspect of population prevalence in the future. Therefore, China and even the world face huge challenges in the aspects of early detection, diagnosis, treatment and the like of liver diseases.

The liver disease has the characteristic of dynamic evolution, about 20-30% of normal people usually have non-alcoholic fatty liver disease, about 7-30% of the normal people can develop into non-alcoholic fatty hepatitis, and about 10-20% of the normal people can develop into liver fibrosis till liver cirrhosis and liver cancer. If the accurate typing detection of the liver diseases can be carried out in the early stage, the early intervention can be carried out on high-risk patients in time, and the current situation of shortage of medical resources for liver disease detection can be relieved. Liver biopsy is currently the gold standard for clinical grading of liver fibrosis. This invasive procedure has a complication rate of about 5.9% and an alarming bleeding risk. Therefore, biopsy, a invasive, costly, complex and specialized detection method, is not suitable for screening a population for liver disease in a wide range. Therefore, it is necessary to find a non-invasive, simple, low-cost and safe detection means.

Compared with traditional biopsy, breath analysis is a non-invasive diagnostic method, which has the advantages of non-invasiveness, low cost, simplicity, safety and the like. The study showed that: human breath contains 3000 or more volatile organic gases (VOCs). When the function of human organs or tissues is changed due to damage or lesion, the concentrations of a plurality of metabolic VOCs are correspondingly changed, and the degree of organ damage and lesion can be diagnosed by checking the concentrations of the expiratory VOCs.

Research has shown that the disease development of liver patients is closely related to the concentration of VOCs gas with various physiological and metabolic characteristics (such as isoprene, carbon disulfide, ethane and the like). Especially, the isoprene gas has obvious diagnosis effect on the end-stage liver diseases and has obvious statistical significance. Isoprene is a reactive aliphatic hydrocarbon produced endogenously by humans. It is the major hydrocarbon found in human breath, and is considered a byproduct of cholesterol synthesis and therefore also serves as a marker for cholesterol biosynthesis. In patients with advanced liver fibrosis, the synthetic pathway may be compromised, and this low plasma cholesterol level is characteristic of patients with advanced liver disease. It follows that by detecting the concentration levels of these characteristic gases emitted by the expired breath, a graded diagnosis of liver disease can be made early and quickly.

Non-alcoholic fatty liver disease is exemplified. Generally, about 20-30% of normal people have nonalcoholic fatty liver disease, about 7-30% of the normal people can develop nonalcoholic fatty hepatitis, and about 10-20% of the hepatitis can develop liver fibrosis till cirrhosis and liver cancer. If the accurate typing detection of the liver diseases can be carried out in the early stage, the early intervention can be carried out on high-risk patients in time, and the early discovery and treatment can be realized. Liver biopsy is currently the gold standard for clinical grading of liver fibrosis. The invasive surgery has large trauma, high cost and complex operation, and is not suitable for being used as a detection means for screening the liver diseases of people in a large range.

The currently common exhalation analysis methods mainly comprise gas chromatography-mass spectrometry (GC-MS), proton transfer reaction mass spectrometry (PTR-MS), Ion Mobility Spectrometry (IMS) and other technologies. However, these methods all have the problems of high instrument cost, complicated operation, long analysis time and the like, and are not favorable for large-scale low-cost and rapid screening for serious chronic liver diseases. Therefore, the research of the noninvasive, low-cost, rapid and portable liver disease expiration detection equipment has important significance in the field of early prevention and treatment of liver diseases, and has very wide application prospect in the field of more perfect intelligent medical treatment in the future.

Disclosure of Invention

Based on the combination of the gas chromatography technology and the neural network pattern recognition algorithm, the invention can accurately detect the type and concentration characteristics of the characteristic VOCs gas and screen and diagnose the damage and pathological change degree of human organs. The method has the greatest characteristics of low detection threshold, low cost, no pain, simplicity, safety, portability, low power consumption and the like, and is very suitable for primary screening of major disease hidden dangers for large-scale people. The invention can be used for non-invasive liver disease expiration detection, has low cost and can quickly detect serious chronic liver diseases.

The invention is realized by the following technical scheme.

The invention provides a portable detection device for rapid screening of liver diseases, which comprises:

the sampling bag is used for containing sample gas and is communicated with the micro electromagnetic valve integrated module;

the gas carrying bottle is used for carrying inert gas and is communicated with the micro electromagnetic valve integrated module;

the micro electromagnetic valve integration module is used for enabling the sample gas and the inert gas flowing through the micro channel to respectively flow through the enricher and the chromatographic column, and enabling the sample gas and the inert gas to enter the enricher or the chromatographic column after channel switching is carried out;

the enricher is used for realizing the adsorption and heating desorption of the sample gas;

the chromatographic column is used for separating the gas with complex characteristics in the sample gas on a time axis;

the sensing unit is used for sensing the sample gas entering the unit, extracting response electric signals and transmitting the acquired response information of the liver disease characteristic gases with different degrees to the single chip microcomputer unit;

the single chip microcomputer unit is used for controlling the opening and closing of the flow control modules of the sampling bag and the gas carrying bottle, the miniature electromagnetic valve and the vacuum pump; realizing the temperature closed-loop control of the heating/temperature measuring modules of the enriching device and the chromatographic column; power supply to the sensing unit and acquisition and processing of sensing signals;

the sampling bag and the gas carrying bottle are respectively communicated with the miniature electromagnetic valve integrated module, the miniature electromagnetic valve integrated module is respectively communicated with the enricher, the chromatographic column and the vacuum pump, the chromatographic column is communicated with the sensing unit, and the sensing unit, the vacuum pump, the chromatographic column, the enricher, the flow control module and the miniature electromagnetic valve integrated module are respectively connected with the single chip microcomputer unit.

In the above scheme, a channel installation block and a plurality of micro electromagnetic valves are arranged in the micro electromagnetic valve integration module, the channel installation block is a micro channel arranged on a polymer material, and the plurality of micro electromagnetic valves are respectively fixed on the channel installation block.

In the scheme, the sampling bag and the gas carrying bottle are respectively communicated to the micro electromagnetic valve integration module through the flow control module.

In the scheme, the enrichment device contains a micro-nano adsorption material and is provided with a heating/temperature measuring module.

In the scheme, the inner wall of the chromatographic column is coated with a nano stationary phase material and is provided with a heating/temperature measuring module.

In the above scheme, the sensing unit includes a plurality of metal oxide sensors and a humidity sensor.

In the scheme, the sensing unit is connected to the singlechip unit through the signal processing circuit; the single chip microcomputer unit is connected with a display and data processing module.

The invention further provides a portable detection method for rapid screening of liver diseases based on the equipment, which comprises the following steps:

a gas sampling stage: the vacuum pump is started, sample gas in the sampling bag enters the enricher through the first flow control module and the micro electromagnetic valve integrated module to realize sample gas adsorption, and residual gas flows through the micro electromagnetic valve integrated module and is discharged from the outlet of the vacuum pump;

desorption and sample introduction stage: the enricher heats and desorbs the sample gas, the carrier gas flows through the microchannel of the miniature electromagnetic valve integrated module through the second flow control module, enters the enricher and pushes the desorbed sample gas to flow out of the enricher, and flows through the microchannel of the miniature electromagnetic valve integrated module again and then flows to the chromatographic column, the sample gas and the chromatographic column are fully acted, and the residual gas is discharged along the chromatographic column and the sensing unit in sequence;

a separation and cleaning stage: after the sample gas fully acts with the chromatographic column stationary phase, carrier gas with constant flow flows through the microchannel of the micro electromagnetic valve integrated module and enters the chromatographic column, all components of the separated sample gas sequentially flow out of the chromatographic column to the sensing unit, and the inductive electric signals of all sensors are transmitted to the single chip microcomputer unit; the first carrier gas is directly communicated until no sample gas is separated out from the chromatographic column;

an enrichment cleaning stage: and the carrier gas with constant flow directly enters the enricher through the micro electromagnetic valve integrated module microchannel, cleans the residual gas in the enricher, then flows through the micro electromagnetic valve integrated module microchannel, and is discharged from the outlet of the vacuum pump.

The liver disease rapid screening equipment based on expiration detection has the characteristics of intellectualization, low detection cost, simplicity, safety, short detection time, portability and the like, and can be used for primary disease screening in wider occasions such as families, communities, clinics and the like. On the basis, the patient can be pertinently examined and treated in a professional hospital according to the detection result, so that the trauma and pain of the biopsy to the body of the patient can be reduced as much as possible, unnecessary economic waste can be avoided to a great extent, the shortage of professional medical equipment resources is relieved, and the detection efficiency is improved.

Drawings

FIG. 1 is a schematic block diagram of a system of a portable testing device for rapid screening of liver diseases according to the present invention;

FIG. 2 is a schematic diagram of the operation of the gas sampling stage of the apparatus of the present invention;

FIG. 3 is a schematic diagram of the desorption and sample injection stage of the apparatus of the present invention;

FIG. 4 is a schematic diagram of the operation of the separation purge stage of the apparatus of the present invention;

FIG. 5 is a schematic diagram of the operation of the enrichment wash stage of the apparatus of the present invention.

In the figure: 1. a sampling bag; 2. a first flow control module; 3. a first heating/temperature measuring module; 4. an enricher; 5. a second heating/temperature measuring module; 6. a chromatographic column; 7. a package housing; 8. a sensing unit; 9. a metal oxide sensor; 10. a humidity sensor; 11. a vacuum pump; 12. a miniature electromagnetic valve; 13. a micro electromagnetic valve integration module; 14. a second flow control module; 15. a gas-carrying cylinder; 16. a single chip unit;

17. a signal processing circuit; 18. a display and data processing module; 19. and a channel mounting block.

Detailed Description

The invention is further described in detail below with reference to the drawings and examples, but the invention is not limited thereto.

As shown in fig. 1, the embodiment of the present invention provides a portable detection device for rapid screening of liver diseases, which includes a sampling bag 1, a gas carrying bottle 15, a micro electromagnetic valve integration module 13, an enricher 4, a chromatographic column 6, a sensing unit 8, a vacuum pump 11, a single chip microcomputer unit 16, a signal processing circuit 17, a display and data processing module 18, and the like. The micro electromagnetic valve integrated module 13 is respectively communicated with the sampling bag 1 and the gas carrying bottle 15, the sampling bag 1 is communicated to the micro electromagnetic valve integrated module 13 through the first flow control module 2, and the gas carrying bottle 15 is communicated to the micro electromagnetic valve integrated module 13 through the second flow control module 14.

The micro electromagnetic valve integrated module 13 is further connected with the enricher 4, the chromatographic column 6 and the vacuum pump 11 respectively, the enricher 4 is provided with a first heating/temperature measuring module 3 and two interfaces, and the enricher 4 is connected in parallel with the interface a and the interface c of the micro electromagnetic valve integrated module 13 through the interface A and the interface B; the chromatographic column 6 is provided with a second heating/temperature measuring module 5 and two interfaces, the chromatographic column 6 is connected with the interface b of the micro electromagnetic valve integrated module 13 through the interface C, and the interface D at the other end is connected to the shell inlet of the sensing unit 8. The vacuum pump 11 is connected with the interface d of the micro electromagnetic valve integrated module 13 through a pump inlet, and the other end is connected with the external air environment. The first flow control module 2, the second flow control module 14, the micro electromagnetic valve integration module 13, the enricher 4, the chromatographic column 6, the vacuum pump 11, the signal processing circuit 17 and the sensing unit 8 are all connected with the single chip microcomputer unit 16, and the single chip microcomputer unit 16 is connected with the display and data processing module 18.

Wherein, sampling bag 1 is used for holding the sample gas, sampling bag 1 then selects one kind be applicable to the sampling of low concentration (sub ppm level) volatile organic compounds gas (VOCs), the polymer bag that stability is good such as the gaseous sampling bag of teflon, first flow control module 2 is used for the flow of accurate control appearance of advancing. The carrier gas cylinder 15 is used to contain inert gas, typically selected from nitrogen, and the second flow control module 14 is used to precisely control the flow of the carrier gas. The micro electromagnetic valve integrated module 13 is composed of a channel mounting block 19 containing micro channels and made of polymer materials and a plurality of micro electromagnetic valves 12, and the micro electromagnetic valves are respectively fixed on the channel mounting block 19 and used for switching flow paths. The channel mounting block 19 is formed by an integral molding technique and contains micro-sized channels for the passage of gas.

The enricher 4 contains a micro-nano adsorption material and is used for concentrating and extracting trace characteristic gas to realize adsorption and heating desorption of sample gas. The first heating/temperature measuring module 3 is used for accurately controlling the working temperature of the enricher 4 to complete desorption and cleaning. The inner wall of the chromatographic column 6 is coated with a nano stationary phase material for realizing the separation of the gas with complex characteristics on a time axis; the second heating/temperature measuring module 5 is used for accurately controlling the working temperature of the chromatographic column 6. The sensing unit 8 is composed of a plurality of metal oxide sensors 9 with different selectivity and different sensitivity and a humidity sensor 10, and is packaged in a small-volume packaging shell 7, a shell outlet for exhausting is reserved on the packaging shell 7, wherein the plurality of metal oxide sensors 9 are used for detecting and identifying the characteristic gas of the disease by adopting a neural network mode identification method, and the humidity sensor 10 is used for carrying out humidity compensation on a sensor signal so as to improve the detection accuracy. All the metal oxide sensors 9 and the humidity sensors 10 of the sensing unit 8 are encapsulated in a small-volume encapsulating shell 7, and gas enters from the inlet of the encapsulating shell 7, is sensed by sensitive materials and then is discharged from the outlet of the encapsulating shell 7. The sample gas enters the sensing unit 8 and contacts with the sensitive electrodes of the sensors to generate corresponding sensing signals, and the sensing signals of the sensing unit 8 are denoised, filtered and subjected to analog-to-digital conversion by the signal processing circuit 17 and then input to the single chip microcomputer unit 16. The single chip microcomputer unit 16 extracts the characteristics of the processed induction signals by adopting a characteristic extraction method, and then rapidly screens and diagnoses liver diseases by using a mode identification algorithm according to the fingerprint characteristics of the liver disease characteristic gases with different degrees.

The single chip microcomputer unit 16 loads analog signals to each execution element, specifically controls the opening degree of the flow control module, the opening and closing of the micro electromagnetic valve 12 and the opening and closing of the vacuum pump 11; the temperature closed-loop control of each heating/temperature measuring module of the enriching device 4 and the chromatographic column 6 is realized; the power supply of each sensor of the sensing unit 8 and the acquisition and processing of the sensing signal are realized; signal transmission and interaction with the display and data processing module 18 are realized.

The vacuum pump 11 is connected to the port d of the channel mounting block 19 for providing a system negative pressure.

When the device works, the singlechip microcomputer unit 16 sends out control signals to realize the power supply and control of the first flow control module 2, the second flow control module 14, the miniature electromagnetic valve 12, the first heating/temperature measuring module 3, the second heating/temperature measuring module 5, the sensing unit 8 and the vacuum pump 11, wherein the closed-loop temperature control is realized on the first heating/temperature measuring module 3 and the second heating/temperature measuring module 5. The signal processing circuit 17 performs denoising, filtering and analog-to-digital conversion on the response signal of the sensing unit 8, and then sends the response signal to the single chip microcomputer unit 16, and the single chip microcomputer unit 16 sends the digital signal processed by the algorithm to the display and data processing module 18 for display or manual data analysis and processing. The display and data processing module 18 is used for displaying the result of the acquired data, post-processing the data, and controlling and interacting the parameters of each execution element.

The detection method of the portable detection equipment for rapid screening of liver diseases, which is disclosed by the invention, is shown in fig. 2, fig. 3, fig. 4 and fig. 5, and comprises the following steps:

1) a gas sampling stage: as shown in fig. 2, the sampling bag 1 is connected to the inlet of the first flow control module 2, the vacuum pump 11 is turned on, the first flow control module 2 is responsible for keeping a constant sample introduction flow, the sample gas passes through the micro electromagnetic valve integrated module 13 according to a certain flow path under the action of the vacuum force, enters the enricher 4 to realize gas adsorption, and the residual gas flows through the micro electromagnetic valve integrated module 13 again and is discharged from the outlet of the vacuum pump 11.

2) Desorption and sample introduction stage: as shown in fig. 3, the enricher 4 is heated for desorption, the carrier gas flows through the microchannel of the micro electromagnetic valve integrated module 13 under the constant flow restriction of the second flow control module 14, enters from the interface B of the enricher 4, pushes the desorption gas to flow out from the interface a of the enricher 4, flows through the microchannel of the micro electromagnetic valve integrated module 13 again, flows out from the interface B of the microchannel to the interface C of the chromatographic column 6, the sample gas and the stationary phase of the chromatographic column 6 fully react, and then the residual gas is discharged along the interface D of the chromatographic column 6 and the shell outlet of the sensing unit 8 in sequence.

3) A separation and cleaning stage: as shown in fig. 4, after the sample gas fully acts on the stationary phase of the chromatographic column 6, the carrier gas with a constant flow passes through the microchannel of the micro electromagnetic valve integrated module 13 and directly enters from the interface C of the chromatographic column 6, the components of the separated sample gas sequentially flow out from the interface D of the chromatographic column 6 to the sensing unit 8, each sensor fully senses the sample gas to generate a corresponding electrical signal reflecting the state of the sensor, the sensed electrical signal of each sensor is transmitted to the single chip unit 16, and the rapid screening diagnosis of the liver disease is performed by using the mode recognition algorithm in the single chip unit 16; the carrier gas is passed until no more sample gas is evolved from the column 6.

4) An enrichment cleaning stage: as shown in fig. 5, in preparation for the next test cycle, a constant flow of carrier gas flows through the microchannel of the integrated module of micro solenoid valve 13, directly enters from the port a of the enricher 4, cleans the gas remaining in the enricher 4, then flows out from the port B of the enricher 4, flows through the microchannel of the integrated module of micro solenoid valve 13, and is then discharged from the outlet of the pump 11.

The above process realizes induction and feature extraction of the entered sample gas, and rapid screening and diagnosis of liver diseases are performed by using a pattern recognition algorithm in the single chip microcomputer unit 16 according to the fingerprint features of liver disease feature gases of different degrees.

The present invention is not limited to the above embodiments, and any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention are still within the protection scope of the technical solution of the present invention on the basis of the technical solution disclosed by the present invention.