阅读说明：本技术 一种从低值海洋生物下脚料中提取活性肽的方法 (Method for extracting active peptide from low-value marine organism leftovers ) 是由 房文涛 任海涛 张金秋 刘泓材 于 2020-04-03 设计创作，主要内容包括：本发明公开一种从低值海洋生物下脚料中提取活性肽的方法,包括以下步骤：(1)将低值海洋生物下脚料清洗后加入水,打浆制备得到原料液；(2)在步骤(1)制得的原料液中接种类干酪乳杆菌,进行有氧发酵,制得发酵液；(3)将步骤(2)制得的发酵液离心分离得到沉淀物和上清液,将沉淀物中加入水,重复操作步骤(2)；(4)重复操作步骤(3),将每次离心得到的上清液混合得终发酵物；(5)将步骤(4)得到的终发酵物通过超滤膜膜分离,透过液蒸发浓缩,冷冻干燥得到活性肽。通过本发明提取方法制得的活性肽,活性肽产率高,不仅具有降尿酸的作用,还能够有效治疗乳腺增生疾病。(The invention discloses a method for extracting active peptide from low-value marine organism leftovers, which comprises the following steps: (1) washing the low-value marine organism leftovers, adding water, and pulping to prepare a raw material liquid; (2) inoculating lactobacillus casei to the raw material liquid prepared in the step (1), and carrying out aerobic fermentation to prepare fermentation liquid; (3) centrifuging the fermentation liquor prepared in the step (2) to obtain a precipitate and a supernatant, adding water into the precipitate, and repeating the step (2); (4) repeating the operation step (3), and mixing the supernatant obtained by each centrifugation to obtain a final fermented product; (5) and (4) separating the final fermentation product obtained in the step (4) by an ultrafiltration membrane, evaporating and concentrating the permeate, and freeze-drying to obtain the active peptide. The active peptide prepared by the extraction method has high yield, not only has the function of reducing uric acid, but also can effectively treat hyperplasia of mammary glands.)

1. A method for extracting active peptide from low-value marine organism leftovers is characterized by comprising the following steps:

(1) washing the low-value marine organism leftovers, adding water, and pulping to prepare a raw material liquid with the mass concentration of 10% -15%;

(2) inoculating lactobacillus casei into the raw material liquid prepared in the step (1), and performing aerobic fermentation for 2-3 days at the temperature of 20-30 ℃ to prepare fermentation liquid;

(3) centrifuging the fermentation liquor prepared in the step (2) to obtain a precipitate and a supernatant, adding water into the precipitate, and repeating the step (2);

(4) repeating the step (3), realizing circulating fermentation, and mixing the supernatant obtained by each centrifugation to obtain a final fermented product;

(5) and (4) separating the final fermentation product obtained in the step (4) by an ultrafiltration membrane to obtain a permeate, evaporating and concentrating the permeate, and freeze-drying to obtain the active peptide.

2. The method for extracting active peptides of claim 1, wherein: in the step (2), the inoculation amount of the lactobacillus paracasei is 10⁵-10⁶CFU/mL。

3. The method for extracting active peptides of claim 2, wherein: the lactobacillus paracasei is preserved by China Industrial microorganism culture Collection, the strain numbers are CICC20241 and CICC6270, and the inoculation proportion of the CICC20241 and the CICC6270 is 1 (2-4).

4. The method for extracting active peptides of claim 1, wherein: in the step (3), the solid-to-liquid ratio of the precipitate to water is 1g (4-5) ml.

5. The method for extracting active peptides of claim 1, wherein: in the step (5), the membrane flux of the ultrafiltration membrane is 5000 Da.

6. The method for extracting active peptides of claim 1, wherein: in the step (5), before membrane separation, centrifugal impurity removal is carried out, and the centrifugal force is 8000-10000 g.

7. An active peptide extracted by the method of any one of claims 1 to 6.

8. Use of the active peptide of claim 7 in the manufacture of a medicament for the treatment of mammary gland hyperplasia.

9. A medicament for the treatment of hyperplasia of mammary glands, characterized by: the agent comprises the active peptide according to claim 7 as an active ingredient.

Technical Field

The invention relates to the technical field of recycling and extracting active peptide from low-value marine organism leftovers, in particular to a method for extracting active peptide from low-value marine organism leftovers.

Background

In the marine organism processing process, marine organism leftovers such as fish, squid viscera and the like are generally not easy to be utilized and are directly discarded, so that on one hand, the waste of resources is caused, and on the other hand, the environmental pollution is caused.

The hyperplasia of mammary glands is the retrograde and progressive change of the structure of a mammary duct and lobules, takes breast pain and breast lumps as main clinical manifestations, is the most common mammary gland disease of women of childbearing age, seriously influences the life quality of patients, and is generally treated by hormones and vitamins in the existing treatment mode, but the curative effect is not exact, the side effect is large, and the treatment effect is poor. At present, no low-value marine organism leftovers are applied to relevant research on prevention and treatment of hyperplasia of mammary glands, and the active peptide extracted by the method has an important effect on prevention and treatment of hyperplasia of mammary glands after research.

Disclosure of Invention

In view of the prior art, the invention aims to provide a method for extracting active peptide from low-value marine organism leftovers. The active peptide is extracted according to the method, the yield of the active peptide is high, and the extracted active peptide not only has the function of reducing uric acid, but also can effectively treat hyperplasia of mammary glands; the invention reuses the low-value marine organism leftovers, avoids the waste of the leftovers and develops a new way for utilizing the low-value marine organism leftovers.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect of the invention, a method for extracting active peptide from low-value marine organism leftovers is provided, which comprises the following steps:

(1) washing the low-value marine organism leftovers, adding water, and pulping to prepare a raw material liquid with the mass concentration of 10% -15%;

(2) inoculating lactobacillus casei into the raw material liquid prepared in the step (1), and performing aerobic fermentation for 2-3 days at the temperature of 20-30 ℃ to prepare fermentation liquid;

(3) centrifuging the fermentation liquor prepared in the step (2) to obtain a precipitate and a supernatant, adding water into the precipitate, and repeating the step (2);

(4) repeating the step (3), realizing circulating fermentation, and mixing the supernatant obtained by each centrifugation to obtain a final fermented product;

(5) and (4) separating the final fermentation product obtained in the step (4) by an ultrafiltration membrane to obtain a permeate, evaporating and concentrating the permeate, and freeze-drying to obtain the active peptide.

Preferably, the low-value marine organism leftovers are viscera of fishes and squids.

Preferably, in the step (2), the inoculation amount of the lactobacillus paracasei is 10⁵-10⁶CFU/mL。

Preferably, the lactobacillus paracasei is deposited by China Industrial microorganism culture Collection, the strain numbers are CICC20241 and CICC6270, and the inoculation ratio of the CICC20241 and the CICC6270 is 1 (2-4).

Preferably, in the step (3), the solid-to-liquid ratio of the precipitate to the water is 1g (4-5) ml.

Preferably, in the step (4), the operation of the step (3) is repeated 3 to 4 times.

Preferably, in step (5), the membrane flux of the ultrafiltration membrane is 5000 Da.

Preferably, in the step (5), centrifugal impurity removal is performed before membrane separation, and the centrifugal force is 8000-10000 g.

In a second aspect of the present invention, the active peptide is extracted by the above method.

In a third aspect of the invention, the application of the active peptide in preparing a medicament for treating hyperplasia of mammary glands is provided.

In a fourth aspect of the present invention, there is provided a pharmaceutical agent for treating mammary gland hyperplasia, which comprises the above-mentioned active peptide as an active ingredient.

The invention has the beneficial effects that:

the active peptide is extracted according to the method, the yield of the active peptide is high, and the extracted active peptide not only has the function of reducing uric acid, but also can effectively treat hyperplasia of mammary glands; the invention reuses the low-value marine organism leftovers, avoids the waste of the leftovers and develops a new way for utilizing the low-value marine organism leftovers; wide raw material source, low price, low production cost, simple production process and suitability for various scale production.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

As introduced in the background art, the utilization effect of the marine organism leftovers is poor and the yield of the active peptides is low. Based on the above, the active peptide is extracted by the extraction method, the yield of the active peptide is high, and the extracted active peptide not only has the effect of reducing uric acid, but also can effectively treat hyperplasia of mammary glands; the invention reuses the low-value marine organism leftovers, avoids the waste of the leftovers and develops a new way for utilizing the low-value marine organism leftovers.

In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.

The test materials used in the examples and comparative examples of the present invention are conventional in the art and are commercially available.