阅读说明：本技术 一种prpp的定量检测方法 (Quantitative detection method of PRPP ) 是由 戴柱 宋锦华 于 2018-01-22 设计创作，主要内容包括：一种PRPP的定量检测方法,涉及特定物质的检测方法的技术领域。该方法采用HPLC进行检测,色谱条件如下：色谱柱以三唑基键合硅胶为填料,流动相由A相和B相组成,A相为pH6.0-6.8的缓冲液,B相为极性4-7、能与水互溶且能溶解PRPP的溶剂,A相在流动相中的体积百分比为35-50%,采用示差检测器进行检测。(A PRPP quantitative detection method relates to the technical field of detection methods of specific substances. The method adopts HPLC for detection, and the chromatographic conditions are as follows: the chromatographic column uses triazolyl bonded silica gel as a filler, a mobile phase consists of a phase A and a phase B, the phase A is a buffer solution with the pH value of 6.0-6.8, the phase B is a solvent which has the polarity of 4-7, can be mutually dissolved with water and can dissolve PRPP, the volume percentage of the phase A in the mobile phase is 35-50%, and a differential detector is adopted for detection.)