用于检测巴贝虫的组合物和方法

文档序号:1590671 发布日期:2020-01-03 浏览:34次 >En<

阅读说明:本技术 用于检测巴贝虫的组合物和方法 (Compositions and methods for detecting babesia ) 是由 E·H·费斯 J·孙 于 2018-07-18 设计创作,主要内容包括:描述了用于快速检测生物学或非生物学样品中巴贝虫(Babesia)的存在或缺失的方法。该方法可包括实施扩增步骤,杂交步骤,和检测步骤。而且,提供了靶向巴贝虫的18S基因的引物和探针,和试剂盒,它们设计用于检测巴贝虫,包括但不限于田鼠巴贝虫(B.microti),分歧巴贝虫(B.divergens),邓肯巴贝虫(B.duncani),和猎户巴贝虫(B.venatorum)的巴贝虫属物种。还描述的是用于扩增和检测巴贝虫的试剂盒,反应混合物,和寡核苷酸(例如引物和探针)。(Methods for rapidly detecting the presence or absence of Babesia (Babesia) in a biological or non-biological sample are described. The method may comprise performing an amplification step, a hybridization step, and a detection step. Furthermore, primers and probes targeting the 18S gene of babesia, and kits designed for the detection of babesia species, including but not limited to babesia microti (b.microti), babesia divergens (b.divergens), babesia dunken (b.dunnani), and babesia hunter (b.venatorum), are provided. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primers and probes) for amplifying and detecting babesia.)

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