阅读说明：本技术 一种漆酶合成聚吡咯的方法 (Method for synthesizing polypyrrole by laccase ) 是由 杨智钧 王锦锦 刘丹丹 吕爱平 于 2019-11-05 设计创作，主要内容包括：本发明属于酶催化技术领域,更具体的涉及一种漆酶合成聚吡咯的方法。本发明采用漆酶作为催化剂,将吡咯、聚乙二醇、pH＝3.0的醋酸缓冲溶液、漆酶粗酶液和1-羟基苯并三唑置于水溶液中,室温反应即得。本发明较化学方法相比无需额外氧化剂,且反应条件更为温和,绿色无污染。(The invention belongs to the technical field of enzyme catalysis, and particularly relates to a method for synthesizing polypyrrole by laccase. The invention adopts laccase as a catalyst, and the preparation method comprises the steps of putting pyrrole, polyethylene glycol, acetic acid buffer solution with pH value of 3.0, crude laccase solution and 1-hydroxybenzotriazole into an aqueous solution, and reacting at room temperature to obtain the product. Compared with a chemical method, the method does not need additional oxidant, has mild reaction conditions, and is green and pollution-free.)

1. A method for synthesizing polypyrrole by laccase is characterized in that: putting pyrrole, polyethylene glycol, acetic acid buffer solution with pH of 3.0, crude laccase solution and 1-hydroxybenzotriazole into an aqueous solution, and reacting at room temperature to obtain the product.

2. The method of claim 1, wherein: the concentration of pyrrole in the aqueous solution is 10mmol/L, the concentration of PEG is 2.5-20mmol/L, and the concentration of 1-hydroxybenzotriazole is 0.05 mg/ml; the PEG is PEG2000, PEG4000 or PEG 6000.

3. The method of claim 1, wherein: the crude laccase enzyme solution is from Ganoderma.

4. The method of claim 3, wherein: the crude laccase enzyme solution is derived from sweet lucid ganoderma of lucid ganoderma.

5. The method of claim 4, wherein: the PEG concentration is 15 mmol/L.

Technical Field

The invention belongs to the technical field of enzyme catalysis, and particularly relates to a method for synthesizing polypyrrole by laccase.

Background

In the research of polymer materials, polypyrrole has been recognized as one of the most potential polymer materials. The existing research shows that the polypyrrole film plays a role in shielding and passivating metal, the polypyrrole has higher conductivity and good stability in the environment, the polypyrrole is an ideal electrode material of a polymer secondary battery, the polypyrrole also has unique doping performance, the polypyrrole can be modified to a certain degree, the polypyrrole can be well applied in the aspect of electrocatalysis, and the polypyrrole also has good biocompatibility, so the polypyrrole can also be applied in the biological field in a large scale.

The existing methods for synthesizing polypyrrole mainly comprise: (1) ammonium persulfate method; (2) a ferric chloride process; and (3) a hydrogen peroxide process. The three methods are chemical catalytic polymerization methods, and the enzyme catalysis is milder in reaction conditions and cleaner in reaction compared with the chemical catalytic method.

Laccase can catalyze the oxidation reaction of various compounds, the substrates are wide, but the catalytic activity difference of laccase from different sources is large; at present, no laccase is reported to catalyze the polymerization of pyrrole to generate polypyrrole.

Disclosure of Invention

The invention aims to provide a method for preparing polypyrrole by enzyme catalysis of pyrrole, in particular to a method for preparing polypyrrole by catalyzing pyrrole with laccase.

The method for synthesizing polypyrrole by laccase is characterized in that pyrrole, polyethylene glycol, acetic acid buffer solution with pH value of 3.0, laccase crude enzyme solution and 1-Hydroxybenzotriazole (HBT) are placed in aqueous solution and react at room temperature to obtain the polypyrrole under the condition of pH value of 3.0.

Preferably, the concentration of pyrrole in the aqueous solution is 10mmol/L, the concentration of PEG is 2.5-20mmol/L, and the concentration of 1-hydroxybenzotriazole is 0.05 mg/ml; the PEG is PEG2000, PEG4000 or PEG 6000;

preferably, the crude laccase enzyme solution is from ganoderma, and the crude laccase enzyme solution source is further preferably sweet ganoderma of ganoderma;

preferably, the concentration of the PEG is 15 mmol/L; the experimental result shows that different PEG concentrations have obvious influence on the pyrrole polymerization, and when the PEG concentration is 15mmol/L, the absorbance of the reaction liquid is maximum between 400 and 500nm, which indicates that the pyrrole polymerization effect is best under the conditions.

Compared with the prior art, the invention has the following advantages:

(1) according to the invention, laccase is used as a catalyst for catalyzing polymerization of pyrrole to prepare polypyrrole for the first time, no additional oxidant is needed, and the reaction conditions are milder.

(2) The invention discovers that when laccase catalyzes pyrrole polymerization, the concentration of PEG has a great influence on the polymerization, and the catalysis effect is best when the concentration of PEG is 15 mmol/L.

Drawings

FIG. 1 is a UV full-wavelength scanning chart of a reaction solution after the completion of the reaction in example 1.

FIG. 2 is a UV full-wavelength scanning chart of the reaction solution after the reaction is finished under different PEG concentrations in example 2.

Detailed Description