阅读说明：本技术 一种从胰渣中提取饲料级胰酶的制备工艺及其制备方法 (Preparation process and preparation method for extracting feed-grade pancreatin from pancreatin residues ) 是由 蔡青和 朱琳娜 于 2019-07-04 设计创作，主要内容包括：本发明公开的属于胰酶提取技术领域,具体为一种从胰渣中提取饲料级胰酶的制备工艺,该从胰渣中提取饲料级胰酶的制备工艺步骤如下：步骤一：原料处理：原料为粗酶粉提取血管紧张素后的冷冻胰渣,原料用蒸馏水溶解,用平板粉碎机或刨片机将原料进行粉碎,再用搅拌机搅拌15-30分钟溶解,或者用磨浆机磨浆溶解；步骤二：离心：用离心机离心或者分离机过滤除渣,得到上清滤液；步骤三：沉淀、浓缩：在上清滤液中加入浓度为60％-100％乙醇,搅拌均匀后沉淀后,该发明具有污染小、设备简单、生产周期短、无需添加激活剂、性质稳定便于保存,加入饲料中饲喂畜禽可显著改善动物生产性能或者提高饲料原料利用率的特点。(The invention belongs to the technical field of pancreatin extraction, and particularly relates to a preparation process for extracting feed-grade pancreatin from pancreatin residues, which comprises the following steps: the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, the raw material is dissolved by distilled water, the raw material is crushed by a flat plate crusher or a flaker, and then is stirred by a stirrer for 15-30 minutes to be dissolved, or is ground and dissolved by a pulping machine; step two: centrifuging: centrifuging with a centrifuge or filtering with a separator to remove residue to obtain supernatant filtrate; step three: and (3) precipitation and concentration: the invention has the characteristics of small pollution, simple equipment, short production period, no need of adding an activating agent, stable property and convenient storage after adding 60-100% ethanol into the supernatant filtrate and uniformly stirring and then precipitating, and can obviously improve the production performance of animals or improve the utilization rate of feed raw materials when being added into feed for feeding livestock and poultry.)

1. A preparation process for extracting feed-grade pancreatin from pancreatin residues is characterized in that: the preparation process for extracting the feed-grade pancreatin from the pancreatin residue comprises the following steps:

the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, the raw material is crushed by a flat plate crusher or a flaker, dissolved by distilled water and stirred by a stirrer for 15-30 minutes, or dissolved by pulping by a pulping machine;

step two: centrifuging: centrifuging with a centrifuge or filtering with a separator to remove residue to obtain supernatant filtrate;

step three: and (3) precipitation and concentration: adding 60% -100% ethanol into the supernatant filtrate, stirring, precipitating at 10-20 deg.C for 0.5-2.5 hr, removing supernatant, and collecting precipitate;

step four: drying and granulating: mixing the precipitate obtained in the third step with a carrier for feed, and drying by using a drying device or drying after granulating to obtain pancreatin;

step five: detecting and packaging: the pancreatin comprises trypsin, pancrelipase and pancreatin, the trypsin is more than or equal to 1000u/g, the pancreatin is more than or equal to 500u/g, the activity of the pancrelipase is more than or equal to 800u/g, the water content is less than or equal to 6 percent, the storage period is more than or equal to 12 months, and the pancreatin is packaged after being detected to be qualified.

2. The process of claim 1 for the extraction of feed-grade pancreatin from pancreatin pomace, wherein: the carrier for the feed is any one of corn starch, silicon dioxide, gelatin and rice hull powder.

3. The process of claim 1 for the extraction of feed-grade pancreatin from pancreatin pomace, wherein: the drying device is air-blast drying, vacuum drying or fluidized bed drying.

4. The process of claim 1 for the extraction of feed-grade pancreatin from pancreatin pomace, wherein: the dissolving temperature is controlled at 10-20 ℃, and the ratio of distilled water to raw materials is controlled at 3-1: 1.

Technical Field

The invention relates to the technical field of pancreatin extraction, in particular to a preparation process for extracting feed-grade pancreatin from the residual pancreatin slag after extracting angiotensin from crude enzyme powder and a preparation method thereof.

Background

The digestive tract of young animals such as piglets, chicks, ducklings, dairy cows and the like is underdeveloped, an endogenous digestive enzyme system is not established, and the young animals are easy to have nutritional diarrhea and diarrhea caused by other reasons due to early weaning and other feeding systems. By adding suitable enzyme preparation, the occurrence of nutritional diarrhea in young animals can be prevented, thereby reducing the use of antibiotics.

Pancreatin, a protein with special functions. Pancreatin in the pharmaceutical industry is mainly used for dyspepsia, inappetence and digestive disorder caused by liver and pancreas diseases, and is also suitable for congenital pancreatic insufficiency, and pancreatic insufficiency caused by abdominal operation and traumatic pancreatic resection. Or pancreatic insufficiency caused by non-surgical excision and chronic pancreatitis caused by alcoholism. Also, pancreatin has a wide range of applications in the feed industry, not only providing a protein source to animals, but also because it contains a large number of biologically active components, such as proteases, amylases and lipases and active peptides.

At present, the traditional preparation process of industrial pancreatin mostly takes pig or ox pancreas as a raw material, and comprises a plurality of procedures of raw material treatment (flaking, grinding into thick liquid), activation, extraction, separation (filtration, precipitation, squeezing), degreasing, granulation, drying, crushing and the like. The raw material for extracting angiotensin for medicine is derived from crude enzyme powder (derived from pig pancreas), and the crude enzyme powder contains angiotensin, trypsin, pancreatic amylase and pancreatic lipase. The process for extracting the angiotensin mainly adopts water washing and specific molecular filter membrane filtration, and the enzyme in the angiotensin is slightly damaged. The pancreatic residues left after the crude enzyme powder extracts the angiotensin for medicine are used as raw materials for preparing the pancreatin, so that the economic value and the feasibility are certain, on one hand, the use of a large amount of organic solvents and the addition of protective agents are avoided, on the other hand, the pancreatic resources are fully and comprehensively utilized, and the method has great significance in the aspects of environmental protection and resource saving. At present, the process for preparing pancreatin by using the pancreatic residues left after the extraction of angiotensin for medicine is not reported yet. Taking the rest pancreas residue after extracting insulin from pancreas as raw material, directly precipitating with PEG-6000 and passing through CaCl₂Activated pancreatic residues and supernatant are subjected to the procedures of centrifugation, acetone precipitation, ether degreasing and the like to prepare pancreatin, but acetone, ether and other dangerous chemicals which are easy to prepare poison and explode are still used in the method, the control difficulty under the production environment of a factory is increased, and PEG-6000 viscosity is highThe operation difficulty of centrifugation, filtration and the like is increased, and the product also carries more PEG, which needs further treatment, so that the development of a preparation process for extracting the feed-grade pancreatin from the pancreatin residue is urgently needed.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above and/or problems occurring in the existing process for preparing feed-grade pancreatin extracted from pancreatic residues.

Therefore, the invention aims to provide a preparation process for extracting feed-grade pancreatin from pancreatin residues, which has the advantages of small pollution, simple equipment, short production period, no need of adding an activating agent, stable property and convenient storage, and can obviously improve the production performance of animals or improve the utilization rate of feed raw materials when added into feed for feeding livestock and poultry.

To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions:

a preparation process for extracting feed-grade pancreatin from pancreatin residues comprises the following steps:

the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, the raw material is crushed by a flat plate crusher or a flaker, dissolved by distilled water and stirred by a stirrer for 15-30 minutes, or ground and dissolved by a pulping machine;

step two: centrifuging: centrifuging with a centrifuge or filtering with a separator to remove residue to obtain supernatant filtrate;

step three: and (3) precipitation and concentration: adding 60% -100% ethanol into the supernatant filtrate, stirring, precipitating at 10-20 deg.C for 0.5-2.5 hr, removing supernatant, and collecting precipitate;

step four: drying and granulating: mixing the precipitate obtained in the third step with a carrier for feed, and drying by using a drying device or drying after granulating to obtain pancreatin;

step five: detecting and packaging: the pancreatin comprises trypsin, pancrelipase and pancreatin, the trypsin is more than or equal to 1000u/g, the pancreatin is more than or equal to 500u/g, the activity of the pancrelipase is more than or equal to 800u/g, the water content is less than or equal to 6 percent, the storage period is more than or equal to 12 months, and the pancreatin is packaged after being detected to be qualified.

As a preferable scheme of the preparation process for extracting the feed-grade pancreatin from the pancreatin residue, the invention comprises the following steps: the carrier for the feed is any one of corn starch, silicon dioxide, gelatin and rice hull powder.

As a preferable scheme of the preparation process for extracting the feed-grade pancreatin from the pancreatin residue, the invention comprises the following steps: the drying device is air-blast drying, vacuum drying or fluidized bed drying.

As a preferable scheme of the preparation process for extracting the feed-grade pancreatin from the pancreatin residue, the invention comprises the following steps: the dissolving temperature is controlled at 10-20 ℃, and the ratio of distilled water to raw materials is controlled at 3-1: 1.

Compared with the prior art: the preparation process for extracting the feed-grade pancreatin from the pancreatic residues has the advantages of small pollution, simple equipment, short production period, no need of adding an activating agent, stable property and convenient storage, and can obviously improve the production performance of animals or improve the utilization rate of feed raw materials and increase the apparent digestibility of nutrient substances without influencing the content of blood metabolites and bacteria in excrement when being added into the feed for feeding livestock and poultry.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and detailed embodiments, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise. Wherein:

FIG. 1 is a flow chart of the production process for extracting feed-grade pancreatin from pancreatin dregs.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and it will be apparent to those of ordinary skill in the art that the present invention may be practiced without departing from the spirit and scope of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.