阅读说明：本技术 一种色谱柱温度控制系统及方法 (Chromatographic column temperature control system and method ) 是由 严鹏 易建 林滔 王翔 史乐 于 2019-10-23 设计创作，主要内容包括：本发明公开一种色谱柱温度控制系统及方法,该系统包括陶瓷发热片、温度测量元件、主控板、驱动电路、功率器件以及电源；所述温度测量元件与主控板连接,用于检测色谱柱温度,并将检测结果反馈给主控板；所述主控板与驱动电路连接,用于计算控制量并输出至驱动电路；所述驱动电路与功率器件连接,用于根据收到的控制量,控制功率器件通断动作,通过功率器件开关电源控制陶瓷发热片的发热量。本发明采用陶瓷发热片和分离器件搭建的驱动结构,去掉了不必要的散热和滤波模块,结构简单,成本低；根据陶瓷发热片良好的温度-阻抗线性关系,在温度反馈控制的基础上,加入了电流反馈控制,提高了控制精度和抗干扰能力,色谱柱温度得到了有效控制。(The invention discloses a chromatographic column temperature control system and a method, wherein the system comprises a ceramic heating plate, a temperature measuring element, a main control board, a driving circuit, a power device and a power supply; the temperature measuring element is connected with the main control board and used for detecting the temperature of the chromatographic column and feeding back a detection result to the main control board; the main control board is connected with the driving circuit and used for calculating control quantity and outputting the control quantity to the driving circuit; the driving circuit is connected with the power device and used for controlling the on-off action of the power device according to the received control quantity and controlling the heat productivity of the ceramic heating sheet through the power device switching power supply. The invention adopts the driving structure built by the ceramic heating sheet and the separating device, removes unnecessary heat dissipation and filtering modules, and has simple structure and low cost; according to the good temperature-impedance linear relation of the ceramic heating sheet, on the basis of temperature feedback control, current feedback control is added, so that the control precision and the anti-interference capability are improved, and the temperature of the chromatographic column is effectively controlled.)

1. A chromatography column temperature control system, comprising: the device comprises a ceramic heating sheet, a temperature measuring element, a main control board, a driving circuit, a power device and a power supply; the ceramic heating sheet is used for heating the chromatographic column; the temperature measuring element is connected with the main control board and used for detecting the temperature of the chromatographic column and feeding back a detection result to the main control board; the main control board is connected with the driving circuit and used for calculating control quantity according to temperature data fed back by the temperature measuring element and outputting the control quantity to the driving circuit; the driving circuit is connected with the power device and used for controlling the on-off action of the power device according to the received control quantity; one end of the power device is connected with the ceramic heating sheet, and the other end of the power device is connected with the power supply and used for controlling the heating amount of the ceramic heating sheet through the power device switching power supply.

2. The chromatographic column temperature control system of claim 1, further comprising a current measuring element connected with the main control board for detecting the real-time current of the ceramic heating plate and feeding back the detection result to the main control board.

3. The system of claim 2, wherein the main control board is specifically configured to calculate a control amount according to data fed back by the temperature measuring element and the current measuring element and output the control amount to the driving circuit, so as to control the heat generation amount of the ceramic heating plate.

4. The chromatographic column temperature control system of claim 1, wherein the main control board is further connected with an upper computer for receiving a temperature control command sent by the upper computer.

5. The system of claim 4, wherein the MCU master control board is used as the master control board and communicates with the upper computer through RS 232.

6. The system of any one of claims 1 to 5, further comprising a temperature controlled chamber for holding the chromatography column.

7. A method for controlling the temperature of a chromatography column based on the system for controlling the temperature of a chromatography column according to claim 1, comprising the steps of:

s101, when the chromatographic column temperature control system is in a standby state at the beginning, waiting for an instruction of an upper computer;

s102, after the upper computer sends a temperature control instruction, the chromatographic column temperature control system is switched to a working state, and the main control board measures a current value and a temperature value through a temperature measuring element and a current measuring element and carries out filtering processing;

s103, after the main control board obtains the processed temperature and current values, calculating a temperature difference value, a temperature accumulated difference value, a temperature difference value gradient and a current difference value gradient;

s104, after the main control board finishes the calculation, weighting each calculated value, outputting a duty ratio signal to a driving circuit, further controlling the on-off action of a power device by the driving circuit, and controlling the heat productivity of the ceramic heating sheet through a power device switching power supply so as to finish the temperature control of the chromatographic column; meanwhile, the main control board records each calculated value at this time and is used when the step S103 is executed;

and S105, the chromatographic column temperature control system waits for an upper computer ending command, if an ending command exists, ending control, and switching to a standby state, and if no ending command exists, returning to the step S102 to continue working.

8. The method for controlling the temperature of a chromatographic column according to claim 7, wherein the main control board adopts an MCU main control board and communicates with an upper computer through RS 232.

Technical Field

The invention relates to the field of high-pressure liquid chromatography, in particular to a chromatographic column temperature control system and a chromatographic column temperature control method.

Background

High pressure liquid chromatography is an important branch of chromatography, and takes liquid as a mobile phase, and a high pressure sample feeding system is used for feeding sample liquid and buffer solution into a chromatographic column. In the chromatographic column, different components in the liquid are separated, flow into a detector, and are subjected to photoelectric conversion, the component values are converted into electric signals, and the electric signals are recorded and stored to form a waveform map, so that the analysis of a sample is realized.

In sample analysis, the environmental temperature influences the test result, and if the temperature fluctuation is overlarge, the accuracy and consistency of the test are greatly influenced. Therefore, the temperature control of the chromatographic column is an important link in high-pressure liquid phase analysis. The existing product is generally provided with a column temperature box to control the temperature of an analysis column, and a used temperature control system is designed based on a TEC semiconductor device. However, due to the characteristics of the TEC semiconductor, an additional filter circuit and a heat dissipation module must be provided, and the device is high in cost, easy to damage, and not suitable for a chromatography system requiring only unidirectional temperature control.

Disclosure of Invention

The present invention aims to solve the problems mentioned in the background section above by means of a system and a method for controlling the temperature of a chromatography column.

In order to achieve the purpose, the invention adopts the following technical scheme:

a chromatography column temperature control system, the system comprising: the device comprises a ceramic heating sheet, a temperature measuring element, a main control board, a driving circuit, a power device and a power supply; the ceramic heating sheet is used for heating the chromatographic column; the temperature measuring element is connected with the main control board and used for detecting the temperature of the chromatographic column and feeding back a detection result to the main control board; the main control board is connected with the driving circuit and used for calculating control quantity according to temperature data fed back by the temperature measuring element and outputting the control quantity to the driving circuit; the driving circuit is connected with the power device and used for controlling the on-off action of the power device according to the received control quantity; one end of the power device is connected with the ceramic heating sheet, and the other end of the power device is connected with the power supply and used for controlling the heating amount of the ceramic heating sheet through the power device switching power supply.

Particularly, the chromatographic column temperature control system further comprises a current measuring element, wherein the current measuring element is connected with the main control board and is used for detecting the real-time current of the ceramic heating sheet and feeding back the detection result to the main control board.

Particularly, the main control board is specifically used for calculating a control quantity according to data fed back by the temperature measuring element and the current measuring element and outputting the control quantity to the driving circuit, so that the control of the heat productivity of the ceramic heating sheet is realized.

Particularly, the main control board is also connected with the upper computer and used for receiving a temperature control instruction sent by the upper computer.

Particularly, the main control board adopts an MCU main control board and communicates with an upper computer through RS 232.

Particularly, the chromatographic column temperature control system further comprises a temperature control box, and the temperature control box is used for placing the chromatographic column.

Based on the chromatographic column temperature control system, the invention also provides a chromatographic column temperature control method, which comprises the following steps:

s101, when the chromatographic column temperature control system is in a standby state at the beginning, waiting for an instruction of an upper computer;

s102, after the upper computer sends a temperature control instruction, the chromatographic column temperature control system is switched to a working state, and the main control board measures a current value and a temperature value through a temperature measuring element and a current measuring element and carries out filtering processing;

s103, after the main control board obtains the processed temperature and current values, calculating a temperature difference value, a temperature accumulated difference value, a temperature difference value gradient and a current difference value gradient;

s104, after the main control board finishes the calculation, weighting each calculated value, outputting a duty ratio signal to a driving circuit, further controlling the on-off action of a power device by the driving circuit, and controlling the heat productivity of the ceramic heating sheet through a power device switching power supply so as to finish the temperature control of the chromatographic column; meanwhile, the main control board records each calculated value at this time and is used when the step S103 is executed;

and S105, the chromatographic column temperature control system waits for an upper computer ending command, if an ending command exists, ending control, and switching to a standby state, and if no ending command exists, returning to the step S102 to continue working.

Particularly, the main control board adopts an MCU main control board and communicates with an upper computer through RS 232.

The chromatographic column temperature control system and the method provided by the invention adopt the driving structure built by the ceramic heating sheet and the separating device, and simultaneously remove unnecessary heat dissipation and filtering modules, so that the structure is simple, and the hardware cost is reduced; according to the invention, current feedback control is added on the basis of temperature feedback control according to the good temperature-impedance linear relation of the ceramic heating sheet, so that the control precision and the anti-interference capability are improved, and the temperature of the chromatographic column is effectively controlled.

Drawings

FIG. 1 is a schematic structural diagram of a temperature control system for a chromatographic column according to an embodiment of the present invention;

fig. 2 is a schematic flow chart of a chromatographic column temperature control method according to an embodiment of the present invention.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete. It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1, fig. 1 is a schematic structural diagram of a chromatographic column temperature control system according to an embodiment of the present invention.

The chromatographic column temperature control system in this embodiment specifically includes: a ceramic heating plate 101, a temperature measuring element 102, a main control board 103, a driving circuit 104, a power device 105 and a power supply 106. The ceramic heating sheet 101 is used for heating the chromatographic column. The temperature measuring element 102 is connected to the main control board 103, and is configured to detect a temperature of the chromatography column and feed back a detection result to the main control board 103. The main control board 103 is connected to the driving circuit 104, and is configured to calculate a control amount according to the temperature data fed back by the temperature measuring element 102 and output the control amount to the driving circuit 104. The driving circuit 104 is connected to the power device 105, and is configured to receive the control quantity, amplify the main control signal, and control on/off operation of the power device 105. One end of the power device 105 is connected with the ceramic heating sheet 101, and the other end is connected with the power supply 106, and the power device 105 is used for switching the power supply 106 to control the heating value of the ceramic heating sheet 101. In this embodiment, the ceramic heating sheet 101 is a high-temperature fired ceramic heating sheet (MCH). The power device 105 is not particularly limited as long as it is a switching device that satisfies the requirements.

Specifically, in this embodiment, the temperature control system for the chromatographic column further includes a current measuring element, the current measuring element is connected to the main control board 103 and configured to detect a real-time current of the ceramic heating sheet 101, and feed back a detection result to the main control board 103, and the main control board 103 calculates a control amount according to data fed back by the temperature measuring element 102 and the current measuring element and outputs the control amount to the driving circuit 104, thereby realizing control of the heating amount of the ceramic heating sheet 101.

Specifically, in this embodiment, the main control board 103 is further connected to the upper computer 107, and is configured to receive a temperature control instruction sent by the upper computer 107. The main control board 103 adopts an MCU main control board 103, and communicates with the upper computer 107 through RS 232. In this embodiment, the system for controlling the temperature of the chromatographic column further includes a temperature control box 108, and the temperature control box 108 has a heat preservation function and is used for placing the chromatographic column.

As shown in fig. 2, fig. 2 is a schematic flow chart of a method for controlling a temperature of a chromatography column according to an embodiment of the present invention. The method for controlling the temperature of the chromatographic column in the embodiment specifically comprises the following steps:

s101, when the chromatographic column temperature control system is in a standby state at the beginning, waiting for an instruction of an upper computer.

S102, after the upper computer sends out a temperature control instruction, the chromatographic column temperature control system is switched to a working state, and the main control board measures a current value and a temperature value through the temperature measuring element and the current measuring element and carries out filtering processing. In this embodiment, the main control board adopts an MCU main control board, and communicates with an upper computer through RS 232.

And S103, after the main control board obtains the processed temperature and current values, calculating a temperature difference value, a temperature accumulated difference value, a temperature difference gradient and a current difference gradient.

S104, after the main control board finishes the calculation, weighting each calculated value, outputting a duty ratio signal to a driving circuit, further controlling the on-off action of a power device by the driving circuit, and controlling the heat productivity of the ceramic heating sheet through a power device switching power supply so as to finish the temperature control of the chromatographic column; meanwhile, the main control board records each calculated value at this time, and the calculated values are used when step S103 is executed.

And S105, the chromatographic column temperature control system waits for an upper computer ending command, if an ending command exists, ending control, and switching to a standby state, and if no ending command exists, returning to the step S102 to continue working.

According to the technical scheme, the ceramic heating plate and the driving structure built by the separating device are adopted, unnecessary heat dissipation and filtering modules are removed, the structure is simple, and the hardware cost is reduced; the invention adopts double closed-loop control of current and temperature, adds current feedback control on the basis of temperature feedback control according to good temperature-impedance linear relation of the ceramic heating sheet, improves control precision and anti-interference capability, effectively controls the temperature of the chromatographic column, and realizes double advantages of cost and performance.

It will be understood by those skilled in the art that all or part of the above embodiments may be implemented by the computer program to instruct the relevant hardware, and the program may be stored in a computer readable storage medium, and when executed, may include the procedures of the embodiments of the methods as described above. The storage medium may be a magnetic disk, an optical disk, a read-only memory or a random access memory.

It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.