阅读说明：本技术 一种祛疤膏及其制备方法 (Scar-removing ointment and preparation method thereof ) 是由 王丽 李韬韬 于 2021-08-17 设计创作，主要内容包括：一种祛疤膏,包括以下重量份的组分：黄芩苷5—8份,薄荷醇0.1-0.2份,丁香酚1—2份,矿脂4—5份,生长因子KGF-2 3—6份,卡波姆ETD2020 30-40份,三乙醇胺0.1—1份,聚乙二醇-14M 3—4份,苯氧乙醇3—4份,羟苯甲酯3—4份,EDTA二钠3—4份,DMDM乙内酰脲2—3份,薰衣草提纯精油0.1—0.3份,芳香剂1—2份,二醇类防腐剂1—3份,去离子水10—20份。该祛疤膏制备时,先将薄荷醇和黄芩苷加入矿脂和丁香酚混匀,依次加入卡波姆ETD2020、16/18混合醇,三乙醇胺、聚乙二醇—14M、苯氧乙醇、羟苯甲酯、EDTA二钠、DMDM乙內酰脲、生长因子KGF-2、薰衣草提纯精油、芳香剂、离子水、二醇类防腐剂,混匀即得所述祛疤膏。本发明的祛疤膏具有良好的祛疤效果,温和无刺激,祛疤成本低。(The scar-removing ointment comprises the following components in parts by weight: 5-8 parts of baicalin, 0.1-0.2 part of menthol, 1-2 parts of eugenol, 4-5 parts of petrolatum, 23-6 parts of growth factor KGF-6, 202030-40 parts of carbomer ETD, 0.1-1 part of triethanolamine, 3-4 parts of polyethylene glycol-14M, 3-4 parts of phenoxyethanol, 3-4 parts of methylparaben, 3-4 parts of EDTA disodium, 2-3 parts of DMDM hydantoin, 0.1-0.3 part of purified lavender essential oil, 1-2 parts of an aromatic, 1-3 parts of a glycol preservative and 10-20 parts of deionized water. When the scar removing cream is prepared, menthol and baicalin are added into petrolatum and eugenol to be mixed uniformly, then carbomer ETD2020 and 16/18 mixed alcohol, triethanolamine, polyethylene glycol-14M, phenoxyethanol, methylparaben, disodium EDTA, DMDM glycolylurea, growth factor KGF-2, purified lavender essential oil, an aromatic, ionized water and a glycol preservative are added in sequence, and the scar removing cream is prepared after uniform mixing. The scar-removing ointment has good scar-removing effect, is mild and non-irritant, and has low scar-removing cost.)

1. The scar-removing ointment is characterized by comprising the following components in parts by weight: 5-8 parts of baicalin, 0.1-0.2 part of menthol, 1-2 parts of eugenol, 4-5 parts of petrolatum, 23-6 parts of growth factor KGF-6, 202030-40 parts of carbomer ETD, 0.1-1 part of triethanolamine, 3-4 parts of polyethylene glycol-14M, 3-4 parts of phenoxyethanol, 3-4 parts of methylparaben, 3-4 parts of EDTA disodium, 2-3 parts of DMDM hydantoin, 0.1-0.3 part of purified lavender essential oil, 1-2 parts of an aromatic, 1-3 parts of a glycol preservative and 10-20 parts of deionized water.

2. The scar-removing cream according to claim 1, wherein the aromatic is any one or more of jojoba oil, evening primrose oil, mint essential oil, lavender essential oil, lemon essential oil and rose essential oil.

3. The scar-removing cream according to claim 1, wherein the glycol preservative is one or more of butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, and ethylhexylglycerin.

4. The scar-removing cream according to claim 1, wherein the baicalin is prepared by the following steps:

s1, adding Scutellaria baicalensis into 6-8 times of water by weight, decocting for 2 times, each time for 1 hour, combining the two decoctions, adjusting pH to 1-2, keeping the temperature at 80 ℃ for 40 minutes, standing, filtering, washing the filter material with alcohol twice, performing suction filtration, and drying at 50 ℃ to obtain primary baicalin;

s2, putting the primary baicalin obtained in the step S1 into water with the weight 6-8 times of that of the primary baicalin, adjusting the pH to 6-7, adding activated carbon, preserving the temperature for 40 minutes at 80 ℃, adding ethanol, carrying out suction filtration, adjusting the pH of filtrate to 2, preserving the temperature for 40 minutes at 80 ℃, standing for 20 hours, taking upper-layer liquid, washing with ethanol, precipitating and drying to obtain the baicalin.

5. The scar-removing cream according to claim 1, wherein the menthol is prepared by the following method:

s1, drying the mint in the shade, then crushing, and sieving by a 60-mesh sieve to obtain a mint sample;

s2, extracting the mint sample by using supercritical carbon dioxide, wherein the extraction temperature is 60-70 ℃, the extraction pressure is 9.0-9.5Mpa, and the carbon dioxide flow is 20-30 kg/h;

s3, collecting and separating the extract, and filtering to obtain pure peppermint oil.

6. The scar-removing paste according to claim 1, further comprising 30-40 parts of a snake oil compound, wherein the snake oil compound comprises the following components in parts by weight: 30-40 parts of snake oil, 0.1-1 part of trichloro-hydroxydiphenyl ether, 3-4 parts of 16/18 mixed alcohol, 4-6 parts of sodium dodecyl sulfate, 5-10 parts of chlorella growth factor and 4-8 parts of DPS (DPS) active factor.

7. The scar-removing paste according to claim 6, wherein the snake oil compound is prepared by a method comprising the steps of:

s1, adding hot water of 55-85 ℃ into the snake fat tissue serving as the raw material to refine to obtain refined liquid, filtering the refined liquid while the refined liquid is hot to obtain filtrate serving as raw snake oil, and pulping the raw snake oil without impurities to obtain snake oil slurry;

s2, adding hot water of 55-85 degrees into the snake oil slurry, repeatedly washing for 1-5 times, and taking clear liquid to obtain a snake oil stock solution;

s3, adding a decolorizing agent into the snake oil stock solution, refluxing at 50-80 ℃ for 10-60min for decolorization, centrifuging at the rotating speed of 5000-8000 rpm for 5-10min, and taking the clarified solution to obtain a first snake oil solution;

s4, adding 80% of diethyl ether into the first snake oil liquid, and distilling the clear liquid under reduced pressure to obtain a second snake oil liquid;

s5, adding anhydrous ether into the second snake oil liquid, and carrying out reduced pressure distillation to obtain a third snake oil liquid;

s6, adding a mixed solution of anhydrous ether and anhydrous ethanol into the third snake oil, and carrying out reduced pressure distillation to obtain the snake oil used in the formula;

s7, heating the snake oil obtained in the step S6 to 60 ℃, adding trichlorohydroxydiphenyl ether, 16/18 mixed alcohol and sodium dodecyl sulfate, uniformly mixing, adding chlorella growth factor and DPS active factor, and uniformly mixing to obtain the snake oil compound.

8. A method of preparing a scar-removing paste according to any one of claims 1 to 5, comprising the steps of:

s1, adding menthol and baicalin into petrolatum and eugenol, and mixing to obtain baicalin lipid complex;

s2, adding carbomer ETD2020 and 16/18 mixed alcohol, triethanolamine, polyethylene glycol-14M, phenoxyethanol, methylparaben, disodium EDTA and DMDM hydantoin into the baicalin lipid complex, and uniformly mixing to obtain a first baicalin lipid complex gel;

s3, adding growth factor KGF-2 into the first baicalin lipid complex gel, and mixing uniformly to obtain a second baicalin lipid complex gel;

s4, adding lavender purified essential oil, an aromatic, ionized water and a glycol preservative into the second baicalin lipid complex gel, and uniformly mixing to obtain the scar-removing paste.

9. A method of preparing a scar-removing paste according to any one of claims 6 to 7, comprising the steps of:

s1, adding menthol and baicalin into petrolatum and eugenol, and mixing to obtain baicalin lipid complex;

s2, adding carbomer ETD2020 and 16/18 mixed alcohol, triethanolamine, polyethylene glycol-14M, phenoxyethanol, methylparaben, disodium EDTA and DMDM hydantoin into the baicalin lipid complex, and uniformly mixing to obtain a first baicalin lipid complex gel;

s3, adding growth factor KGF-2 into the first baicalin lipid complex gel, and mixing uniformly to obtain a second baicalin lipid complex gel;

s4, adding 40 ° snake oil compound into the second baicalin lipid compound gel, and mixing to obtain a third baicalin snake oil compound;

s5, adding lavender purified essential oil, an aromatic, ionized water and a glycol preservative into the third baicalin-snake oil compound, and uniformly mixing to obtain the scar-removing paste.

Technical Field

The invention relates to the technical field of medicines, in particular to scar removing cream and a preparation method thereof.

Background

The skin is of great importance to the human body, protecting it from external elements. In real life, the skin is damaged due to various reasons to generate scars, for example, burn, scratch, scald, acne mark and the like can generate scars, the nature of the scars is abnormal tissues without normal skin tissue structure and physiological function and normal tissue vitality loss, the scars of the human body not only damage the integrity of the skin of the human body, but also obstruct the physiological function of related tissues or organs, and seriously bring great mental stress to patients, the existing scar removing methods mostly adopt the ways of laser, refrigeration, skin grafting and the like, however, the methods or irritation are too large, so that the side effect is obvious, or the cost is high, and the scar removing cost is too high.

Disclosure of Invention

The scar removing paste adopts a coating mode to remove scars, has a good scar removing effect, is mild and non-irritant, and is low in scar removing cost.

The technical scheme adopted by the invention is as follows:

the scar-removing ointment comprises the following components in parts by weight: 5-8 parts of baicalin, 0.1-0.2 part of menthol, 1-2 parts of eugenol, 4-5 parts of petrolatum, 23-6 parts of growth factor KGF-6, 202030-40 parts of carbomer ETD, 0.1-1 part of triethanolamine, 3-4 parts of polyethylene glycol-14M, 3-4 parts of phenoxyethanol, 3-4 parts of methylparaben, 3-4 parts of EDTA disodium, 2-3 parts of DMDM hydantoin, 0.1-0.3 part of purified lavender essential oil, 1-2 parts of an aromatic, 1-3 parts of a glycol preservative and 10-20 parts of deionized water.

Further, the aromatic is any one or more of jojoba oil, evening primrose oil, peppermint oil, lavender oil, lemon oil, and rose oil.

Further, the glycol preservative is any one or more of butanediol, pentanediol, hexanediol, caprylyl glycol and ethylhexyl glycerin.

Further, the snake oil composition comprises 30-40 parts of snake oil composition, wherein the snake oil composition comprises the following components in parts by weight: 30-40 parts of snake oil, 0.1-1 part of trichloro-hydroxydiphenyl ether, 3-4 parts of 16/18 mixed alcohol, 4-6 parts of sodium dodecyl sulfate, 5-10 parts of chlorella growth factor and 4-8 parts of DPS active factor.

Further, the preparation method of the snake oil compound comprises the following steps:

s1, adding hot water of 55-85 ℃ into the snake fat tissue serving as the raw material to refine to obtain refined liquid, filtering the refined liquid while the refined liquid is hot to obtain filtrate serving as raw snake oil, and pulping the raw snake oil without impurities to obtain snake oil slurry;

s2, adding hot water of 55-85 degrees into the snake oil slurry, repeatedly washing for 1-5 times, and taking clear liquid to obtain a snake oil stock solution;

s3, adding a decolorizing agent into the snake oil stock solution, refluxing at 50-80 ℃ for 10-60min for decolorization, centrifuging at the rotating speed of 5000-;

s4, adding 80% of diethyl ether into the first snake oil liquid, and distilling the clear liquid under reduced pressure to obtain a second snake oil liquid;

s5, adding anhydrous ether into the second snake oil liquid, and carrying out reduced pressure distillation to obtain a third snake oil liquid;

s6, adding a mixed solution of anhydrous ether and anhydrous ethanol into the third snake oil, and carrying out reduced pressure distillation to obtain the snake oil used in the formula;

s7, heating the snake oil obtained in the step S6 to 60 ℃, adding trichlorohydroxydiphenyl ether, 16/18 mixed alcohol and sodium dodecyl sulfate, uniformly mixing, adding chlorella growth factor and DPS active factor, and uniformly mixing to obtain the snake oil compound.

Further, the preparation of the baicalin comprises the following steps:

s1, adding Scutellaria baicalensis into 6-8 times of water by weight, decocting for 2 times, each time for 1 hour, combining the two decoctions, adjusting pH to 1-2, keeping the temperature at 80 ℃ for 40 minutes, standing, filtering, washing the filter material with alcohol twice, performing suction filtration, and drying at 50 ℃ to obtain primary baicalin;

s2, putting the primary baicalin obtained in the step S1 into water with the weight 6-8 times of that of the primary baicalin, adjusting the pH to 6-7, adding activated carbon, preserving the temperature for 40 minutes at 80 ℃, adding ethanol, carrying out suction filtration, adjusting the pH of filtrate to 2, preserving the temperature for 40 minutes at 80 ℃, standing for 20 hours, taking upper-layer liquid, washing with ethanol, precipitating and drying to obtain the baicalin.

Further, the menthol is prepared by the following method:

s1, drying the mint in the shade, then crushing, and sieving by a 60-mesh sieve to obtain a mint sample;

s2, extracting the mint sample by using supercritical carbon dioxide, wherein the extraction temperature is 60-70 ℃, the extraction pressure is 9.0-9.5Mpa, and the carbon dioxide flow is 20-30 kg/h;

s3, collecting and separating the extract, and filtering to obtain pure peppermint oil.

A preparation method of scar removing ointment comprises the following steps:

s1, adding menthol and baicalin into petrolatum and eugenol, and mixing to obtain baicalin lipid complex;

s2, adding carbomer ETD2020 and 16/18 mixed alcohol, triethanolamine, polyethylene glycol-14M, phenoxyethanol, methylparaben, disodium EDTA and DMDM hydantoin into the baicalin lipid complex, and uniformly mixing to obtain a first baicalin lipid complex gel;

s3, adding growth factor KGF-2 into the first baicalin lipid complex gel, and mixing uniformly to obtain a second baicalin lipid complex gel;

s4, adding 40 ° snake oil compound into the second baicalin lipid compound gel, and mixing to obtain a third baicalin snake oil compound;

s5, adding lavender purified essential oil, an aromatic, ionized water and a glycol preservative into the third baicalin-snake oil compound, and uniformly mixing to obtain the scar-removing paste.

The invention has the beneficial effects that:

1. the scutelloside in the scar-removing cream has a wider antibacterial spectrum and has obvious inhibiting effect on pseudomonas aeruginosa, staphylococcus, streptococcus and the like, the scutelloside has an o-diphenol hydroxyl structure and obvious antioxidant activity, inflammatory reaction after skin injury can be reduced by removing hydroxyl free radicals and alkane free radicals, menthol in the scar-removing cream has a better penetration promoting effect on the scutelloside in the process that keratinocyte growth factors stimulate the growth and proliferation of keratinocytes and the process that epithelial cells stimulate the proliferation and the formation of basal layer cells, so that the scutelloside can better exert the anti-inflammatory, antibacterial and antioxidant effects, the anti-infection capacity of scar wound surfaces is effectively improved, the keratinocyte growth factors stimulate the epithelial cells through specific receptors of the epithelial cells under the action of the scutelloside, and simultaneously, the coordination signal feedback of the epithelial cells to subcutaneous interstitial tissues can be started, promoting the formation of new tissues, accelerating the healing of the wound surface, improving the healing appearance of the wound surface and improving the scar removing effect.

2. After the baicalin and the menthol are respectively treated by adopting the special extraction process, the purity of the baicalin and the menthol can be improved, the effect of the baicalin and the menthol applied to the scar removing cream is improved, the capacity of the scar removing cream for resisting the proliferation of metamorphic cells is enhanced, the anti-allergic reaction and the anti-allergic property of the scar removing cream are obviously enhanced, and the scar removing effect is improved.

3. The chlorella in the snake oil compound adopted by the invention not only has a plurality of abundant proteins and reasonable amino acid composition, but also has abundant active bioactive substances, S-nuclear dai peptide in chlorella colloidal protein and 5-hydroxytryptamine in snake oil are catalyzed by a triaminohydroxylase to firstly generate 5-hydroxytryptamine, and then the 5-hydroxytryptamine is catalyzed by a 5-hydroxytryptamine decarboxylase to generate the 5-hydroxytryptamine, wherein the 5-hydroxytryptamine is a strong blood vessel shrinking agent and a smooth muscle contraction stimulant, has double effects of relieving and inhibiting local muscle activity, recovers elastic tissues of each layer of skin, and has the function of blocking conduction between nerve muscles, avoids over contraction of each layer of skin, and effectively prevents scar contracture. The chlorella collagen extract contains protein, nucleic acid, polysaccharides, various vitamins, amino acids and the like, has the effect of inhibiting the proliferation of viruses and cancer cells, and has the following effects by adding DPS active factors into the snake oil compound:

(1) toxin removal and rapid repair of body injury;

(2) the weak acidity of the skin is improved to be weak alkalinity;

(3) activating cell tissue activity, and enhancing the capability of the cell to resist virus and bacteria invading the outside;

(4) increasing hematopoietic function;

(5) promoting the synthesis of white blood cells;

(6) resisting variant cell proliferation, and resisting cancer;

(7) anti-allergic reaction, and has antiallergic effect;

(8) inhibiting excessive hyperplasia of scar pigment and permeating into skin, recovering skin pigment, and reducing leukoplakia and melanin.

4. The scar-removing paste can remove scars by coating, is mild and non-irritant, and has low scar-removing cost.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following examples, wherein the raw materials and instruments used in the following examples are commercially available, and the data obtained in the examples of the present invention are average values of three or more repeated experiments unless otherwise specified. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Example 1

The scar-removing ointment comprises the following components in parts by weight: 5 parts of baicalin, 0.1 part of menthol, 1 part of eugenol, 4 parts of petrolatum, 23 parts of growth factor KGF-23, 202030 parts of carbomer ETD, 0.1 part of triethanolamine, 3 parts of polyethylene glycol-14M, 3 parts of phenoxyethanol, 3 parts of methylparaben, 3 parts of EDTA disodium, 2 parts of DMDM hydantoin, 0.1 part of lavender purified essential oil, 1 part of aromatic, 1 part of glycol preservative and 10 parts of deionized water.

Example 2

The scar-removing ointment comprises the following components in parts by weight: 6.5 parts of baicalin, 0.1 part of menthol, 1.5 parts of eugenol, 4.5 parts of petrolatum, 24.5 parts of growth factor KGF-24, 202035 parts of carbomer ETD, 0.5 part of triethanolamine, 3.5 parts of polyethylene glycol-14M, 3.5 parts of phenoxyethanol, 3.5 parts of methylparaben, 3.5 parts of EDTA disodium, 2.5 parts of DMDM hydantoin, 0.2 part of lavender purified essential oil, 1.5 parts of aromatic, 2 parts of glycol preservative and 15 parts of deionized water.

Example 3

The scar-removing ointment comprises the following components in parts by weight: 8 parts of baicalin, 0.2 part of menthol, 2 parts of eugenol, 5 parts of petrolatum, 26 parts of growth factor KGF-26, 202040 parts of carbomer ETD, 1 part of triethanolamine, 4 parts of polyethylene glycol-14M, 4 parts of phenoxyethanol, 4 parts of methylparaben, 4 parts of EDTA disodium, 3 parts of DMDM hydantoin, 0.3 part of lavender purified essential oil, 2 parts of aromatic, 3 parts of glycol preservative and 20 parts of deionized water.

Example 4

The scar-removing ointment comprises the following components in parts by weight: 6.5 parts of baicalin, 0.1 part of menthol, 1.5 parts of eugenol, 4.5 parts of petrolatum, 24.5 parts of growth factor KGF-24, 202035 parts of carbomer ETD, 35 parts of snake oil compound, 0.5 part of triethanolamine, 3.5 parts of polyethylene glycol-14M, 3.5 parts of phenoxyethanol, 3.5 parts of methylparaben, 3.5 parts of EDTA disodium, 2.5 parts of DMDM hydantoin, 0.2 part of lavender purified essential oil, 1.5 parts of aromatic, 2 parts of glycol preservative and 15 parts of deionized water.

The snake oil compound comprises the following components in parts by weight: 30 parts of snake oil, 0.1 part of trichloro-hydroxy diphenyl ether, 3 parts of 16/18 mixed alcohol, 4 parts of sodium dodecyl sulfate, 5 parts of chlorella growth factor and 4 parts of DPS active factor.

According to the formula, the snake oil compound is prepared by the following method:

s1, adding hot water of 55 ℃ into the snake fat tissue serving as the raw material to refine to obtain refined liquid, filtering the refined liquid while the refined liquid is hot to obtain filtrate serving as raw snake oil, and pulping the raw snake oil with impurities removed to obtain snake oil slurry;

s2, adding hot water of 55 degrees into the snake oil slurry, repeatedly washing for 1 time, and taking clear liquid to obtain a snake oil stock solution;

s3, adding a decolorizing agent into the snake oil stock solution, refluxing for 10min at 50 ℃, decolorizing, centrifuging for 5min at the rotating speed of 5000 r/min, and taking the clarified solution to obtain a first snake oil solution;

s4, adding 80% of diethyl ether into the first snake oil liquid, and distilling the clear liquid under reduced pressure to obtain a second snake oil liquid;

s5, adding anhydrous ether into the second snake oil liquid, and carrying out reduced pressure distillation to obtain a third snake oil liquid;

s6, adding a mixed solution of anhydrous ether and anhydrous ethanol into the third snake oil, and carrying out reduced pressure distillation to obtain the snake oil used in the formula;

s7, heating the snake oil obtained in the step S6 to 60 ℃, adding trichlorohydroxydiphenyl ether, 16/18 mixed alcohol and sodium dodecyl sulfate, uniformly mixing, adding chlorella growth factor and DPS active factor, and uniformly mixing to obtain the snake oil compound.

Example 5

The scar-removing ointment comprises the following components in parts by weight: 6.5 parts of baicalin, 0.1 part of menthol, 1.5 parts of eugenol, 4.5 parts of petrolatum, 24.5 parts of growth factor KGF-24, 202035 parts of carbomer ETD, 35 parts of snake oil compound, 0.5 part of triethanolamine, 3.5 parts of polyethylene glycol-14M, 3.5 parts of phenoxyethanol, 3.5 parts of methylparaben, 3.5 parts of EDTA disodium, 2.5 parts of DMDM hydantoin, 0.2 part of lavender purified essential oil, 1.5 parts of aromatic, 2 parts of glycol preservative and 15 parts of deionized water.

The snake oil compound comprises the following components in parts by weight: 35 parts of snake oil, 0.5 part of trichloro-hydroxy diphenyl ether, 3.5 parts of 16/18 mixed alcohol, 5 parts of sodium dodecyl sulfate, 8 parts of chlorella growth factor and 6 parts of DPS active factor.

According to the formula, the snake oil compound is prepared by the following method:

s1, adding 70 ℃ hot water into the snake fat tissue serving as the raw material to refine to obtain refined liquid, filtering the refined liquid while the refined liquid is hot to obtain a filtrate serving as raw snake oil, and pulping the raw snake oil with impurities removed to obtain snake oil slurry;

s2, adding 70-degree hot water into the snake oil slurry, repeatedly washing for 3 times, and taking a clear liquid to obtain a snake oil stock solution;

s3, adding a decolorizing agent into the snake oil stock solution, refluxing for 40min at 65 ℃, decolorizing, centrifuging for 7min at the rotating speed of 6500 r/min, and taking a clarified solution to obtain a first snake oil solution;

s4, adding 80% of diethyl ether into the first snake oil liquid, and distilling the clear liquid under reduced pressure to obtain a second snake oil liquid;

s5, adding anhydrous ether into the second snake oil liquid, and carrying out reduced pressure distillation to obtain a third snake oil liquid;

s6, adding a mixed solution of anhydrous ether and anhydrous ethanol into the third snake oil, and carrying out reduced pressure distillation to obtain the snake oil used in the formula;

s7, heating the snake oil obtained in the step S6 to 60 ℃, adding trichlorohydroxydiphenyl ether, 16/18 mixed alcohol and sodium dodecyl sulfate, uniformly mixing, adding chlorella growth factor and DPS active factor, and uniformly mixing to obtain the snake oil compound.

Example 6

The scar-removing ointment comprises the following components in parts by weight: 6.5 parts of baicalin, 0.1 part of menthol, 1.5 parts of eugenol, 4.5 parts of petrolatum, 24.5 parts of growth factor KGF-24, 202035 parts of carbomer ETD, 35 parts of snake oil compound, 0.5 part of triethanolamine, 3.5 parts of polyethylene glycol-14M, 3.5 parts of phenoxyethanol, 3.5 parts of methylparaben, 3.5 parts of EDTA disodium, 2.5 parts of DMDM hydantoin, 0.2 part of lavender purified essential oil, 1.5 parts of aromatic, 2 parts of glycol preservative and 15 parts of deionized water.

The snake oil compound comprises the following components in parts by weight: 40 parts of snake oil, 1 part of trichloro-hydroxy diphenyl ether, 4 parts of 16/18 mixed alcohol, 6 parts of sodium dodecyl sulfate, 10 parts of chlorella growth factor and 8 parts of DPS active factor.

According to the formula, the snake oil compound is prepared by the following method:

s1, adding hot water of 85 ℃ into the snake fat tissue serving as the raw material to refine to obtain refined liquid, filtering the refined liquid while the refined liquid is hot to obtain filtrate serving as raw snake oil, and pulping the raw snake oil without impurities to obtain snake oil slurry.

S2, adding 85-degree hot water into the snake oil slurry, repeatedly washing for 5 times, and taking a clear liquid to obtain a snake oil stock solution;

s3, adding a decolorizing agent into the snake oil stock solution, refluxing at 80 ℃ for 60min for decolorization, centrifuging at 8000 rpm for 10min, and taking the clear solution to obtain a first snake oil solution.

S4, adding 80% of diethyl ether into the first snake oil liquid, and distilling the clear liquid under reduced pressure to obtain a second snake oil liquid.

And S5, adding anhydrous ether into the second snake oil liquid, and carrying out reduced pressure distillation to obtain a third snake oil liquid.

S6, adding a mixed solution of anhydrous ether and anhydrous ethanol into the third snake oil, and distilling under reduced pressure to obtain the snake oil used in the formula.

S7, heating the snake oil obtained in the step S6 to 60 ℃, adding trichlorohydroxydiphenyl ether, 16/18 mixed alcohol and sodium dodecyl sulfate, uniformly mixing, adding chlorella growth factor and DPS active factor, and uniformly mixing to obtain the snake oil compound.

Example 7

The scar-removing ointment comprises the following components in parts by weight: 8 parts of baicalin, 0.2 part of menthol, 2 parts of eugenol, 5 parts of petrolatum, 26 parts of growth factor KGF-26, 202040 parts of carbomer ETD, 40 parts of snake oil compound, 1 part of triethanolamine, 4 parts of polyethylene glycol-14M, 4 parts of phenoxyethanol, 4 parts of methylparaben, 4 parts of EDTA disodium, 3 parts of DMDM hydantoin, 0.3 part of lavender purified essential oil, 2 parts of an aromatic, 3 parts of a glycol preservative and 20 parts of deionized water.

Wherein, the baicalin is prepared by the following method, which comprises the following steps:

s1, adding Scutellaria baicalensis into 6-8 times of water by weight, decocting for 2 times, each time for 1 hour, combining the two decoctions, adjusting the pH to 1-2, keeping the temperature at 80 ℃ for 40 minutes, standing, filtering, washing a filter material with ethanol or propanol twice, performing suction filtration, and drying the filter material at 50 ℃ to obtain primary baicalin;

s2, putting the primary baicalin obtained in the step S1 into water with the weight of 6-8 times, adjusting the pH value to 6-7, adding activated carbon, preserving the temperature at 80 ℃ for 40 minutes, adding ethanol, carrying out suction filtration, adjusting the pH value of filtrate to 2, preserving the temperature at 80 ℃ for 40 minutes, standing for 20 hours, taking upper-layer liquid, washing with ethanol, precipitating and drying to obtain the baicalin.

Example 8

The scar-removing ointment comprises the following components in parts by weight: 8 parts of baicalin, 0.2 part of menthol, 2 parts of eugenol, 5 parts of petrolatum, 26 parts of growth factor KGF-26, 202040 parts of carbomer ETD, 40 parts of snake oil compound, 1 part of triethanolamine, 4 parts of polyethylene glycol-14M, 4 parts of phenoxyethanol, 4 parts of methylparaben, 4 parts of EDTA disodium, 3 parts of DMDM hydantoin, 0.3 part of lavender purified essential oil, 2 parts of an aromatic, 3 parts of a glycol preservative and 20 parts of deionized water.

The menthol is prepared by supercritical carbon dioxide extraction, and specifically comprises the following steps:

s1, drying the mint in the shade, then crushing the mint, and screening the mint through a 60-mesh sieve to obtain a mint sample.

S2, extracting the mint sample by using carbon dioxide, putting the mint sample into a supercritical carbon dioxide extraction kettle, adjusting the extraction temperature of the supercritical extraction kettle to be 60-70 ℃, the extraction pressure to be 9.0-9.5Mpa, and adjusting the flow of the carbon dioxide to be 20-30 kg/h.

S3, collecting the extract from the supercritical carbon dioxide extraction kettle, separating the extract through a separating funnel, removing residual water by using anhydrous sodium sulfate, and filtering to obtain the menthol.

The invention also provides a preparation method of the scar-removing paste, which comprises the following steps:

s1, adding petrolatum and eugenol into menthol and baicalin, and mixing to obtain baicalin lipid complex.

S2, adding carbomer ETD2020 and 16/18 mixed alcohol, triethanolamine, polyethylene glycol-14M, phenoxyethanol, methylparaben, disodium EDTA and DMDM hydantoin into the baicalin lipid complex, and uniformly mixing to obtain the first baicalin lipid complex gel.

S3, adding growth factor KGF-2 into the first baicalin lipid complex gel, and mixing to obtain a second baicalin lipid complex gel.

S4, adding the snake oil compound heated to 40 deg.C into the second baicalin lipid compound gel, and mixing to obtain the third baicalin snake oil compound.

S5, adding lavender purified essential oil, aromatic, ionic water and glycol preservative into the third baicalin-snake oil compound, and mixing uniformly to obtain the scar-removing ointment.

The scar-removing cream of each example can be prepared by weighing the components according to the mixture ratio of the components in the examples 1 to 8 and adopting the preparation method.

Bacteriostasis test

Test subjects: the scar-removing cream obtained in the embodiments 1 to 8 of the invention.

The test method comprises the following steps: the antibacterial effect of the scar-removing paste on the strains to be detected (escherichia coli, pseudomonas aeruginosa, staphylococcus and streptococcus) is measured by adopting an agar diffusion paper sheet method and a colony counting method. Preparing 108/mL bacterial suspension of a strain to be detected, and uniformly coating the bacterial suspension on the surface of a solidified nutrient agar culture medium plate, wherein each plate is 100 mu L; uniformly coating each scar-removing paste on a filter paper sheet with the diameter of 0.3cm and subjected to sterilization and drying under an aseptic condition, adhering prepared scar-removing paste filter paper sheets (taking the sterilization filter paper sheets as a blank control) on plates coated with test strains, culturing for 24 hours at a constant temperature of 37 ℃, taking a contact surface culture as gradient dilution, taking 1mL of the diluted bacterial suspension in the plates, pouring agar into the plates, uniformly mixing, after solidification, inversely placing at the constant temperature of 37 ℃ for culturing for 24 hours, calculating the total number of bacterial colonies, referring to GB 47892-.

Bacteriostatic ratio (%) - (total number of blank colonies-total number of gel colonies)/total number of blank colonies × 100

TABLE 1 bacteriostatic effect of scar-removing cream in each example

Group of	Escherichia coli	Pseudomonas aeruginosa	Staphylococcus aureus	Streptococcus sp
					Example 1	92.6％	90.4％	92.1％	91.0％
Example 2	93.1％	92.2％	92.7％	92.3％
					Example 3	91.7％	91.1％	91.3％	90.7％
Example 4	98.9％	97.2％	97.6％	96.1％
					Example 5	98.9％	97.2％	97.6％	96.1％
Example 6	95.3％	93.7％	94.2％	93.9％
					Example 7	95.3％	93.7％	94.2％	93.9％
Example 8	94.8％	93.1％	94.3％	94.6％

Clinical trial

1. Data and method

1.1 general data: 242 patients with bruises and burns in 2018 to 2021 in 6 months of Luoyang city, Henan province, 138 men, 104 women, 6 to 70 years of age, 35 +/-2 years of average age, 36 patients with acne marks left on facial acne residues in 2019 to 2021 in 6 months, 31 men, 5 women and 19 +/-1 years of average age.

1.2 methods of grouping and administering drugs

Grouping: the 242 patients with the scratches and the burns were randomly divided into 8 groups, wherein 30 patients were randomly assigned to each of 1 to 7 groups, 32 patients were assigned to group 8, and 1 to 8 groups of patients were individually treated with the scar-removing cream prepared in examples 1 to 8, and 3 groups of 36 patients with acne marks left on the face were randomly assigned as 9 to 11 groups, wherein group 9 was treated with the scar-removing cream prepared in example 1, group 10 was treated with the scar-removing cream prepared in example 4, and group 11 was treated with the scar-removing cream prepared in example 5.

The administration method comprises the following steps: under aseptic operation, the wound surfaces of bruises and burns are disinfected conventionally, the wound surfaces are cleaned simply by using low-concentration iodophor and normal saline, attachments and fallen wrinkled skin are removed, the water is discharged by puncture of a high-tension vesicle, the residual epithelium is kept as much as possible, the fallen or necrotic epidermis is cut off, the infected wound surfaces are thoroughly debrided, each corresponding scar removing ointment is coated outside the wound surfaces, the medicine is applied for 1-3 times every day, and the corresponding scar removing ointment is coated after the wound surfaces of acne scars on the face are disinfected conventionally. The wound surface is fully exposed after the medicine is taken, the wound surface cannot be covered, water is prevented from entering, warm keeping is realized in winter, and the dosage of each group is determined according to the areas of bruise, burn and scald and the area of acne marks so as to cover the wound surface completely.

1.3 healing efficacy criteria: the swelling disappears, the pain disappears, the wound surface has no infection, the scab heals, the epidermis grows, and the wound surface is basically repaired or completely repaired. Defining the time required by the complete epithelialization of the wound surface as the healing time, and recording the healing time of each patient; the scar condition of the wound surface of the burn and scald patients is recorded, the number of the patients with scars is recorded, the scar rate is calculated, the scar rate is equal to the number of the patients with scars/the total number of cases multiplied by 100%, and the clinical test results are shown in table 2.

TABLE 2 clinical trial results for each test group

As can be seen from table 2, the scar removing cream obtained in examples 1 to 8 of the present invention has excellent effects when applied to the treatment of patients with bruises and burns, and particularly, the scar removing cream containing the snake oil compound has a significantly reduced scar rate, and the experiment on the scar removing effect of the patients with acne and acne marks left on the face shows that the scar removing cream without the snake oil compound has a general effect on removing acne marks, and the scar removing effect of the scar removing cream containing the snake oil compound on the face of the patient is significantly better than that of the scar removing cream with the snake oil compound.

It should be noted that the above embodiments are only for illustrating the present invention, but the present invention is not limited to the above embodiments, and any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention fall within the protection scope of the present invention.