阅读说明：本技术 一种利用毛细管管壁轴向全反射的检测池 (A kind of detection cell being axially totally reflected using capillary wall ) 是由 杨三东 唐涛 封娇 李彤 于 2019-08-30 设计创作，主要内容包括：本发明公开了一种利用毛细管管壁轴向全反射的检测池,涉及一种利用色谱法将材料分离成各个组分,来测试或分析材料的液相色谱系统中的光检测器。包括光学吸收型检测器,光学吸收型检测器用于液相色谱系统检测样品；光学吸收型检测器包括流路入口,检测池和流路出口；流路入口和流路出口固定连接于检测池；检测池为毛细管,毛细管为透明材料制成,毛细管折射率大于样品的折射率；毛细管外管壁外侧四周设置为空气或真空,样品的折射率大于所述空气或真空的折射率。本发明利用了毛细管外壁全反射现象在增大光程减小内径的情况下减少了光能损失,提高了检测的灵敏度；且入射光的临界角角度更大。(The invention discloses a kind of detection cell being axially totally reflected using capillary wall, be related to it is a kind of material is separated into each component using chromatography, come test or the liquid chromatographic system of analysis of material in photodetector.Including optical absorption type detector, optical absorption type detector is used for liquid chromatographic system test sample；Optical absorption type detector includes path inlet, detection cell and flowing path outlet；Path inlet and flowing path outlet are fixedly connected on detection cell；Detection cell is capillary, and capillary is made of transparent material, and capillary refractive index is greater than the refractive index of sample；Surrounding is set as air or vacuum on the outside of extracapillary tube wall, and the refractive index of sample is greater than the refractive index of the air or vacuum.Present invention utilizes capillary outer wall total reflection phenomenons to reduce optical energy loss in the case where increasing light path and reducing internal diameter, improves the sensitivity of detection；And the critical angle angle of incident light is bigger.)

1. a kind of detection cell being axially totally reflected using capillary wall, including optical absorption type detector, the optical absorption Type detector is used for liquid chromatographic system test sample, and the optical absorption type detector includes path inlet, detection cell and stream Way outlet, the path inlet and flowing path outlet are fixedly connected on detection cell, it is characterised in that: and the detection cell is capillary, The capillary is made of transparent material, and the capillary refractive index is greater than the refractive index of the sample, the capillary outer tube Surrounding is set as air or vacuum on the outside of wall, and the refractive index of the sample is greater than the refractive index of the air or vacuum.

2. a kind of detection cell being axially totally reflected using capillary wall according to claim 1, it is characterised in that: also set It is equipped with casing, described sleeve pipe is sheathed on capillary.

3. a kind of detection cell being axially totally reflected using capillary wall according to claim 2, it is characterised in that: described Air or vacuum are set as between casing and capillary.

4. a kind of detection cell being axially totally reflected using capillary wall according to claim 1, it is characterised in that: described Capillary pipe length is 10mm-200mm.

5. a kind of detection cell being axially totally reflected using capillary wall according to claim 1, it is characterised in that: described Capillary wall is with a thickness of 10 μm -360 μm.

6. a kind of detection cell being axially totally reflected using capillary wall according to claim 1, it is characterised in that: described Capillary inner diameter is 5 μm -530 μm.

7. a kind of detection cell being axially totally reflected using capillary wall according to claim 1, it is characterised in that: described Capillary is one of quartz glass, borosilicate glass, quartz glass doped graphite or borosilicate glass doped graphite Material is made.

Technical field

Material is separated into each component using chromatography the present invention relates to a kind of, to test or the liquid phase color of analysis of material Photodetector in spectra system, especially a kind of detection cell being axially totally reflected using capillary wall.

Background technique

Optical absorption type detector based on langbobier law is most common one kind detector in liquid chromatographic system. Detection cell is core component therein, and when sample flows through detection cell, light passes through the sample in detection cell, and is inhaled by sample part It receives.The absorbance information of sample can be obtained in the intensity of record incident light and emergent light.Theoretically, the light path of detection cell is longer, The absorbance of sample is bigger, and detector sensitivity is higher.But in the case that in pond hole, internal diameter is constant, light path is longer, and pond body product is got over Greatly, diffusion of the sample in detection cell is more obvious, and can reduce the sensitivity of detection.In the case where increasing light path, pass through reduction The internal diameter in pond hole is a solution to control pond body to accumulate size, but the reduction of pond hole internal diameter can cause the drop of luminous flux again It is low, so that the influence of noise is become more significant, can equally cause the reduction of sensitivity.The detection of normal optical absorption-type detector Pond is typically designed as light path 8-10mm, Chi Kong 1mm or so, 10 μ L of pond body product or so.When being for isolated liquid chromatographic system When micro column liquid chromatography or ultra performance liquid chromatography, it is necessary to the detection cell with more highly sensitive, lower pond body product.

To solve this problem, laboratory mostly uses the mode detected on column at present, that is, removes the partial coating of capillary, And it regard this part as detection window.This mode can significantly reduce detection cell pond body product, but only due to the light path of detection Suitable with capillary inner diameter, detection sensitivity is extremely low.Part commercialization instrument reduces pond bore dia in the way of precision machined, It is optimized on optical focusing system simultaneously, it is intended to improve luminous flux, but this mode is to processing and the required precision focused It is higher, it is not easy to realize.In addition there are a kind of materials using refractive index less than mobile phase as detection pool wall, to realize detection cell The pool wall material of the design scheme being totally reflected on inner wall, the program is more expensive, and pool wall Refractive Index of Material and mobile phase reflect Rate is very close, only slightly less than mobile phase refractive index, therefore ranges of incidence angles when being totally reflected is smaller, to light focusing energy More stringent requirements are proposed for power.

" a kind of liquid chromatogram ultraviolet and visible light detector is all-trans the Chinese utility model of Publication No. CN205120679U Penetrate overlength light path detection cell " provide a kind of liquid chromatogram ultraviolet and visible light detector total reflection overlength light path detection cell, packet Include detection cell tube wall, total reflection liner, inlet tube, outlet tube, entrance clamping pad, outlet clamping pad, entrance pool window, outlet bath Window, entrance pool window gasket and outlet bath window gasket.Attachment total reflection liner, makes light between pool wall on the inside of detection pool wall Basic no consumption reflection, really widening reaches to make it possible to increase detection cell optical path length and improves detector spirit less The purpose of sensitivity.A kind of macromolecule for being called TEFLONAF that the total reflection liner that this utility model uses is developed for E.I.Du Pont Company Material, refractive index are 1.29-1.31 or so, are slightly less than the common aqueous solvent in liquid chromatogram field, methanol and acetonitrile, when above-mentioned When three kinds of solvents cross detection cell with sample flow, on the inside of detection pool wall total reflection phenomenon can occur for light, improve detector spirit Sensitivity.But TEFLONAF high molecular material is unique selection, and the refractive index of other materials is difficult to lower than common solvent, and its valence Lattice are very expensive, are not easy to obtain；It, can and since TEFLONAF high molecular material refractive index and solvent refractive index are little The critical angle range being totally reflected is smaller.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of optical length, pond is small in size, high sensitivity, can be all-trans The detection cell that the critical angle range penetrated is big, material is easy to get and structure simply utilizes capillary wall to be axially totally reflected.

To solve the above problems, the technical solution of the present invention is as follows:

A kind of detection cell being axially totally reflected using capillary wall, including optical absorption type detector, the optics are inhaled Receipts type detector is used for liquid chromatographic system test sample, and the optical absorption type detector includes path inlet, detection cell and Flowing path outlet, the path inlet and flowing path outlet are fixedly connected on detection cell, and the detection cell is capillary, the capillary It is made of transparent material, the capillary refractive index is greater than the refractive index of the sample, surrounding on the outside of the extracapillary tube wall It is set as air or vacuum, the refractive index of the sample is greater than the refractive index of the air or vacuum.

Further, it is additionally provided with casing, described sleeve pipe is sheathed on capillary.

Further, air or vacuum are set as between described sleeve pipe and capillary.

Further, the capillary pipe length is 10mm-200mm.

Further, the capillary wall is with a thickness of 10 μm -360 μm.

Further, the capillary inner diameter is 5 μm -530 μm.

Further, the capillary is quartz glass, borosilicate glass, quartz glass doped graphite or borosilicate One of glass doped graphite material is made.

Compared with prior art, the invention has the following beneficial effects:

1. present invention utilizes capillary outer wall total reflection phenomenons to reduce in the case where increasing light path and reducing internal diameter Optical energy loss improves the sensitivity of detection；

2. the critical angle angle that the present invention has bigger incident light；

3. the present invention is using common quartz capillary as absorbance region, material is easy to get, and the coupling of light and flow path is held Easily realize, it is easy to process；

4. the present invention can replace the capillary of different length as needed, the use for having adapted to different detection sensitivities is needed It asks.

Detailed description of the invention

Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.

Fig. 1 is capillary cross-sectional view schematic diagram of the present invention；

Wherein, 1 is tube body；2 be sample；3 be light of not being rebuffed；4 be total reflection light；5 be outer wall；6 be injection light.

Specific embodiment

In order to be easy to understand to technical means, creative features, achievable purpose and effectiveness of the invention, below with reference to tool Body diagram, the present invention is further explained.