阅读说明：本技术 一种卷烟主流烟气中香味成分的检测方法 (Method for detecting aroma components in cigarette mainstream smoke ) 是由 王晓瑜 郭琼 刘惠民 秦亚琼 潘立宁 谢复炜 赵晓东 贾云祯 陈满堂 刘瑞红 于 2021-08-20 设计创作，主要内容包括：本发明涉及一种卷烟主流烟气中香味成分的检测方法,属于烟气成分分析领域。一种卷烟主流烟气中香味成分的检测方法,包括以下步骤：(1)采用滤片捕集卷烟主流烟气中的粒相物,然后采用萃取处理液对捕集粒相物后的滤片进行萃取处理,得到萃取液；(2)在所述萃取液中加入分析保护剂,得到待测液；(3)将所述待测液进行气相色谱-串联质谱分析。本发明的卷烟主流烟气中香味成分的检测方法具有快速、准确、灵敏、成本低、易操作、通量高、精密度高和重复性好的优点,可同时对多种半挥发性香味成分进行测定,可满足对大批量样品进行快速分析检测的需要。(The invention relates to a method for detecting flavor components in main stream smoke of cigarettes, and belongs to the field of smoke component analysis. A method for detecting flavor components in cigarette mainstream smoke comprises the following steps: (1) adopting a filter disc to capture particulate matters in main stream smoke of the cigarette, and then adopting extraction treatment liquid to perform extraction treatment on the filter disc after the particulate matters are captured, so as to obtain extraction liquid; (2) adding an analysis protective agent into the extract to obtain a solution to be detected; (3) and carrying out gas chromatography-tandem mass spectrometry on the liquid to be detected. The method for detecting the aroma components in the mainstream smoke of the cigarettes has the advantages of rapidness, accuracy, sensitivity, low cost, easiness in operation, high flux, high precision and good repeatability, can be used for simultaneously detecting various semi-volatile aroma components, and can meet the requirement of rapidly analyzing and detecting mass samples.)

1. A method for detecting flavor components in cigarette mainstream smoke is characterized by comprising the following steps:

(1) adopting a filter disc to capture particulate matters in main stream smoke of the cigarette, and then adopting extraction treatment liquid to perform extraction treatment on the filter disc after the particulate matters are captured, so as to obtain extraction liquid; the extraction treatment liquid comprises an extraction solvent and an internal standard substance; the internal standard substance is selected from one or any combination of acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4;

(2) adding an analysis protective agent into the extract to obtain a solution to be detected; the analysis protective agent is dihydric alcohol;

(3) carrying out gas chromatography-tandem mass spectrometry on the liquid to be detected; the chromatographic column of the gas chromatography in the gas chromatography-tandem mass spectrometry is an elastic quartz capillary chromatographic column, and the stationary phase is phenyl-methyl polysiloxane with the mass fraction of 50%.

2. The method of claim 1, wherein said internal standard comprises acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4.

3. The method for detecting the flavor components in the mainstream smoke of the cigarette as claimed in claim 2, wherein the mass ratio of the acetophenone-d 8, the styrene-d 8, the benzophenone-d 10 and the diisobutyl phthalate-d 4 is 1-6: 1-6.

4. The method of claim 1, wherein the diol is selected from the group consisting of octanediol, nonanediol, and tetradecanediol, or any combination thereof.

5. The method of claim 4, wherein said glycol is comprised of octanediol, nonanediol, and tetradecanediol.

6. The method for detecting the flavor components in the mainstream smoke of the cigarette as claimed in claim 5, wherein the mass ratio of the octanediol, the nonanediol and the tetradecanediol is 1-6: 1-6.

7. The method for detecting flavor components in mainstream smoke of cigarettes according to claim 1, wherein the gas chromatography-tandem mass spectrometry comprises the following conditions: the temperature of a sample inlet is 280 ℃; and (5) temperature programming.

8. The method for detecting flavor components in mainstream smoke of cigarettes according to claim 1, wherein the conditions of mass spectrometry in the gas chromatography-tandem mass spectrometry comprise: the ionization mode is electron bombardment ionization; ionization energy is 70 eV; the ion source temperature is 280 ℃; the temperature of the four-level bar is 150 ℃; the temperature of the transmission line is 280 ℃; the scanning mode is dynamic multi-reaction monitoring.

9. The method of claim 1, wherein the filter is a Cambridge filter.

10. The method for detecting flavor components in mainstream smoke of cigarettes according to any one of claims 1 to 9, wherein the flavor components are selected from one or any combination of the following compounds:

Technical Field

The invention relates to a method for detecting flavor components in main stream smoke of cigarettes, and belongs to the field of smoke component analysis.

Background

The aroma components in the smoke comprise various semi-volatile components such as aldehyde, ketone, alcohol, phenol, acid, ether, ester, lactone, alkene, pyridine, pyrrole, pyrazine and the like, and the content level and the mutual proportion of the components determine the sensory style and the quality characteristics of the cigarette.

Separation and detection have been difficult due to the extremely complex composition of mainstream smoke. The development of a full two-dimensional gas chromatography-mass spectrometry (GC × GC/MS) technology in the last 90 s of the century greatly improves the chromatographic peak capacity of GC, but the problems of false positive results, overload and difficult alignment of chromatographic peaks still commonly exist in the qualitative analysis of GC × GC/MS, so that the GC × GC/MS is mainly used for the qualitative analysis of smoke components and is difficult to be used for daily quantitative detection. The method is limited by the problems of sample matrix interference, insufficient GC/MS sensitivity and the like, the accuracy of quantitative analysis of cigarette mainstream smoke components at home and abroad is poor at present, the types and the quantity of flavor components in smoke capable of being quantitatively analyzed are limited, semi-quantitative analysis is generally carried out by adopting the ratio of the peak area of each smoke component to the peak area of an internal standard substance, and absolute quantitative analysis of each smoke component is not carried out. Therefore, a method for accurately and quantitatively detecting the aroma components in the mainstream smoke of the cigarettes is urgently needed to be established.

Disclosure of Invention

The invention aims to provide a method for detecting aroma components in cigarette mainstream smoke, which solves the problem of poor accuracy of quantitative analysis of cigarette mainstream smoke components at present.

In order to achieve the aim, the technical scheme of the method for detecting the flavor components in the mainstream smoke of the cigarettes is as follows:

a method for detecting flavor components in cigarette mainstream smoke comprises the following steps:

(1) adopting a filter disc to capture particulate matters in main stream smoke of the cigarette, and then adopting extraction treatment liquid to perform extraction treatment on the filter disc after the particulate matters are captured, so as to obtain extraction liquid; the extraction treatment liquid comprises an extraction solvent and an internal standard substance; the internal standard substance is selected from one or any combination of acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4;

(2) adding an analysis protective agent into the extract to obtain a solution to be detected; the analysis protective agent is dihydric alcohol;

(3) carrying out gas chromatography-tandem mass spectrometry on the liquid to be detected; the chromatographic column of the gas chromatography in the gas chromatography-tandem mass spectrometry is an elastic quartz capillary chromatographic column, and the stationary phase is phenyl-methyl polysiloxane with the mass fraction of 50%.

In gas chromatography analysis, matrix effects are prevalent and often manifest as matrix-induced enhancement. Due to the existence of the active sites, the response value of the substance to be detected with the same content in the actual sample is higher than that in the pure solvent, so that the recovery rate of the matrix adding standard is higher. The invention takes the dihydric alcohol as the analyte protective agent, can effectively cover the active points of the sample inlet and the chromatographic system, corrects the quantitative error introduced by the matrix effect and ensures that the quantitative analysis result is more accurate.

The method for detecting the aroma components in the mainstream smoke of the cigarettes has the advantages of rapidness, accuracy, sensitivity, low cost, easiness in operation, high flux, high precision and good repeatability, can be used for simultaneously detecting 292 semi-volatile aroma components, and can meet the requirement of rapidly analyzing and detecting a large batch of samples.

Preferably, the chromatographic column is an elastic quartz capillary chromatographic column, and the stationary phase is 50% by mass of phenyl-methyl polysiloxane; the specification of the chromatographic column is 60m multiplied by 0.25mm multiplied by 0.25 μm;

preferably, the inlet end of the sample inlet is connected in series with a pre-column; the specification of the pre-column is 5m multiplied by 0.25 mm. The pre-column is an inert quartz capillary column subjected to silanization treatment and has the function of removing part of heavy component impurities in the liquid to be tested so as to improve the accuracy of the test result.

In order to avoid that solid impurities contained in the mixture obtained after the extraction treatment have adverse effects on subsequent analysis, the mixture can be treated by the conventional solid-liquid separation method to obtain an extraction liquid. These solid-liquid separation methods may be methods such as sedimentation, centrifugation, or filtration.

Preferably, the method for detecting the flavor components in the mainstream smoke of the cigarette further comprises the following steps: and extracting the filter disc after the particulate matter is collected by adopting the extraction treatment liquid, and filtering the mixture obtained by extraction treatment to obtain the extraction liquid.

Preferably, the filtration treatment is a treatment using a 0.45 μm organic filtration membrane.

Preferably, the internal standard consists of acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4.

Preferably, the mass ratio of the acetophenone-d 8 to the styrene-d 8 to the benzophenone-d 10 to the diisobutyl phthalate-d 4 is 1-6: 1-6. For example, the mass ratio of acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4 is 1:1:1: 1.

Preferably, the extraction solvent is dichloromethane. The main stream smoke particulate matter trapped by the filter disc is directly extracted by dichloromethane solvent and then analyzed by GC-MS/MS method, the operation is simple and rapid, the flux is high, the cost is low, the solvent consumption is less, the environment is friendly, and the simultaneous extraction and purification of various target objects with larger property difference can be satisfied.

Preferably, the extraction is carried out in an extraction vessel; the extraction container is a cylindrical sealed bottle with the volume of 4-22 mL; the bottle height of the cylindrical sealing bottle is 4-10 cm, and the diameter of the bottle bottom is 1-3 cm.

Preferably, the dihydric alcohol is selected from one or any combination of octanediol, nonanediol and tetradecanediol.

Preferably, the diol consists of octanediol, nonanediol, and tetradecanediol.

Preferably, the mass ratio of the octanediol to the nonanediol to the tetradecanediol is 1-6: 1-6.

More preferably, the mass ratio of the octanediol, the nonanediol and the tetradecanediol is 1:1: 1.

Preferably, the conditions of the gas chromatography in the gas chromatography-tandem mass spectrometry include: the temperature of a sample inlet is 280 ℃; and (5) temperature programming.

Preferably, the temperature programming condition is that the initial temperature is kept at 40 ℃ for 3min, then the temperature is increased to 75 ℃ at the temperature increasing speed of 5 ℃/min, then the temperature is increased to 120 ℃ at the temperature increasing speed of 1 ℃/min, then the temperature is increased to 160 ℃ at the temperature increasing speed of 2 ℃/min, and finally the temperature is increased to 290 ℃ at the temperature increasing speed of 5 ℃/min, and the temperature is kept for 10 min.

More preferably, the conditions of the gas chromatography in the gas chromatography-tandem mass spectrometry include: the temperature of a sample inlet is 280 ℃; the sample injection amount is 0.8-1.0 mu L; the sample introduction mode is non-shunting sample introduction; the carrier gas is helium with the purity of 99.999 percent, and the flow rate is 1.5mL/min in a constant flow mode; the temperature raising procedure is that the initial temperature is kept at 40 ℃ for 3min, then the temperature is raised to 75 ℃ at the temperature raising speed of 5 ℃/min, then the temperature is raised to 120 ℃ at the temperature raising speed of 1 ℃/min, then the temperature is raised to 160 ℃ at the temperature raising speed of 2 ℃/min, and finally the temperature is raised to 290 ℃ at the temperature raising speed of 5 ℃/min, and the temperature is kept for 10 min.

Preferably, the conditions of mass spectrometry in the gas chromatography-tandem mass spectrometry comprise: the ionization mode is electron bombardment ionization; ionization energy is 70 eV; the ion source temperature is 280 ℃; the temperature of the four-level bar is 150 ℃; the temperature of the transmission line is 280 ℃; the scanning mode is dynamic multi-reaction monitoring.

More preferably, the conditions for mass spectrometry in gas chromatography-tandem mass spectrometry comprise: the ionization mode is electron bombardment ionization; ionization energy is 70 eV; the filament current is 35 muA; the ion source temperature is 280 ℃; the temperature of the four-level bar is 150 ℃; the temperature of the transmission line is 280 ℃; the collision gas is nitrogen with the purity of 99.999 percent, and the flow rate is 1.5 mL/min; the quenching gas is helium with the purity of 99.999 percent, and the flow rate is 2.25 mL/min; the scanning mode is dynamic multi-reaction monitoring.

Preferably, the filter is a cambridge filter.

Preferably, the inner diameter of the cambridge filter disc is 44-92 mm, and the thickness of the cambridge filter disc is 1-2 mm.

Preferably, the flavor component is selected from one or any combination of the following compounds:

drawings

FIG. 1 is a total ion flow graph of fragrance components in a standard solution measured in example 1;

FIG. 2 is a total ion flow diagram of the aroma components in the smoke of a cigarette sample obtained by the detection in example 1;

FIG. 3 is a PCA score chart of flavor components in mainstream smoke of cigarette samples of different specifications in Experimental example 2 (PC1 is a principal component factor 1 representing difference by subjecting data to dimensionality reduction treatment, and PC2 is a principal component factor 2 representing difference by subjecting data to dimensionality reduction treatment).

Detailed Description

The technical solution of the present invention will be further explained with reference to the specific embodiments.

The materials used in the examples of the present invention: acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4 were supplied by Sigma-Aldrich, USA; 50% by weight of phenyl-methylpolysiloxane was supplied by Agilent, USA.

The specific embodiment of the method for detecting the flavor components in the mainstream smoke of the cigarette is as follows:

example 1

The method for detecting the flavor components in the mainstream smoke of the cigarette comprises the following steps:

(1) smoking 2-6 cigarettes according to the requirements of GB/T16447-2004 (namely smoking 1 mouth per 60s, smoking capacity of 35mL and smoking duration of 2s), placing the cigarette samples in an environment with the temperature of 22 +/-1 ℃ and the relative humidity of 60 +/-3% for 48h, selecting the cigarette samples with the average mass of +/-0.020 g and the average smoking resistance of +/-30 Pa as test samples, and then trapping particulate matters in the mainstream smoke of the cigarettes by using a Cambridge filter (the inner diameter of the Cambridge filter is 44mm and the thickness of the Cambridge filter is 1 mm).

(2) After the cigarettes are sucked, folding the Cambridge filter disc after the particulate matter is collected, placing the Cambridge filter disc in a 12mL sample bottle (the bottle height of the sample bottle is 8cm, and the bottle bottom diameter is 2cm), adding 10mL of dichloromethane extraction treatment liquid containing an internal standard substance (the internal standard substance comprises acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4 in a mass ratio of 1:1:1: 1) with the concentration of 2.4 mug/mL into the sample bottle, performing ultrasonic extraction for 30min under the condition that the ultrasonic frequency is 40kHz, filtering the mixture obtained by extraction by using a disposable syringe (the syringe is provided with a 0.45-micrometer organic filter membrane), and collecting the filtrate to obtain an extraction liquid.

(3) Adding 1mL of extract into a chromatographic bottle, then adding 50 μ L of dichloromethane solution of an analysis protective agent with the mass concentration of 60mg/mL, and shaking up to obtain a solution to be detected, wherein the analysis protective agent consists of octanediol, nonanediol and tetradecanediol with the mass ratio of 1:1:1, and the concentrations of the octanediol, the nonanediol and the tetradecanediol in the solution to be detected are all 1 mg/mL.

(4) And (5) carrying out GC-MS/MS analysis on the liquid to be detected.

The conditions of the gas chromatography in the GC-MS/MS analysis were: the chromatographic column is an elastic quartz capillary chromatographic column, the stationary phase is phenyl-methyl polysiloxane with the mass fraction of 50%, the specification is 60m multiplied by 0.25mm multiplied by 0.25 mu m, the sample inlet end is connected with a pre-column (5m multiplied by 0.25mm) in series, and the pre-column is an inert quartz capillary column subjected to silanization treatment; the temperature of a sample inlet is 280 ℃; the sample injection amount is 0.8-1.0 mu L; the sample introduction mode is non-shunting sample introduction; the carrier gas is helium with the purity of 99.999 percent, and the flow rate is 1.5mL/min in a constant flow mode; the temperature raising procedure is that the initial temperature is kept at 40 ℃ for 3min, then the temperature is raised to 75 ℃ at the temperature raising speed of 5 ℃/min, then the temperature is raised to 120 ℃ at the temperature raising speed of 1 ℃/min, then the temperature is raised to 160 ℃ at the temperature raising speed of 2 ℃/min, and finally the temperature is raised to 290 ℃ at the temperature raising speed of 5 ℃/min, and the temperature is kept for 10 min.

The conditions of the mass spectrum in the GC-MS/MS analysis were: the ionization mode is electron bombardment ionization; ionization energy is 70 eV; the filament current is 35 muA; the ion source temperature is 280 ℃; the temperature of the four-level bar is 150 ℃; the temperature of the transmission line is 280 ℃; the collision gas is nitrogen with the purity of 99.999 percent, and the flow rate is 1.5 mL/min; the quenching gas is helium with the purity of 99.999 percent, and the flow rate is 2.25 mL/min; the scanning mode is dynamic Multiple Reaction Monitoring (MRM). The parameters of the MRM are shown in Table 1.

TABLE 1 MRM parameters of aroma components and internal standards

Note: ocimene, citral, theaspirane, alpha-damascone, 8-mercaptomaleone, octahydrocoumarin, jasmonic acid, methyl dihydrojasmonate, 3-butylidenephthalide, bisabolol, farnesyl acetone and linalyl benzoate are respectively a mixture consisting of two respective isomers; the benzaldehyde glycerol acetal is a mixture consisting of three isomers of the benzaldehyde glycerol acetal, and the megastigmatrienone is a mixture consisting of four isomers of the megastigmatrienone; in the collision energy column, the left side is the quantitative ion pair collision energy, and the right side is the qualitative ion pair collision energy.

(5) Preparing 5 concentrations of standard solutions containing aroma components in smoke by using dichloromethane as a solvent, adding an internal standard substance and an analysis protective agent, performing GC-MS/MS analysis, performing linear regression analysis by using an internal standard quantitative method, taking the ratio of the peak area of the chromatographic peak of each aroma component in the standard solution to the peak area of the chromatographic peak of the internal standard substance as a vertical coordinate, taking the concentration of each aroma component in the corresponding standard solution as a horizontal coordinate, obtaining a standard curve, and detecting the obtained total ion flow graph of the aroma components in the standard solution as shown in figure 1. Wherein, because the content of the flavor components in the smoke is different, the concentration of the flavor components in the standard solution is selected to be proper 5 concentrations according to the content of the corresponding flavor components in the smoke; the conditions of gas chromatography and the conditions of mass spectrometry in the GC-MS/MS analysis are the same as those in step (4); the concentrations of the analysis protective agents in the 5 kinds of concentration standard solutions are all 3mg/mL, and the analysis protective agents consist of octanediol, nonanediol and tetradecanediol in a mass ratio of 1:1: 1; the concentrations of the internal standard substances in the standard solutions with 5 concentrations are all 2.4 mug/mL, and the internal standard substances consist of acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4 in a mass ratio of 1:1:1: 1.

(6) The total ion flow diagram of the aroma components in the smoke sample obtained by detection is shown in figure 2, and the content of the aroma components detected in the smoke of the cigarette sample is shown in table 2.

TABLE 2 content of aroma components detected in cigarette sample smoke

Example 2

The present example is different from example 1 only in that cigarette samples were changed to 6 different brands of cigarette samples to detect the flavor components in a wider variety of mainstream smoke, the 6 different brands of cigarette samples were respectively Yanan hong Yun, Furong, Haomai, Liqu, Suyan and Lanzhou, numbered A, B, C, D, E and F in sequence, and the content of the flavor components detected in the 6 different brands of cigarette samples smoke is shown in Table 3.

TABLE 36 content of aroma components detected in cigarette sample smoke of different brands

Experimental example 1

1. Sensitivity of the reaction

Preparing 5 concentrations of standard solutions containing aroma components in smoke by using dichloromethane as a solvent, adding an internal standard substance and an analyte protective agent, performing GC-MS/MS analysis, and performing linear regression analysis by using an internal standard quantitative method, taking the ratio of the peak area of the chromatographic peak of each aroma component in the standard solution to the peak area of the chromatographic peak of the internal standard substance as a vertical coordinate, and taking the concentration of each aroma component in the corresponding standard solution as a horizontal coordinate to obtain a standard curve. Wherein, the conditions of gas chromatography and mass spectrometry in the GC-MS/MS analysis are the same as those in example 1; the concentrations of the analysis protective agents in the 5 kinds of concentration standard solutions are all 3mg/mL, and the analysis protective agents consist of octanediol, nonanediol and tetradecanediol in a mass ratio of 1:1: 1; the concentrations of the internal standard substances in the standard solutions with 5 concentrations are all 2.4 mug/mL, and the internal standard substances consist of acetophenone-d 8, styrene-d 8, benzophenone-d 10 and diisobutyl phthalate-d 4 in a mass ratio of 1:1:1: 1. The standard curve of each flavor component has good linear relation and linear correlation coefficient (R)²) Between 0.9856 and 0.9999, wherein R of most of the fragrance components²>0.99, as shown in table 4.

To the cambridge filter from which the cigarette smoke was collected in example 1, 50 μ L of each of the 3 concentration (0.2, 1 and 5mg/mL) levels of standard solutions was added, and then the content of flavor components in the mainstream smoke of the cigarette samples was measured according to the method of example 1, and the normalized recovery rate was calculated. As shown in table 4, the average recovery rate of most of the flavor components at the three addition levels was 70% to 120%.

The detection Limit (LOD) was calculated as 3 times the signal-to-noise ratio and the quantification Limit (LOQ) was calculated as 10 times the signal-to-noise ratio. As shown in Table 4, the detection limit of all the aroma components is between 0.2 ng/mL and 54.2ng/mL, the quantification limit is between 0.7ng/mL and 180.7ng/mL, and the quantification limit of most aroma components is less than 100 ng/mL.

TABLE 4 Linear correlation coefficient, recovery, detection limit and quantitation limit of flavor components

2. Precision and stability

A total of 79 flavor components were detected in the mainstream smoke of a laboratory reference cigarette (without flavoring) according to the method of example 1, and these flavor components were derived from the tobacco leaf combustion, pyrolysis, thermal reaction and heat transfer pathways, while the other 213 flavor components were detected only in each commercially available flavored cigarette and were transferred from the flavoring to the mainstream smoke.

In order to measure all the flavor components simultaneously, the cambridge filter disc is used for trapping particulate matters in the mainstream smoke of 5 laboratory reference cigarettes (without adding spices) according to the method of example 1, then 213 mixed standard solutions of undetected flavor components are dripped on the surface of the cambridge filter disc after trapping the particulate matters, the addition amount of the 213 flavor components is 1 mug, namely the addition amount is equivalent to 200 ng/cigarette, then the content of the flavor components in the mainstream smoke is detected according to the method of example 1, the test precision in the day is evaluated through the Relative Standard Deviation (RSD) obtained by carrying out 5 parallel test experiments in one day, the test precision in the day is evaluated through the Relative Standard Deviation (RSD) obtained by carrying out 1 parallel test experiment in 5 days continuously, and the test results are shown in Table 5. As is apparent from Table 5, the daily test precision and the daytime test precision were 0.3 to 22.5% and 1.0 to 30.2%, respectively, and the daily test precision for most of the flavor components was 10% or less. Experimental results show that the detection method has good precision and good stability, and can meet the analysis and detection requirements.

TABLE 5 Intra-and diurnal measurement precision of fragrance ingredients

Experimental example 2

The method of example 1 was used to measure the content of flavor components in mainstream smoke of 3 different specifications of fine, medium and regular cigarette samples provided by cigarette industry Limited liability company in Yunnan, the physical parameters of the 3 different specifications of cigarette samples are shown in Table 6, and 6 parallel measurements were performed on each specification of cigarette. The 86 flavor components are detected in the smoke, the PCA analysis is carried out on the content results of the 86 detected flavor components through SIMCA software, the result is within a 95% confidence interval, and the score chart of the PCA analysis is shown in figure 3, wherein three data points of the fine cigarette sample are superposed together. The sum of the two principal component factors PC1 and PC2 represents 96.2% of the total difference, the prediction capability is 88.9% (Q2(cum) ═ 0.889), and the model has better discriminability. As can be seen from FIG. 3, the smoke of the cigarettes with different specifications shows significant differences in the score maps, and 26 components with significant differences (with the P value less than 0.01 as the standard) are screened by adopting the t test, and the results are shown in Table 7.

TABLE 63 physical parameters of cigarette samples of different specifications

Physical parameters	Fine cigarette	Middle cigarette	Conventional cigarette
				Cigarette weight/mg	5.66	6.85	9.46
Suction resistance/Pa	1414	1365	1130
				Filter ventilation rate/%)	33.3	12.5	0
Ventilation rate of tobacco branch section	10.8	7.5	9.6
				Total ventilation rate/%)	44.1	20	9.6
Circumference/mm	16.8	20.04	24.19
				Cigarette length/mm	99.39	88.28	84.04

P values for the different compounds in Table 726

Serial number	Compound (I)	p value	Serial number	Compound (I)	p value
						1	Para-methyl benzaldehyde	<0.001	14	6-methyl-3, 5-heptadien-2-one	0.005
2	Meta-methyl benzaldehyde	<0.001	15	3, 4-dehydro-beta-ionones	0.005
						3	O-methylbenzaldehyde	<0.001	16	Beta-damascenone	0.004
4	3-methyl-2-cyclopentenone	<0.001	17	P-methylacetophenone	0.002
						5	5-methylfurfural	<0.001	18	Maltol	0.001
6	2-cyclohexen-1-ones	<0.001	19	4- (2-furyl) -3-buten-2-one	0.001
						7	Methylthiopropanal	<0.001	20	Beta-damascone	0.001
8	2-methyl-2-cyclopenten-1-one	<0.001	21	3, 4-hexanedione	<0.001
						9	Cyclohexanone	<0.001	22	4-oxo-isophorone	<0.001
10	2-cyclopenten-1-ones	<0.001	23	3-methylacetophenone	<0.001
						11	Beta-acetonaphthones	0.031	24	Beta-cyclohomocitral	<0.001
12	Saffron aldehyde	0.016	25	Veratraldehyde	<0.001
						13	Zingerone	0.008	26	Syringaldehyde	<0.001