阅读说明：本技术 酶活性提高的逆转录酶及其应用 (Reverse transcriptase having improved enzymatic activity and use thereof ) 是由 刘欢欢 郭娜 李慧真 张周刚 韩鸿雁 郭苗苗 郑越 董宇亮 章文蔚 徐崇钧 于 2018-12-26 设计创作，主要内容包括：本发明涉及一种逆转录酶及其应用。该逆转录酶与野生型的M-MLV逆转录酶相比,具有R450H等突变位点。该逆转录酶具有提高的聚合酶活性、提高的热稳定性和降低的RNase H活性。(The invention relates to a reverse transcriptase and application thereof. Compared with wild M-MLV reverse transcriptase, the reverse transcriptase has mutation sites such as R450H. The reverse transcriptase has increased polymerase activity, increased thermostability and reduced RNase H activity.)