阅读说明：本技术 一种用于骨质疏松性骨折快速愈合的药物组合物 (Pharmaceutical composition for rapidly healing osteoporotic fracture ) 是由 孙鹏 韩亮 邢春叶 于 2021-10-20 设计创作，主要内容包括：本发明属于西药技术领域,公开了一种用于骨质疏松性骨折快速愈合的药物组合物,该药物组合物为口服制剂,所述口服制剂由马来酸曲美布汀、双膦酸盐类和药剂学上可接受的辅料制备而成。本发明通过马来酸曲美布汀在体内提高双膦酸盐类促进骨形成的生物活性,同时降低破骨细胞的功能活性,抑制骨吸收活动,从而协同性提高骨密度,促进骨质疏松性骨折的快速愈合。(The invention belongs to the technical field of western medicines, and discloses a pharmaceutical composition for rapidly healing osteoporotic fracture. The invention improves the biological activity of bisphosphonates in vivo to promote bone formation, reduces the functional activity of osteoclast and inhibits bone absorption activity, thereby synergistically improving bone density and promoting the rapid healing of osteoporotic fracture.)

1. The pharmaceutical composition for rapidly healing osteoporotic fracture is characterized by being an oral preparation, wherein the oral preparation is prepared from bisphosphonate medicines, trimebutine maleate and pharmaceutically acceptable auxiliary materials.

2. The pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 1, wherein said bisphosphonate drug is selected from one or more of the following: alendronate sodium, risedronate sodium, zoledronic acid, ibandronate sodium, pamidronate disodium, etidronate disodium, and clodronate disodium.

3. The pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 2, wherein said bisphosphonate is alendronate sodium, and the weight ratio of alendronate sodium to trimebutine maleate is 1: 15-60.

4. The pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 3, wherein the weight ratio of alendronate sodium to trimebutine maleate in said pharmaceutical composition is 1: 20-40.

5. The pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 4, wherein the weight ratio of alendronate sodium to trimebutine maleate in said pharmaceutical composition is 1: 40.

6. the pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 1, wherein said oral preparation is tablet, capsule, granule, dry suspension, pill, etc.

7. The pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 6, wherein said oral formulation is dispersible tablet.

8. The pharmaceutical composition for rapid healing of osteoporotic fracture according to claim 7, wherein said pharmaceutically acceptable excipients include starch, lactose, hypromellose, tartaric acid, sodium carboxymethyl starch, colloidal silicon dioxide, magnesium stearate.

Technical Field

The invention belongs to the technical field of western medicines, relates to a medicine for healing bone wounds, and particularly relates to a medicine composition for quickly healing osteoporotic fracture.

Background

Osteoporosis is a systemic bone disease in which bone density and bone quality decrease due to various causes, bone microarchitecture is destroyed, bone fragility increases, and thus fractures easily occur [ CHENGWH, MICLAU T, CHOW SKH, et al.Fracture healing in osteoporotic bone.Injury.2016; 47(Suppl 2): S21-S26 ]. According to the physiological and anatomical standards, the bone tissue is composed of cells, fibers and matrix, belongs to a connective tissue with higher density and higher hardness, and the elastic property of the bone tissue allows the bone tissue to deform to a certain degree under the condition of bearing load. The biomechanical properties of bone tissue depend on bone density and trabecular bone distribution and are also affected by osteoporosis. The bone tissues can be divided into cancellous bone and cortical bone, wherein the cancellous bone has a highly regular reticular structure, and has lower density, lower mechanical strength and stronger elasticity and anisotropic biomechanical property; the cortical bone has highly regular parallel plate layer units, high density, high mechanical strength, and weak elasticity and anisotropic biomechanics (Linhua et al. guide for intervention in osteoporotic fracture perioperative period [ J ]. J. China osteoporosis and bone and mineral diseases, 2018,11(5): 438-448.).

After osteoporosis occurs, new bone formation is less than bone resorption, and negative balance is present. With the increase of osteoporosis degree, the negative balance becomes more serious, the cortical bone becomes thinner, the mechanical strength is reduced, and when a certain external force is applied, the osteoporotic fracture can occur. Osteoporosis is a common chronic skeleton disease of the whole body of the elderly, and along with the aging degree of the Chinese population, the scale of people with osteoporosis fracture gradually increases, so how to improve the treatment effect and improve the healthy living standard of people is being paid attention to by clinicians.

Aiming at the clinical treatment of osteoporotic fracture, anti-osteoporosis treatment is required to be strengthened besides three stages of fracture reduction (closing or incision), fixation (external fixation or internal fixation), functional exercise and functional rehabilitation. In the post-fracture clinical treatment process, anti-osteoporosis treatment is continuously carried out, so that the bone mass can be improved, the bone quality can be improved, and the risk of re-fracture can be reduced. Therefore, after an osteoporotic fracture occurs, both the treatment of the fracture site and the treatment of osteoporosis are equally important. Research shows that the medicine can raise bone quality of fracture part and speed fracture healing.

Currently, agents for preventing and treating osteoporosis can be classified into basic supplements and agents for inhibiting osteoclasts, promoting osteoblasts, or modulating osteoclasts and osteoblasts simultaneously.

First, basic supplements are calcium and vitamin D, whose main action is to promote bone mineralization and thus reduce the risk of hip and non-spinal fractures, however these supplements must be taken for long periods of time and only prophylactically and, therefore, the therapeutic effect of osteoporotic fractures is insignificant.

Secondly, the strontium salt acts on a calcium sensitive receptor of the osteocyte, regulates the growth, differentiation and apoptosis of the osteoblast and the osteoclast, inhibits bone resorption, promotes osteogenesis and finally increases bone density. For example, alendronate sodium has the dual effects of promoting bone formation and inhibiting bone resorption, can reduce the occurrence risk of vertebral body, non-vertebral body and hip fracture, but because the administration dosage of the drug is larger, the clinical recommended dosage is more than 2000mg per day, and the long-term use can increase the risk of postmenopausal myocardial infarction.

In addition, teriparatide is the first parathyroid hormone analogue, provides a lower-cost treatment scheme for patients with severe osteoporosis and high fracture risk, and is the first approved bone formation promoter. The medicine enhances the osteoblast activity of patients by small dose and intermittent administration, increases the bone mineral content and bone density, further remarkably reduces the incidence rate of vertebral body and non-vertebral body fractures, has common clinical adverse reactions of leg spasm pain, headache, dizziness and nausea, and can also cause hypercalcemia after long-term use, so that the patients with high blood calcium dose do not use teriparatide as much as possible.

In addition, bisphosphonates are a class of medicines for resisting metabolic bone diseases developed in the last two decades and are used for treating osteolytic lesions caused by various factors, the action mechanism of the bisphosphonates for treating osteoporosis is to prevent bone resorption, increase bone density and effectively reduce the risk of hip, vertebra and non-vertebra fractures, however, the curative effect of the bisphosphonates for treating osteoporotic fractures is not ideal.

Disclosure of Invention

In view of the clinical problems of insufficient bone fusion degree, slow bone formation and the like existing when the implant is fixed and placed in a simple operation mode in the treatment of osteoporotic fracture, the invention aims to provide a pharmaceutical composition for quickly healing the osteoporotic fracture so as to solve the technical problems.

In order to achieve the technical purpose, the inventor considers that patients with osteoporotic fracture are mainly elderly, most of the people are often accompanied by basic diseases, the physical health condition of the patients is worse than that of normal people, and more problems are often caused in the treatment of osteoporotic fracture, for example, most of the elderly also have a plurality of complicated diseases such as cardiovascular and cerebrovascular diseases, diabetes, respiratory diseases and the like. In addition, under the influence of osteoporosis, the bone reconstruction of a patient is unbalanced, stress stimulation at a fracture part is insufficient, and the healing time is generally longer. Therefore, there is a need for drug intervention following surgical treatment, while considering that the selected drugs do not exacerbate the underlying disease in the elderly.

The inventor finds that in the clinical treatment process, one elderly fracture patient with spleen-stomach weakness type functional dyspepsia takes alendronate sodium and trimebutine maleate for several days in a hospital, the fracture healing speed of the elderly fracture patient is obviously higher than that of other elderly patients, and the bone density condition of the elderly fracture patient is detected by an X-ray detector, so that the callus bone density of the elderly fracture patient is greatly improved, and the osteoporosis is obviously improved. Therefore, the inventor carries out pharmacodynamic animal experiments by taking alendronate sodium and trimebutine maleate as test substances, further proves that the trimebutine maleate has the bioactivity of improving alendronate sodium and promoting bone formation in vivo, and finally obtains the following technical scheme:

the pharmaceutical composition is an oral preparation, and the oral preparation is prepared from bisphosphonate medicines, trimebutine maleate and pharmaceutically acceptable auxiliary materials. Further preferably, the pharmaceutical composition for rapid healing of osteoporotic fracture, wherein the bisphosphonate drugs are selected from one or more of the following: alendronate sodium, risedronate sodium, zoledronic acid, ibandronate sodium, pamidronate disodium, etidronate disodium, and clodronate disodium.

Further preferably, the pharmaceutical composition for rapid healing of osteoporotic fracture as described above, wherein the bisphosphonate is preferably a third generation drug alendronate sodium, and the weight ratio of alendronate sodium to trimebutine maleate is 1: 15-60. Still further preferably, the weight ratio of alendronate sodium to trimebutine maleate is 1: 20-40. In pharmacodynamic experiments, although the trimebutine-alendronate sodium low-dose group has a remarkable promoting effect on bone density and maximum load indexes of rat thighbone, the improvement effect on the bending strength of the thighbone is not as ideal as that of the trimebutine-alendronate sodium high-dose group, so that the dose ratio of the trimebutine sodium to the maleic acid trimebutine in the invention is preferably 1: 40.

In addition, as the alendronate sodium and trimebutine maleate can achieve obvious drug effect through an oral administration mode, the pharmaceutical composition for rapidly healing the osteoporotic fracture is preferably an oral preparation, and the oral preparation can be tablets, capsules, granules, dry suspensions, pills and the like. Because patients with osteoporosis fracture are mainly elderly people who often have swallowing difficulty, the oral preparation selected by the invention is mainly dispersible tablets, granules and dry suspension. Most preferred are dispersible tablets, because they are easy to produce and package, and they have high stability, are suitable for long-term storage, and can be rapidly disintegrated and dispersed in milk or water, and are convenient for the elderly to take. When preparing the dispersible tablet, pharmaceutically acceptable adjuvants including starch, lactose, hypromellose, tartaric acid, sodium carboxymethyl starch, colloidal silicon dioxide, and magnesium stearate are added.

For patients with osteoporotic fracture, the choice of anti-osteoporosis drugs is mainly based on whether the anti-osteoporosis effect is achieved, but whether the anti-osteoporosis drugs have positive significance on the progress of fracture healing. Compared with normal organisms, osteoporotic individuals have poorer bone histology foundation, the internal fixation holding power is reduced, and the healing process after fracture is delayed compared with that of normal individuals.

Detailed Description

The advantages and features of the present invention will become more apparent from the following description, which proceeds with reference to specific test examples. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit of the invention, and it is intended to cover all such changes and modifications as fall within the scope of the invention.

48 SD male rats weighing 260-300 g are divided into a blank control group, a model control group, a trimebutine group, an alendronate sodium group, a trimebutine-alendronate sodium low-dose group and a trimebutine-alendronate sodium high-dose group according to a random digital table method, and each group comprises 8 rats.

Except for the blank control group, rats in other groups are gavaged with tretinoin 70mg/kg 1 time per day, and the rats are caused to have osteoporosis after being administered with tretinoin for 2 weeks. Detecting bone density, after confirming that an osteoporosis SD rat model is successfully established, carrying out intraperitoneal injection anesthesia on all rats including a blank control group, carrying out hair removal on right hind limbs under an aseptic condition, carrying out iodine tincture disinfection, carrying out incision about 2cm in length on right femoral facial skin under the aseptic condition, carrying out blunt separation on muscles, exposing femurs, carrying out sharp and annular stripping on 1cm of periosteum in the middle sections of the femurs, using a osteotome to cause transverse fracture, then carrying out internal fixation through an adult metacarpal and phalangeal bone micro bone fracture plate, and suturing and bandaging wounds layer by layer after a good fixation effect is achieved. The SD rats are then returned to the rearing cage for free movement and are normally reared.

After the model building is successful, on the basis of the body mass of a rat, a trimebutine group is perfused with 20mg/kg of trimebutine maleate, an alendronate sodium group is perfused with 0.5mg/kg of alendronate sodium, a trimebutine-alendronate sodium low-dose group is perfused with 10mg/kg of trimebutine maleate and 0.5mg/kg of alendronate sodium, a trimebutine-alendronate sodium high-dose group is perfused with 20mg/kg of trimebutine maleate and 0.5mg/kg of alendronate sodium, a blank control group and a model control group are perfused with purified water, and each group is perfused with 1 time a day and continuously administrate for 5 weeks.

After the dosing was completed, rats were anesthetized with 10% chloral hydrate and sacrificed for femoral biomechanical analysis, and the maximum load and bending strength were determined. In addition, the bone density of femoral callus of rat was measured by a dual-energy X-ray densitometer, and the scanning was performed with the fracture end 2mm × 2mm as the center region in a scanning mode of 1mm × 1mm resolution and 60mm/s scanning speed, and the measurement was performed 5 times continuously. The experimental statistics are shown in table 1.

TABLE 1 maximum femoral load, flexural strength, bone density of rats in each group

Compared with the blank control group, the composition of the composition,^*p is less than 0.05; compared with the model control group,^#p is less than 0.05; compared with the trimebutine group,^▲p is less than 0.05; compared with the sodium alendronate group,^■P＜0.05

as can be seen from table 1, in the aspect of callus bone density, the model control group is significantly reduced compared with the blank control group, and the differences have statistical significance (P < 0.05), the alendronate sodium group is improved, but still has significant differences (P < 0.05) compared with the blank control group, and the trimebutine-alendronate sodium low and high dose groups both greatly improve the callus bone density, and not only have significant differences (P < 0.05) compared with the model control group, but also have statistical differences (P < 0.05) compared with the trimebutine group or the alendronate sodium group. The experimental result shows that trimebutine maleate is helpful to improve the bioactivity of alendronate sodium for promoting bone formation and increase the bone density of osteoporosis rats in vivo, and has a certain dose dependence relationship. In addition, in the aspect of maximum load and bending strength of the thighbone of a rat, the statistical result of indexes of each group is similar to the bone density index, but the improvement effect of the low-dose group of the trimebutine-alendronate sodium on the bending strength of the thighbone is not ideal enough, which shows that the dose ratio of the alendronate sodium to the trimebutine maleate is preferably 1:40 when the osteoporosis fracture is treated.