阅读说明：本技术 一种低聚原花青素的眼用产品制备方法及应用 (Preparation method and application of oligomeric proanthocyanidins ophthalmic product ) 是由 陈蔚 姜丹 郑钦象 于 2021-08-19 设计创作，主要内容包括：本发明公开了一种低聚原花青素的眼用产品制备方法及应用,涉及眼部护理技术领域,制备方法包括S1、原花青素低聚物提取：将葡萄籽挤压成汁,再将挤压后的残渣采用乙醇提取,提取后挥发乙醇,得到溶液A,将挤压的葡萄籽油与溶液A混合后用超临界CO-(2)萃取原花青素低聚物；S2、配制基础液：用多种植物混合后提取制成基础液,S3、将基础液与原花青素以及维生素E、维生素A混合制备眼用液；本发明所制备的眼用液,不仅可以当做眼药水,还可以外敷用于眼部及眼部周围皮肤的护理,改善眼部皱纹、延缓皮肤衰老。(The invention discloses a preparation method and application of an ophthalmic product of oligomeric proanthocyanidins, relates to the technical field of eye care, and discloses a preparation methodThe method comprises the following steps of S1, procyanidine oligomer extraction: squeezing grape seed to obtain juice, extracting the residue with ethanol, volatilizing ethanol to obtain solution A, mixing squeezed grape seed oil with solution A, and supercritical CO 2 Extracting the oligomeric proanthocyanidins; s2, preparing a base solution: mixing multiple plants, extracting to obtain base solution, S3, mixing the base solution with procyanidine, vitamin E and vitamin A to obtain eye solution; the eye drops prepared by the invention can be used as eye drops, and can be externally applied to nursing eyes and skin around the eyes, improving wrinkles of the eyes and delaying skin aging.)

1. A method for preparing an ophthalmic product of oligomeric proanthocyanidins, comprising the steps of:

s1: oligomeric proanthocyanidin extraction

S1-1: mechanically extruding grape seeds, collecting exuded grape seed oil for later use, adding grape seed residues and 70% ethanol solution at a mass ratio of 1:2 into an extractor for extraction, and filtering after extraction to obtain grape seed extract;

s1-2: heating the grape seed extracting solution obtained in the step S1-1 at the temperature of 60-65 ℃, stirring to volatilize the ethanol solution to obtain a solution A, and mixing and stirring the solution A and grape seed oil to obtain a solution B;

s1-3: subjecting the mixed solution B to supercritical CO₂Extracting, and separating procyanidine oligomer with the purity of 96.5% by a separator after extraction;

s2: preparing base liquid

Taking the dried medicines in parts by weight: 3-4 parts of ginseng, 6-8 parts of wild chrysanthemum, 5-6 parts of sowthistle tasselflower herb, 3-5 parts of mint, 4-5 parts of witch hazel and 3-5 parts of sea-core kernel are mixed and then placed into a crusher for crushing to obtain mixed powder, the mixed powder is poured into a boiler for water extraction, the mass ratio of the mixed powder to water is 1:50, the extraction temperature is 100-120 ℃, the extraction time is 2-3 hours with stirring, the filtrate is obtained after the stirring is finished and is filtered, and then the filtrate is naturally cooled to 5-15 ℃ to obtain a base solution;

s3: preparing eye liquid

Dripping the procyanidin oligomer, the vitamin E and the vitamin A obtained in the step S1 into the base solution, and stirring at a stirring speed of 80r/min for 5-10min to obtain the ophthalmic solution, wherein the mass percent of the procyanidin oligomer in the ophthalmic solution is 1.2-1.4%, the mass percent of the vitamin E is 1.3-1.5%, the mass percent of the vitamin A is 0.8-1.1%, and the balance is the base solution.

2. The method of claim 1, wherein the rotation speed of the extractor in step S1-1 is 1200-3000r/min, and the extraction time is 3-6 min.

3. The method of preparing an ophthalmic product comprising oligomeric procyanidins of claim 1, wherein the supercritical CO is₂Extraction of CO₂The flow is 8-10L/h, the temperature is 50-70 ℃, the extraction pressure is 20-40MPa, the pressure in the first-stage separation kettle is 8-10MPa, the pressure in the second-stage separation kettle is 0.1-0.2MPa, and the extraction time is 1-2 h.

4. The method for preparing an ophthalmic product containing oligomeric proanthocyanidins of claim 1, wherein the particle size of the mixed powder crushed in the step S2 is 96-120 μm.

5. The method of preparing an ophthalmic product of oligomeric procyanidin of claim 1, wherein the stirring speed during the extraction in the step S2 is 30-50 r/min.

6. The method for preparing an ophthalmic product of oligomeric procyanidin of claim 1, wherein the filtering of step S2 is performed by industrial filter cloth.

7. The method of preparing an ophthalmic product containing oligomeric proanthocyanidin as claimed in claim 1, wherein the mechanical pressing pressure in step S1-1 is 10-20MPa, the pressing frequency is 12 times/min, the pressing time is 5-6min, and the pressing surface is changed every pressing.

8. The method for preparing an ophthalmic product of oligomeric procyanidin of claim 1, wherein the stirring speed in step S1-2 is 50-70r/min, and the volatilization time is 3-4 h.

9. The use of an ophthalmic product of oligomeric procyanidins as claimed in any one of claims 1 to 8, wherein the ophthalmic solution obtained in step S3 is instilled as an eyedrop into eyes for relieving eyestrain and dry eyes.

10. The use of an ophthalmic product of oligomeric procyanidins as claimed in any one of claims 1 to 8, wherein the ophthalmic solution of step S3 is used for moisturizing the skin around the eyes and delaying the aging of the skin around the eyes.

Technical Field

The invention relates to the technical field of eye care, in particular to a preparation method and application of an oligomeric proanthocyanidin ophthalmic product.

Background

Procyanidins, a generic term for a large group of polyphenols that are widely present in plants, have the common feature that anthocyanins can be produced by heating in an acidic medium, and are therefore called procyanidins. Procyanidins are a pigment component in plants and are widely present in various plants.

Procyanidine is a general name of a large class of polyphenol compounds widely existing in plants, has strong antioxidation and free radical elimination effects, can effectively eliminate superoxide anion free radicals and hydroxyl free radicals, also participates in metabolism of phosphoric acid and arachidonic acid and protein phosphorylation, and protects lipid from peroxidation damage; is a powerful metal chelating agent which can chelate metal ions and form inert compounds in vivo; protect and stabilize vitamin C, and is helpful for the absorption and utilization of vitamin C. Procyanidine is widely distributed, exists in skins, shells, seeds, kernels, flowers and leaves of many plants, and has the highest content and abundant types of procyanidine in grape seeds.

The morbidity of international dry eye is 5.5% -33.7%, the incidence rate of China is 21% -30%, dry eye patients in China account for more than 30% of outpatients of ophthalmology, and the prevalence rate of dry eye is in a rapid growing state with the aging of social population and the rapid and wide popularization of video terminal equipment. 3.6 million people were analyzed to develop dry eye in 2019. Ocular surface diseases are closely related to ocular surface microenvironment disorder caused by exposure of the ocular surface to air and external environment, and eye drops aiming at oxidative damage of the ocular surface are widely applied to diseases such as dry eye, conjunctivitis and the like. The procyanidine has strong antioxidation and free radical elimination effects, and can be used as an important component for preparing eye drops with oxidative damage to the ocular surface.

Existing ophthalmic products, basically, comprise an ophthalmic solution and a skin care product applied on the eyelid. In daily life, the use frequency of the antioxidant eye drops is not high. However, under the stimulation of the existing electronic equipment, people are not accustomed to eyes more and more, and patients with dry eyes and video terminal syndrome are more and more. People now gradually realize the importance of eye health and skin anti-aging care, but eye products which can effectively protect skin and resist oxidation are lacked in the market.

Disclosure of Invention

In order to solve the technical problems, the invention provides a preparation method and application of an ophthalmic product of oligomeric proanthocyanidins.

The technical scheme of the invention is as follows: a method for preparing an ophthalmic product of oligomeric proanthocyanidins comprises the following steps:

s1: oligomeric proanthocyanidin extraction

S1-1: wrapping grape seeds with gauze, mechanically extruding the grape seeds through the gauze, collecting grape seed oil exuded from the gauze for later use after extrusion is finished, adding grape seed residues and 70% ethanol solution into an extractor according to the mass ratio of 1:2 for extraction, and filtering after extraction is finished to obtain grape seed extracting solution;

s1-2: heating the grape seed extracting solution obtained in the step S1-1 at the temperature of 60-65 ℃, stirring to volatilize the ethanol solution to obtain a solution A, and mixing and stirring the solution A and grape seed oil to obtain a solution B;

s1-3: subjecting the mixed solution B to supercritical CO₂Extracting, and separating procyanidine oligomer with the purity of 96.5% by a separator after extraction;

s2: preparing base liquid

Taking the dried medicines in parts by weight: 3-4 parts of ginseng, 6-8 parts of wild chrysanthemum, 5-6 parts of sowthistle tasselflower herb, 3-5 parts of mint, 4-5 parts of witch hazel and 3-5 parts of sea-core kernel are mixed and then placed into a crusher for crushing to obtain mixed powder, the mixed powder is poured into a boiler for water extraction, the mass ratio of the mixed powder to water is 1:50, the extraction temperature is 100-120 ℃, the extraction time is 2-3 hours with stirring, the filtrate is obtained after the stirring is finished and is filtered, and then the filtrate is naturally cooled to 5-15 ℃ to obtain a base solution;

s3: preparing eye liquid

Dripping the procyanidin oligomer, the vitamin E and the vitamin A obtained in the step S1 into the base solution, and stirring at a stirring speed of 80r/min for 5-10min to obtain the ophthalmic solution, wherein the mass percent of the procyanidin oligomer in the ophthalmic solution is 1.2-1.4%, the mass percent of the vitamin E is 1.3-1.5%, the mass percent of the vitamin A is 0.8-1.1%, and the balance is the base solution.

Further, the rotation speed of the extractor in the step S1-1 is 1200-3000r/min, the extraction time is 3-6min, the extraction speed is high, and the extraction effect is good.

Further, the supercritical CO₂Extraction of CO₂The flow is 8-10L/h, the temperature is 50-70 ℃, the extraction pressure is 20-40MPa, the pressure in the first-stage separation kettle is 8-10MPa, the pressure in the second-stage separation kettle is 0.1-0.2MPa, the extraction time is 1-2h, and the procyanidine extraction efficiency is high under the above parameters.

Further, the particle size of the mixed powder crushed in the step S2 is 96-120 μm, so that the medicinal components can be effectively leached.

Further, the stirring speed at the time of extraction in the step S2 is 30 to 50r/min, and the extracted base solution has a good drug effect.

Further, in the step S2, industrial filter cloth is used for filtering, so that the industrial filter cloth has a good filtering effect.

Further, in the step S1-1, the mechanical extrusion pressure is 10-20MPa, the extrusion surface needs to be changed every time of extrusion, the extrusion frequency is 12 times/min, the extrusion time is 5-6min, the extrusion surface needs to be changed every time of extrusion, and the gauze is tightened to fully leach out the liquid oil in the grape seeds.

Further, the stirring speed in the step S1-2 is 50-70r/min, the volatilization time is 3-4h, and the ethanol is fully volatilized.

Further, the ophthalmic solution obtained in step S3 is dropped into the eyes as an eyedrop for relieving eyestrain and dry eyes.

Further, the ophthalmic solution described in step S3 is used for wet dressing of skin around the eyes, for moisturizing the skin around the eyes and for delaying aging of the skin around the eyes.

The invention has the beneficial effects that:

(1) the eye drop prepared by the invention can be dropped into eyes to be used as an eye drop, can effectively relieve eye dryness and eye fatigue, has the capacity of removing oligomeric proanthocyanidins and stronger antioxidant capacity, has good treatment effect on diseases related to oxidative damage on the ocular surface, can obviously reduce oxidative damage, and can effectively reduce apoptosis and tissue damage.

(2) The eye liquid prepared by the method can be used as eye drop for carrying out anti-oxidation treatment on eyes, and can also be externally applied for nursing eyes and skin around the eyes. The eye liquid has strong oxidation resistance, so that the eye liquid can well supplement water, improve eye wrinkles and delay eye skin aging when used for eye skin care.

Drawings

FIG. 1 is a flow chart of the preparation of ophthalmic solutions of the present invention.

FIG. 2 is a graph showing the antioxidant assay of the ophthalmic solution of the present invention.

Detailed Description

Example 1

As shown in fig. 1, a method for preparing an ophthalmic product of oligomeric procyanidin comprises the following steps:

s1: oligomeric proanthocyanidin extraction

S1-1: wrapping grape seeds with gauze, mechanically extruding the grape seeds through the gauze, collecting grape seed oil exuded from the gauze for later use after extrusion is finished, adding grape seed residues and 70% ethanol solution into an extractor according to the mass ratio of 1:2 for extraction, and filtering after extraction is finished to obtain grape seed extracting solution;

s1-2: heating the grape seed extracting solution obtained in the step S1-1 at the temperature of 60 ℃, stirring to volatilize the ethanol solution to obtain a solution A, and mixing and stirring the solution A and grape seed oil to obtain a solution B;

s1-3: subjecting the mixed solution B to supercritical CO₂Extracting, and separating with a separator to obtain extract with a purity of 96.5%Oligomeric proanthocyanidins;

s2: preparing base liquid

Taking the dried medicines in parts by weight: mixing 3 parts of ginseng, 6 parts of wild chrysanthemum flower, 5 parts of sowthistle tasselflower herb, 3 parts of mint, 4 parts of witch hazel and 3 parts of sea nucleolus, crushing the mixture in a crusher to obtain mixed powder, pouring the mixed powder into a boiler to extract water, wherein the mass ratio of the mixed powder to the water is 1:50, the extraction temperature is 100 ℃, the extraction time is 2 hours with stirring, filtering the mixture after the stirring is finished to obtain filtrate, and naturally cooling the filtrate to 5 ℃ to obtain a basic solution;

s3: preparing eye liquid

And (4) dripping the procyanidin oligomer obtained in the step (S1), vitamin E and vitamin A into the base solution, and stirring for 5min at a stirring speed of 80r/min to obtain the ophthalmic solution, wherein the mass percent of the procyanidin oligomer in the ophthalmic solution is 1.2%, the mass percent of the vitamin E in the ophthalmic solution is 1.3%, the mass percent of the vitamin A in the ophthalmic solution is 0.8%, and the balance is the base solution.

In the step S1-1, the rotation speed of the extractor is 1200r/min, the extraction time is 3min, the extraction speed is high, and the extraction effect is good.

Supercritical CO₂Extraction of CO₂The flow is 8L/h, the temperature is 50 ℃, the extraction pressure is 20MPa, the pressure in the first-stage separation kettle is 8MPa, the pressure in the second-stage separation kettle is 0.1MPa, the extraction time is 1h, and the procyanidine extraction efficiency is high under the above parameters.

The particle size of the crushed mixed powder in the step S2 is 96-100 mu m, so that the medicinal components are effectively leached.

In the step S2, the decoction time is 2h, the stirring speed during extraction is 30r/min, and the extracted basic liquid has good drug effect.

In the step S2, the industrial filter cloth is adopted for filtering, and the industrial filter cloth has good filtering effect.

In the step S1-1, the mechanical extrusion pressure is 10MPa, the extrusion times are 12 times/min, the extrusion time is 5min, the extrusion surface needs to be changed every extrusion, and the gauze is tightened at the same time, so that the liquid oil in the grape seeds is fully leached.

In the step S1-2, the stirring speed is 50r/min, the volatilization time is 3h, and the ethanol is fully volatilized.

Example 2

As shown in fig. 1, a method for preparing an ophthalmic product of oligomeric procyanidin comprises the following steps:

s1: oligomeric proanthocyanidin extraction

S1-1: wrapping grape seeds with gauze, mechanically extruding the grape seeds through the gauze, collecting grape seed oil exuded from the gauze for later use after extrusion is finished, adding grape seed residues and 70% ethanol solution into an extractor according to the mass ratio of 1:2 for extraction, and filtering after extraction is finished to obtain grape seed extracting solution;

s1-2: heating the grape seed extracting solution obtained in the step S1-1 at 63 ℃, stirring to volatilize the ethanol solution to obtain a solution A, and mixing and stirring the solution A and grape seed oil to obtain a solution B;

s1-3: subjecting the mixed solution B to supercritical CO₂Extracting, and separating procyanidine oligomer with the purity of 96.5% by a separator after extraction;

s2: preparing base liquid

Taking the dried medicines in parts by weight: 4 parts of ginseng, 7 parts of wild chrysanthemum, 6 parts of sowthistle tasselflower herb, 4 parts of mint, 5 parts of witch hazel and 4 parts of sea-core kernel are mixed and then put into a crusher for crushing to obtain mixed powder, the mixed powder is poured into a boiler for water extraction, the mass ratio of the mixed powder to water is 1:50, the extraction temperature is 110 ℃, the extraction time is 2.5 hours with stirring, the mixture is filtered after the stirring is finished to obtain filtrate, and then the filtrate is naturally cooled to 10 ℃ to obtain base liquid;

s3: preparing eye liquid

And (4) dripping the procyanidin oligomer obtained in the step (S1), vitamin E and vitamin A into the base solution, and stirring at a stirring speed of 80r/min for 8min to obtain the ophthalmic solution, wherein the mass percent of the procyanidin oligomer in the ophthalmic solution is 1.3%, the mass percent of the vitamin E in the ophthalmic solution is 1.4%, the mass percent of the vitamin A in the ophthalmic solution is 1.0%, and the balance is the base solution.

In the step S1-1, the rotation speed of the extractor is 1800r/min, the extraction time is 4min, the extraction speed is high, and the extraction effect is good.

Supercritical CO₂Extraction of CO₂The flow is 9L/h, the temperature is 60 ℃, the extraction pressure is 30MPa, the pressure in the first-stage separation kettle is 9MPa, the pressure in the second-stage separation kettle is 0.2MPa, the extraction time is 2h, and the procyanidine extraction efficiency is high under the above parameters.

The particle size of the crushed mixed powder in the step S2 is 100-110 μm, so that the medicinal components can be effectively leached.

In the step S2, the decoction time is 2.5h, the stirring speed during extraction is 40r/min, and the extracted basic liquid has good drug effect.

In the step S2, the industrial filter cloth is adopted for filtering, and the industrial filter cloth has good filtering effect.

In the step S1-1, the mechanical extrusion pressure is 15MPa, the extrusion times are 12 times/min, the extrusion time is 6min, the extrusion surface needs to be changed every extrusion, and the gauze is tightened at the same time, so that the liquid oil in the grape seeds is fully leached.

In the step S1-2, the stirring speed is 60r/min, the volatilization time is 3.5h, and the ethanol is fully volatilized.

Example 3

As shown in fig. 1, a method for preparing an ophthalmic product of oligomeric procyanidin comprises the following steps:

s1: oligomeric proanthocyanidin extraction

S1-1: wrapping grape seeds with gauze, mechanically extruding the grape seeds through the gauze, collecting grape seed oil exuded from the gauze for later use after extrusion is finished, adding grape seed residues and 70% ethanol solution into an extractor according to the mass ratio of 1:2 for extraction, and filtering after extraction is finished to obtain grape seed extracting solution;

s1-2: heating the grape seed extracting solution obtained in the step S1-1 at 65 ℃, stirring to volatilize the ethanol solution to obtain a solution A, and mixing and stirring the solution A and grape seed oil to obtain a solution B;

s1-3: subjecting the mixed solution B to supercritical CO₂Extracting, and separating procyanidine oligomer with the purity of 96.5% by a separator after extraction;

s2: preparing base liquid

Taking the dried medicines in parts by weight: 4 parts of ginseng, 8 parts of wild chrysanthemum flower, 6 parts of sowthistle tasselflower herb, 5 parts of mint, 5 parts of witch hazel and 5 parts of sea-core kernel are mixed and then put into a crusher for crushing to obtain mixed powder, the mixed powder is poured into a boiler for water extraction, the mass ratio of the mixed powder to water is 1:50, the extraction temperature is 120 ℃, the extraction time is 3 hours with stirring, after the stirring is finished, the filtrate is obtained by filtering, and then the filtrate is naturally cooled to 15 ℃ to obtain a base solution;

s3: preparing eye liquid

Dripping the procyanidin oligomer obtained in the step S1, vitamin E and vitamin A into the base solution, and stirring at a stirring speed of 80r/min for 5-10min to obtain the ophthalmic solution, wherein the mass percent of the procyanidin oligomer in the ophthalmic solution is 1.4%, the mass percent of the vitamin E in the ophthalmic solution is 1.5%, the mass percent of the vitamin A in the ophthalmic solution is 1.1%, and the balance is the base solution.

In step S1-1, the rotation speed of the extractor is 3000r/min, the extraction time is 6min, the extraction speed is high, and the extraction effect is good.

Supercritical CO₂Extraction of CO₂The flow is 10L/h, the temperature is 70 ℃, the extraction pressure is 40MPa, the pressure in the first-stage separation kettle is 10MPa, the pressure in the second-stage separation kettle is 0.2MPa, the extraction time is 2h, and the procyanidine extraction efficiency is high under the above parameters.

The particle size of the crushed mixed powder in the step S2 is 110-120 μm, so that the medicine components can be effectively leached.

In the step S2, the decoction time is 3h, the stirring speed during extraction is 50r/min, and the extracted basic liquid has good drug effect.

In the step S2, the industrial filter cloth is adopted for filtering, and the industrial filter cloth has good filtering effect.

In the step S1-1, the mechanical extrusion pressure is 20MPa, the extrusion times are 12 times/min, the extrusion time is 6min, the extrusion surface needs to be changed every extrusion, and the gauze is tightened at the same time, so that the liquid oil in the grape seeds is fully leached.

In the step S1-2, the stirring speed is 70r/min, the volatilization time is 4h, and the ethanol is fully volatilized.

Example 4

As shown in fig. 1, a method for preparing an ophthalmic product of oligomeric procyanidin comprises the following steps:

s1: oligomeric proanthocyanidin extraction

S1-1: wrapping grape seeds with gauze, mechanically extruding the grape seeds through the gauze, collecting grape seed oil exuded from the gauze for later use after extrusion is finished, adding grape seed residues and 70% ethanol solution into an extractor according to the mass ratio of 1:2 for extraction, and filtering after extraction is finished to obtain grape seed extracting solution;

s1-2: heating the grape seed extracting solution obtained in the step S1-1 at 65 ℃, stirring to volatilize the ethanol solution to obtain a solution A, and mixing and stirring the solution A and grape seed oil to obtain a solution B;

s1-3: subjecting the mixed solution B to supercritical CO₂Extracting, and separating procyanidine oligomer with the purity of 96.5% by a separator after extraction;

s2: preparing base liquid

Taking the dried medicines in parts by weight: 4 parts of ginseng, 8 parts of wild chrysanthemum flower, 6 parts of sowthistle tasselflower herb, 5 parts of mint, 5 parts of witch hazel and 5 parts of sea-core kernel are mixed and then put into a crusher for crushing to obtain mixed powder, the mixed powder is poured into a boiler for water extraction, the mass ratio of the mixed powder to water is 1:50, the extraction temperature is 120 ℃, the extraction time is 3 hours with stirring, after the stirring is finished, the filtrate is obtained by filtering, and then the filtrate is naturally cooled to 15 ℃ to obtain a base solution;

s3: preparing eye liquid

Dripping the procyanidin oligomer obtained in the step S1, vitamin E and vitamin A into the base solution, and stirring at a stirring speed of 80r/min for 5-10min to obtain the ophthalmic solution, wherein the mass percent of the procyanidin oligomer in the ophthalmic solution is 1.4%, the mass percent of the vitamin E in the ophthalmic solution is 1.5%, the mass percent of the vitamin A in the ophthalmic solution is 1.1%, and the balance is the base solution.

In step S1-1, the rotation speed of the extractor is 3000r/min, the extraction time is 6min, the extraction speed is high, and the extraction effect is good.

Supercritical CO₂The extraction is carried out, and the extraction,CO₂the flow is 10L/h, the temperature is 70 ℃, the extraction pressure is 40MPa, the pressure in the first-stage separation kettle is 10MPa, the pressure in the second-stage separation kettle is 0.2MPa, the extraction time is 2h, and the procyanidine extraction efficiency is high under the above parameters.

The particle size of the crushed mixed powder in the step S2 is 110-120 μm, so that the medicine components can be effectively leached.

In the step S2, the decoction time is 3h, the stirring speed during extraction is 50r/min, and the extracted basic liquid has good drug effect.

In the step S2, the industrial filter cloth is adopted for filtering, and the industrial filter cloth has good filtering effect.

In the step S1-1, the mechanical extrusion pressure is 20MPa, the extrusion times are 12 times/min, the extrusion time is 6min, the extrusion surface needs to be changed every extrusion, and the gauze is tightened at the same time, so that the liquid oil in the grape seeds is fully leached.

In the step S1-2, the stirring speed is 70r/min, the volatilization time is 4h, and the ethanol is fully volatilized.

And (4) taking the eye drop obtained in the step (S3) as an eye drop to be dropped into eyes for relieving eye fatigue and eye dryness.

The ophthalmic solution in step S3 is used for wet dressing of skin around the eyes for moisturizing the skin around the eyes and delaying aging of the skin around the eyes.

Example 5

The oligomeric anthocyanin prepared in example 4 is prepared into eye gel, sprayed, atomized, applied by eye wet house mirror or applied by fumigation of physical therapy mirror. Ophthalmic solutions and fumigation are preferred.

Example 4 two applications of the ophthalmic solution prepared according to the present invention were added to examples 1-3, examples 1-4, the extractor used in step S1-2 was an extractor manufactured by Fei jumping brand, model number FY-SXT-06, and the supercritical CO used in step S1-3₂The extraction equipment is an HA100-50-0.5 type supercritical extraction device produced by Jiangsu Hua' an scientific research limited company, and the separator is a separator provided by the equipment.

And (3) detecting cytotoxicity: the ophthalmic solution prepared by the invention is concentrated into ophthalmic solution with the concentration of 10mg/ML, 50mg/ML and 100mg/ML for toxicity detection.

Detection of the cells used: the results of toxicity test on corneal epithelium and conjunctival epithelium are shown in Table 1

Table 1: results of toxicity test

And (3) oxidation resistance detection:

the results of antioxidant test of arm cells, corneal cells and eyelid cells using the ophthalmic solutions prepared in examples 1 to 3 are shown in fig. 2, in which one test corresponds to example 1, two tests corresponds to example 2, and three tests correspond to example 3.