阅读说明：本技术 Cd3抗原结合片段及其应用 (CD3 antigen binding fragment and application thereof ) 是由 张敬 方丽娟 严永祥 曾亮 周鹏飞 于 2019-02-22 设计创作，主要内容包括：本申请提供了人源化和多功能CD3抗体,其中包括的CD3的重链可变区包含SEQ ID NOs:1-3中任一条的氨基酸序列或其变体序列,轻链可变区包含SEQ ID NOs:26-28中任一条的氨基酸序列或其变体序列。本申请还提供了一种多功能抗体,该抗体包括(a)轻链-重链对,该轻链-重链对针对肿瘤细胞或微生物具有特异性；和(b)融合肽,该融合肽包括单链可变片段和具有CH2结构域和/或CH3结构域的Fc片段,其中该融合肽针对免疫细胞具有特异性。本申请提供的抗体具有改善的生物学活性、热稳定性和/或耐酸性。(The present application provides humanized and multifunctional CD3 antibodies comprising a CD3 heavy chain variable region comprising the amino acid sequence of any one of SEQ ID NOs:1-3 or a variant sequence thereof and a light chain variable region comprising the amino acid sequence of any one of SEQ ID NOs:26-28 or a variant sequence thereof. The present application also provides a multifunctional antibody comprising (a) a light-heavy chain pair specific for a tumor cell or microorganism; and (b) a fusion peptide comprising a single chain variable fragment and an Fc fragment having a CH2 domain and/or a CH3 domain, wherein the fusion peptide is specific for an immune cell. The antibodies provided herein have improved biological activity, thermostability, and/or acid resistance.)