阅读说明：本技术 一种动物胰酶的提取方法及其在纺织品加工上的应用工艺 (Extraction method of animal pancreatin and application process of animal pancreatin in textile processing ) 是由 钟炽均 于 2019-10-28 设计创作，主要内容包括：本发明公开了一种动物胰酶的提取方法及其在纺织品加工上的应用工艺,其特征在于：该动物胰酶的提取方法能直接获得高纯度的、适用纺织品加工要求的动物胰酶产品。本发明所提取的动物胰酶,应用到纺织品加工工艺中,可替代传统纺织加工工艺中所使用的大量化学制品,可实现节能、减排的绿色加工工艺。本发明具有极其现实的推广价值。(The invention discloses an extraction method of animal pancreatin and an application process thereof in textile processing, which is characterized in that: the method for extracting the animal pancreatin can directly obtain the animal pancreatin product with high purity and suitable for the textile processing requirement. The animal pancreatin extracted by the invention is applied to the textile processing technology, can replace a large amount of chemicals used in the traditional textile processing technology, and can realize an energy-saving and emission-reducing green processing technology. The invention has practical popularization value.)

1. The extraction method of trypsin is characterized by comprising the following steps:

the method comprises the following steps: roughly preparing animal pancreatin, namely pretreating animal pancreas to remove connective tissues and fat, cutting into pieces and mincing to obtain pancreatic paste; adding the minced pancreas into an extraction tank, adding acetic acid acidified water until the minced pancreas is immersed, stirring and mixing to obtain a minced pancreas-water mixture, and preparing animal pancreatin to obtain a crude sample for later use;

step two: purifying animal pancreatin, namely centrifuging a prepared crude animal pancreatin sample to be layered, performing pressure filtration on the precipitate, and mixing clear liquid obtained by pressure filtration with supernatant liquid obtained by centrifugation to obtain pancreas extracting solution;

step three: concentrating and finishing animal pancreatin, namely adding 0.1-60mol/L ammonium sulfate into the pancreas extract for gradient salting out to obtain pancreatin mixture; and then filter-pressing the pancreatin mixture, taking a filter cake, uniformly adding 0.1-10mol/L calcium chloride solution into the filter cake until the filter cake is completely dissolved, adding a proper amount of sterilized animal fat and protein for activation to obtain pancreatin concentrated solution, namely the finished product of the animal pancreatin for textile processing.

2. The method a of the first step of claim 1, wherein the pancreas is pretreated by: manually cutting the pancreas into pieces after the fat and connective tissue of the pancreas have been stripped; method b may also be: mincing the pancreas blocks into minced pancreas by a meat mincer; method c may also be: immersing the pancreas pieces in an alkaline degreasing agent, and stirring for degreasing; or a combination of two of the three methods.

3. The second step of claim 1, wherein the centrifugation speed of the sample is 100-.

4. In the third step of claim 1, the ammonium sulfate used for salting out animal pancreatin may be potassium sulfate, sodium sulfate, potassium chloride, sodium chloride or other chemical substances having salting out effect on proteins.

5. In the third step of claim 1, the calcium chloride used for preparing the pancreatin concentrated solution can also be a compound of calcium bromide and the like.

6. The third step of claim 1, wherein the sterilized animal fat and protein may be cooked at a temperature of 100 ℃ or higher, or may be treated with microwave or ultraviolet radiation.

7. The invention also provides an application process for preparing the animal pancreatin in textile processing, which is characterized by comprising the following steps:

(1) degreasing silk spinning raw materials:

(2) the wool scouring process for wool comprises the following steps:

(3) cotton fabrics are refined, so that fatty wax of the cotton can be effectively removed;

(4) the polyester is treated, so that the hydrophilicity of the surface of the polyester can be improved.

8. The process (1) for degreasing silk spinning raw materials as recited in claim 7, wherein the silk spinning is immersed in a pretreatment working solution, treated at 75-100 ℃ for 30-60min, and washed with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L。

9. The process (1) of claim 7, which is used for degreasing silk spinning materials, is characterized in that the process can effectively remove impurities, fatliquoring wax and oil stains on silk spinning, and improve the glossiness, drapability and spinnability of the silk spinning.

10. The process (2) for wool scouring and washing according to claim 7, wherein the wool loose fibers are immersed in a pretreatment working solution, treated at 75-100 ℃ for 30-60min, washed with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L。

11. The process (2) of claim 7, which is used in wool scouring and scouring processes, and is characterized in that it can remove impurities, fatty wax and greasy dirt from wool, significantly improve the brightness of wool and make wool have better spinnability.

12. A process (3) for refining cotton fabric according to claim 7, wherein the cotton fabric is immersed in a pretreatment working solution, treated at 75-100 ℃ for 30-60min, and washed with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L。

13. A refining process (3) for cotton fabric according to claim 7, characterized in that it is capable of removing the impurities, fatty wax and greasy dirt attached to cotton fiber, making the whiteness and capillary effect of cotton fiber obvious and making the cotton fiber get better levelling property and dyeing depth.

14. The process (4) of claim 7, wherein the polyester fiber is immersed in the pretreatment working solution, treated at 75-100 ℃ for 30-60min, and washed with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L。

15. The process (4) of claim 7, wherein the polyester fiber is subjected to oil removal and alkali weight reduction, and the process is characterized in that textile oil and oil stains of the polyester fiber can be removed, and partial polyester staple fiber can be catalyzed and hydrolyzed to achieve the effect of alkali weight reduction of the polyester fiber, so that the fiber smoothness and wear resistance are improved, and the cloth cover quality is improved.

Technical Field

The invention relates to the field of fabric dyeing and finishing auxiliaries, in particular to an extraction method of animal pancreatin and an application process of the animal pancreatin in textile processing.

Background

Animal pancreatin is a digestive enzyme, is secreted by the pancreas of animals, enters the small intestine through the bile duct, and digests food into nutrient substances which are easily absorbed by the small intestine, and is a necessary process for absorbing protein, fat and grease by animals. Pancreatin is a mixture of various enzymes extracted from animal pancreas, and comprises more than 20 biochemical preparations such as trypsin, pancreatic amylase, pancreatic lipase, chymotrypsin and the like. Compared with enzymes extracted from other plants and microorganisms by fermentation, the pancreatin has the characteristics of mild action conditions, low temperature resistance and the like, and is a green enzyme preparation. At present, a large amount of chemicals such as caustic soda, salt and the like are used in the textile processing technology, biodegradation is low, and great pressure is caused on sewage treatment. If the animal pancreatin can replace partial chemicals and is applied to textile processing, the green processing technology of energy conservation and emission reduction can be realized. However, at present, related research in the field of China is rarely reported, so that the method has extremely practical popularization value for the application of animal pancreatin in textile processing.

Pork in China is consumed in a large country and is also the country with the largest live pig feeding amount, 73510 thousands of live pigs are sold in the country in 2014, the total meat yield is 65 percent and is 4 times more than the sum of the cattle and sheep yields, the annual average live pig feeding amount accounts for more than 50 percent of the world total amount, the annual pancreatic output is more than 10 ten thousand tons, and animal pancreatic resources are extremely rich. Therefore, pigs provide the main source for the industrialization of our pancreatin. The extraction of animal pancreas can also be applied to other aspects, for example, the animal pancreas does not have foreign protein reaction to human body in medical application and has certain compatibility, so the pancreatin extracted from animals can also be used as a digestion aid for clinical application and the like, but the medical requirement is high, and the quantitative production of the animal pancreatin is difficult to realize. And the processing requirements of the textile are not harsh for medical use, and the purity requirement is not too high, so that the successful application of the animal pancreatin in the textile processing is beneficial to realizing the quantitative production of the animal pancreatin.

Disclosure of Invention

The invention aims to provide an extraction method of animal pancreatin and an application process thereof in textile processing.

The extraction method of the animal pancreatin provided by the invention is realized by the following steps:

1. the extraction method of trypsin is characterized by comprising the following steps:

the method comprises the following steps: the preparation method of animal pancreatin comprises pretreating animal pancreas to remove connective tissue and fat, cutting into pieces, and mincing to obtain pancreatic chyle. Adding the minced pancreas into an extraction tank, adding acetic acid acidified water until the minced pancreas is immersed, stirring and mixing to obtain a minced pancreas-water mixture, and preparing animal pancreatin to obtain a crude sample for later use;

step two: purifying animal pancreatin, namely centrifuging a prepared crude animal pancreatin sample to be layered, performing pressure filtration on the precipitate, and mixing clear liquid obtained by pressure filtration with supernatant liquid obtained by centrifugation to obtain pancreas extracting solution;

step three: concentrating and finishing animal pancreatin, namely adding 0.1-60mol/L ammonium sulfate into the pancreas extract for gradient salting out to obtain pancreatin mixture; and then filter-pressing the pancreatin mixture, taking a filter cake, uniformly adding 0.1-10mol/L calcium chloride solution into the filter cake until the filter cake is completely dissolved, adding a proper amount of sterilized animal fat and protein for activation to obtain pancreatin concentrated solution, namely the finished product of the animal pancreatin for textile processing.

Preferably, the pretreatment of the pancreas in the first step may be a method a: manually cutting the pancreas into pieces after the fat and connective tissue of the pancreas have been stripped; method b may also be: mincing the pancreas blocks into minced pancreas by a meat mincer; method c may also be: immersing the pancreas pieces in an alkaline degreasing agent, and stirring for degreasing; or a combination of two of the three methods.

Preferably, the centrifugation rate for the sample in step two is 100-5000R/min.

Preferably, the ammonium sulfate used for salting out animal pancreatin in step three may be potassium sulfate, or other chemical substances having a salting out effect on proteins, such as sodium sulfate, potassium chloride, or sodium chloride.

Preferably, the calcium chloride used in the preparation of the pancreatin concentrated solution in step three may be a calcium bromide or other similar compound.

Preferably, the animal fat and protein sterilized in step three may be animal fat and protein cooked at a high temperature of 100 ℃ or higher, or animal fat and protein treated with microwave or ultraviolet wave.

2. The invention also provides an application process for preparing the animal pancreatin in textile processing, which comprises the following steps:

(1) for degreasing silk spinning raw materials

Soaking the silk fabric into a pretreatment working solution, treating at 75-100 ℃ for 30-60min, and washing with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L

The process is characterized in that the process can effectively remove impurities, lipo-wax and oil stains attached to silk spinning, and improve the glossiness, drapability and spinnability of the silk spinning;

(2) wool washing process for wool

Immersing the wool loose fibers into pretreatment working solution, treating at 75-100 ℃ for 30-60min, and washing with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L

The process is characterized in that the method can remove the epibiotic impurities, fatty wax and oil stains of the wool, can obviously improve the brightness of the wool and ensure that the wool obtains better spinnability;

(3) refining cotton fabric

Immersing the cotton grey cloth into a pretreatment working solution, treating at 75-100 ℃ for 30-60min, washing with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L

The process is characterized in that the method can remove the attached impurities, fat wax and oil stains of the cotton fiber, can obviously improve the whiteness and capillary effect of the cotton fiber and ensure that the cotton fiber obtains better level-dyeing property and dyeing depth;

(4) carrying out oil removal and alkali reduction treatment on terylene

Immersing the polyester fiber into a pretreatment working solution, treating at 75-100 ℃ for 30-60min, and washing with hot water and cold water at 60-80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 0.1-5.0g/L

0.1-5.0g/L of alkaline agent

H2O2 0.1-3.0g/L

The process is characterized in that textile oil and oil stains of the polyester fiber can be removed, partial polyester staple fiber can be catalyzed and hydrolyzed, the effect of polyester alkali decrement is achieved, the fiber finish degree and the wear resistance are improved, and the cloth cover quality is improved.

The method for extracting the animal pancreatin has the following advantages:

firstly, the extraction method of the animal pancreatin can directly obtain high-purity animal pancreatin products which are suitable for the textile processing requirements;

secondly, the operation is simple, the extraction yield is high, the production cost is low, and the mass production popularization is facilitated;

the animal pancreatin extracted by the invention is applied to a textile processing technology, can replace a large amount of chemicals used in the traditional textile processing technology, and can realize an energy-saving and emission-reducing green processing technology. The animal pancreatin can be biodegraded, so that the pressure of sewage treatment is greatly reduced. Therefore, the invention has practical popularization value.

Detailed Description

The present invention will be further explained with reference to the following specific examples, but the present invention is not limited to the scope of the examples:

example 1

1. The extraction method of trypsin is characterized by comprising the following steps:

the method comprises the following steps: the animal pancreatin is prepared by pretreating animal pancreas to remove connective tissue and fat, and manually cutting into pieces to obtain pancreatin. Adding the minced pancreas into an extraction tank, adding acetic acid acidified water until the minced pancreas is immersed, stirring and mixing to obtain a minced pancreas-water mixture, and preparing animal pancreatin to obtain a crude sample for later use;

step two: purifying animal pancreatin, namely centrifuging a prepared crude animal pancreatin sample to be layered, performing pressure filtration on the precipitate, and mixing clear liquid obtained by pressure filtration with supernatant liquid obtained by centrifugation to obtain pancreas extracting solution;

step three: concentrating and finishing animal pancreatin, namely adding 20mol/L ammonium sulfate into the pancreas extract for gradient salting out to obtain a pancreatin mixture; then filter-pressing the pancreatin mixture, taking a filter cake, uniformly adding 2mol/L calcium chloride solution into the filter cake until the filter cake is completely dissolved, adding a proper amount of animal fat and protein which are sterilized at high temperature for activation to obtain pancreatin concentrated solution, namely an animal pancreatin finished product for textile processing;

2. the invention also provides an application process for preparing the animal pancreatin in textile processing, which comprises the following steps:

(1) for degreasing silk spinning raw materials

Soaking the silk fabric into a pretreatment working solution, treating at 75-100 ℃ for 60min, and washing with hot water and cold water at 80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 2.0g/L

0.5g/L alkaline agent

H2O2 1.0g/L

The process can effectively remove impurities, lipo-wax and oil stains attached to silk spinning, and improve the glossiness, drapability and spinnability of the silk spinning;

(2) wool washing process for wool

Immersing wool loose fibers into pretreatment working solution, treating at 90 ℃ for 60min, washing with hot water and cold water at 80 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 2.0g/L

3.0g/L alkaline agent

H2O2 1.0g/L

The process can remove the epibiotic impurities, fatty wax and oil stains of the wool, can obviously improve the brightness of the wool and ensure that the wool obtains better spinnability;

(3) the cotton fabric is refined, and the fat wax of the cotton can be effectively removed

Soaking cotton grey cloth in pretreatment working solution, treating at 90 deg.C for 60min, washing with hot water and cold water at 80 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

Alkaline agent 5.0g/L

H2O2 3.0g/L

The process can remove the attached impurities, fat wax and oil stains of the cotton fiber, can obviously show the whiteness and capillary effect of the cotton fiber, and ensures that the cotton fiber obtains better level-dyeing property and dyeing depth;

(4) carrying out oil removal and alkali reduction treatment on terylene

Soaking polyester fiber in pretreatment working solution, treating at 90 deg.C for 60min, washing with hot water and cold water at 80 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

3.0g/L alkaline agent

H2O2 1.0g/L

The process can remove spinning oil and oil stains of the polyester fiber, can also catalyze and hydrolyze part of polyester staple fibers, plays a role in polyester alkali decrement, improves the fiber finish degree and wear resistance, and improves the cloth cover quality;

example 2

1. The extraction method of trypsin is characterized by comprising the following steps:

the method comprises the following steps: the animal pancreatin is prepared by pretreating animal pancreas to remove connective tissue and fat, cutting into pieces and mincing with meat mincer to obtain pancreas paste. Adding the minced pancreas into an extraction tank, adding acetic acid acidified water until the minced pancreas is immersed, stirring and mixing to obtain a minced pancreas-water mixture, and preparing animal pancreatin to obtain a crude sample for later use;

step two: purifying animal pancreatin, namely centrifuging a prepared crude animal pancreatin sample to be layered, performing pressure filtration on the precipitate, and mixing clear liquid obtained by pressure filtration with supernatant liquid obtained by centrifugation to obtain pancreas extracting solution;

step three: concentrating and finishing animal pancreatin, namely adding 60mol/L ammonium sulfate into the pancreas extract for gradient salting out to obtain a pancreatin mixture; then filter-pressing the pancreatin mixture, taking a filter cake, uniformly adding 8mol/L calcium chloride solution into the filter cake until the filter cake is completely dissolved, adding a proper amount of animal fat and protein which are subjected to ultraviolet wave sterilization treatment for activation to obtain pancreatin concentrated solution, namely an animal pancreatin finished product for textile processing;

2. the invention also provides an application process for preparing the animal pancreatin in textile processing, which comprises the following steps:

(1) for degreasing silk spinning raw materials

Soaking silk in pretreatment working solution, treating at 85 deg.C for 60min, washing with hot water and cold water at 85 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

2g/L alkaline agent

H2O2 2.0g/L

The process can effectively remove impurities, lipo-wax and oil stains attached to silk spinning, and improve the glossiness, drapability and spinnability of the silk spinning;

(2) wool washing process for wool

Immersing the wool loose fibers into pretreatment working solution, treating at 85 ℃ for 60min, washing with hot water and cold water at 85 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

3.0g/L alkaline agent

H2O2 3.0g/L

The process can remove the epibiotic impurities, fatty wax and oil stains of the wool, can obviously improve the brightness of the wool and ensure that the wool obtains better spinnability;

(3) the cotton fabric is refined, and the fat wax of the cotton can be effectively removed

Soaking cotton grey cloth in pretreatment working solution, treating at 85 deg.C for 60min, washing with hot water and cold water at 85 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

Alkaline agent 5.0g/L

H2O2 3.0g/L

The process can remove the attached impurities, fat wax and oil stains of the cotton fiber, can obviously show the whiteness and capillary effect of the cotton fiber, and ensures that the cotton fiber obtains better level-dyeing property and dyeing depth;

(4) carrying out oil removal and alkali reduction treatment on terylene

Soaking polyester fiber in pretreatment working solution, treating at 85 deg.C for 60min, washing with hot water and cold water at 85 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 4.0g/L

3.0g/L alkaline agent

H2O2 3.0g/L

The process can remove spinning oil and oil stains of the polyester fiber, can also catalyze and hydrolyze part of polyester staple fibers, plays a role in polyester alkali decrement, improves the fiber finish degree and wear resistance, and improves the cloth cover quality;

example 3

1. The extraction method of trypsin is characterized by comprising the following steps:

the method comprises the following steps: the animal pancreatin is prepared by pretreating animal pancreas to remove connective tissue and fat, cutting into pieces and mincing with meat mincer to obtain pancreas paste. Adding the minced pancreas into an extraction tank, adding acetic acid acidified water until the minced pancreas is immersed, stirring and mixing to obtain a minced pancreas-water mixture, and preparing animal pancreatin to obtain a crude sample for later use;

step two: purifying animal pancreatin, namely centrifuging a prepared crude animal pancreatin sample to be layered, performing pressure filtration on the precipitate, and mixing clear liquid obtained by pressure filtration with supernatant liquid obtained by centrifugation to obtain pancreas extracting solution;

step three: concentrating and finishing animal pancreatin, namely adding 40mol/L ammonium sulfate into the pancreas extract for gradient salting out to obtain a pancreatin mixture; then filter-pressing the pancreatin mixture, taking a filter cake, uniformly adding 40mol/L calcium bromide solution into the filter cake until the filter cake is completely dissolved, adding a proper amount of animal fat and protein which are subjected to microwave sterilization treatment for activation to obtain pancreatin concentrated solution, namely an animal pancreatin finished product for textile processing;

2. the invention also provides an application process for preparing the animal pancreatin in textile processing, which comprises the following steps:

(1) for degreasing silk spinning raw materials

Soaking silk in pretreatment working solution, treating at 95 deg.C for 60min, washing with hot water and cold water at 90 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

Alkaline agent 2.0g/L

H2O2 2.0g/L

The process can effectively remove impurities, lipo-wax and oil stains attached to silk spinning, and improve the glossiness, drapability and spinnability of the silk spinning;

(2) wool washing process for wool

Immersing wool loose fibers into pretreatment working solution, treating at 95 ℃ for 60min, washing with hot water and cold water at 90 ℃; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 3.0g/L

Alkaline agent 2.0g/L

H2O2 1.0g/L

The process can remove the epibiotic impurities, fatty wax and oil stains of the wool, can obviously improve the brightness of the wool and ensure that the wool obtains better spinnability;

(3) the cotton fabric is refined, and the fat wax of the cotton can be effectively removed

Soaking cotton grey cloth in pretreatment working solution, treating at 95 deg.C for 60min, washing with hot water and cold water at 90 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 5.0g/L

3.0g/L alkaline agent

H2O2 2.0g/L

The process can remove the attached impurities, fat wax and oil stains of the cotton fiber, can obviously show the whiteness and capillary effect of the cotton fiber, and ensures that the cotton fiber obtains better level-dyeing property and dyeing depth;

(4) carrying out oil removal and alkali reduction treatment on terylene

Soaking polyester fiber in pretreatment working solution, treating at 95 deg.C for 60min, washing with hot water and cold water at 90 deg.C; the formula of the pretreatment finishing liquid is as follows:

animal pancreatin 4.0g/L

Alkaline agent 4.0g/L

H2O2 2.0g/L

The process can remove spinning oil and oil stains of the polyester fiber, can also catalyze and hydrolyze part of polyester staple fibers, plays a role in polyester alkali decrement, improves the fiber finish degree and wear resistance, and improves the cloth cover quality.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included therein.