阅读说明：本技术 一种欧李原花青素、其制备方法、其应用和药物 (Prunus humilis procyanidin, preparation method, application and medicine thereof ) 是由 李卫东 崔清宇 李宗朔 于 2020-07-15 设计创作，主要内容包括：本发明适用于医药技术领域,提供了一种欧李原花青素、其制备方法、其应用和药物,该欧李原花青素的制备方法包括以下步骤：取欧李,并进行去核处理,得到去核欧李；将去核欧李与乙醇水溶液置于30~50℃的温度下进行超声波提取,得到提取液；将提取液进行抽滤后,再进行真空浓缩和冻干处理,得到所述欧李原花青素。本发明通过将欧李中的活性成分提取制备成冻干粉,可以实现在维持其生物活性的同时,还便于储存运输,其对于欧李产业的发展以及满足人们日益增长的大健康需求具有重要意义。另外,本发明制得的欧李原花青素具有预防骨质疏松,维持骨骼结构,保护骨小梁微结构的完整,改善绝经所致的雌激素紊乱及调控机体肠道菌群代谢物等功效。(The invention is applicable to the technical field of medicines, and provides prunus humilis procyanidin, a preparation method, application and a medicine thereof, wherein the preparation method of prunus humilis procyanidin comprises the following steps: taking cerasus humilis, and performing denucleation treatment to obtain denucleated cerasus humilis; placing the denucleated cerasus humilis and an ethanol aqueous solution at the temperature of 30-50 ℃ for ultrasonic extraction to obtain an extracting solution; and carrying out suction filtration on the extracting solution, and then carrying out vacuum concentration and freeze-drying treatment to obtain the prunus humilis procyanidin. According to the invention, the active ingredients in the cerasus humilis are extracted and prepared into the lyophilized powder, so that the biological activity of the cerasus humilis can be maintained, and the cerasus humilis lyophilized powder is convenient to store and transport, and has important significance for the development of the cerasus humilis industry and the meeting of the ever-growing health requirements of people. In addition, the prunus humilis procyanidin prepared by the invention has the effects of preventing osteoporosis, maintaining the bone structure, protecting the integrity of the trabecular bone microstructure, improving estrogen disorder caused by menopause, regulating and controlling intestinal flora metabolites of organisms and the like.)

1. The preparation method of prunus humilis procyanidin is characterized by comprising the following steps:

taking cerasus humilis, and performing denucleation treatment to obtain denucleated cerasus humilis;

placing the denucleated cerasus humilis and an ethanol aqueous solution at the temperature of 30-50 ℃ for ultrasonic extraction to obtain an extracting solution;

and carrying out suction filtration on the extracting solution, and then carrying out vacuum concentration and freeze-drying treatment to obtain the prunus humilis procyanidin.

2. The method of preparing prunus humilis procyanidin as claimed in claim 1, wherein the volume concentration of the ethanol aqueous solution is 60-80%.

3. The method for preparing prunus humilis procyanidin as claimed in claim 2, wherein the mass-volume ratio of the denucleated prunus humilis to the ethanol aqueous solution is 1 (3-7) in g/mL.

4. The method for preparing procyanidine from cerasus humilis Bunge as claimed in claim 1, wherein in the step, the ultrasonic power for ultrasonic extraction is 400-800W, and the ultrasonic frequency is 30-50 KHz.

5. The method for preparing procyanidins from Prunus humilis Bunge as claimed in claim 1, wherein the vacuum concentration temperature is 35-55 ℃.

6. The method for preparing procyanidins from prunus humilis as claimed in claim 1, wherein the step of performing suction filtration on the extract specifically comprises:

and carrying out suction filtration on the extracting solution by using 400-600 mesh nylon filter cloth.

7. A prunus humilis procyanidin prepared by the preparation method of any one of claims 1 to 6.

8. Use of prunus humilis procyanidin as defined in claim 7 in the preparation of a medicament for the treatment and/or prevention of osteoporosis.

9. Use of prunus humilis procyanidins as defined in claim 7 in the preparation of a medicament for ameliorating estrogen disorders and/or modulating metabolites of the intestinal flora of the body.

10. A medicament for the treatment and/or prevention of osteoporosis comprising a pharmaceutical carrier, further comprising prunus humilis procyanidin as claimed in claim 7.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to prunus humilis procyanidin, a preparation method, application and a medicine thereof.

Background

Osteoporosis is the most common bone disease, a systemic bone disease characterized by low bone mass, damaged microstructure of bone tissue, increased bone fragility, and susceptibility to fracture, and is also called "silent disease" because it has no specific clinical symptoms at its early stage. With the coming of the aging society of the population, the incidence rate of osteoporosis is increasing year by year and is also more and more emphasized.

At present, the main method for treating osteoporosis is mainly drug therapy. Anti-osteoporosis drugs are based primarily on two concepts: inhibiting bone resorption or inducing bone formation. Commonly used bone resorption inhibitors are mainly bisphosphonates, calcitonin, estrogens and selective estrogen receptor modulators, which inhibit bone resorption mainly by inhibiting osteoclast function or activity. The bone formation promoting medicine with a definite mechanism mainly comprises parathyroid hormone analogues.

However, gastrointestinal intolerance, poor patient compliance, and safety issues during long-term treatment are controversial as a result of these drugs. Therefore, from the perspective of food-medicine homology or natural medicine, finding a safe and effective method capable of preventing and even treating osteoporosis is the key to solving the current problems.

Disclosure of Invention

The embodiment of the invention aims to provide a preparation method of prunus humilis procyanidin, aiming at solving the problems in the background art.

The embodiment of the invention is realized in such a way that the preparation method of the prunus humilis procyanidin comprises the following steps:

taking cerasus humilis, and performing denucleation treatment to obtain denucleated cerasus humilis;

placing the denucleated cerasus humilis and an ethanol aqueous solution at the temperature of 30-50 ℃ for ultrasonic extraction to obtain an extracting solution;

and carrying out suction filtration on the extracting solution, and then carrying out vacuum concentration and freeze-drying treatment to obtain the prunus humilis procyanidin.

As a preferable scheme of the embodiment of the invention, the volume concentration of the ethanol aqueous solution is 60-80%.

As another preferable scheme of the embodiment of the invention, the mass volume ratio of the denucleated cerasus humilis to the ethanol water solution is 1 (3-7) in g/mL.

As another preferable scheme of the embodiment of the invention, in the step, the ultrasonic power of ultrasonic wave extraction is 400-800W, and the ultrasonic frequency is 30-50 KHz.

As another preferable scheme of the embodiment of the invention, in the step, the temperature of vacuum concentration is 35-55 ℃.

As another preferable scheme of the embodiment of the present invention, the step of performing suction filtration on the extract specifically includes:

and carrying out suction filtration on the extracting solution by using 400-600 mesh nylon filter cloth.

Another object of the embodiments of the present invention is to provide prunus humilis procyanidin prepared by the above preparation method.

Another object of the present invention is to provide a use of prunus humilis procyanidins described above in the preparation of a medicament for treating and/or preventing osteoporosis.

Another object of the embodiments of the present invention is to provide an application of prunus humilis procyanidins in preparing a drug for improving estrogen disorders and/or regulating and controlling metabolites of intestinal flora of a body.

Another object of an embodiment of the present invention is to provide a pharmaceutical composition for treating and/or preventing osteoporosis, comprising a pharmaceutical carrier and the above prunus humilis procyanidin.

According to the preparation method of the prunus humilis procyanidin provided by the embodiment of the invention, the active ingredients in the prunus humilis are extracted and prepared into the lyophilized powder, so that the biological activity of the prunus humilis procyanidin can be maintained, and the prunus humilis procyanidin is convenient to store and transport, and has important significance for the development of the prunus humilis industry and the meeting of the increasing health requirements of people. In addition, the prunus humilis procyanidin prepared by the embodiment of the invention has an obvious effect of preventing osteoporosis. Wherein, the prunus humilis is a plant used as both medicine and food, and the prunus humilis procyanidin extract freeze-dried powder is obtained after extraction and preparation, has the effects of preventing osteoporosis, maintaining the skeleton structure, protecting the integrity of the trabecular bone microstructure, improving estrogen disorder caused by menopause, regulating and controlling intestinal flora metabolites (short chain fatty acids) of the organism and the like; meanwhile, the procyanidine from cerasus humilis is safe in foodstuff, has no obvious toxic or side effect, and solves the problem of toxic or side effect caused by the fact that most western medicines are adopted to treat osteoporosis at present.

Drawings

FIG. 1 is a microphysis micro-CT three-dimensional scan of femoral metaphysis of mice of different groups;

FIG. 2 is a graph of H & E staining of femoral metaphysis in different groups of mice;

FIG. 3 is a graph of the metaphyseal TRAP staining of femoral bones of different groups of mice;

FIG. 4 is a graph comparing the estradiol secretion levels in different groups of mice;

FIG. 5 is a graph comparing the levels of PINP in the bodies of mice from different groups;

FIG. 6 is a graph comparing the levels of OPG/sRANKL in different groups of mouse bodies;

FIG. 7 is a graph comparing the acetic acid content in different groups of mouse feces;

FIG. 8 is a graph comparing the propionic acid content in different groups of mouse feces;

FIG. 9 is a graph comparing the isobutyric acid content in different groups of mouse feces;

FIG. 10 is a graph comparing the n-butyric acid content in different groups of mouse feces;

FIG. 11 is a graph comparing the content of isovaleric acid in different groups of mouse feces;

FIG. 12 is a graph comparing the content of n-pentanoic acid in mouse feces of different groups.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.