阅读说明：本技术 木犀草素促进头孢噻呋渗透性的应用 (Application of luteolin in promoting ceftiofur permeability ) 是由 刘明春 王如霞 康芷若 田春莲 张徳显 于 2020-08-21 设计创作，主要内容包括：本发明属于医药技术领域,具体涉及木犀草素促进头孢噻呋渗透性的应用。以4周龄健康雏鸭的一段空肠,建立离体外翻肠囊模型,制备外翻肠囊,检测其肠活性；建立头孢噻呋和木犀草素的HPLC检测方法,通过HPLC法检测木犀草素和头孢噻呋浓度,计算两者的表观渗透系数、吸收率和吸收速率参数,由此验证木犀草素能够促进头孢噻呋在生物体内的渗透性,提高其生物利用度。本发明为深入探究天然药物与化药联合应用时两者之间的相互作用奠定了理论基础。(The invention belongs to the technical field of medicines, and particularly relates to application of luteolin in promoting ceftiofur permeability. Establishing an in-vitro intestine turning capsule model by using a section of jejunum of a healthy duckling at the age of 4 weeks, preparing an intestine turning capsule, and detecting the intestinal activity of the intestine turning capsule; establishing an HPLC detection method for ceftiofur and luteolin, detecting the concentrations of the luteolin and the ceftiofur by an HPLC method, and calculating the apparent permeability coefficient, the absorption rate and the absorption rate parameters of the luteolin and the ceftiofur, thereby verifying that the luteolin can promote the permeability of the ceftiofur in the living body and improving the bioavailability of the ceftiofur. The invention lays a theoretical foundation for deeply exploring the interaction between the natural medicine and the chemical medicine when the natural medicine and the chemical medicine are applied in a combined way.)

1. Application of luteolin in promoting ceftiofur permeability is provided.

2. The use according to claim 1, wherein the luteolin facilitates absorption of ceftiofur in the gut of the avian.

3. The use according to claim 3, wherein the bird is a duck.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to an influence of luteolin on permeability of ceftiofur in duck jejunum.

Background

Ceftiofur has the advantages of strong bactericidal power, wide antibacterial spectrum, small toxic effect, small allergic reaction and the like, and is widely applied to prevention and treatment of bacterial diseases of beef cattle, dairy cows, pigs, sheep, poultry and the like in veterinary clinic. Ceftiofur is absorbed mainly in the intestinal tract after oral administration. Related researches show that the bioavailability of ceftiofur in duck bodies is less than 90%, the ceftiofur belongs to low-permeability medicines, and the low bioavailability of ceftiofur is possibly related to efflux transporters on intestinal mucosa, particularly P-glycoprotein.

Luteolin is a natural compound contained in a plurality of plants, and particularly, a great amount of luteolin is contained in fruits and vegetables which are eaten by people at ordinary times. Because it has pharmacological actions of bacteriostasis, antivirus, immunoregulation, anti-tumor, anti-inflammation, antioxidation, etc., it can be used for preventing and curing diseases. Therefore, people can change the diet to determine the intake of luteolin, so as to reduce the occurrence of diseases. So far, no report about the influence of flavonoid compounds such as luteolin on the absorption of ceftiofur in the duck intestinal tract is available.

Disclosure of Invention

In order to solve the technical problems, the invention provides a new application of luteolin, namely the application of luteolin in promoting ceftiofur permeability.

In order to realize the purpose, the invention adopts the following technical scheme:

application of luteolin in promoting ceftiofur permeability is provided.

In the technical scheme, furthermore, the luteolin is used for promoting the absorption of ceftiofur in the intestinal tracts of the poultry.

In the above technical scheme, further, the poultry is a duck.

The invention has the beneficial effects that: the invention firstly provides that the luteolin is applied to the living body to promote the permeability of ceftiofur in the living body, so that the absorption of ceftiofur is increased, the bioavailability is improved, the drug effect is better exerted, and the theoretical basis is laid for further researching the interaction between the natural medicine and the chemical medicine when the natural medicine and the chemical medicine are applied in a combined way.

Drawings

FIG. 1 is a graph showing the trend of the final volume of serosal fluid with respect to the culture time.

FIG. 2 is a graph comparing the apparent permeability coefficients of ceftiofur of different groups in jejunum of ducks.

FIG. 3 shows the trend of the absorption (%) of ceftiofur in duck jejunum with time for different groups.

FIG. 4 is a graph showing the time-dependent trend of the absorption rate of ceftiofur in different groups in the jejunum of ducks.

Detailed Description

In order to make the objects, features and advantages of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it should be apparent that the embodiments described below are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the following embodiment, a duckling in vitro intestine turning method is adopted for research, an in vitro intestine turning bag model is established by taking a section of jejunum of a healthy duckling at the age of 4 weeks, an intestine turning bag is prepared, and the intestinal activity of the intestine turning bag is detected; establishing an HPLC detection method for ceftiofur and luteolin, detecting the concentrations of the luteolin and the ceftiofur by an HPLC method, and calculating the apparent permeability coefficient, the absorptivity and the absorption rate parameters of the two, thereby verifying that the luteolin can promote the permeability of the ceftiofur in the organism. The method has the advantages of strong controllability of test conditions, short test period, simplicity and feasibility, and good in-vivo correlation of test data.

Ceftiofur hydrochloride used in the following examples, purity 92.1%, was purchased from zilu animal health products, ltd; de-furoyl ceftiofur (DFC) standard, 98.0% pure, available from sigma; luteolin standard with purity of 98% is purchased from Shanghai Purui Biotech limited; dichloroerythrol (DTE) with a purity of 99.0%, Biotechnology engineering (Shanghai) Ltd.