阅读说明：本技术 β-1,3-葡聚糖在制备修复皮肤损伤药物和日化产品中的应用 (Application of beta-1, 3-glucan in preparation of medicines and daily chemical products for repairing skin injuries ) 是由 杜彦军 杜玲玲 于 2020-07-31 设计创作，主要内容包括：本发明公开了β-1,3-葡聚糖在制备修复皮肤损伤药物和日化产品中的应用,本发明的目的在于将β-1,3-葡聚糖引入到修复皮肤损伤的治疗中,公开了β-1,3-葡聚糖的新用途,公开了β-1,3-葡聚糖在制备修复皮肤损伤药物和日化产品中的应用；本发明通过实验证实了β-1,3-葡聚糖具有明显的抗炎、促进组织再生和促进伤口愈合的作用,可以表明β-1,3-葡聚糖对皮肤具有修复作用,β-1,3-葡聚糖可以应用在制备修复皮肤损伤的药物和日化产品中,为同类药物和日化产品的开发奠定了一定的基础。(The invention discloses application of beta-1, 3-glucan in preparing a medicament for repairing skin injury and a daily chemical product, aims to introduce the beta-1, 3-glucan into treatment for repairing skin injury, discloses new application of the beta-1, 3-glucan, and discloses application of the beta-1, 3-glucan in preparing the medicament for repairing skin injury and the daily chemical product; experiments prove that the beta-1, 3-glucan has obvious effects of resisting inflammation, promoting tissue regeneration and promoting wound healing, can show that the beta-1, 3-glucan has a repairing effect on skin, and the beta-1, 3-glucan can be applied to preparation of medicines and daily chemical products for repairing skin injury, thereby laying a certain foundation for development of similar medicines and daily chemical products.)

1. Application of beta-1, 3-glucan in preparing medicines and daily chemical products for repairing skin injury.

2. Application of beta-1, 3-glucan in preparing anti-inflammatory drugs and daily chemical products for skin.

3. Application of beta-1, 3-glucan in preparing medicines and daily chemical products for promoting skin tissue regeneration.

4. Application of beta-1, 3-glucan in preparing medicines and daily chemical products for promoting skin wound healing.

5. Use according to any one of claims 1 to 4, characterized in that: the beta-1, 3-glucan can be used alone or in combination with other drugs.

6. Use according to any one of claims 1 to 4, characterized in that: the preparation of the beta-1, 3-glucan comprises the following components in parts by weight: 15-25 parts of sodium dihydrogen phosphate, 35-45 parts of potassium nitrate, 3-5 parts of magnesium sulfate, 0.1-0.2 part of calcium chloride, 0.2-0.3 part of ferrous sulfate, 0.04-0.08 part of manganese sulfate, 35-45 parts of rapeseed oil and 620 parts of cane sugar 580-doped materials; water 169900 and 17100 shares;

the preparation also comprises an agrobacterium ZX09 seed solution, and the volume ratio of the agrobacterium ZX09 seed solution to the total volume of the components is 1: 95-105.

7. Use according to claim 6, characterized in that: when the beta-1, 3-glucan is prepared, the weight parts of the components are respectively as follows: 18-22 parts of sodium dihydrogen phosphate, 38-42 parts of potassium nitrate, 3.5-4.5 parts of magnesium sulfate, 0.12-0.18 part of calcium chloride, 0.22-0.28 part of ferrous sulfate, 0.05-0.07 part of manganese sulfate, 38-42 parts of rapeseed oil and 610 parts of sucrose 590-; 17050 parts of 16952 parts of water;

the volume ratio of the agrobacterium ZX09 seed solution to the total volume of the components is 1: 98-102.

8. Use according to claim 7, characterized in that: when the beta-1, 3-glucan is prepared, the weight parts of the components are respectively as follows: 20 parts of sodium dihydrogen phosphate, 40 parts of potassium nitrate, 4 parts of magnesium sulfate, 0.14 part of calcium chloride, 0.25 part of ferrous sulfate, 0.06 part of manganese sulfate, 40 parts of rapeseed oil and 600 parts of sucrose; 17000 parts of water;

the volume ratio of the agrobacterium ZX09 seed solution to the total volume of the components is 1: 100.

9. Use according to claim 8, characterized in that: the agrobacterium ZX09 seed solution is a mixture formed after agrobacterium ZX09 grows in a culture medium, and the culture medium comprises the following components in parts by weight: 210 portions of water 190-plus, 1.5-2.5 portions of peptone, 0.8-1.2 portions of yeast powder and 1.5-2.5 portions of sodium chloride.

10. Use according to claim 9, characterized in that: the preparation of the beta-1, 3-glucan comprises the following steps:

(1) adding part of water, sodium dihydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, rapeseed oil and sucrose into a fermentation tank according to the proportion, dissolving and uniformly stirring to obtain a mixed solution;

(2) adjusting the pH value of the mixed solution obtained in the step (1) to 6.5-7.5, and then carrying out steam sterilization;

(3) cooling the mixed solution subjected to steam sterilization in the step (2) to room temperature;

(4) adding the seed solution of the agrobacterium ZX09 into the mixed solution cooled in the step (3) according to the proportion, adjusting the ventilation flow to 18-22L/min, setting the stirring speed to 250-270rpm and the fermentation temperature to 28-32 ℃, culturing for 58-62h, and obtaining the fermentation liquid after the fermentation is finished;

(5) putting the fermentation liquor obtained in the step (4) into a barrel, adding 95% ethanol to precipitate the fermentation liquor, taking the precipitate, drying by pressure, dissolving the precipitate in the rest water, adding sodium hydroxide and diatomite, uniformly mixing, heating to 88-92 ℃ to obtain a suspension, and repeatedly filtering the suspension by using a plate-and-frame filter press until the solution is clear and transparent; the precipitate and filtrate are retained;

(6) adding 95% ethanol into the filtrate obtained in the step (5) to precipitate the filtrate, and filtering again; keeping the precipitate;

(7) and (4) mixing the precipitates obtained in the step (5) and the step (6), and sequentially performing press drying, drying and crushing to obtain a finished product of the beta-1, 3-glucan.

Technical Field

The invention relates to the technical field of medicines, in particular to application of beta-1, 3-glucan in preparation of medicines and daily chemical products for repairing skin injuries.

Background

Beta-glucan is a macromolecular polysaccharide widely existing in cell walls of microorganisms, plants and seaweeds, a main chain structure is connected by beta-1, 3-glycosidic bonds beta and/or beta-1, 4 glycosidic bonds, and usually contains branched chains connected by beta-1, 6 bonds in different proportions, and main cell walls exist in the form of cell structural components. The glucan containing the beta-1, 3-glycosidic bond has stronger induction and activation effects on a foreign host defense system. In the 40 s of the 20 th century, Piller and Ecker, et al found that an immunostimulating active was present in yeast cells, and until the 60 s of the 20 th century, this active was not confirmed to be β -glucan.

Disclosure of Invention

The invention aims to provide application of beta-1, 3-glucan in preparation of a medicine for repairing skin injury and a daily chemical product. The technical effects that can be produced by the preferred technical scheme in the technical schemes provided by the invention are described in detail in the following.

The invention discloses that the beta-1, 3-glucan has the effect of repairing skin for the first time and can be used for treating skin injury.

The invention particularly discloses application of beta-1, 3-glucan in preparation of a medicine for repairing skin injury and a daily chemical product.

The invention discloses application of beta-1, 3-glucan in preparation of anti-inflammatory drugs for skin and daily chemical products.

The invention discloses application of beta-1, 3-glucan in preparation of a medicament and a daily chemical product for promoting skin tissue regeneration.

The invention discloses application of beta-1, 3-glucan in preparation of medicines and daily chemical products for promoting skin wound healing.

Further, the medicine is an oral medicine or an external medicine; the dosage form of the oral medicine is tablets, capsules or granules; the topical medicine is in the form of powder, lotion, spirit, tincture, ointment, cream, oil, hard preparation or film preparation.

Further, the beta-1, 3-glucan can be used alone or in the form of a pharmaceutical composition comprising a therapeutically effective amount of beta-1, 3-glucan.

Further, the preparation of the beta-1, 3-glucan comprises the following components in parts by weight: 15-25 parts of sodium dihydrogen phosphate, 35-45 parts of potassium nitrate, 3-5 parts of magnesium sulfate, 0.1-0.2 part of calcium chloride, 0.2-0.3 part of ferrous sulfate, 0.04-0.08 part of manganese sulfate, 35-45 parts of rapeseed oil and 620 parts of cane sugar 580-doped materials; water 169900 and 17100 shares;

the preparation also comprises an agrobacterium ZX09 seed solution, and the volume ratio of the agrobacterium ZX09 seed solution to the total volume of the components is 1: 95-105.

Further, when the beta-1, 3-glucan is prepared, the weight parts of the components are respectively as follows: 18-22 parts of sodium dihydrogen phosphate, 38-42 parts of potassium nitrate, 3.5-4.5 parts of magnesium sulfate, 0.12-0.18 part of calcium chloride, 0.22-0.28 part of ferrous sulfate, 0.05-0.07 part of manganese sulfate, 38-42 parts of rapeseed oil and 610 parts of sucrose 590-; 17050 parts of 16952 parts of water;

the volume ratio of the agrobacterium ZX09 seed solution to the total volume of the components is 1: 98-102.

Further, when the beta-1, 3-glucan is prepared, the weight parts of the components are respectively as follows: 20 parts of sodium dihydrogen phosphate, 40 parts of potassium nitrate, 4 parts of magnesium sulfate, 0.14 part of calcium chloride, 0.25 part of ferrous sulfate, 0.06 part of manganese sulfate, 40 parts of rapeseed oil and 600 parts of sucrose; 17000 parts of water;

the volume ratio of the agrobacterium ZX09 seed solution to the total volume of the components is 1: 100.

Further, the agrobacterium ZX09 seed solution is a mixture formed after agrobacterium ZX09 grows in a culture medium, and the culture medium comprises the following components in parts by weight: 210 portions of water 190-plus, 1.5-2.5 portions of peptone, 0.8-1.2 portions of yeast powder and 1.5-2.5 portions of sodium chloride.

Further, when preparing the beta-1, 3-glucan, the method comprises the following steps:

(1) adding part of water, sodium dihydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, rapeseed oil and sucrose into a fermentation tank according to the proportion, dissolving and uniformly stirring to obtain a mixed solution;

(2) adjusting the pH value of the mixed solution obtained in the step (1) to 6.5-7.5, and then carrying out steam sterilization;

(3) cooling the mixed solution subjected to steam sterilization in the step (2) to room temperature;

(4) adding the seed solution of the agrobacterium ZX09 into the mixed solution cooled in the step (3) according to the proportion, adjusting the ventilation flow to 18-22L/min, setting the stirring speed to 250-270rpm and the fermentation temperature to 28-32 ℃, culturing for 58-62h, and obtaining the fermentation liquid after the fermentation is finished;

(5) putting the fermentation liquor obtained in the step (4) into a barrel, adding 95% ethanol to precipitate the fermentation liquor, taking the precipitate, drying by pressure, dissolving the precipitate in the rest water, adding sodium hydroxide and diatomite, uniformly mixing, heating to 88-92 ℃ to obtain a suspension, and repeatedly filtering the suspension by using a plate-and-frame filter press until the solution is clear and transparent; the precipitate and filtrate are retained;

(6) adding 95% ethanol into the filtrate obtained in the step (5) to precipitate the filtrate, and filtering again; keeping the precipitate;

(7) and (4) mixing the precipitates obtained in the step (5) and the step (6), and sequentially performing press drying, drying and crushing to obtain a finished product of the beta-1, 3-glucan.

Further, in the step (2), the steam sterilization is performed for 25-35min at 121 ℃ when preparing the beta-1, 3-glucan.

The structural formula of the beta-1, 3-glucan prepared by the invention is as follows:

the general structural formula is as follows:

poly-3) - β -D-pyran- (l → 3) - [ β -D-pyran- (l → 3) - β -D-pyran-]₃- (l → 3) - α -D-pyran- (l → 3) - α -D-pyran- (l → glucose.

The action mechanism of the beta-1, 3-glucan for repairing the skin injury is as follows:

the beta-1, 3-glucan promotes the healing of skin wounds by chemotactic fibroblasts, promoting epidermal regeneration, promoting angiogenesis, and promoting the expression of cytokines TGF-beta 1 (transforming growth factor-beta 1), FGF-2 (fibroblast growth factor-2), EGF (epidermal growth factor) and a cell cycle regulatory protein Cyclin D1.

Based on the technical scheme, the embodiment of the invention can at least produce the following technical effects:

the invention aims to introduce beta-1, 3-glucan into the treatment of repairing skin injury, discloses a new application of the beta-1, 3-glucan, and discloses an application of the beta-1, 3-glucan in preparing medicines and daily chemical products for repairing skin injury; experiments prove that the beta-1, 3-glucan has obvious effects of resisting inflammation, promoting tissue regeneration and promoting wound healing, can show that the beta-1, 3-glucan has a repairing effect on skin, and the beta-1, 3-glucan can be applied to preparation of medicines and daily chemical products for repairing skin injury, thereby laying a certain foundation for development of similar medicines and daily chemical products.

Drawings

In order to illustrate the embodiments or prior art solutions more clearly, the drawings used in the description of the embodiments or prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present description, and it is obvious for a person skilled in the art that other drawings can be obtained from these drawings without inventive effort.

FIG. 1 is a graphical representation of the anti-inflammatory effect of beta-1, 3-glucan on zebrafish inflammation;

FIG. 2 shows the anti-inflammatory effect of beta-1, 3-glucan on zebrafish inflammation (neutrophil count);

FIG. 3 is a graph showing the inflammation resolution rate of beta-1, 3-glucan against inflammation in zebrafish;

FIG. 4 is a typical graph of regeneration (arrow marks) of zebrafish tissue after β -1, 3-glucan treatment;

FIG. 5 shows the regeneration length (in pixels) of zebrafish tissue after beta-1, 3-glucan treatment;

FIG. 6 shows the tissue regeneration promoting effect of zebra fish treated with beta-1, 3-glucan;

FIG. 7 is a phenotype plot of pigment recruitment to the wound site following β -1, 3-glucan treatment;

FIG. 8 is a phenotype graph of angiogenesis after treatment of a test article;

FIG. 9 is the sum of the opacity of the wound site of zebrafish after treatment with β -1, 3-glucan;

FIG. 10 shows the wound healing promoting effect of beta-1, 3-glucan on zebrafish after treatment;

FIG. 11 is a graph of the incidence of neoangiogenesis in zebrafish after treatment with β -1, 3-glucan.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

First, preparation example:

preparation of beta-1, 3-glucan: