阅读说明：本技术 微生物快速检测膜及其制备方法和使用方法 (Microbial rapid detection membrane and preparation method and use method thereof ) 是由 沈叶青 于 2020-07-03 设计创作，主要内容包括：本发明涉及的微生物快速检测膜的制备方法如下：S1、将营养肉汤、蛋白胨、氯化钠、琼脂加入到无菌去离子水中,加热直至溶解得到溶液,调节溶液PH值,得到脂质体；S2、提供模具,将脂质体注入模具中,自然冷却得到微生物快速检测膜,该制备方法简单,材料易得,得到的微生物快速检测膜能够快速准确地检测到出多种常见的真菌和细菌,且操作简单、使用方便、环保、成本低,同时,能够快速检测抗菌剂的产品性能,可操作性强。(The preparation method of the microbial rapid detection membrane comprises the following steps: s1, adding the nutrient broth, peptone, sodium chloride and agar into sterile deionized water, heating until the nutrient broth, the peptone, the sodium chloride and the agar are dissolved to obtain a solution, and adjusting the pH value of the solution to obtain a liposome; s2, providing a mold, injecting the liposome into the mold, and naturally cooling to obtain the microorganism rapid detection membrane, wherein the preparation method is simple, the materials are easy to obtain, the obtained microorganism rapid detection membrane can rapidly and accurately detect various common fungi and bacteria, the operation is simple, the use is convenient, the environment is protected, the cost is low, meanwhile, the product performance of the antibacterial agent can be rapidly detected, and the operability is strong.)

1. A preparation method of a microbial rapid detection membrane is characterized by comprising the following steps:

s1, adding the nutrient broth, peptone, sodium chloride and agar into sterile deionized water, heating until the nutrient broth, the peptone, the sodium chloride and the agar are dissolved to obtain a solution, and adjusting the pH value of the solution to obtain a liposome;

s2, providing a mould, injecting the liposome into the mould, and naturally cooling to obtain the microbial rapid detection membrane.

2. The method for preparing a membrane for the rapid detection of microorganisms according to claim 1, wherein the amount of the nutrient broth is 3-5g, the amount of the peptone is 10-15g, the amount of the sodium chloride is 5-8.5g, the amount of the agar is 15-20g, and the amount of the sterile deionized water is 1000-1200 mL.

3. The method for preparing a membrane for rapid detection of microorganisms according to claim 1, wherein the heating temperature is 90 to 100 ℃ in step S1.

4. The method for preparing a membrane for rapid detection of microorganisms according to claim 1, wherein the method for adjusting the PH of the solution comprises: sodium hydroxide was added to the solution until the PH of the solution was 7.2 ± 0.2.

5. The method for preparing a membrane for rapid detection of microorganisms according to claim 1, wherein the membrane for rapid detection of microorganisms is any one of a rectangle, a square and a circle.

6. The method of preparing a rapid microbial detection membrane according to claim 1, wherein the thickness of the rapid microbial detection membrane is in the range of 2 to 8 mm.

7. The use method of the microorganism rapid detection membrane is characterized by comprising the following steps:

s1, contacting the microorganism rapid detection film with a sample to be detected, and attaching part of the sample to be detected on the microorganism rapid detection film;

s2, culturing the microorganism rapid detection film attached with the sample to be detected, and observing the growth condition of the microorganism on the surface of the microorganism rapid detection film.

8. The method for using a membrane for the rapid detection of microorganisms according to claim 7, wherein the membrane for the rapid detection of microorganisms is contacted with a sample to be tested for a period of time of 5 to 10 seconds.

9. The method of using the rapid microorganism detection membrane according to claim 7, wherein the culture conditions of the rapid microorganism detection membrane to which the sample to be tested is attached are as follows: culturing at 35 deg.C for 24-72h, or culturing at room temperature for 28-72 h.

10. A rapid microorganism detection membrane for detecting bacteria and/or fungi, which is obtained by the method for preparing the rapid microorganism detection membrane according to any one of claims 1 to 6.

Technical Field

The invention relates to a microbial rapid detection membrane and a preparation method and a use method thereof.

Background

With the increasing life of people, people pay more attention to the quality of life, and whether the control and treatment of infectious diseases or the monitoring and management of food safety need to reduce the contact of microorganisms harmful to human bodies. At present, in order to reduce the amount of microorganisms, bactericides or antibacterial agents are often used for sterilization, but in actual life, in order to determine the effect of the bactericides or antibacterial agents or determine whether bacteria or fungi are bred in a certain place, whether the microorganisms exist or not or whether the amount of the microorganisms exceeds the standard cannot be observed by naked eyes, the existing detection method is to send the object to be detected to a laboratory for detection, but the method is troublesome and time-consuming, and needs to rely on precise instruments and professionals, so that the detection cost is greatly increased.

Disclosure of Invention

The invention aims to provide a preparation method of a microorganism rapid detection membrane, which is simple in preparation method and easy in material obtaining, can rapidly detect various common fungi and bacteria, and is simple in operation, convenient to use, low in cost and environment-friendly.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a microbial rapid detection membrane comprises the following steps:

s1, adding the nutrient broth, peptone, sodium chloride and agar into sterile deionized water, heating until the nutrient broth, the peptone, the sodium chloride and the agar are dissolved to obtain a solution, and adjusting the pH value of the solution to obtain a liposome;

s2, providing a mould, injecting the liposome into the mould, and naturally cooling to obtain the microbial rapid detection membrane.

Further, the amount of the nutrient broth is 3-5g, the amount of the peptone is 10-15g, the amount of the sodium chloride is 5-8.5g, the amount of the agar is 15-20g, and the amount of the sterile deionized water is 1000-1200 mL.

Further, in step S1, the heating temperature is 90 to 100 ℃.

Further, the method for adjusting the pH value of the solution comprises the following steps: sodium hydroxide was added to the solution until the PH of the solution was 7.2 ± 0.2.

Further, the microorganism rapid detection membrane is any one of a rectangle, a square and a circle.

Further, the thickness range of the microorganism rapid detection membrane is 2-8 mm.

The invention also provides a use method of the microorganism rapid detection membrane, which comprises the following steps:

s1, contacting the microorganism rapid detection film with a sample to be detected, and attaching part of the sample to be detected on the microorganism rapid detection film;

s2, culturing the microorganism rapid detection film attached with the sample to be detected, and observing the growth condition of the microorganism on the surface of the microorganism rapid detection film.

Further, the contact time of the microorganism rapid detection membrane and a sample to be detected is 5-10 s.

Further, the culture conditions of the microorganism rapid detection membrane attached with the sample to be detected are as follows: culturing at 35 deg.C for 24-72h, or culturing at room temperature for 28-72 h.

The invention also provides a microorganism rapid detection membrane for detecting bacteria and/or fungi, and the microorganism rapid detection membrane is obtained by the preparation method of the microorganism rapid detection membrane.

The invention has the beneficial effects that: the preparation method of the rapid microorganism detection membrane provided by the invention is simple, materials are easy to obtain, the obtained rapid microorganism detection membrane can rapidly and accurately detect various common fungi and bacteria, the operation is simple, the use is convenient, the environment is protected, the cost is low, meanwhile, the product performance of the antibacterial agent can be rapidly detected, and the operability is strong.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.

Drawings

FIG. 1 is a flow chart of the preparation of a rapid microbial detection membrane according to an embodiment of the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

The microorganism rapid detection membrane disclosed by the invention is used for detecting common bacteria and/or fungi in life, such as: escherichia coli, Staphylococcus aureus, Streptococcus, Mucor, Penicillium, Aspergillus, etc., but are not limited thereto. The microorganism rapid detection membrane may be any one of a rectangle, a square and a circle, but is not limited thereto, and the microorganism rapid detection membrane may be prepared in any desired shape, and is not particularly limited thereto. The thickness range of the microorganism rapid detection film is 2-8mm, and the size range is 1-5cm, but the invention is not limited to the thickness range, and the specific size can be prepared according to the actual requirement.

Referring to fig. 1, the present invention further provides a method for preparing the above-mentioned rapid detection membrane for microorganisms, which comprises the following steps:

s1, adding the nutrient broth, peptone, sodium chloride and agar into sterile deionized water, heating until the nutrient broth, the peptone, the sodium chloride and the agar are dissolved to obtain a solution, and adjusting the pH value of the solution to obtain a liposome;

and S2, providing a mold, injecting the liposome into the mold, and naturally cooling to obtain the microbial rapid detection membrane.

Wherein, the amount of the nutrient broth is 3-5g, the amount of the peptone is 10-15g, the amount of the sodium chloride is 5-8.5g, the amount of the agar is 15-20g, the amount of the sterile deionized water is 1000-1200mL, and the specific usage amounts of the nutrient broth, the peptone, the sodium chloride, the agar and the sterile deionized water can be selected according to actual needs, and are not particularly limited herein.

The obtained mixture is heated at 90-100 deg.C by heating device such as heating jacket to accelerate dissolution of nutrient broth, peptone, sodium chloride, and agar in sterile deionized water.

The method for adjusting the pH value of the solution comprises the following steps: sodium hydroxide was added to the solution obtained by heating until the pH of the solution was 7.2. + -. 0.2. Indeed, in other embodiments, other alkaline substances may be used to adjust the PH, and are not specifically limited herein.

A plurality of grooves can be formed in one die, and the grooves can be formed according to the structure and the size of the microbial rapid detection film in actual needs, so that the microbial rapid detection film can be produced in batches.

It should be noted that, when preparing the rapid microorganism detection membrane, the whole process is performed in a sterile environment, and all the used tools, such as molds, should be sterilized.

The treatment method of the sterile deionized water comprises the following steps: passing pure water through a polymer permeable membrane, performing reverse osmosis to remove ions contained in the water to obtain deionized water, and sterilizing with an autoclave to obtain sterile deionized water, wherein the temperature is 121 deg.C, the pressure is 0.15Mpa, and the sterilization treatment time is 20-25 min. The sterilization method of the deionized water is not particularly limited.

The following will describe in detail the preparation method of the membrane for rapid detection of microorganisms by using specific examples: