阅读说明：本技术 确定细胞样品中靶核酸存在或不存在的方法 (Method for determining the presence or absence of a target nucleic acid in a cell sample ) 是由 M·斯普林格-豪瑟斯 C·库普弗 P·沙茨 于 2013-08-16 设计创作，主要内容包括：确定细胞样品中靶核酸存在或不存在的方法,所述方法包括：a)将含有阴离子交换部分的表面与样品在适合诱导所述细胞和表面之间结合的条件下接触；b)将结合有细胞的表面与剩余样品分离以收集细胞；c)从细胞释放核酸；d)在靶核酸和靶核酸的特异性探针之间形成杂交体；和e)检测所述杂交体的存在或不存在。本发明提供快速和可自动化的方法,其中通过与阴离子交换表面的结合从周围的液体介质,如液基细胞学介质细胞收集细胞,如源自子宫颈拭子样品的上皮细胞。细胞以高亲和力和快速动力学特征直接结合于优选由携带阴离子交换部分的磁性颗粒提供的阴离子交换表面。结合于阴离子交换表面的细胞易与周围介质分离,可以直接重悬于适合在步骤d)中进行的后续杂交体捕获测定的组合物以检测,例如病原体核酸,如HPV核酸。(A method of determining the presence or absence of a target nucleic acid in a cell sample, the method comprising: a) contacting a surface comprising an anion exchange moiety with a sample under conditions suitable to induce binding between the cell and the surface; b) separating the cell-bound surface from the remaining sample to collect cells; c) releasing the nucleic acid from the cell; d) forming a hybrid between the target nucleic acid and the probe specific for the target nucleic acid; and e) detecting the presence or absence of the hybrid. The present invention provides a rapid and automatable method wherein cells, such as epithelial cells derived from a cervical swab sample, are collected from surrounding liquid medium, such as liquid-based cytological medium cells, by binding to an anion exchange surface. Cells bind directly with high affinity and fast kinetic characteristics to the anion exchange surface preferably provided by the magnetic particles carrying the anion exchange moiety. The cells bound to the anion exchange surface are easily separated from the surrounding medium and can be directly resuspended in a composition suitable for the subsequent hybrid capture assay performed in step d) for detection of, for example, pathogen nucleic acids, such as HPV nucleic acids.)