阅读说明：本技术 一种连续进样层析装置及其进样控制方法 (Continuous sample introduction chromatography device and sample introduction control method thereof ) 是由 岑云东 李泓文 张佶 聂大林 于 2020-05-21 设计创作，主要内容包括：本发明公开了一种连续进样层析装置及其进样控制方法,包括多组层析组件、至少一个柱前多通阀、至少一个柱后多通阀,每组层析组件包括一个层析柱、设置在层析柱前端的柱前柱选择阀及设置在层析柱后端的柱后柱选择阀,所述柱前柱选择阀和柱后柱选择阀分别具有多个与所述层析柱连通的通道口,不同的层析组件中的柱前柱选择阀与柱后柱选择阀错峰连接,包括：一组层析组件中的柱前柱选择阀的一个柱前通道口与另一组层析组件中的柱后柱选择阀的一个柱后通道口连接。本发明提供的装置可任意选择层析柱的串联排列顺序,能同时实现各层析柱的单向冲洗和逆向冲洗,控制逻辑关系较简单,稳定性高不易出错。(The invention discloses a continuous sample introduction chromatography device and a sample introduction control method thereof, wherein the device comprises a plurality of groups of chromatography components, at least one front column multi-way valve and at least one rear column multi-way valve, each group of chromatography components comprises a chromatography column, a front column selection valve arranged at the front end of the chromatography column and a rear column selection valve arranged at the rear end of the chromatography column, the front column selection valve and the rear column selection valve are respectively provided with a plurality of channel ports communicated with the chromatography column, and the front column selection valves and the rear column selection valves in different chromatography components are in peak staggering connection, and the device comprises: one of the pre-column ports of the pre-column selection valves in one set of chromatography modules is connected to one of the post-column ports of the post-column selection valves in the other set of chromatography modules. The device provided by the invention can randomly select the series arrangement sequence of the chromatographic columns, can simultaneously realize the one-way washing and the reverse washing of each chromatographic column, has simpler control logic relationship and high stability, and is not easy to make mistakes.)

1. A continuous sample introduction chromatography device is characterized by comprising a plurality of groups of chromatography components, at least one front column multi-way valve (2) and at least one rear column multi-way valve (3), wherein each group of chromatography components comprises a chromatography column (12), a front column selection valve (11) arranged at the front end of the chromatography column (12) and a rear column selection valve (13) arranged at the rear end of the chromatography column (12), the front column selection valve (11) and the rear column selection valve (13) are respectively provided with a plurality of channel ports communicated with the chromatography column (12), the front end of the chromatography column (12) is communicated with a front column central hole (110) of the front column selection valve (11), the front column central hole (110) is controllably communicated with one of the front column channel ports of the front column selection valve (11), the rear end of the chromatography column (12) is communicated with a rear column selection valve (130) of the rear column selection valve (13), the post central hole (130) is controllably communicated with one of a plurality of post channel ports of a post selection valve (13);

one port of the front column multi-way valve (2) is communicated with the power pump, and other ports are respectively communicated with a front column channel port of the front column selection valve (11); one port of the post-column multi-way valve (3) is communicated with the first waste liquid port (51) or the detection device (6), and other ports are respectively communicated with a post-column channel port of the post-column selection valve (13);

the pre-column selection valve (11) and the post-column selection valve (13) in different chromatography components are connected in a staggered mode, and the method comprises the following steps: one of the pre-column ports of the pre-column selection valve (11) in one of the chromatography modules is connected to one of the post-column ports of the post-column selection valve (13) in the other chromatography module.

2. The continuous sample introduction chromatography device according to claim 1, wherein the chromatography columns (12) of the multiple sets of chromatography modules are connected in series in the peak-shifted connection state of the column front selection valve (11) and the column rear selection valve (13) in different chromatography modules.

3. The continuous sample introduction chromatography device according to claim 1, wherein the pre-column multi-way valve (2) and the post-column multi-way valve (3) have the same number of passages or different numbers of passages, the less pre-column multi-way valve (2) or the post-column multi-way valve (3) has n passages, and the chromatography component is less than or equal to n-1 groups.

4. The continuous sample introduction chromatography device according to claim 1, wherein the number of the pre-column multi-way valves (2) and the post-column multi-way valves (3) is at least two, the first pre-column multi-way valve (2) is communicated with the sample pump (41), and the second pre-column multi-way valve (2) is communicated with the system pump (42); the first post multi-way valve (3) is communicated with the first waste liquid port (51), and the second post multi-way valve (3) is communicated with the detection device (6).

5. The continuous sample introduction chromatography device according to claim 1, wherein at least one pre-column multi-way valve (2) is in communication with the purge pump (43) through a reversing valve (71), the pre-column multi-way valve (2) is also in communication with the second waste liquid port (52) through a back pressure valve (72);

at least one post multi-way valve (3) is communicated with the cleaning pump (43) through the reversing valve (71).

6. The continuous sample introduction chromatography device as claimed in claim 1, wherein the pre-column multi-way valve (2) comprises a first pre-column n-way valve, a second pre-column n-way valve and a third pre-column n + 1-way valve, and the post-column multi-way valve (3) comprises a first post-column n-way valve, a second post-column n-way valve and a third post-column n-way valve;

the chromatography components comprise a first chromatography component, a second chromatography component and an n-1 chromatography component;

the n passages of the first pre-column n-way valve are respectively communicated with a sample pump (41) and n-1 pre-column selection valves (11), the n passages of the second pre-column n-way valve are respectively communicated with a system pump (42) and n-1 pre-column selection valves (11), the n +1 passages of the third pre-column n + 1-way valve are respectively communicated with a cleaning pump (43), a second waste liquid port (52) and n-1 pre-column selection valves (11), wherein a reversing valve (71) is arranged between the third pre-column n + 1-way valve and the cleaning pump (43), and a back pressure valve (72) is arranged between the third pre-column n + 1-way valve and the second waste liquid port (52);

the n passages of the first post n-way valve are respectively communicated with a first waste liquid port (51) and n-1 post selection valves (13), the n passages of the second post n-way valve are respectively communicated with a UV detection device and n-1 post selection valves (13), and the n passages of the third post n-way valve are respectively communicated with the reversing valve (71) and n-1 post selection valves (13).

7. The continuous sample feeding chromatography device according to claim 1, wherein the chromatography component is n-1 group, wherein n is more than or equal to 4; and a front column channel port of the first chromatography component is communicated with a rear column channel port of the adjacent second chromatography component, a front column channel port of the second chromatography component is communicated with a rear column channel port of the adjacent third chromatography component, and the front column channel port of the second chromatography component and the rear column channel port of the adjacent third chromatography component are sequentially connected until a front column channel port of the (n-1) th chromatography component is communicated with a rear column channel port of the first chromatography component.

8. The continuous sample feeding chromatography device according to claim 1, wherein the chromatography component is n-1 group, wherein n is more than or equal to 4; a front column channel port of the first chromatography component is communicated with a rear column channel port of the third chromatography component, a front column channel port of the second chromatography component is communicated with a rear column channel port of the fourth chromatography component until a front column channel port of the (n-2) th chromatography component is communicated with a rear column channel port of the first chromatography component, and a front column channel port of the (n-1) th chromatography component is communicated with a rear column channel port of the second chromatography component.

9. A sample injection control method based on the continuous sample injection chromatography device as claimed in any one of claims 1 to 8, comprising: a sample to be injected enters one or more groups of chromatographic assemblies through a corresponding pre-column multi-way valve and one or more preset pre-column selection valves under the action of a power pump, and then enters a first waste liquid port and/or a detection device through one or more preset post-column selection valves and corresponding post-column multi-way valves;

wherein the control of the sample into the plurality of sets of chromatography modules comprises: the sample enters different chromatographic assemblies through different power pumps, or the sample enters different chromatographic assemblies in sequence through the peak shifting connection of different chromatographic assemblies.

10. The sample injection control method according to claim 9, wherein based on the continuous sample injection chromatography device of claim 6, the sample is controlled to enter the chromatography module under the action of the power pump, and flow through the chromatography column (12) from the front end to the rear end; alternatively, the first and second electrodes may be,

and controlling the direction of the reversing valve, enabling the sample to sequentially pass through the cleaning pump and the third column, then enter the chromatography component through the n-way valve, and flow through the chromatography column from the rear end to the front end of the chromatography column (12).

Technical Field

The invention relates to the field of chromatography devices, in particular to a continuous sample introduction chromatography device and a sample introduction control method thereof.

Background

The liquid chromatography apparatus separates various substances to be separated by utilizing the difference of affinity such as partition coefficient, adsorption capacity and the like of the substances in two phases. The sample introduction chromatography device used in the prior art generally comprises the following processes: 1. the method comprises the following five steps of balancing, 2, loading, 3, eluting, 4, flushing and 5, wherein the five steps are repeated for each loading, and the whole process is time-consuming and easy to generate bubbles. Furthermore, when a plurality of chromatography columns are provided in a flow path, the above-described five steps are repeated for each switching, which is more time-consuming.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention provides a continuous sample injection chromatography device and a sample injection control method thereof, and the technical scheme is as follows:

on one hand, the invention provides a continuous sample introduction chromatography device, which comprises a plurality of groups of chromatography components, at least one front column multi-way valve and at least one rear column multi-way valve, wherein each group of chromatography components comprises a chromatography column, a front column selection valve arranged at the front end of the chromatography column and a rear column selection valve arranged at the rear end of the chromatography column, the front column selection valve and the rear column selection valve are respectively provided with a plurality of channel ports communicated with the chromatography column, the front end of the chromatography column is communicated with a front central hole of the front column selection valve, the front central hole of the column is controllably communicated with one of the front column channel ports of the front column selection valve, the rear end of the chromatography column is communicated with a rear central hole of the rear column selection valve, and the rear central hole of the column is controllably communicated with one of the rear column channel ports of the rear column selection valve;

one port of the front column multi-way valve is communicated with the power pump, and the other ports are respectively communicated with a front column channel port of the front column selection valve; one port of the post-column multi-way valve is communicated with the first waste liquid port or the detection device, and other ports are respectively communicated with a post-column channel port of the post-column selection valve;

the pre-column selection valve and post-column selection valve in different chromatography modules are connected in a peak staggering way, and the method comprises the following steps: one of the pre-column ports of the pre-column selection valves in one set of chromatography modules is connected to one of the post-column ports of the post-column selection valves in the other set of chromatography modules.

Further, the chromatographic columns of the multiple sets of chromatographic assemblies can be connected in series under the condition that the column front selection valves and the column rear selection valves in different chromatographic assemblies are in a peak shifting connection state.

Further, the pre-column multi-way valve and the post-column multi-way valve have the same number of passages or different numbers of passages, the less pre-column multi-way valve or the post-column multi-way valve has n passages, and the number of the chromatography components is less than or equal to n-1 groups.

Furthermore, the number of the front multi-way valves of the column and the number of the rear multi-way valves of the column are at least two, the first front multi-way valve of the column is communicated with the sample pump, and the second front multi-way valve of the column is communicated with the system pump; the first post multi-way valve is communicated with the first waste liquid port, and the second post multi-way valve is communicated with the detection device.

Furthermore, at least one front multi-way valve is communicated with the cleaning pump through a reversing valve, and the front multi-way valve is also communicated with a second waste liquid port through a back pressure valve;

and at least one post multi-way valve is communicated with the cleaning pump through the reversing valve.

The post multi-way valve comprises a first post n-way valve, a second post n-way valve and a third post n + 1-way valve, and the post multi-way valve comprises a first post n-way valve, a second post n-way valve and a third post n-way valve;

the chromatography components comprise a first chromatography component, a second chromatography component and an n-1 chromatography component;

the n passages of the first front column n-way valve are respectively communicated with a sample pump and n-1 front column selection valves, the n passages of the second front column n-way valve are respectively communicated with a system pump and n-1 front column selection valves, the n +1 passages of the third front column n + 1-way valve are respectively communicated with a cleaning pump, a second waste liquid port and n-1 front column selection valves, wherein a reversing valve is arranged between the third front column n + 1-way valve and the cleaning pump, and a back pressure valve is arranged between the third front column n + 1-way valve and the second waste liquid port;

the n passages of the first post n-way valve are respectively communicated with a first waste liquid port and n-1 post selection valves, the n passages of the second post n-way valve are respectively communicated with a UV detection device and n-1 post selection valves, and the n passages of the third post n-way valve are respectively communicated with the reversing valve and n-1 post selection valves.

Further, the chromatography component is n-1 group, wherein n is more than or equal to 4; and a front column channel port of the first chromatography component is communicated with a rear column channel port of the adjacent second chromatography component, a front column channel port of the second chromatography component is communicated with a rear column channel port of the adjacent third chromatography component, and the front column channel port of the second chromatography component and the rear column channel port of the adjacent third chromatography component are sequentially connected until a front column channel port of the (n-1) th chromatography component is communicated with a rear column channel port of the first chromatography component.

Further, the chromatography component is n-1 group, wherein n is more than or equal to 4; a front column channel port of the first chromatography component is communicated with a rear column channel port of the third chromatography component, a front column channel port of the second chromatography component is communicated with a rear column channel port of the fourth chromatography component until a front column channel port of the (n-2) th chromatography component is communicated with a rear column channel port of the first chromatography component, and a front column channel port of the (n-1) th chromatography component is communicated with a rear column channel port of the second chromatography component.

In another aspect, the present invention provides a sample injection control method for a continuous sample injection chromatography device, including: a sample to be injected enters one or more groups of chromatographic assemblies through a corresponding pre-column multi-way valve and one or more preset pre-column selection valves under the action of a power pump, and then enters a first waste liquid port and/or a detection device through one or more preset post-column selection valves and corresponding post-column multi-way valves;

wherein the control of the sample into the plurality of sets of chromatography modules comprises: the sample enters different chromatographic assemblies through different power pumps, or the sample enters different chromatographic assemblies in sequence through the peak shifting connection of different chromatographic assemblies.

Further, controlling the sample to enter the chromatography component under the action of the power pump, and flowing through the chromatography column from the front end to the rear end of the chromatography column; alternatively, the first and second electrodes may be,

and controlling the direction of the reversing valve, enabling the sample to sequentially pass through the cleaning pump and the third column, then enter the chromatographic assembly through the n-way valve, and flow through the chromatographic column from the rear end to the front end of the chromatographic column.

The technical scheme provided by the invention has the following beneficial effects:

a. when a plurality of chromatographic columns are required to be loaded and eluted simultaneously, the serial arrangement sequence of the chromatographic columns can be selected randomly;

b. the sample loading, elution and cleaning are respectively carried out by a sample pump, a system pump and a cleaning pump, and the one-way flushing and the reverse flushing of each chromatographic column can be simultaneously realized;

only one group of UV detection devices is needed, so that the cost is saved;

d. the number of the valves is small, the control logic relation is simple, the stability is high, and errors are not prone to occurring.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a block diagram of a module of a continuous sample injection chromatography apparatus provided in an embodiment of the present invention;

FIG. 2 is a schematic diagram of the adjacent connection of chromatographic modules provided in an embodiment of the present invention;

FIG. 3 is a schematic view of the chromatographic assembly provided by the embodiment of the present invention separated by one chromatographic assembly connection;

FIG. 4 is a pump-to-detection apparatus roadmap for a system provided by an embodiment of the present invention;

FIG. 5 is a schematic diagram of a system pump-to-waste port provided by an embodiment of the present invention;

FIG. 6 is a schematic of a sample pump to detection apparatus scheme provided by an embodiment of the present invention;

FIG. 7 is a sample pump to waste port line diagram provided by an embodiment of the present invention;

FIG. 8 is a schematic of a system pump according to an embodiment of the present invention pumping through two tandem chromatography modules to a detection device;

FIG. 9 is a schematic of a sample pump through three tandem chromatography modules to a detection apparatus according to an embodiment of the invention;

FIG. 10 is a forward purge route diagram for a purge pump provided by an embodiment of the present invention;

fig. 11 is a schematic diagram of a reverse cleaning route for a cleaning pump according to an embodiment of the present invention.

Wherein the reference numerals include: 11-column front column selection valve, 110-column front center hole, 111-column front channel port, 112-column front channel port, 113-column front channel port, 114-column front channel port, 115-column front channel port, 116-column front channel port, 12-chromatography column, 13-column rear column selection valve, 2-column front multi-way valve, 3-column rear multi-way valve, 130-column rear center hole, 131-column rear channel port, 132-column rear channel port, 133-column rear channel port, 134-column rear channel port, 135-column rear channel port, 136-column rear channel port, 41-sample pump, 42-system pump, 43-cleaning pump, 51-first waste liquid port, 52-second waste liquid port, 6-detection device, 71-reversing valve, 72-back pressure valve.

Detailed Description

In order to make the technical solutions of the present invention better understood and more clearly understood by those skilled in the art, the technical solutions of the embodiments of the present invention will be described below in detail and completely with reference to the accompanying drawings. It should be noted that the implementations not shown or described in the drawings are in a form known to those of ordinary skill in the art. Additionally, while exemplifications of parameters including particular values may be provided herein, it is to be understood that the parameters need not be exactly equal to the respective values, but may be approximated to the respective values within acceptable error margins or design constraints. It is to be understood that the described embodiments are merely exemplary of a portion of the invention and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. In addition, the terms "comprises" and "comprising," and any variations thereof, in the description and claims of this invention, are intended to cover a non-exclusive inclusion, such that a process, method, apparatus, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

In one embodiment of the present invention, a continuous sample introduction chromatography device is provided, referring to fig. 1, comprising a plurality of sets of chromatography components, at least one pre-column multi-way valve 2 and at least one post-column multi-way valve 3; the pre-column multi-way valve 2 and the post-column multi-way valve 3 have the same number of passages or different numbers of passages, and when the least number of pre-column multi-way valves 2 or post-column multi-way valves 3 have n passages, the number of chromatography components is less than or equal to n-1 groups; each group of chromatography components comprises a chromatography column 12, a pre-column selection valve 11 arranged at the front end of the chromatography column 12 and a post-column selection valve 13 arranged at the rear end of the chromatography column 12, wherein the pre-column selection valve 11 and the post-column selection valve 13 are respectively provided with a plurality of passage ports communicated with the chromatography column 12, the front end of the chromatography column 12 is controllably communicated with one of the pre-column passage ports of the pre-column selection valve 11, and the rear end of the chromatography column 12 is controllably communicated with one of the post-column passage ports of the post-column selection valve 13;

one port of the front column multi-way valve 2 is communicated with the power pump, and the other ports are respectively communicated with a front column channel port of the front column selection valve 11; one of the ports of the post-column multi-way valve 3 is used for being communicated with the first waste liquid port 51 or the detection device 6, and the other ports are respectively used for being communicated with the post-column channel port of the post-column selection valve 13;

the pre-column selection valve 11 and the post-column selection valve 13 in different chromatography modules are connected in a staggered mode, and comprise: one of the pre-column ports of the pre-column selector valve 11 in one set of chromatography modules is connected to one of the post-column ports of the post-column selector valve 13 in the other set of chromatography modules. The columns 12 of the multiple sets of chromatography modules can be connected in series in the above-described peak-off connection state. The following examples are two peak-shifting connection methods of adjacent chromatography modules and 1 chromatography module, but the peak-shifting connection method of the present invention includes the two peak-shifting connection methods but is not limited to the two methods, and also includes a method of connecting the chromatography modules in series with other number of chromatography modules or with different number of chromatography modules.

When adjacent chromatographic components are in peak staggering connection, the chromatographic components are n-1 groups, wherein n is more than or equal to 5; and a front column channel port of the first chromatography component is communicated with a rear column channel port of the adjacent second chromatography component, a front column channel port of the second chromatography component is communicated with a rear column channel port of the adjacent third chromatography component, and the front column channel port of the second chromatography component and the rear column channel port of the adjacent third chromatography component are sequentially connected until a front column channel port of the (n-1) th chromatography component is communicated with a rear column channel port of the first chromatography component. Taking an example when n is 5, referring to fig. 2, one pre-column port (port 4 in the figure) of the first chromatography module communicates with one post-column port (port 4 in the figure) of the adjacent second chromatography module, one pre-column port of the second chromatography module communicates with one post-column port of the adjacent third chromatography module, one pre-column port of the third chromatography module communicates with one post-column port of the adjacent fourth chromatography module, one pre-column port of the fourth chromatography module communicates with one post-column port of the first chromatography module, and the channels are marked by triangles in fig. 2.

When the chromatographic components are connected in a staggered mode at intervals, the chromatographic components are n-1 groups, wherein n is more than or equal to 5; a front column channel port of the first chromatography component is communicated with a rear column channel port of the third chromatography component, a front column channel port of the second chromatography component is communicated with a rear column channel port of the fourth chromatography component, and the front column channel port of the n-2 th chromatography component is communicated with a rear column channel port of the first chromatography component and the front column channel port of the n-1 th chromatography component is communicated with a rear column channel port of the second chromatography component. In the following, an example is given when n is 5, that is, there are four sets of chromatography modules, referring to fig. 3, where one pre-column channel port (port 5 in the figure) of the first chromatography module communicates with one post-column channel port (port 5 in the figure) of the third chromatography module, one pre-column channel port of the second chromatography module communicates with one post-column channel port of the fourth chromatography module, one pre-column channel port of the third chromatography module communicates with one post-column channel port of the first chromatography module, one pre-column channel port of the fourth chromatography module communicates with one post-column channel port of the second chromatography module, and the channels are marked by double-slashes in fig. 3.

In one embodiment of the present invention, at least one of the pre-column multi-way valves 2 is further communicated with the purge pump 43 through a reversing valve 71, and the pre-column multi-way valve 2 is further communicated with the second waste liquid port 52 through a back pressure valve 72; at least one post multi-way valve 3 is communicated with the cleaning pump 43 through the reversing valve 71, and reverse cleaning can be realized, and the specific process is described in detail below.

In one embodiment of the invention, the front pillar multi-way valve 2 comprises a first front pillar n-way valve, a second front pillar n-way valve and a third front pillar n + 1-way valve, and the rear pillar multi-way valve 3 comprises a first rear pillar n-way valve, a second rear pillar n-way valve and a third rear pillar n-way valve;

the chromatography components comprise a first chromatography component, a second chromatography component and an n-1 chromatography component;

the n passages of the first pre-column n-way valve are respectively communicated with a sample pump 41 and n-1 pre-column selection valves 11, the n passages of the second pre-column n-way valve are respectively communicated with a system pump 42 and n-1 pre-column selection valves 11, the n +1 passages of the third pre-column n + 1-way valve are respectively communicated with a cleaning pump 43, a second waste liquid port 52 and n-1 pre-column selection valves 11, wherein a reversing valve 71 is arranged between the third pre-column n + 1-way valve and the cleaning pump 43, and a back pressure valve 72 is arranged between the third pre-column n + 1-way valve and the second waste liquid port 52;

the n passages of the first post n-way valve are respectively communicated with a first waste liquid port 51 and n-1 post selection valves 13, the n passages of the second post n-way valve are respectively communicated with a UV detection device and n-1 post selection valves 13, and the n passages of the third post n-way valve are respectively communicated with the reversing valve 71 and n-1 post selection valves 13.

In one embodiment of the present invention, a sample injection control method for a continuous sample injection chromatography device is provided, which includes: a sample to be injected enters one or more groups of chromatographic assemblies through the power pump and the front multi-way valve of the column in sequence, and then enters the first waste liquid port and/or the detection device through one or more rear multi-way valves of the column;

wherein the control of the sample into the plurality of sets of chromatography modules comprises: the sample enters different chromatographic assemblies through different power pumps, or the sample enters different chromatographic assemblies in sequence through the peak shifting connection of different chromatographic assemblies.

In the following, using a continuous sample injection chromatography apparatus comprising 4 sets of chromatography components, 2 pre-column five-way valves (valve a and valve C), 1 pre-column six-way valve (valve B), and 3 post-column five-way valves (valve a, valve B, and valve C) as an example for explanation, the 4 pre-column selection valves 11 corresponding to the 4 sets of chromatography components are a pre-column selection valve i, a pre-column selection valve ii, a pre-column selection valve iii, and a pre-column selection valve iv, the corresponding 4 post-column selection valves 13 are a post-column selection valve V, a post-column selection valve VI, a post-column selection valve VII and a post-column selection valve VIII, respectively, as shown in FIG. 1, wherein, one port of the pre-column five-way valve a is connected with the sample pump 41, and the other four ports are respectively communicated with one pre-column channel port (such as the third pre-column channel port 113 in fig. 1) of 4 pre-column selection valves 11 (pre-column selection valves i-iv) in 4 sets of chromatographic assemblies; one port of another pre-column five-way valve c is connected to the system pump 42, the remaining four ports are respectively communicated with another pre-column channel port (for example, the second pre-column channel port 112 in fig. 1) of 4 pre-column selection valves 11 (pre-column selection valves i-iv) in the 4 sets of chromatographic assemblies, one port of a pre-column six-way valve b is connected to the purge pump 43 through the reversing valve 71, the other port is communicated with the second waste liquid port 52 through the backpressure valve 72, the remaining four ports are respectively communicated with another pre-column channel port (for example, the first pre-column channel port 111 in fig. 1) of the 4 pre-column selection valves 11 (pre-column selection valves i-iv) in the 4 sets of chromatographic assemblies, it should be noted that the same pre-column channel port of the pre-column selection valve 11 is at most communicated with only one fluid channel, and the post-column channel ports of the post-column selection valve 13 are in the same order; one port of one post-column five-way valve a is connected to the waste liquid port, and the other four ports are connected to one post-column channel port (for example, post-column channel port 131 in fig. 1) of 4 post-column selection valves 13 (post-column selection valves v-viii) in the 4 sets of chromatographic assemblies; one port of the second post-column five-way valve B is connected to the UV detection device 6, and the remaining four ports are connected to another post-column port (for example, the second post-column port 132 in fig. 1) of 4 post-column selection valves 13 (post-column selection valves v to viii) in the 4 sets of chromatographic modules; one port of the third post five-way valve C is connected to the aforementioned direction changing valve 71, and the remaining four ports are connected to one post port (for example, the third post port 133 in fig. 1) of the 4 post selection valves 13 (post selection valves v to viii) in the 4 sets of chromatography modules, and it is obvious that in this embodiment, at least three post ports are required for the pre-post selection valve 11 and the post selection valve 13, respectively. It should be noted that other combinations of the devices of the present invention without substantial effort are also within the scope of the present invention.

For example, in a preferred embodiment of the present invention, the pre-column selection valve 11 is provided with four pre-column ports, and the post-column selection valve 13 is provided with four post-column ports, i.e. the four pre-column port 114 and the four post-column port 134 are provided on the basis of the one-to-three pre-column ports and the one-to-three post-column ports, so that the chromatography apparatus can additionally implement a peak-shifting connection mode to connect a plurality of chromatography columns 12 in series on the basis of the functions of the above-mentioned embodiment.

In a preferred embodiment of the present invention, the pre-column selection valve 11 is provided with five pre-column ports, and the post-column selection valve 13 is provided with five post-column ports, that is, a five pre-column port 115 and a five post-column port 135, so that the chromatography device can additionally realize two off-peak connection modes to connect a plurality of chromatography columns 12 in series.

In a preferred embodiment of the present invention, the pre-column selection valve 11 is provided with six pre-column ports (from the first pre-column port 111 to the sixth pre-column port 116 arranged clockwise from nine o' clock), and the post-column selection valve 13 is provided with six post-column ports (from the first post-column port 131 to the sixth post-column port 136), wherein the sixth pre-column port 116 and the sixth post-column port 136 are empty, that is, under software control, if the pre-column central hole 110 (communicated with the chromatographic column 12) of the pre-column selection valve 11 is communicated with the empty sixth pre-column port 116, it means that the sample in the chromatographic column 12 is prevented from flowing forward; if the post-column central hole 130 of the post-column selector valve 13 communicates with the empty post-column channel No. six port 136, it means that the sample in the chromatography column 12 is prevented from flowing in the post-column direction. The above-mentioned number of the pre-column port and the post-column port is the number of ports other than the center holes (the pre-column center hole 110 and the post-column center hole 130) communicating with the chromatography column 12.

The invention includes the following modes of use of the embodiments, but is not limited to the following usage scenarios:

in an embodiment of the present invention, referring to fig. 4, when the system pump 42 is used to send the liquid to the detection device 6 for detection, the conducting state of each front column channel port in the front column selection valve and each rear column channel port in the rear column selection valve is controlled according to software, so that the liquid to be detected is pumped into one or more chromatography components by the system pump 42 through the front five-way valve c, and flows from the front end to the rear end of the chromatography components, at this time, the rear column central hole 130 of the rear column selection valve 13 is controlled to communicate with the second rear column channel port 132 in fig. 4, that is, the liquid can be collected and flow into the UV detection device 6 through the rear five-way valve B connected with the UV detection device. It should be noted that, only the chromatographic apparatus used in the context of this embodiment is shown in fig. 4, which may be the entire chromatographic apparatus or a partial structure of the chromatographic apparatus, and the same is as follows.

In an embodiment of the present invention, referring to fig. 5, when the system pump 42 is used to send the liquid to the waste liquid port for disposal, the conducting state of each pre-column channel port in the pre-column selection valve and each post-column channel port in the post-column selection valve is controlled according to the software, so that the liquid flows from the front end to the back end of the chromatography module after being pumped into one or more chromatography modules by the system pump 42 through the pre-column five-way valve c, and at this time, the post-column central hole 130 of the post-column selection valve 13 is controlled to communicate with the post-column channel port 131 in fig. 5, i.e. the liquid can be collectively flowed into the waste liquid port through the post-column five-way valve a connected with the first waste liquid port 51.

In an embodiment of the present invention, referring to fig. 6, when the sample pump 41 is used to send the liquid to the detection device 6 for detection, the conducting state of each front column channel port in the front column selection valve and each rear column channel port in the rear column selection valve is controlled by using software, so that the liquid to be detected is pumped into one or more chromatography components by the sample pump 41 through the front five-way valve a, and flows from the front end to the rear end of the chromatography components, at this time, the rear column central hole 130 of the rear column selection valve 13 is controlled to communicate with the second rear column channel port 132 in fig. 6, that is, the liquid can flow into the UV detection device 6 through the rear five-way valve B connected with the UV detection device 6 in a collective manner.

In one embodiment of the present invention, referring to fig. 7, when the sample pump 41 is used to send the liquid to the waste liquid port for disposal, the conducting state of each pre-column channel port in the pre-column selection valve and each post-column channel port in the post-column selection valve is controlled according to the software, so that the liquid flows from the front end to the back end of the chromatography module after being pumped into one or more chromatography modules by the sample pump 41 through the pre-column five-way valve a, and the liquid is then controlled to flow into the first waste liquid port 51 after being collected by the post-column five-way valve a connected to the waste liquid port while the post-column central hole 130 of the post-column selection valve 13 is communicated with the first post-column channel port 131 in fig. 7.

In one embodiment of the present invention, referring to fig. 8, when the system pump 42 is used to pass through two serially connected chromatography modules to the detection apparatus 6, according to software control, the front column port 114 of the front column selection valve iii of the third chromatography module and the rear column port 134 of the rear column selection valve viii of the fourth chromatography module are connected, the liquid to be detected is pumped into the front column port 112 of the front column selection valve iv of the fourth chromatography module through the front five way valve c of the column by the system pump 42, the liquid flows from the front end to the rear end of the fourth chromatography module and then flows from the rear column port 134 of the fourth chromatography module to the front column port 114 of the front column selection valve vii of the third chromatography module, so that the liquid flows from the front end to the rear end of the third chromatography module, the second chromatography is completed, and at this time, the rear column port 130 of the rear column selection valve 13 is connected to the rear column port 132 of the rear column selection valve vii of the rear column selection valve in fig. 8, and finally flows into the UV detection device 6 through a post five-way valve B connected with the UV detection device 6.

In one embodiment of the present invention, referring to fig. 9, when the sample pump 41 is used to pass through three chromatography modules connected in series to the detection apparatus 6, the communication between the fifth column front port 115 of the column front column selection valve i of the first chromatography module and the fifth column rear port 135 of the column rear column selection valve vii of the third chromatography module and between the fourth column front port 114 of the column front column selection valve iv of the fourth chromatography module and the fourth column rear port 134 of the column rear column selection valve v of the first chromatography module is controlled by using software, the liquid to be measured is pumped into the third column front port 113 of the third chromatography module by the sample pump 41 through the column front five way valve a, the liquid flows from the front end to the rear end of the third chromatography module, and then flows from the fifth column rear port 135 of the third chromatography module to the fifth column front port 115 of the first chromatography module, so that the liquid flows from the front end to the rear end of the first chromatography module, and (3) finishing the second chromatography, then flowing from the fourth post-column channel port 134 of the first chromatography component to the fourth pre-column channel port 114 of the fourth chromatography component, so that the liquid flows from the front end to the rear end of the fourth chromatography component, finishing the third chromatography, controlling the post-column central hole 130 of the post-column selection valve VIII to be communicated with the second post-column channel port 132 in the graph 9, and finally flowing into the UV detection device 6 through the post-column five-way valve B connected with the UV detection device. The number of the chromatography modules connected in series in the embodiment of the present invention is not limited to three, and the communication manner of three chromatography columns 12 connected in series is not limited to the one in fig. 9. The sample enters the mth chromatography component through the front column selection valve, is communicated with the fifth column front channel port 115 of the front column selection valve of the m-1 chromatography component through the fifth column rear channel port 135 of the rear column selection valve of the component, enters the m-1 chromatography component, and enters the rear multi-way valve of the column through the corresponding rear column selection valve; the sample enters the jth chromatographic component from the front column selection valve, is communicated with the front column channel port 136 of the front column selection valve of the jth-2 chromatographic component through the front column channel port 116 of the front column selection valve of the rear column selection valve of the component, enters the jth-2 chromatographic component, and enters the rear multi-way valve of the column through the corresponding rear column selection valve; the sample can pass through two or more than two chromatographic assemblies in turn in the form of a peak-off path, and the path can be selected and arranged in a mode of subtracting 1 or 2 in turn according to the sequence of the chromatographic assemblies, so that the aim of diversification is fulfilled.

In one embodiment of the present invention, referring to fig. 10, when the washing pump 43 is used to wash the chromatography apparatus in the forward direction, the conducting state of each pre-column channel port in the pre-column selection valve and each post-column channel port in the post-column selection valve is controlled according to the software, so that the liquid is pumped into one or more chromatography modules by the washing pump 43 through the reversing valve 71 and the pre-column five-way valve b in sequence, and is washed, and the liquid flows from the front end to the rear end of the chromatography module and then flows into the waste liquid port in a gathering manner through the post-column five-way valve a connected with the waste liquid port.

In an embodiment of the present invention, the reversing valve 71 is controlled to communicate the cleaning pump 43 with the post-column multi-way valve C, the cleaning solution is controlled to flow through the post-column multi-way valve C and a predetermined post-column selection valve from the rear end of the chromatographic column 12 to the front end direction through the chromatographic column under the action of the cleaning pump 43, and reaches the back pressure valve 72 through the corresponding post-column selection valve and the pre-column multi-way valve b, when the system pressure exceeds the critical pressure required for the conduction of the back pressure valve 72, the back pressure valve is conducted, and at this time, the cleaning solution passes through the back pressure valve 72 and enters the second waste liquid port 52; the above path process of the cleaning solution is also applicable to the condition of entering a plurality of groups of chromatographic assemblies, and is convenient for cleaning off the off-peak passages. Referring specifically to fig. 11, when the chromatographic apparatus is reversely cleaned by the cleaning pump 43, the software controls the conducting state of each front column port of the front column selection valve and each rear column port of the rear column selection valve, the software controls the reversing valve 71 to reverse, at this time, the direct path between the front six-way valve b and the cleaning pump 43 is disconnected, the liquid is reversed by the cleaning pump 43 through the reversing valve 71 and enters the rear five-way valve C connected with the reversing valve 71, and then is pumped into the rear three-way port 133 of one or more chromatographic assemblies, the liquid flows from the rear end to the front end of the chromatographic assembly and then reaches the front six-way valve, at this time, because the direct paths between the front six-way valves (i to iv) and the cleaning pump 43 are disconnected, the front six-way valve b is in a closed state, and because the front six-way valve b is also connected with the second waste liquid port 52 through the back pressure valve 72, therefore, when the pressure in the six-way valve b before the column exceeds the pre-pressure value required by the conduction of the back pressure valve 72, the action of discharging the liquid to the waste liquid port II through the back pressure valve 72 is realized, and the reverse cleaning of the chromatographic column, the corresponding valve and the pipeline by the cleaning pump 43 is realized.

When the method is applied, when a plurality of chromatographic columns are required to be loaded and eluted simultaneously, the serial arrangement sequence of the chromatographic columns can be selected randomly; the sample loading, elution and cleaning are respectively carried out by a sample pump, a system pump and a cleaning pump, and the one-way flushing and the reverse flushing of each chromatographic column can be simultaneously realized; only one set of UV detection devices is required; the number of the valves is small, the control logic relation is simple, the stability is high, and errors are not prone to occurring.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.