阅读说明：本技术 脱氨引起的序列错误的纠正 (Correction of sequence errors caused by deamination ) 是由 马尔辛·西科拉 安德鲁·肯尼迪 阿里尔·海莫维奇 达里娅·丘多瓦 史蒂芬·费尔克拉夫 于 2018-11-02 设计创作，主要内容包括：对核酸测序可以鉴定与疾病的存在、易感性或预后相关的变异。然而,这样的信息的价值可以因测序过程(包括制备用于测序的核酸)引入的错误或测序过程(包括制备用于测序的核酸)之前引入的错误而受损。使样品中核酸上的单链突出端(overhang)平末端化可引入脱氨引起的测序错误。本公开内容提供了鉴定和纠正这样的脱氨引起的测序错误并且将它们与真实序列变异区分开的方法。(Sequencing nucleic acids can identify variations that are associated with the presence, susceptibility or prognosis of a disease. However, the value of such information can be compromised by errors introduced by or prior to the sequencing process (including preparing the nucleic acid for sequencing). Blunting single-stranded overhangs (overhang) on nucleic acids in a sample can introduce sequencing errors caused by deamination. The present disclosure provides methods to identify and correct such deamination-induced sequencing errors and to distinguish them from true sequence variations.)