阅读说明：本技术 用于制备核酸文库的方法、组合物和试剂盒 (Methods, compositions and kits for preparing nucleic acid libraries ) 是由 富国良 T·邓威尔 于 2018-04-17 设计创作，主要内容包括：本发明涉及用于延伸多核苷酸的方法、组合物和试剂盒以及用于制备多核苷酸的测序文库的方法、组合物和试剂盒，所述方法、组合物和试剂盒涉及在衔接子模板寡核苷酸上生成经修饰的靶多核苷酸以及标记靶序列的一条链或两条链。测序文库适用于大规模平行测序，并且包括多个双链核酸分子。(The present invention relates to methods, compositions and kits for extending polynucleotides and methods, compositions and kits for preparing sequencing libraries of polynucleotides, which methods, compositions and kits involve generating modified target polynucleotides on adaptor template oligonucleotides and labeling one or both strands of the target sequence. The sequencing library is suitable for massively parallel sequencing and comprises a plurality of double-stranded nucleic acid molecules.)