阅读说明：本技术 一种用于检测膀胱癌的标志物及膀胱癌检测试剂盒 (Marker for detecting bladder cancer and bladder cancer detection kit ) 是由 王帆 李霞玲 于 2019-09-09 设计创作，主要内容包括：本发明属于基因检测技术领域,具体公开了一种用于检测膀胱癌的标志物及膀胱癌检测试剂盒。所述的检测膀胱癌的标志物具有SEQ ID NO：1所示的序列；所述的膀胱癌检测试剂盒是以SEQ ID NO：1所述的序列作为标志物进行膀胱癌检测；其包含检测SEQ ID NO：1的检测引物,所述SEQ ID NO：1的检测引物的序列如SEQ ID NO：2和SEQ ID NO：3所示。本发明首次以SEQ ID NO：1作为标志物进行膀胱癌检测,提供了一种新的检测途径；同时,以SEQ ID NO：1作为标志物检测膀胱癌具有较高的准确度。(The invention belongs to the technical field of gene detection, and particularly discloses a marker for detecting bladder cancer and a bladder cancer detection kit. The marker for detecting bladder cancer has the nucleotide sequence shown in SEQ ID NO: 1; the bladder cancer detection kit is a kit comprising the following components in parts by weight of SEQ ID NO: 1 as a marker for detecting bladder cancer; which comprises detecting the nucleotide sequence of SEQ ID NO: 1, the detection primer of SEQ ID NO: 1 is shown as SEQ ID NO: 2 and SEQ ID NO: 3, respectively. The invention is expressed by SEQ ID NO: 1 as a marker for detecting bladder cancer, provides a new detection way; meanwhile, the sequence shown as SEQ ID NO: 1 as a marker for detecting bladder cancer has high accuracy.)

1. A marker for detecting bladder cancer, having the sequence of SEQ ID NO: 1 is shown in

The sequence of (a); wherein SEQ ID NO: 1 is as follows: 5'-GCTAGACATGCTGCGCTACCATCGAAGACGATAGCTTATCT-3' are provided.

2. Use of the marker of claim 1 in the preparation of a bladder cancer detection kit.

3. The bladder cancer detection kit is characterized by comprising

SEQ ID NO: 1 as a marker for detecting bladder cancer.

4. The bladder cancer detection kit of claim 3, comprising a nucleic acid sequence that detects the amino acid sequence of SEQ ID NO: 1, the detection primer of SEQ ID NO: 1 is shown as SEQ ID NO: 2 and SEQ ID NO: 3 is shown in the specification;

SEQ ID NO: 2 is as follows: 5'-GCGCAGATGCTACCCTGCTAGATCAGT-3', respectively;

SEQ ID NO: 3 is as follows: 5'-GTGCGTTAATATCTCAGCGAT-3' are provided.

5. The bladder cancer detection kit according to claim 4, further comprising RT-qPCR specific pre-mix, ddH 2O.

6. The bladder cancer detection kit according to claim 5, wherein the amounts of the components in the kit are: 5-15 ul of special premix for RT-qPCR; SEQ ID NO: 2. SEQ ID NO: 3. the primers are respectively 0.8-1.2 ul; ddH₂O 3~6ul。

7. The bladder cancer detection kit according to claim 6, wherein the amounts of the components in the kit are: 13 ul of special premix solution for RT-qPCR; SEQ ID NO: 2. SEQ ID NO: 3. the primers are respectively 1 ul; ddH₂O 5ul。

8. The bladder cancer detection kit of claim 6, wherein the amino acid sequence of SEQ ID NO: 2 and SEQ ID NO: the concentrations of 3 are 600-900 nM respectively.

9. The bladder cancer detection kit of claim 6, wherein the amino acid sequence of SEQ ID NO: 2 and SEQ ID NO: the concentrations of 3 were 800nM, respectively.

Technical Field

The invention relates to the technical field of gene detection, in particular to a marker and an arm for detecting bladder cancer

Cystoma detection kit.

Background

Bladder cancer refers to malignant tumor occurring on the mucous membrane of bladder, is the most common malignant tumor of urinary system, and is one of ten common tumors of the whole body. In the screening of bladder cancer, the first difficulty lies in determining which gene mutations are related to bladder cancer, and further designing a primer by using the gene as a marker to detect the gene, thereby realizing the screening of bladder cancer. However, in the prior art, the research on the markers of bladder cancer is not deep enough, and the markers capable of being used for predicting bladder cancer are not many, so that more markers for predicting bladder cancer are urgently needed to be researched.

Disclosure of Invention

The invention aims to solve the primary technical problem of providing a bladder cancer detection kit. The invention discovers that the nucleotide sequence of SEQ ID NO: 1; thus, the sequence set forth in SEQ ID NO: 1 is a marker, and whether the blood of a person to be tested contains SEQ ID NO: 1 sequence can be used to determine whether a person to be tested has bladder cancer.

The technical problem to be solved by the invention is realized by the following technical scheme:

the invention provides a marker for detecting bladder cancer, which has the nucleotide sequence shown in SEQ ID NO: 1; wherein SEQ ID NO: 1 is as follows: 5'-GCTAGACATGCTGCGCTACCATCGAAGACGATAGCTTATCT-3', respectively;

preferably, the marker is used for preparing a bladder cancer detection kit.

The invention also provides a bladder cancer detection kit, which is a kit comprising the following components in parts by weight of SEQ ID NO: 1 as a marker for detecting bladder cancer.

Preferably, the amino acid sequence of SEQ ID NO: 1 is shown as SEQ ID NO: 2 and SEQ ID NO: 3 is shown in the specification;

SEQ ID NO: 2 is as follows: 5'-GCGCAGATGCTACCCTGCTAGATCAGT-3', respectively;

SEQ ID NO: 3 is as follows: 5'-GTGCGTTAATATCTCAGCGAT-3' are provided.

Preferably, the bladder cancer detection kit further comprises RT-qPCR special premix and ddH 2O.

The special premix for RT-qPCR adopts HiScript II Q RT Supermix for qPCR.

Further preferably, the amounts of the components in the kit are: 5-15 ul of special premix for RT-qPCR; SEQ ID NO: 2. SEQ ID NO: 3. the primers are respectively 0.8-1.2 ul; ddH₂O 3~6ul。

Most preferably, the amounts of the components of the kit are: 13 ul of special premix solution for RT-qPCR; SEQ ID NO: 2. SEQ ID NO: 3. the primers are respectively 1 ul; ddH₂O 5 ul。

Further preferably, SEQ ID NO: 2 and SEQ ID NO: the concentrations of 3 are 600-900 nM respectively.

Most preferably, SEQ ID NO: 2 and SEQ ID NO: the concentrations of 3 were 800nM, respectively.

Has the advantages that: the invention is expressed by SEQ ID NO: 1 as a marker for bladder cancer detection, and the prior art does not provide a marker of SEQ ID NO: 1 is associated with bladder cancer; the invention provides a new detection way for detecting the bladder cancer and enriches the detection products of the bladder cancer. Studies have shown that the sequence shown in SEQ ID NO: 1 has higher accuracy as a marker; in addition, the kit provided by the invention can realize the effect of the detection of the nucleotide sequence shown by SEQ ID NO: 1, and further experiments show that the expression of SEQ ID NO: 1 is expressed in an amount greater than 1.6 x10^-4umol/L, therefore, the accuracy of the bladder cancer detection can be further improved by taking the quantitative data as a reference standard.

Detailed Description

The present invention is further explained below with reference to specific examples, which are not intended to limit the present invention in any way.