阅读说明：本技术 一种香蕉皮功能性色素的提取方法 (Method for extracting functional pigment from banana peel ) 是由 谭丽燕 于 2019-11-29 设计创作，主要内容包括：本发明涉及一种香蕉皮功能性色素的提取方法,属于农产品加工领域；具体包括(1)预处理：挑选新鲜、无病害香蕉皮,进行清洗；(2)护色：将预处理后的香蕉皮置于护色液中浸泡护色；(3)干燥：将护色后的香蕉皮真空干燥箱中烘干；(4)粉碎：将干燥的香蕉皮粉碎；(5)提取：将香蕉皮粉末加入浸提溶剂丙酮中,提取一定时间后,取上清液即为粗提液；(6)纯化：用离子交换凝胶柱对粗提液进行分离纯化得到纯化色素溶液；(7)浓缩：对纯化色素溶液进行真空浓缩；(8)干燥：对浓缩后的色素进行冷冻干燥,即可得到成品；该方法提取率高,提取的香蕉皮类胡萝卜素质量好,对金黄色葡萄球菌及大肠杆菌有明显抑制作用。(The invention relates to a method for extracting functional pigment from banana peel, belonging to the field of agricultural product processing; the method specifically comprises the following steps of (1) preprocessing: selecting fresh and disease-free banana peels, and cleaning; (2) color protection: placing the pretreated banana peel in a color protection solution for soaking and color protection; (3) and (3) drying: drying the color-protected banana peel in a vacuum drying oven; (4) crushing: pulverizing dried banana peel; (5) extraction: adding banana peel powder into an extraction solvent acetone, extracting for a certain time, and taking supernatant fluid to obtain a crude extract; (6) and (3) purification: separating and purifying the crude extract by using an ion exchange gel column to obtain a purified pigment solution; (7) concentration: concentrating the purified pigment solution in vacuum; (8) and (3) drying: freeze-drying the concentrated pigment to obtain a finished product; the method has high extraction rate, and the extracted banana peel carotenoid has good quality and has obvious inhibiting effect on Staphylococcus aureus and Escherichia coli.)

1. A method for extracting functional pigment from banana peel is characterized by comprising the following steps: the method comprises the following steps:

(1) pretreatment: selecting fresh and disease-free banana peels, and cleaning;

(2) color protection: soaking the pretreated banana peel in a color protection solution for 5min for color protection;

(3) and (3) drying: drying the color-protected banana peel in a vacuum drying oven at 50 ℃;

(4) crushing: crushing the dried banana peel by a crusher to obtain banana peel powder;

(5) extraction: adding banana peel powder into an extraction solvent acetone according to a specific material-liquid ratio, extracting for a certain time at a specific temperature, centrifuging for 5min at a rotating speed of 4000 r/min, and taking supernatant as a crude extract;

(6) and (3) purification: separating and purifying the crude extract by using an ion exchange gel column to obtain a purified pigment solution;

(7) concentration: concentrating the purified pigment solution in vacuum;

(8) and (3) drying: and (4) freeze-drying the concentrated pigment to obtain a finished product.

2. The method for extracting functional pigment from banana peel as claimed in claim 1, wherein the method comprises the following steps: the color protection liquid in the step (2) is a mixed liquid of 0.4% of citric acid and 0.1% of ascorbic acid.

3. The method for extracting functional pigment from banana peel as claimed in claim 1, wherein the method comprises the following steps: the crushing degree in the step (4) is 40 meshes.

4. The method for extracting functional pigment from banana peel as claimed in claim 1, wherein the method comprises the following steps: the specific process parameters in the extraction in the step (5) are that the material-liquid ratio is 1: 10-1: 30, the temperature is 25-55 ℃, and the extraction time is 60-100 min.

5. The method for extracting functional pigment from banana peel as claimed in claim 1, wherein the method comprises the following steps: the drying in the step (8) is freeze drying.

Technical Field

The invention relates to a method for extracting functional pigments from banana peels, and belongs to the field of agricultural product processing.

Background

Bananas are the fruits with the largest production and sale quantity in the world, and are rich in various nutrient substances and deeply popular with people. The banana peel accounts for 35-41% of the total fruit mass, contains a plurality of bioactive components according to introduction, but is directly discarded by eaters generally, so that resources are wasted, and the environment is polluted. At present, there are reports of extracting bioactive substances such as polysaccharides and dietary fibers from banana peels, but there is no report of extracting functional pigments such as carotenoids.

The invention optimizes the extraction method of the banana peel carotenoid through experiments, preliminarily examines certain biological activities of the extracting solution, develops the extraction method of the banana peel functional pigment, develops the banana peel functional pigment with obvious inhibiting effect on staphylococcus aureus and escherichia coli, and aims to provide test basis for comprehensive utilization of banana peel and development and application of the carotenoid.

Disclosure of Invention

The invention aims to develop an extraction method of banana peel functional pigment, the extraction rate is high, the product quality is good, and the banana peel carotenoid has obvious inhibition effect on staphylococcus aureus and escherichia coli.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method for extracting functional pigment from banana peel comprises the following steps:

(1) pretreatment: selecting fresh and disease-free banana peels, and cleaning;

(2) color protection: soaking the pretreated banana peel in 0.4% citric acid and 0.1% ascorbic acid color protection solution for 5min for color protection;

(3) and (3) drying: drying the color-protected banana peel in a vacuum drying oven at 50 ℃;

(4) crushing: crushing the dried banana peel by a crusher, and sieving by a 40-mesh sieve to obtain banana peel powder;

(5) extraction: adding banana peel powder into an extraction solvent acetone according to a specific material-liquid ratio, extracting for a certain time at a specific temperature, centrifuging for 5min at a rotating speed of 4000 r/min, and taking supernatant as a crude extract;

(6) and (3) purification: separating and purifying the crude extract by using an ion exchange gel column to obtain a purified pigment solution;

(7) concentration: concentrating the purified pigment solution in vacuum;

(8) and (3) drying: and (4) freeze-drying the concentrated pigment to obtain a finished product.

The specific process parameters in the extraction in the step (5) are that the material-liquid ratio is 1: 10-1: 30, the temperature is 25-55 ℃, and the extraction time is 60-100 min.

The invention has the following advantages:

the method has high extraction rate and good product quality, and has obvious inhibiting effect on staphylococcus aureus and escherichia coli.

Detailed Description

The present invention is further illustrated in detail by the following examples, which are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.