阅读说明：本技术 一种l-乳酸盐检测试剂盒 (L-lactate detection kit ) 是由 刘鹤 刘希 于 2019-09-20 设计创作，主要内容包括：本发明涉及了一种L-乳酸盐检测试剂盒。本申请的试剂盒包括20mmol/L至300mmol/L,pH＝6至9的缓冲液、乳酸氧化酶0.1至2.0ku/L、过氧化物酶0.1ku/L至10ku/L、4-氨基安替比林0.1至30mmol/L、色原0.1mmol/L至30mmol/L、防腐剂0.01％-5％、非离子表面活性剂、两性表面活性剂和蛋白类稳定剂。本申请的试剂盒具有抗Vc干扰,开盖存放1个月不用定标,有效期内试剂外观变化不明显,非干粉液体试剂等优点。(The invention relates to a L-lactate detection kit, which comprises a buffer solution with the pH value of between 20 and 300mmol/L and between 6 and 9, lactate oxidase 0.1 to 2.0ku/L, peroxidase 0.1 to 10ku/L, 4-aminoantipyrine 0.1 to 30mmol/L, chromogen 0.1 to 30mmol/L, preservative 0.01 to 5 percent, nonionic surfactant, amphoteric surfactant and protein stabilizer.)

1, L-lactate test kits comprising:

wherein the protein stabilizing agent is selected from or the combination of the following:

glutamate, glycinate, bovine serum albumin, ovalbumin, polylysine and skimmed milk powder.

2. The L-lactate test kit according to claim 1, wherein the nonionic surfactant is selected from selected from Triton X-100, lauryl alcohol, sodium cholate, n-octyl glucoside, polyethylene glycol monododecyl ether, and polyoxyethylene alkyl ether.

3. The L-lactate test kit according to claim 1, wherein the amphoteric surfactant is selected from selected from alkyldimethylamine acetate betaine, alkylhydroxysultaine, lauramidopropyl betaine, and alkylcarboxymethylimidazole betaine.

4. The L-lactate detection kit according to claim 1, further comprising ascorbic acid oxidase in an amount of 1 to 100 ku/L.

5. The L-lactate detection kit according to claim 1, wherein the buffer is selected from or a combination thereof, Tris buffer, GOOD' S buffer, Pipes buffer, phosphate buffer, citric acid-sodium citrate buffer, sodium carbonate-sodium bicarbonate buffer.

6. The L-lactate detection kit according to claim 1, wherein the preservative is selected from of sodium azide, lithium azide, PC300, alkyl glycosides, and methylisothiazolinone, or a combination thereof.

7. The L-lactate detection kit according to claim 1, wherein the chromogen is selected from or a combination of phenol, sodium 3, 5-dichloro-2-hydroxybenzenesulfonate, 2-amino-4-hydroxybenzenesulfonic acid, and N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt.

An L-lactate test kit comprising:

an L-lactate test kit comprising:

the BSA is selected from: a fifth component of bovine serum albumin, bovine serum albumin (without essential fatty acid grade), bovine serum albumin (without protease grade), bovine serum albumin (cell culture grade), gold-standard grade bovine serum albumin, cross-linked bovine serum albumin;

preferably, the BSA is a fifth component of bovine serum albumin.

10. The L-lactate detection kit according to of any one of claims 1-9, which is in liquid form, not in dry powder form.