rotary streaking inoculation method

文档序号:1704282 发布日期:2019-12-13 浏览:44次 中文

阅读说明:本技术 一种旋转划线接种方法 (rotary streaking inoculation method ) 是由 姜世民 余康林 于 2019-09-18 设计创作,主要内容包括:本发明涉及一种旋转划线接种方法,该划线接种方法,包括以下步骤,a、盛有培养基的培养皿旋转;b、蘸有样本液的接种器沿培养皿半径方向水平运动,在培养皿上的培养基上进行接种划线;c、接种器绕轴线转动。本发明的旋转划线接种方法,蘸有样本液的接种器沿培养皿半径方向水平运动,在培养皿上的培养基上进行接种划线;接种器绕轴线转动。实现螺旋划线接种,接种更为均匀。(The invention relates to a rotary streak inoculation method, which comprises the following steps that a, a culture dish containing a culture medium rotates; b. horizontally moving the inoculator dipped with the sample liquid along the radius direction of the culture dish, and carrying out inoculation and streaking on a culture medium on the culture dish; c. the inoculator is rotated about an axis. According to the rotary streak inoculation method, an inoculator dipped with sample liquid horizontally moves along the radius direction of a culture dish, and inoculation streaking is carried out on a culture medium on the culture dish; the inoculator is rotated about an axis. The spiral streak inoculation is realized, and the inoculation is more uniform.)

1. A method of rotational streaking inoculation, comprising: comprises the following steps of (a) carrying out,

a. rotating the culture dish containing the culture medium;

b. Horizontally moving the inoculator dipped with the sample liquid along the radius direction of the culture dish, and carrying out inoculation and streaking on a culture medium on the culture dish;

c. The inoculator is rotated about an axis.

2. The rotational streaking inoculation method of claim 1 wherein: the culture dish is rotated according to a set speed.

3. A method of rotational streaking as in claim 1 or claim 2 wherein: the inoculator pivoting is performed simultaneously with the rotation of the culture dish.

4. The rotational streaking inoculation method of claim 1 wherein: the inoculator moves horizontally along the radius direction of the culture dish.

5. the rotational streaking inoculation method of claim 1 wherein: the end of the inoculator is dipped with the sample liquid, and then inoculation streaking is carried out on a culture dish.

Technical Field

the invention relates to a culture dish coating device, in particular to a device for dynamically streaking a rotary inoculator on a rotary culture dish.

Background

In hospital clinical and medical research trials, it is often necessary to spread biological samples or prepared bacterial suspensions from patients onto petri dishes. The current general coating method: the coating process is completed by using a traditional inoculating loop and adopting a manual method.

however, due to the existing manual streaking inoculation mode, the randomness is high in the streaking process, and the liquid sample is difficult to be uniformly coated on the culture dish according to the assumption of a tester, so that the detection result is influenced.

Disclosure of Invention

In order to solve the problems, the invention provides a rotary streak inoculation method which is reasonable in structure and convenient to use.

The technical scheme adopted by the invention is as follows: a method of rotational streaking inoculation, comprising: comprises the following steps of (a) carrying out,

a. Rotating the culture dish containing the culture medium;

b. The inoculator dipped with the sample liquid horizontally moves along the radius direction of the culture dish, or from the circle center to the circle center;

c. the inoculator was rotated about the axis and self-transferred 360 deg. from start to end of the scoring process.

the three movements start and end simultaneously.

preferably, the culture dish rotates according to a set speed. Meanwhile, the inoculator is automatically transferred along the shaft and moves along the radius direction of the culture dish at the same time.

Preferably, the tail end of the inoculator is dipped in the sample liquid, then contacts with the culture dish, moves along the radius direction of the culture dish while automatically transmitting around the shaft, and carries out dynamic inoculation and streaking.

The beneficial effects obtained by the invention are as follows: according to the rotary streak inoculation method, an inoculator dipped with sample liquid horizontally moves along the radius direction of a culture dish, and inoculation and streak are carried out on the rotary culture dish; simultaneously the inoculator was rotated about the axis, i.e. the whole of the sample carried on the inoculator was streaked 360 ° a week onto the culture medium in a petri dish. The spiral streak inoculation is realized, and the inoculation is more uniform and controllable.

drawings

FIG. 1 is a prior art inoculation streaking method;

FIG. 2 is a streaking inoculation mode of the present invention;

FIG. 3 is a schematic diagram of detection by drug sensitive test paper;

Reference numerals: 1. a culture dish; 2. an inoculator; 3. drug sensitive test paper.

Detailed Description

The invention will be further described with reference to the following drawings and specific embodiments.

As shown in FIGS. 1-3, a spin-streaking method of the present invention is characterized by: comprises the following steps of (a) carrying out,

a. The culture dish 1 containing the culture medium rotates according to a set speed;

b. Horizontally moving the inoculator 2 dipped with the sample liquid along the radius direction of the culture dish, and carrying out inoculation and streaking on the culture medium on the culture dish 1;

c. The inoculator 2 rotates around an axis, and the inoculator 2 automatically transfers around the axis while moving along the radius of the culture dish, so that all samples on the inoculator can be conveniently coated on the culture dish in 360 degrees.

By the streaking inoculation method, the inoculator 2 can perform spiral streaking on the culture medium, and streaking inoculation is ensured to be more uniform.

In this example, the sample solution was dipped into the end of the inoculator 2 by a cotton ball or sponge, and then streaked.

the foregoing shows and describes the general principles and principal structural features of the present invention. The present invention is not limited to the above examples, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

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