阅读说明：本技术 一种含植物干细胞的抑菌组合物、养护抑菌液及其制备方法 (Plant stem cell-containing antibacterial composition, maintenance antibacterial liquid and preparation method thereof ) 是由 张芳 于 2021-09-10 设计创作，主要内容包括：本申请涉及医疗卫生领域,具体公开了一种含植物干细胞的抑菌组合物、养护抑菌液及其制备方法,该抑菌组合物由包含以下重量份的原料制得：狄伟草提取物、苦参碱、蒲公英、卡琪花蒂玛、份芦荟、青黛、红花、当归、燕麦提取物、洋甘菊提取物、山茱萸、乙酰基六肽-8和苹果干细胞冻干粉；养护抑菌液包括所述的抑菌组合物和保湿剂,水补足；养护抑菌液的制备方法包括以下步骤：浸膏制备；基液制备；养护液制备：将浸膏溶解与基液混合,加入狄伟草提取物、保湿剂和乙酰基六肽-8,得到滤液；取部分水溶解苹果干细胞冻干粉,然后加入滤液中,搅拌,加入剩余水,调pH值,得养护抑菌液。本申请具有提供抑菌效果更好的抑菌产品特点。(The application relates to the field of medical health, and particularly discloses a plant stem cell-containing bacteriostatic composition, a maintenance bacteriostatic liquid and a preparation method thereof, wherein the bacteriostatic composition is prepared from the following raw materials in parts by weight: the composition comprises a mixture of a divaricate saposhnikovia root extract, matrine, dandelion, caramel calyx ma, aloe, indigo naturalis, safflower, angelica sinensis, an oat extract, a chamomile extract, dogwood, acetyl hexapeptide-8 and apple stem cell freeze-dried powder; the maintenance antibacterial liquid comprises the antibacterial composition and a humectant, and is supplemented with water; the preparation method of the maintenance bacteriostat comprises the following steps: preparing an extract; preparing base liquid; preparing a curing liquid: dissolving the extract, mixing with the base solution, and adding the extract of DIWEICAO, humectant and acetyl hexapeptide-8 to obtain filtrate; and (3) dissolving part of water in the apple stem cell freeze-dried powder, then adding the apple stem cell freeze-dried powder into the filtrate, stirring, adding the rest water, and adjusting the pH value to obtain the maintenance antibacterial solution. The application has the characteristic of providing a bacteriostatic product with better bacteriostatic effect.)

1. The bacteriostatic composition containing the plant stem cells is characterized by being prepared from the following raw materials in parts by weight: 3-8 parts of a peganum davidii extract, 0.2-0.5 part of matrine, 3-7 parts of dandelion, 3-8 parts of caramel calyx, 3-7 parts of aloe, 3-8 parts of indigo naturalis, 3-8 parts of safflower, 3-7 parts of angelica, 0.5-1.5 parts of an oat extract, 1-4 parts of a chamomile extract, 3-8 parts of dogwood, 1-3 parts of acetyl hexapeptide-8 and 0.5-1 part of plant dry cell freeze-dried powder, wherein the plant dry cell freeze-dried powder is apple dry cell freeze-dried powder.

2. The bacteriostatic composition containing plant stem cells according to claim 1, wherein: the antibacterial composition also comprises 3-8 parts of pearl powder and 1-4 parts of tremella polysaccharide.

3. A nourishing and protecting bacteriostatic liquid is characterized in that: the bacteriostatic composition as claimed in claim 1, 6-12 parts of humectant and water in parts by weight, wherein the water is added to make up to 100 parts.

4. The nourishing and bacteriostasis liquid of claim 3, wherein the moisturizer is prepared from the following raw materials in a mass ratio of 1: (0.8-1.2) glycerin and sodium hyaluronate.

5. The bacteriostatic nourishing and protecting liquid according to claim 3, wherein: the antibacterial composition also comprises 3-8 parts of pearl powder and 1-4 parts of tremella polysaccharide.

6. The bacteriostatic nourishing and protecting liquid according to claim 3, wherein: the maintenance antibacterial liquid also comprises 1-2 parts of ethylparaben, 6-10 parts of sodium dodecyl sulfate and 3-5 parts of tween-80.

7. A method of preparing the curing bacteriostat as in claim 3, wherein the method comprises the following steps: the method comprises the following steps:

preparing an extract: decocting herba Taraxaci, Carthami flos, Aloe, radix Angelicae sinensis, indigo naturalis, Corni fructus and caramel calyx Ma, and concentrating to obtain extract;

preparing a base liquid: dissolving matrine in ethanol to obtain base solution;

preparing a curing liquid: dissolving the extract, mixing with the base solution, and adding the extract of DIWEICAO, the extract of Matricaria Chamomilla, the extract of Avena sativa, the humectant and acetyl hexapeptide-8 to obtain a filtrate;

dissolving part of water in the apple stem cell freeze-dried powder to obtain a stem cell freeze-dried powder water solution;

and adding the stem cell freeze-dried powder aqueous solution into the filtrate, stirring, adding the rest water, and adjusting the pH value to obtain the maintenance antibacterial solution.

8. The method for preparing a nourishing bacteriostatic fluid according to claim 7, which is characterized in that:

the maintenance antibacterial liquid also comprises 3-8 parts of pearl powder and 1-4 parts of tremella polysaccharide, wherein the pearl powder is added with the dandelion in the preparation step of the extract; the Tremella polysaccharide is added with the extract of herba Sabinae Laevigatae in the preparation step of the tonic solution.

9. The method for preparing a nourishing and bacteriostatic liquid according to claim 7 or 8, which is characterized in that: the maintenance antibacterial liquid also comprises 1-2 parts of ethylparaben, 6-10 parts of sodium dodecyl sulfate and 3-5 parts of tween-80;

adding ethylparaben and tween-80 in the base solution preparation step;

adding sodium dodecyl sulfate into the stem cell lyophilized powder water solution in the step of preparing the maintenance solution, and then adding.

10. The method for preparing a nourishing bacteriostatic fluid according to claim 7, which is characterized in that: the concrete operation of the extract preparation is as follows: decocting the above materials in water twice, filtering, removing residue, mixing filtrates, and heating and concentrating to obtain extract with relative density of 1.1-1.2 at 60 deg.C; and then adding ethanol into the extract to enable the alcohol content in the extract to reach 75%, stirring, standing for 23-24h, taking supernate, and recovering ethanol until no alcohol smell exists, thus obtaining the extract.

Technical Field

The application relates to the technical field of medical treatment and health, in particular to a bacteriostatic composition containing plant stem cells, a maintenance bacteriostatic solution and a preparation method thereof.

Background

Gynecological inflammation is a common disease of women, and is caused by that many people lack due knowledge of gynecological diseases, lack of health care of bodies and various diseases caused by bad living habits, and finally, a large number of toxins and bacteria are hidden in the vagina to cause the internal environment of the vagina to be disordered. The common gynecological inflammations include female vulvitis, vaginitis, cervicitis and the like. Clinically, gynecological inflammation mainly shows that vaginal secretion is increased and is accompanied by symptoms such as peculiar smell, pruritus vulvae, leukorrhagia, burning pain and the like, and great inconvenience is brought to normal life and work. Therefore, the maintenance bacteriostat liquid has important significance for preventing and relieving gynecological inflammation.

At present, the main medicines for gynecological inflammation in China comprise antibiotics, western medicines and traditional Chinese medicines, the antibiotics are easy to generate drug resistance, once drug-resistant strains are generated in a human body, the state of an illness cannot be effectively controlled even if the dosage of the medicines is continuously increased, the antibiotics are easy to damage the balance of vaginal flora, and the recurrence rate of the inflammation after use is high; while western medicines mainly utilize chemical components for sterilization and treatment of symptoms, although the effects are quick, the toxic and side effects such as irritation and dependence are relatively large, so that Chinese herbal medicines are widely applied to bacteriostatic products at present, such as common cnidium fruit, lightyellow sophora root, golden cypress and the like which have the effects of clearing away heat and toxic materials and relieving itching, but the bacteriostatic effect of the current vaginal bacteriostatic products is not ideal.

Disclosure of Invention

In order to provide a bacteriostatic maintenance product with better bacteriostatic effect, the application provides a bacteriostatic composition containing plant stem cells, a maintenance bacteriostatic solution and a preparation method thereof.

In a first aspect, the bacteriostatic composition containing plant stem cells provided by the application adopts the following technical scheme:

a bacteriostatic composition containing plant stem cells is prepared from the following raw materials in parts by weight: 3-8 parts of a peganum davidii extract, 0.2-0.5 part of matrine, 3-7 parts of dandelion, 3-8 parts of caramel calyx, 3-7 parts of aloe, 3-8 parts of indigo naturalis, 3-8 parts of safflower, 3-7 parts of angelica, 0.5-1.5 parts of an oat extract, 1-4 parts of a chamomile extract, 3-8 parts of dogwood, 1-3 parts of acetyl hexapeptide-8 and 0.5-1 part of plant dry cell freeze-dried powder.

By adopting the technical scheme, the matrine has the sterilization and inflammation diminishing effects, has different inhibiting effects on the fungal infection in the vagina, also has the effects of clearing heat, promoting urination and removing dampness, and the dandelion has the spectral antibacterial effect, has certain effects on gram-positive bacteria, gram-negative bacteria and fungal bacteriostasis, does not generate drug resistance and has excellent antibacterial effect.

The applicant finds that the addition of the diewei grass extract and the acetyl hexapeptide-8 in the antibacterial composition can significantly improve the antibacterial effect of the antibacterial composition, probably because the formation of gynecological inflammation is a comprehensive process, and researches show that women only retain basal layer cells and accessory basal layer cells which are malnutritional and have easily fallen villi due to the reduction of estrogen, epithelial atrophy and disappearance of squamous cell layers after menopause, and the vaginal mucosa is easily infected by bacteria and toxic metabolites and easily forms a small laceration to cause infection.

Probably, the dievicoside molecules in the dievica extract are very close to the molecules of natural lutein of a human body, the dievica extract has certain influence on internal hormones of the human body and can improve the body function, the addition of the acetyl hexapeptide-8 can protect epidermal mucosa from being invaded by bacteria, the applicant finds that the breeding of related bacteria in the vagina can be effectively inhibited by the compounding of the dievica extract and the acetyl hexapeptide-8, and the obtained antibacterial composition has better antibacterial effect. The addition of the apple stem cell freeze-dried powder further improves the bacteriostatic action of the bacteriostatic composition, and finally obtains a maintenance bacteriostatic product with better bacteriostatic effect, and has better effect on the treatment and prevention of gynecological inflammation.

The dogwood and the aloe have certain bacteriostatic action, and the caramel calyx-shaped massa is warm in nature, and can promote blood circulation to remove blood stasis, regulate menstruation, relieve pain and reduce leucorrhea; carthami flos has effects of promoting blood circulation, dredging channels, dispelling blood stasis, and relieving pain; the angelica has the functions of enriching and activating blood, regulating menstruation and relieving pain; indigo naturalis has effects of cooling and relieving fever, cooling blood and removing speckle, Corni fructus has effects of invigorating liver and kidney, astringing and inducing astringency, and also has certain antibacterial effect, and Aloe has certain effects of diminishing inflammation, relieving itching and relieving pain. The oat extract has certain skin itch reducing and inflammation diminishing effects.

The substances in the application probably have the effects of promoting blood circulation, stimulating the menstrual flow and the like due to the substances such as angelica sinensis, safflower and the like, and the peganum davidii extract is used for regulating hormone, and antibacterial substances such as matrine and the like also have the effects of sterilizing and diminishing inflammation and the like. And through the action of substances for promoting blood circulation, regulating menstruation and the like, the gynecological inflammation probability is reduced radically, and the effects of regulating the gynecological inflammation root conditions to later-stage sterilization, repair and other paths are realized, so that the effects of maintaining and inhibiting bacteria are achieved.

Optionally, the bacteriostatic composition further comprises 3-8 parts of pearl powder and 1-4 parts of tremella polysaccharide.

By adopting the technical scheme, the pearl powder can enhance immunity, has the effects of sterilizing, promoting granulation and the like, the tremella polysaccharide can enhance cellular immunity and humoral immunity of a human body, and has excellent moisturizing performance, so that the skin has good smooth and moist feeling, the vaginal dryness is improved, and the maintenance and bacteriostasis effects of the maintenance bacteriostat are further enhanced.

In a second aspect, the application provides a nursing bacteriostatic solution, which adopts the following technical scheme:

a nourishing and protecting antibacterial liquid comprises the antibacterial composition, 6-15 parts of humectant and water which are complemented to 100 parts.

Through adopting above-mentioned technical scheme, the maintenance antibacterial liquid that makes in this application is used for vagina cleanness and maintenance, play excellent bacteriostatic action, not only have certain effect to gynaecology's inflammation such as vaginitis, still have certain repairing action to vagina mucosa epithelial cell layer, help vagina's natural defense function to maintain or resume, but also can regard as women's vaginal maintenance liquid, play clean maintenance efficiency, prevent bacterial growing, produce a plurality of routes such as later stage sterilization and restoration through gynaecology's inflammation and adjust to play a plurality of routes and act on, thereby play the antibacterial efficiency of maintenance.

Optionally, the humectant is selected from the following components in a mass ratio of 1: (0.8-1.2) glycerin and sodium hyaluronate.

By adopting the technical scheme, the humectant has a certain water locking effect on the maintenance antibacterial liquid, can also improve the vaginal dryness phenomenon, has a certain effect on improving vaginal lubrication, has small stimulation to human bodies, has emulsification promoting effect on glycerol, and can promote mutual solubility of the raw materials.

Optionally, the maintenance bacteriostatic liquid further comprises 1-2 parts of ethylparaben, 6-10 parts of sodium dodecyl sulfate and 3-5 parts of

Tween-80.

Through adopting above-mentioned technical scheme, the interpolation of ethylparaben plays the anticorrosive effect that disinfects to antibacterial protection liquid, and also play certain guard action to the vagina after using and receive bacterium and fungal infection, sodium dodecyl sulfate is as solubilizer and emulsifier, not only can increase the raw materials in the dissolution of aquatic and mutual dissolution, form the homogeneous liquid, and can also play and promote the osmosis, the effect of solubilizer and dispersant can also be played in the interpolation of tween-80, be favorable to the dispersion of raw materials in aqueous to dissolve, help forming the maintenance liquid of homogeneity, add sodium dodecyl sulfate and tween-80 in this application, promote the dispersion of each raw materials in aqueous to dissolve, and still have excellent antibacterial performance, prevent that too much of tween-80 addition from leading to the reduction of maintenance liquid antibacterial effect.

In a third aspect, the preparation method of the nourishing and bacteriostasis liquid provided by the application adopts the following technical scheme:

a preparation method of a nourishing and bacteriostasis liquid comprises the following steps:

preparing an extract: decocting herba Taraxaci, Carthami flos, Aloe, radix Angelicae sinensis, indigo naturalis, Corni fructus and caramel calyx Ma, and concentrating to obtain extract; preparing a base liquid: dissolving matrine in ethanol to obtain base solution;

preparing a curing liquid: dissolving the extract, mixing with the base solution, and adding the extract of DIWEICAO, the extract of Matricaria Chamomilla, the extract of Avena sativa, the humectant and acetyl hexapeptide-8 to obtain a filtrate;

dissolving part of water in the apple stem cell freeze-dried powder to obtain a stem cell freeze-dried powder water solution;

and adding the stem cell freeze-dried powder aqueous solution into the filtrate, stirring, adding the rest water, and adjusting the pH value to obtain the maintenance antibacterial solution.

By adopting the technical scheme, the antibacterial composition is prepared into liquid, so that the absorption is quicker, the absorption effect is better, and an excellent antibacterial effect is achieved.

Optionally, the maintenance antibacterial liquid further comprises 3-8 parts of pearl powder and 1-4 parts of tremella polysaccharide, wherein the pearl powder is added together with the dandelion in the preparation step of the extract; the Tremella polysaccharide is added with the extract of herba Sabinae Laevigatae in the preparation step of the tonic solution.

Optionally, the maintenance antibacterial liquid further comprises 1-2 parts of ethylparaben, 6-10 parts of sodium dodecyl sulfate and 3-5 parts of tween-80;

adding ethylparaben and tween-80 in the base solution preparation step;

adding sodium dodecyl sulfate into the stem cell lyophilized powder water solution in the step of preparing the maintenance solution, and then adding.

Optionally, the concrete operations for preparing the extract are as follows: decocting the above materials in water twice, filtering, removing residue, mixing filtrates, and heating and concentrating to obtain extract with relative density of 1.1-1.2 at 60 deg.C; and then adding ethanol into the extract to enable the alcohol content in the extract to reach 75%, stirring, standing for 23-24h, taking supernate, and recovering ethanol until no alcohol smell exists, thus obtaining the extract.

In summary, the present application has the following beneficial effects:

1. the maintenance antibacterial liquid prepared in the application is used for vagina cleaning and maintenance, plays an excellent antibacterial role, has a certain role in gynecological inflammation such as vaginitis, has a certain repairing role in vaginal mucosa epithelial cell layers, is beneficial to maintaining or recovering the natural defense function of vagina, can be used as a maintenance liquid for female vagina, plays a role in cleaning and maintenance, prevents bacteria from breeding, and plays a role in regulating multiple paths such as later sterilization and repair through the root conditions generated by gynecological inflammation, so that the antibacterial maintenance effect is played;

2. in the application, probably because the pegamun rivularis extract has a certain effect on hormone in a human body to improve the physical function, the addition of the acetyl hexapeptide-8 can protect epidermal mucosa from being invaded by bacteria, and the fact that the pegamun rivularis extract and the acetyl hexapeptide-8 are compounded for use can effectively inhibit the breeding of related bacteria in the vagina, so that the obtained antibacterial composition has a better antibacterial effect;

3. the pearl powder can enhance immunity, has the effects of sterilizing, promoting granulation and the like, the tremella polysaccharide can enhance the cellular immunity and humoral immunity of a human body, and has excellent moisturizing performance, so that the skin has good smooth and moist feeling, the vaginal dryness is improved, and the maintenance and bacteriostasis effects of the maintenance bacteriostat are further enhanced;

4. the moisturizing agent in the maintenance antibacterial liquid has a certain water locking effect on the maintenance antibacterial liquid, can improve the vaginal dryness phenomenon, has a certain effect on improving vaginal lubrication, and has small stimulation to human bodies;

5. the addition of glycerine, sodium dodecyl sulfate and tween-80 in this application maintenance bacteriostat can play the effect of solubilization, still plays the emulsification dispersion effect, helps the mutual solution between the raw materials and the dissolution dispersion in aqueous, finally forms even maintenance liquid.

Detailed Description

The present application is further described in detail with reference to the following examples, which are specifically illustrated by the following: the following examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer, and the starting materials used in the following examples are available from ordinary commercial sources unless otherwise specified.

The apple stem cell freeze-dried powder in the application can be prepared by adopting a conventional method in the field, and can also be obtained from the common market of the Xian Zealang biotechnology limited company.

The bacteriostatic composition provided by the application can be used for different formulations such as maintenance bacteriostatic liquid or maintenance bacteriostatic paste, and the maintenance bacteriostatic liquid formulation is taken as an example in the embodiment of the application for explanation.

Examples

Example 1

A preparation method of a nourishing and bacteriostasis liquid comprises the following steps:

preparing an extract: adding raw materials of 30g of dandelion, 30g of safflower, 30g of aloe, 30g of angelica, 30g of indigo naturalis, 30g of dogwood and 30g of caramel calyx Ma into water which is 8 times (by mass) the sum of the raw materials, decocting for 1.5h, filtering, and collecting filtrate; adding the filter residue into 6 times (by mass) of water, decocting for 1h, filtering, and collecting the filtrate; mixing the filtrates, concentrating to obtain extract with relative density of 1.1 at 60 deg.C, adding ethanol to make ethanol content in extract reach 75%, stirring, standing for 24 hr, collecting supernatant, and recovering ethanol until no ethanol smell exists to obtain extract;

preparing a base liquid: dissolving 2g of matrine and 10g of ethylparaben in 6 mass times of ethanol, adding 30g of tween-80, and stirring to obtain a base solution;

preparing a curing liquid: dissolving the extract in water with the mass ratio of 3, mixing with the base solution, and adding 30g of a peganum lab extract, 60g of a humectant, 10g of acetyl hexapeptide-8, 10g of a chamomile extract and 5g of an oat extract to obtain a filtrate, wherein the humectant comprises sodium hyaluronate and glycerol in a mass ratio of 1: 0.8;

dissolving 5g of apple stem cell freeze-dried powder in water with the mass multiple of 2 to obtain a stem cell freeze-dried powder aqueous solution;

adding the stem cell freeze-dried powder aqueous solution into the filtrate, stirring, then adding 60g of sodium dodecyl sulfate, stirring, adding water to supplement to 1000g, dropwise adding triethanolamine to adjust the pH value to 6.5, and uniformly stirring to obtain the maintenance antibacterial solution.

Example 2

A preparation method of a nourishing and bacteriostasis liquid comprises the following steps:

preparing an extract: adding 50g of dandelion, 50g of safflower, 50g of aloe, 50g of angelica, 50g of indigo naturalis, 50g of dogwood and 50g of caramel calyx Ma into water which is 8 times (by mass) the sum of the raw materials, decocting for 1.5h, filtering, and collecting filtrate; adding the filter residue into 6 times (by mass) of water, decocting for 1h, filtering, and collecting the filtrate; mixing the filtrates, concentrating to obtain extract with relative density of 1.1 at 60 deg.C, adding ethanol to make ethanol content in extract reach 75%, stirring, standing for 24 hr, collecting supernatant, and recovering ethanol until no ethanol smell exists to obtain extract;

preparing a base liquid: dissolving 3g of matrine and 15g of ethylparaben in 6 mass times of ethanol, adding 40g of tween-80, and stirring to obtain a base solution;

preparing a curing liquid: dissolving the extract in water with the mass ratio of 3, mixing with the base solution, and adding 50g of a peganum rivularis extract, 100g of a humectant, 20g of acetyl hexapeptide-8, 20g of a chamomile extract and 10g of an oat extract to obtain a filtrate, wherein the humectant comprises sodium hyaluronate and glycerol in a mass ratio of 1: 1;

dissolving 8g of apple stem cell freeze-dried powder in water with the mass multiple of 2 to obtain a stem cell freeze-dried powder aqueous solution;

adding the obtained stem cell freeze-dried powder aqueous solution into the filtrate, stirring, then adding 80g of sodium dodecyl sulfate, stirring, adding water to supplement to 1000g, dropwise adding triethanolamine to adjust the pH value to 7, and uniformly stirring to obtain the maintenance antibacterial solution.

Example 3

A preparation method of a nourishing and bacteriostasis liquid comprises the following steps:

preparing an extract: adding raw materials 70g of dandelion, 80g of safflower, 70g of aloe, 70g of angelica, 80g of indigo naturalis, 80g of dogwood and 80g of caramel calyx Ma into water which is 8 times (by mass) the sum of the raw materials, decocting for 1.5h, filtering, and collecting filtrate; adding the filter residue into 6 times (by mass) of water, decocting for 1h, filtering, and collecting the filtrate; mixing the filtrates, concentrating to obtain extract with relative density of 1.1 at 60 deg.C, adding ethanol to make ethanol content in extract reach 75%, stirring, standing for 24 hr, collecting supernatant, and recovering ethanol until no ethanol smell exists to obtain extract;

preparing a base liquid: dissolving 5g of matrine and 20g of ethylparaben in 7 mass times of ethanol, adding 50g of tween-80, and stirring to obtain a base solution;

preparing a curing liquid: dissolving the extract in 4 mass times of water, mixing with the obtained base solution, and adding 80g of a peganum lab extract, 120g of a humectant, 30g of acetyl hexapeptide-8, 40g of a chamomile extract and 15g of an oat extract to obtain a filtrate, wherein the humectant comprises sodium hyaluronate and glycerol in a mass ratio of 1: 1.2;

dissolving 10g of apple stem cell freeze-dried powder into water with the mass multiple of 3 to obtain a stem cell freeze-dried powder aqueous solution;

adding the stem cell freeze-dried powder aqueous solution into the filtrate, stirring, then adding 100g of sodium dodecyl sulfate, stirring, adding water to supplement to 1000g, dropwise adding triethanolamine to adjust the pH value to 7, and uniformly stirring to obtain the maintenance antibacterial solution.

Example 4

A preparation method of a nourishing bacteriostat is carried out according to the method in the embodiment 5, and the difference is that: in the step of preparing the extract, 30g of pearl powder is added while dandelion is added, the amount of water added in the first decoction is 8 times of the sum of the added raw materials, and the amount of water added in the second decoction is 6 times of the sum of the added raw materials; the rest of the procedure was the same as in example 2; in the preparation step of the nourishing liquid, 10g of tremella polysaccharide is added while the extract of the Chinese lizardtail herb is added.

Example 5

A preparation method of a nourishing bacteriostat is carried out according to the method in the embodiment 5, and the difference is that: in the step of preparing the extract, 50g of pearl powder is added while dandelion is added, the amount of water added in the first decoction is 8 times of the sum of the added raw materials, and the amount of water added in the second decoction is 6 times of the sum of the added raw materials; the rest of the procedure was the same as in example 2; in the preparation step of the nourishing liquid, 20g of tremella polysaccharide is added while the extract of the Chinese lizardtail herb is added.

Example 6

A preparation method of a nourishing bacteriostat is carried out according to the method in the embodiment 5, and the difference is that: in the step of preparing the extract, 80g of pearl powder is added while dandelion is added, the amount of water added in the first decoction is 8 times of the sum of the added raw materials, and the amount of water added in the second decoction is 6 times of the sum of the added raw materials; the rest of the procedure was the same as in example 2; in the preparation step of the nourishing liquid, 40g of tremella polysaccharide is added while the extract of the Chinese lizardtail herb is added.

Example 7

A preparation method of a nourishing and bacteriostasis liquid is carried out according to the method in the embodiment 5, except that Tween-80 is equivalently replaced by sodium dodecyl sulfate.

Comparative example

Comparative example 1

A preparation method of a nourishing and bacteriostasis liquid is carried out according to the method in the embodiment 2, and the difference is that: in the preparation step of the tonic solution, acetyl hexapeptide-8 and herba Saussureae Dilutae extract are not added.

Comparative example 2

A preparation method of a nourishing and bacteriostasis liquid is carried out according to the method in the embodiment 2, and the difference is that: in the preparation step of the nourishing liquid, acetyl hexapeptide-8 is substituted for the extract of the soot tree in equal amount.

Comparative example 3

A preparation method of a nourishing and bacteriostasis liquid is carried out according to the method in the embodiment 2, and the difference is that: in the preparation step of the nourishing liquid, the extract of the dichondra repens replaces acetyl hexapeptide-8 with equal amount.

Comparative example 4

A preparation method of a nourishing and bacteriostasis liquid is carried out according to the method in the embodiment 2, and the difference is that: in the preparation step of the maintenance liquid, the apple stem cell freeze-dried powder is not added.

Comparative example 5

A preparation method of a nourishing and bacteriostasis liquid is carried out according to the method in the embodiment 2, and the difference is that: in the preparation step of the curing liquid, the acetyl hexapeptide-8 and the pegamun avium extract are equivalently substituted for the apple stem cell freeze-dried powder, and the mass ratio of the acetyl hexapeptide-8 to the pegamun avium extract is 1: 2.5.

comparative example 6

A preparation method of a nourishing and antibacterial liquid is carried out according to the method in the embodiment 2, and is characterized in that indigo naturalis, dogwood and caramel calyx Ma are not added in the step of preparing the extract.

The application provides a method for using the maintenance bacteriostat liquid, which comprises the following steps: cleaning hands and vulva before use, lying one part of the product, absorbing liquid with the aid of an auxiliary device, pushing into the vulva, and standing for a while.

Performance detection

1. Test for bacteriostasis test

The maintenance antibacterial liquid prepared in the examples of the application is used as a test sample, the maintenance antibacterial liquid prepared in the comparative examples of the application is used as a reference sample, and the average antibacterial rates of escherichia coli, staphylococcus aureus and candida albicans are respectively detected by referring to the part GB15979-2002 hygienic Standard for Disposable sanitary articles C4, wherein the antibacterial rate detection results when the antibacterial agent acts for 5min and 20min are recorded as shown in the following tables 1 and 2, the table 1 is the detection result of the examples, and the table 2 is the detection result of the comparative examples.

Table 1 test results of examples

As can be seen from the detection results in Table 1 above, the maintenance bacteriostatic solution prepared in the application has excellent bacteriostatic performance.

Table 2: comparative example test results

Combining the detection results in the above table 1 and table 2, it can be seen that, when the acetyl hexapeptide-8 and the peganum distichum extract are not added in the comparative example 1, the bacteriostatic performance of the prepared maintenance bacteriostatic solution is far lower than the detection result in the example 2; in combination with the detection results of comparative examples 2 and 3, it can be seen that when only acetyl hexapeptide-8 or the extracts of the dierwort are added to the curing solution, the antibacterial performance is poor, and when the acetyl hexapeptide-8 and the extracts of the dierwort are added to the curing solution in the example 2, the antibacterial performance is remarkably improved; referring to the detection results of comparative example 4 and comparative example 5, it can be seen that when the apple stem cell freeze-dried powder is not added into the curing liquid, the bacteriostatic performance is slightly reduced.

2. Vaginal mucosa irritation test

A vaginal mucosa stimulation test is carried out according to the method in 2002 edition of Disinfection technical Specification of Ministry of health, the test animals provide 2.0-2.5kg of female big-ear white rabbits of Japan, the weight of the female, and the dosage of the test substances is 2 g/rabbit by the experimental animal center of medical college of Sigan traffic university, and the test method comprises the following steps: during the test, the animals are fixed in the supine position, 2 g/animal of the tested object is injected into the tested object group through the vagina, the same volume of normal saline is given to the control group, and the animals are fixed in the supine position for 4 hours, so that the tested object is fully contacted with the vaginal mucosa. After the test is finished, the animals are put back into cages for feeding, the animals are sacrificed after 24 hours, vaginal tissues are taken out, the phenomena of mucosal degeneration, secretion, vascular congestion, edema and the like are carefully observed by naked eyes, the conditions of the mucosal degeneration, the secretion, the vascular congestion and the edema of the animals injected with the physiological saline are counted into 0 point, the other animals are compared with the animals injected with the physiological saline and scored, the score is 0-5 points, and the larger the score is, the more serious the damage is. The test results are shown in table 3 below.

TABLE 3 vaginal mucosa irritation test

From the above table 3, it can be seen that none of the bacteriostatic maintenance solutions prepared in the examples of the present application was irritating to the vaginal mucosa, the comparative example 2, in which acetyl hexapeptide-8 was substituted for the soot extract, was slightly irritating, the comparative example 5, in which apple stem cell lyophilized powder was substituted for the soot extract and acetyl hexapeptide-8, was also slightly irritating, and it can be seen that the irritation was reduced due to the excessive addition of acetyl hexapeptide-8 in the comparative examples 2 and 5, or the soot extract, and the examples of the present application, in which acetyl hexapeptide-8 and soot extract were added, was not irritating to the vaginal mucosa.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.