阅读说明：本技术 一种用于检测高原适应性的snp标志物及试剂盒 (SNP marker and kit for detecting plateau adaptability ) 是由 周钢桥 平杰 张红星 李元丰 刘信燚 谢成勇 姜雨晴 于 2021-07-08 设计创作，主要内容包括：本发明涉及一种用于检测高原适应性的SNP标志物及试剂盒,属于生物技术领域。所述SNP标志物为rs1204168,rs1204168为CC或TC基因型指示人体具有高原适应性。采用Sanger测序法时,前向引物序列为核苷酸序列表中的SEQID No.1；后向引物序列为核苷酸序列表中的SEQ ID No.2；采用Sequenom基因分型法时,前向引物序列为核苷酸序列表中的SEQ ID No.3,后向引物序列为核苷酸序列表中的SEQ ID No.4,延伸引物序列为核苷酸序列表中的SEQ ID No.5。所述SNP标志物及试剂盒通过检测rs1204168的基因型可有效检测人体的高原适应性。(The invention relates to an SNP marker and a kit for detecting plateau adaptability, belonging to the technical field of biology. The SNP marker is rs1204168, and the CC or TC genotype of the rs1204168 indicates that the human body has high altitude adaptability. When a Sanger sequencing method is adopted, the sequence of the forward primer is SEQID No.1 in a nucleotide sequence table; the backward primer sequence is SEQ ID No.2 in the nucleotide sequence table; when the Sequenom genotyping method is adopted, the forward primer sequence is SEQ ID No.3 in the nucleotide sequence table, the backward primer sequence is SEQ ID No.4 in the nucleotide sequence table, and the extension primer sequence is SEQ ID No.5 in the nucleotide sequence table. The SNP marker and the kit can effectively detect the plateau adaptability of a human body by detecting the genotype of rs 1204168.)

1. An SNP marker for detecting plateau suitability, characterized by: the SNP marker is rs1204168, and the genotype of the rs1204168 which is CC or TC is an indication that a human body has high adaptability.

2. The SNP marker for detecting plateau adaptability according to claim 1, wherein: the sequence of a forward primer used by the Sanger sequencing method is SEQ ID No.1 in a nucleotide sequence table; the backward primer sequence is SEQ ID No.2 in the nucleotide sequence table.

3. The SNP marker for detecting plateau adaptability according to claim 1, wherein: the forward primer sequence used by the Sequenom genotyping method is SEQ ID No.3 in the nucleotide sequence table, the backward primer sequence is SEQ ID No.4 in the nucleotide sequence table, and the extension primer sequence is SEQ ID No.5 in the nucleotide sequence table.

4. A kit for detecting plateau adaptability, which is characterized in that: the kit comprises the SNP marker rs1204168 for detecting the altitude adaptability according to claim 1, wherein the genotype of the rs1204168 as CC or TC is an indication that the human body has the altitude adaptability.

5. The kit for detecting plateau suitability according to claim 4, wherein: the kit also comprises a forward primer and a backward primer used by the Sanger sequencing method, wherein the sequence of the forward primer is SEQ ID No.1 in a nucleotide sequence table; the sequence of the backward primer is SEQ ID No.2 in the nucleotide sequence table.

6. The kit for detecting plateau suitability according to claim 4, wherein: the kit also comprises a forward primer, a backward primer and an extension primer used by the Sequenom genotyping method, wherein the sequence of the forward primer is SEQ ID No.3 in a nucleotide sequence table, the sequence of the backward primer is SEQ ID No.4 in the nucleotide sequence table, and the sequence of the extension primer is SEQ ID No. 5.

7. The kit for detecting plateau suitability according to claim 4, wherein: the kit also comprises a forward primer and a backward primer used by the Sanger sequencing method, wherein the sequence of the forward primer is SEQ ID No.1 in a nucleotide sequence table; the sequence of the backward primer is SEQ ID No.2 in the nucleotide sequence table;

the kit also comprises a forward primer, a backward primer and an extension primer used by the Sequenom genotyping method, wherein the sequence of the forward primer is SEQ ID No.3 in a nucleotide sequence table, the sequence of the backward primer is SEQ ID No.4 in the nucleotide sequence table, and the sequence of the extension primer is SEQ ID No. 5.

8. The kit for detecting plateau suitability according to any one of claims 4 to 7, wherein: the detection sample of the kit is blood, skin or subcutaneous tissue of a tested human body.

Technical Field

The invention relates to an SNP marker and a kit for detecting plateau adaptability, belonging to the technical field of biology.

Background

In recent years, with rapid development of economy in plateau areas and prevalence of tourism industry, plateau population for immigration has increased day by day. However, the hypoxic environment of a plateau presents challenges to people entering the plateau. The non-plateau population living in the world is very easy to induce acute and chronic altitude diseases when entering into the plateau hypoxia environment. Therefore, the method for predicting the adaptive capacity of the individual plateau environment is expected to provide scientific guidance for tourism, work and the like in plateau areas. Plateau adaptability has obvious genetic basis. The Tibetan population living in the plateau of the world adapts to the severe environments of hypoxia, high cold and the like on the Qinghai-Tibet plateau, lives in generations, transmits dominant genetic genes in generations, and is the population with the highest living altitude and the longest adaptation history among the population living in the plateau of the world. The most common polymorphisms in human genome Deoxyribonucleic Acid (DNA) sequences include Single Nucleotide Polymorphism (SNP), which is the genetic basis for differences in body phenotypes and environmental suitability. Therefore, the prediction of the human plateau suitability based on the SNP information is expected to be realized.

With the gradual development of molecular evolution analysis based on SNP chip technology, High throughput sequencing technology and 'neutral theory inspection', the research of High Altitude Adaptation (HAA) related genomics of human beings is also advancing greatly. Multiple reports indicate that the polymorphism of the EPAS1 gene and the EGLN1 gene is an important genetic basis for Tibetan population to adapt to the hypoxic environment of plateau regions and avoid plateau diseases. However, the relation between the gene MCUR1 of rs1204168 and plateau adaptability and the use thereof as a marker of plateau adaptability are not reported, and the application of rs1204168 is not related in the existing patents.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide an SNP marker for detecting the adaptability to the altitude.

The invention also aims to provide a kit for detecting the plateau adaptability.

In order to achieve the purpose of the invention, the following technical scheme is provided.

An SNP marker for detecting plateau adaptability, the SNP marker is rs1204168, the rs number represents the number of the locus in a dbSNP database, based on the 19 th version of a human genome, the rs1204168 is positioned at the 13,836,672 th position of the 6 th chromosome of a human, and the genotype of the rs1204168, which is CC or TC, is an indication that a human body has plateau adaptability.

Further, the sequence of the forward primer used in the Sanger sequencing method is the nucleotide sequence indicated by the sequence identifier 1 represented by the number identifier <210> in the nucleotide sequence table, i.e. SEQ ID No.1 (SEQ ID No.1 for short) in the nucleotide sequence table; the backward primer sequence is SEQ ID No.2 (SEQ ID No.2 for short) in the nucleotide sequence table; the SNP marker rs1204168 of the invention can be effectively detected by using a Sanger sequencing method by utilizing the forward primer and the backward primer.

Further, the forward primer sequence used by the Sequenom genotyping method is SEQ ID No.3 (SEQ ID No.3 for short) in the nucleotide sequence table, the backward primer sequence is SEQ ID No.4 (SEQ ID No.4 for short) in the nucleotide sequence table, and the extension primer sequence is SEQ ID No.5 (SEQ ID No.5 for short) in the nucleotide sequence table; the SNP marker rs1204168 of the invention can be effectively detected by using a Sequenom genotyping method by using the forward primer, the backward primer and the extension primer.

A kit for detecting plateau adaptability comprises an SNP marker rs1204168 for detecting plateau adaptability, wherein the genotype of rs1204168, which is CC or TC, is an indication that a human body has plateau adaptability.

Further, the kit also comprises a forward primer with a sequence of SEQ ID number 1 and a backward primer with a sequence of SEQ ID NO.2, which are used in the Sanger sequencing method; the SNP marker rs1204168 can be effectively detected by using a Sanger sequencing method by utilizing the forward primer and the backward primer.

Further, the kit also comprises a forward primer with a sequence of SEQ ID NO.3, a backward primer with a sequence of SEQ ID NO.4 and an extension primer with a sequence of SEQ ID NO.5, which are used by the Sequenom genotyping method; the SNP marker rs1204168 of the invention can be effectively detected by using a Sequenom genotyping method by using the forward primer, the backward primer and the extension primer.

Furthermore, the detection sample of the kit is blood, skin or subcutaneous tissue of a tested human body.

Advantageous effects

1. The invention provides an SNP marker for detecting plateau adaptability, the SNP marker is rs1204168, and the genotype of rs1204168 as CC or TC can be used as the indication that a human body has plateau adaptability.

2. The invention provides an SNP marker for detecting plateau adaptability, and the SNP marker rs1204168 can be effectively detected by using a Sanger sequencing method by utilizing a forward primer and a backward primer.

3. The invention provides an SNP marker for detecting plateau adaptability, and the SNP marker rs1204168 can be effectively detected by a Sequenom genotyping method by utilizing a forward primer, a backward primer and an extension primer.

4. The invention provides a kit for detecting plateau adaptability, which comprises an SNP marker rs1204168 and primers used for detecting the SNP marker rs1204168 by using a Sanger sequencing method or a Sequenom genotyping method, and can be used for detecting the genotype of the rs1204168 by the kit so as to effectively detect whether a human body has plateau adaptability.

Drawings

FIG. 1 shows F of Tibetan population and Han population in example 1_STThe results are manhattan plots.

FIG. 2 is a plot of the frequency of rs1204168 genotype measured in example 3 for the subjects of example 2.

FIG. 3 is a graph of rs1204168 genotype vs. hemoglobin concentration for example 4.

Detailed Description

The invention will be described in detail below with reference to the drawings and embodiments, examples of which are illustrated in the drawings. The embodiments described by referring to the drawings are exemplary and intended to be illustrative of the invention and are not to be construed as limiting the invention.

Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and may be performed with reference to molecular cloning, laboratory Manual, third edition or related products. The kit products used are also commercially available. Various procedures and methods not described in detail are conventional methods well known in the art, and the sources, trade names, and components of the reagents used are indicated at the time of first appearance, and the same reagents used thereafter are the same as those indicated at the first appearance, unless otherwise specified. Example 1 Whole genome evolution analysis study

Study subjects: genomic DNA of 48 Tibetan and 50 Han. Genomic DNA of 48 Tibetan persons, which are respectively derived from peripheral blood of 48 Tibetan persons without relativity, live in 5 cities with an altitude of 2,600 m to 4,200 m in the autonomous State of Cuminum celery of Sichuan province, have a Qinghai Chronic altitude disease Score (Qinghai scientific Mountain Sickness Score) of less than 5 minutes, and are healthy and free of altitude disease. The genomic DNA of 50 Han people, respectively, is derived from the peripheral blood of 50 Han people without relativity, the Han people live in south-ning City, Guangxi province, with an average altitude of 79 m, and the specific information is shown in Table 1.

And (3) detecting the genotype information of the rs1204168 locus of the research object by whole genome sequencing.

The subjects were subjected to an evolutionary analysis study using a fixed factor (Fixation Index, F)_ST) Test methods and additive model-based Logistic regression analysis methods. Wherein, F_STThe VCFTools software was used to calculate for checking the genetic distance between tibetan and han. Logistic regression was calculated using the PLINK software version 1.07 with rs1204168 genotype as the independent variable, TT ═ 0, TC ═ 1, CC ═ 2, phenotype (ethnic group) as the dependent variable, corrected for gender, age, and used to examine the frequency difference of rs1204168 between tibetan and han.

TABLE 1 population information summary table for genome-wide SNP frequency difference study

Evolution analysis research results:

genome-wide mean F_STThe genetic distance between two populations can be assessed, and this example found the genetic distance between the Tibetan and Han families, i.e., the whole genome mean F_STIs 0.011. SNP F_STThe larger the value, the more likely these SNPs are to be positively selected (Positive Selection). In this example, sample F_STThe top 1,500 SNP serves as the SNP threshold for potential positive selection. F of these SNPs_STValue (F)_STNot less than 0.235) is higher than the whole genome F_ST5 standard deviations (F) above the mean value_ST>0.146). Wherein, the first SNP, F thereof, is excluded_STValue (F)_ST0.565) higher than the mean by 20 standard deviations (F)_ST>0.551), the first ranked in the new signal is the 6p23 region (index SNP rs1204168, F)_ST0.464) the natural selection signal region contains 14F_STVery significant SNPs (F)_ST>0.42), and the region and nearby genes have not been reported to be associated with plateau adaptability. From F_STThe results of the test showed that the genomic genetic distance was small for Tibetan and Han across most SNPs, as shown in FIG. 1; however, in rs1204168, F in rs1204168 was observed_STA value of 0.464 suggests that rs1204168 is a SNP with a strong positive selection probability, i.e., the C allele of rs1204168 is highly likely to be associated with positive selection by tibetan people to adapt to the plateau environment.

The results of logistic regression showed that rs1204168 is an SNP with a large frequency difference between Tibetan and Han, and the statistical test thereof shows that OR is 0.08759 and P is 1.11 × 10^-7. The higher frequency variation carried by the Tibetan is the C allele of rs 1204168.

The combination of the above results proves that the C allele of rs1204168 is closely related to the plateau adaptability of the individual.

Example 2 genotyping of rs1204168 in subjects Using Sanger sequencing method

Study subjects: 48 Tibetan human genomic DNA samples from example 1. Sanger sequencing the PCR amplification product of the DNA sample using 3130xl Genetic Analyzer (ABI, Foster City, Calif.) to obtain a sequencing result; determining rs1204168 genotype of the DNA sample based on the sequencing result. In the Sanger sequencing method, the forward and backward primers used for genotyping rs1204168 were as follows:

the sequence of the forward primer is as follows: TATGAGACCACCGAGATTTG (SEQ ID NO.1),

the sequence of the backward primer is as follows: GCCTTCACATTGTTTCCC (SEQ ID NO. 2).

The sequencing result shows that: the DNA samples respectively present CC genotypes and TT genotypes, which indicates that Sanger sequencing can effectively distinguish different genotypes of rs 1204168.

Example 3 frequency of rs1204168 in independent population

Study subjects: 672 samples of genomic DNA from Tibetan and 270 samples of Han. The Tibetan nationality is all living in Tibetan Lasa city with an average altitude of 3,650 m. The Chinese people come from a plurality of provinces and cities of the whole country, and the altitude is below 2,500 meters. All individuals have no genetic relationship, and the specific information is shown in Table 2. All individuals had signed informed consent, and the study was approved for performance by the ethical committee of the institute of radiology.

Primers are designed aiming at rs1204168, and rs1204168 mutation conditions are obtained through Sequenom detection. The specific steps of Sequenom detection are as follows:

designing PCR primers and extension primers aiming at rs1204168 to be typed in the genome DNA sample of the researched object. This process was implemented using MassARRAY Design 3.0 software. Approximately 15ng of genomic DNA per sample was used per experiment. DNA template amplification was performed using PCR experiments, and the amplified product and extension primers were used for specific single base extension reactions at SNP sites. The reaction products were transferred to a SpectroCHIP chip (Sequenom, Calif., USA) via an automated spotter. Genotyping was performed on the Sequenom nucleic acid mass spectrometry platform (Sequenom, ca, usa), off-line data, and analyzed using MassARRAY TYPER software (Sequenom, ca, usa). In the Sequenom assay, primers used to genotype rs1204168 gene were as follows:

a forward primer: ACGTTGGATGTGTGAATGGTGGCCCTTCAG (SEQ ID NO: 3);

and (3) backward primer: ACGTTGGATGAAGTGTGTTTCTGGCTTCTC (SEQ ID NO: 4);

extending a primer: GGCTTCTCTCTTGGAAAG (SEQ ID NO: 5).

Table 2 crowd information table for example 3

As a result: the C allele frequency of rs1204168, as determined by counting, was 75% in the Tibetan population and 47% in the Han population. That is, the frequency of the C allele of rs1204168 is different between the Tibetan population and the Han population, as shown in FIG. 2.

Example 4 correlation of genotype of rs1204168 with hemoglobin concentration

The hemoglobin concentration refers to the amount of hemoglobin contained in a unit volume (L) of blood. Hemoglobin is a pigment-containing binding protein, which is the main component of erythrocytes and can bind oxygen, transporting oxygen and carbon dioxide. The increased hemoglobin concentration is divided into physiological and pathological. The former is common to people living in plateau areas, and because of the relatively low oxygen concentration in plateau areas, the body of people living there for a long time generates more hemoglobin in a compensatory manner. However, the increase and decrease of hemoglobin concentration is consistent with the increase and decrease of erythrocyte concentration, and when the hemoglobin concentration exceeds the threshold value after the organism is decompensated, the organism suffers from erythrocytosis, that is, excessive increase of hemoglobin concentration is closely related to the occurrence of altitude disease.

Study subjects: genomic DNA samples of 270 individuals in example 3 were used. Designing a primer aiming at rs1204168, and obtaining a base of rs1204168 through Sequenom detectionIt is of type. Hemoglobin concentration using Hemocue Hb 201⁺The analyzer (en el hall, sweden). The correlation between the genotype of rs1204168 and hemoglobin concentration was evaluated by pearson correlation analysis, and in the rs1204168 genotype, TT ═ 0, TC ═ 1, and CC ═ 2 were set. When P is present<0.05 was considered to have a significant statistical significance.

As a result: to explore whether rs1204168 could serve as a biomarker, correlation analysis was performed using the C allele frequency of rs1204168 and hemoglobin concentration. The inventors demonstrated that the C allele frequency of rs1204168 is significantly inversely correlated with hemoglobin concentration: r is-0.141 and P is 0.0199, as shown in fig. 3, where n represents the number of people. The higher the C allele frequency is, the lower the hemoglobin concentration is, and further, if the genotype of the rs1204168 site of the individual is CC or TC, the individual is considered to have certain high adaptability.

Nucleotide sequence listing

<110> military medical research institute of military science institute of people's liberation force of China

<120> SNP marker and kit for detecting plateau adaptability

<160> 5

<210> 1

<211> 20

<212> DNA

<213> Artificial sequence

<220>

<223> Forward primer for Sanger sequencing

<400>

tatgagacca ccgagatttg 20

<210> 2

<211> 18

<212> DNA

<213> Artificial sequence

<220>

<223> reverse primer for Sanger sequencing

<400>

gccttcacat tgtttccc 18

<210> 3

<211> 30

<212> DNA

<213> Artificial sequence

<220>

<223> Forward primer for Sequenom sequencing

<400>

acgttggatg tgtgaatggt ggcccttcag 30

<210> 4

<211> 30

<212> DNA

<213> Artificial sequence

<220>

<223> reverse primer for Sequenom sequencing

<400>

acgttggatg aagtgtgttt ctggcttctc 30

<210> 5

<211> 30

<212> DNA

<213> Artificial sequence

<220>

<223> extended primer for Sequenom sequencing

<400>

ggcttctctc ttggaaag 18