阅读说明：本技术 核酸扩增和识别方法 (Nucleic acid amplification and identification method ) 是由 Y·戈佩尔 P·莫尔 T·睿达 A·塞茨 于 2019-12-13 设计创作，主要内容包括：本发明提供了一种生成核酸模板的标记的扩增片段的方法,包括提供所述模板核酸；使至少一种寡核苷酸引物与所述模板核酸退火；以模板特异性方式延伸至少一种寡核苷酸引物从而产生延伸产物,其中当延伸产物到达模板核酸的5’端或达到与延伸产物下游的模板核酸退火的延伸终止子时,所述延伸反应停止；提供衔接子核酸,其5’端包含识别序列,所述识别序列在与延伸终止子接触时不与延伸终止子杂交；将衔接子核酸的5’端连接到延伸产物的3’端,从而产生标记的扩增片段。(The present invention provides a method of generating a labelled amplified fragment of a nucleic acid template comprising providing said template nucleic acid; annealing at least one oligonucleotide primer to the template nucleic acid; extending at least one oligonucleotide primer in a template-specific manner to produce an extension product, wherein the extension reaction stops when the extension product reaches the 5' end of the template nucleic acid or reaches an extension terminator that anneals to the template nucleic acid downstream of the extension product; providing an adaptor nucleic acid comprising at its 5' end a recognition sequence that does not hybridize to the extension terminator when contacted with the extension terminator; the 5 'end of the adaptor nucleic acid is ligated to the 3' end of the extension product, thereby generating labeled amplified fragments.)