阅读说明：本技术 微生物挥发性物质对植物作用的盆栽检测装置及检测方法 (Potted plant detection device and detection method for action of microbial volatile substances on plants ) 是由 姜明国 王锟 丰景 王金子 高亚慧 于 2021-09-29 设计创作，主要内容包括：本发明公开了一种微生物挥发性物质对植物作用的盆栽检测装置及检测方法,通过将植物栽培区和微生物培养区组合一体式设计,解决了既要保证微生物纯培养又要便于观察其挥发性代谢产物对植物作用效果的问题。植物栽培区多孔结构使挥发性代谢产物自由通过接触根系,蓄水区保持土壤湿度。微生物培养区由环形隔板阻隔形成环形区域环绕植物培养区,保证挥发性代谢产物直接作用于植物根系。连接结构使植物栽培和微生物培养一体式容器与底罐紧密连接,灭菌后使底罐内腔处于无菌环境,防止杂菌污染。本发明适用于在微生物挥发性代谢产物对植物作用方面的盆栽实验检测的研究。(The invention discloses a potted plant detection device and a detection method for the action of a microorganism volatile substance on a plant. The porous structure of the plant cultivation area enables volatile metabolites to freely pass through and contact the root system, and the water storage area keeps soil humidity. The microorganism culture area is blocked by an annular clapboard to form an annular area surrounding the plant culture area, so that the volatile metabolite is ensured to directly act on the plant root system. The connecting structure enables the plant cultivation and microorganism cultivation integrated container to be tightly connected with the bottom tank, and the inner cavity of the bottom tank is in a sterile environment after sterilization, so that the pollution of mixed bacteria is prevented. The invention is suitable for the research of the detection of the pot experiment in the aspect of the action of the volatile metabolite of the microorganism on the plant.)

1. The utility model provides a detection device cultivated in a pot of microorganism volatile substance to plant effect which characterized in that: including integral type container, connection structure, end jar includes end jar inner chamber, end jar mouth, connection structure connects the top that sets up at the integral type container, end jar mouth passes through connection structure and integral type container swing joint.

2. The apparatus for detecting potting of a plant affected by a volatile substance of a microorganism of claim 1, wherein: the integrated container includes: the device comprises a plant cultivation area, a microorganism cultivation area, a porous structure and a water storage area, wherein the plant cultivation area is positioned in the center of an integrated container, and an opening of the plant cultivation area is upward; the microorganism culture area is positioned at the periphery of the plant culture area, and the opening of the microorganism culture area is downward; the porous structure is positioned at the lower part of the plant cultivation area, and the water storage area is positioned at the bottom of the plant cultivation area.

3. A device for detecting potting of a plant with a microbial volatile material as claimed in claim 2, wherein: the microorganism culture area comprises an outer partition plate and an inner partition plate, wherein the outer partition plate and the inner partition plate are both of annular structures, a space formed by the outer partition plate and the inner partition plate is the culture area, and an opening of the culture area faces downwards.

4. The apparatus for detecting potting of a plant affected by a volatile substance of a microorganism of claim 1, wherein: the bottom tank opening is hermetically connected with the connecting structure.

5. The apparatus for detecting potting of a plant affected by a volatile substance of a microorganism of claim 1, wherein: the material of the integrated container is one of glass, ceramic and plastic.

6. The method for detecting the potted plant effect of the volatile substances in the microorganisms according to claim 1, wherein the application process of the detection method comprises the following steps:

1) a pretreatment process: preparation and pretreatment of the potted plant detection device and required experimental materials, including configuration and sterilization operations: preparing a solid culture medium required by microbial culture, culture soil and water required by plant growth; sterilizing materials with the required sterility requirements for experiments, including sterilizing a pot detection device, sterile water, culture soil and a solid culture medium;

2) the disassembly and assembly process of the potted plant detection device is as follows: the integrated container and the bottom tank of the potted plant detection device are disassembled and connected and combined through a connecting structure;

3) the filling process of the contents: filling a solid culture medium required by a microorganism culture area and culture soil required by a plant culture area, and filling the pretreated solid culture medium and the culture soil into an integrated container;

4) inoculation process of experimental materials: inoculating the target strain on a solid culture medium in a microorganism culture area, and planting plant seeds or seedlings in different growth stages in culture soil in a plant culture area;

5) and (3) post-treatment process: the experimental maintenance required operations comprise temperature and humidity control, plant irrigation, illumination control and sterile external environment control;

6) and (3) detection process: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms are contacted with a plant root system through the porous structure, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

7. The method for detecting the plant-action potted plant detection device by the microbial volatile substances as claimed in claim 6, wherein the detection method comprises the following specific steps:

step 1: the bottom tank is hermetically connected with the connecting structure through a bottom tank opening and is connected and combined with the integrated container; a cavity is formed in the inner cavity of the bottom tank, the opening of the plant cultivation area is upward, a sealing cover or a covering film is added to seal the opening of the plant cultivation area, a sealed inner environment is formed in the inner cavity of the bottom tank, and a sterile inner environment is formed after sterilization;

step 2: the integrated container is placed on an ultra-clean workbench after ultraviolet sterilization, the bottom tank is opened, then the solid culture medium after high-temperature sterilization is poured into a microorganism culture area, and is hermetically connected with the connecting structure through the opening of the bottom tank, and the bottom tank and the integrated container are connected and combined to keep an aseptic internal environment;

step 3: after the solid culture medium is cooled, opening the bottom tank, inoculating the target strain on the solid culture medium, hermetically connecting the bottom tank with the connecting structure through a bottom tank opening, connecting and combining the bottom tank with the integrated container, and placing the integrated container in the forward direction;

step 4: after the bacterial colony culture is finished, opening a sealing cover or covering a film, filling sterilized culture soil, planting seeds or seedlings in different growth stages, and adding sterile water, so that the water in a water storage area at the bottom cannot overflow from a porous structure while the soil is infiltrated;

step 5: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms are contacted with a plant root system through the porous structure, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

8. The method for detecting the plant-action potted plant detection device by the microbial volatile substances as claimed in claim 6, wherein the detection method comprises the following specific steps:

step 1: after the plant cultivation area is filled with culture soil, a sealing cover or a film is added for sealing, the bottom tank opening is hermetically connected with the connecting structure, the bottom tank is connected and combined with the integrated container, and a sealed inner environment is formed in the inner cavity of the bottom tank;

step 2: putting the integrated container into a sterilization pot for integral sterilization, and forming an aseptic environment in the inner cavity of the bottom tank after sterilization;

step 3: the integrated container is placed on a superclean bench upside down, the high-temperature sterilized solid culture medium is poured into the microorganism culture area, the bottom tank is opened after the solid culture medium is cooled, the target strain is inoculated on the culture medium, the bottom tank is hermetically connected with the connecting structure through the opening of the bottom tank, the bottom tank and the integrated container are connected and combined, and the integrated container is placed in the forward direction;

step 4: after the bacterial colony culture is finished, opening a sealing cover or a covering film, planting seeds or seedlings in different growth stages and adding sterile water;

step 5: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms are contacted with a plant root system through the porous structure, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

9. The method for detecting a plant pot culture detection device by using a volatile substance of a microorganism according to claim 6, wherein the method comprises the steps of: the sterilization method of the sterile water is one of a steam method, a UHT thermal method, an ultraviolet sterilization method, an ozone method and a physical filtration method.

Technical Field

The invention relates to the technical field of research on plant growth effects of microorganisms, in particular to a potted plant detection device and a detection method for effects of volatile substances of microorganisms on plants.

Background

The beneficial microorganisms promote the growth and development of plants through direct action modes such as generating hormone substances, promoting the absorption of plant nutrients, regulating plant metabolism, inducing and activating resistance genes, modifying and regulating key gene expression and the like, or indirect action modes such as directly inhibiting the growth of pathogenic bacteria and the like. In the research of tobacco, 1, 3-butanediol which is a volatile metabolite generated by symbiotic enterobacter cloacae can obviously promote the growth of arabidopsis plants and can regulate root configuration, lateral root number and main root length. Researches show that the microbial volatile secondary metabolites can regulate plant metabolism in multiple ways, the volatile substances generated by the bacillus subtilis can inhibit feedback regulation of sugar accumulation by inducing the reduction of the production amount of abscisic acid in arabidopsis thaliana, increase the chlorophyll content of arabidopsis thaliana, improve the photosynthetic efficiency and increase nutrient accumulation, and can increase the production amount of arabidopsis thaliana root auxin and up-regulate the expression amount of auxin transport genes. The volatile substances produced by bacillus subtilis, agrobacterium tumefaciens and pseudomonas syringae can promote the accumulation of starch in plants by increasing the activity of sucrose synthase, starch synthase and starch branching enzyme in plants. The plant volatile metabolite can also induce the plant to activate the resistance gene to generate plant induced resistance, the volatile metabolite of the bacillus subtilis acts on arabidopsis thaliana, the expression quantity of the related gene of the jasmonic acid metabolism pathway and the related gene of the salicylic acid metabolism pathway is obviously improved, and the two metabolism pathways can cause the plant induced resistance. Researches show that the microbial volatile metabolites can be used as signal molecules for regulating the interaction between plants and microbes, can promote the growth of the plants, induce the plants to resist biotic stress or abiotic stress, and can also directly kill or inhibit pathogenic microorganisms to induce the plants to generate disease resistance.

At present, a two-cell culture dish culture method is usually adopted for carrying out a flat plate experiment for researching the effect of the volatile metabolites of the microorganisms on the growth of plants, and the culture method has the defects that the two-cell culture dish has limited space, can only research the effect of the volatile metabolites of the microorganisms at the initial growth stage of the plants, and cannot be applied to the plants with fast growth and large body size. Meanwhile, the stem and root system is in an environment, the action sites of volatile metabolites are difficult to distinguish, and the volatile metabolites are difficult to act on the root system through the culture medium.

The problems that the microorganism and the plant are difficult to co-culture, the volatile metabolites of the microorganism are difficult to directly act on the root system of the plant, the interaction phenomenon of the microorganism and the plant is difficult to directly observe and the like exist in the co-culture process of the plant and the microorganism. At present, no fixed experimental device exists in the field of research on the action of microbial volatile metabolites on plants, and a potted plant experiment detection device is required for practical application.

The design of a potting device with microbial volatile substances acting on plants becomes a problem to be solved urgently.

Disclosure of Invention

In order to solve the technical problems, the invention provides a potted plant detection device for the action of microorganism volatile substances on plants and a detection method thereof.

According to one aspect of the invention, the potted plant detection device comprises an integrated container, a connecting structure and a bottom tank, wherein the bottom tank comprises a bottom tank inner cavity and a bottom tank opening, the connecting structure is connected to the top of the integrated container, and the bottom tank opening is movably connected with the integrated container through the connecting structure.

Further, the integrated container includes: the device comprises a plant cultivation area, a microorganism cultivation area, a porous structure and a water storage area, wherein the plant cultivation area is positioned in the center of an integrated container, and an opening of the plant cultivation area is upward; the microorganism culture area is positioned at the periphery of the plant culture area, and the opening of the microorganism culture area is downward; the porous structure is positioned at the lower part of the plant cultivation area, and the water storage area is positioned at the bottom of the plant cultivation area.

Further, the microorganism culture area comprises an outer partition plate and an inner partition plate, the outer partition plate and the inner partition plate are both of annular structures, a space formed by the outer partition plate and the inner partition plate is the culture area, and an opening of the culture area faces downwards.

Further, the bottom tank opening is hermetically connected with the connecting structure.

Further, the material of the integrated container is one of glass, ceramic and plastic.

The detection method of the detection device for the plant pot culture by the volatile substances of the microorganisms comprises the following application processes:

1) a pretreatment process: preparation and pretreatment of the potted plant detection device and required experimental materials, including configuration and sterilization operations: preparing a solid culture medium required by microbial culture, culture soil and water required by plant growth; sterilizing materials with the required sterility requirements for experiments, including sterilizing a pot detection device, sterile water, culture soil and a solid culture medium;

2) the disassembly and assembly process of the potted plant detection device is as follows: the integrated container and the bottom tank of the potted plant detection device are disassembled and connected and combined through a connecting structure;

3) the filling process of the contents: filling a solid culture medium required by a microorganism culture area and culture soil required by a plant culture area, and filling the pretreated solid culture medium and the culture soil into an integrated container;

4) inoculation process of experimental materials: inoculating the target strain on a solid culture medium in a microorganism culture area, and planting plant seeds or seedlings in different growth stages in culture soil in a plant culture area;

5) and (3) post-treatment process: the experimental maintenance required operations comprise temperature and humidity control, plant irrigation, illumination control and sterile external environment control;

6) and (3) detection process: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms are contacted with a plant root system through the porous structure, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

Further, the detection method comprises the following specific steps:

step 1: the bottom tank is hermetically connected with the connecting structure through a bottom tank opening and is connected and combined with the integrated container; a cavity is formed in the inner cavity of the bottom tank, the opening of the plant cultivation area is upward, a sealing cover or a covering film is added to seal the opening of the plant cultivation area, a sealed inner environment is formed in the inner cavity of the bottom tank, and a sterile inner environment is formed after sterilization;

step 2: the integrated container is placed on an ultra-clean workbench after ultraviolet sterilization, the bottom tank is opened, then the solid culture medium after high-temperature sterilization is poured into a microorganism culture area, and is hermetically connected with the connecting structure through the opening of the bottom tank, and the bottom tank and the integrated container are connected and combined to keep an aseptic internal environment;

step 3: after the solid culture medium is cooled, opening the bottom tank, inoculating the target strain on the solid culture medium, hermetically connecting the bottom tank with the connecting structure through a bottom tank opening, connecting and combining the bottom tank with the integrated container, and placing the integrated container in the forward direction;

step 4: after the bacterial colony culture is finished, opening a sealing cover or covering a film, filling sterilized culture soil, planting seeds or seedlings in different growth stages, and adding sterile water, so that the water in a water storage area at the bottom cannot overflow from a porous structure while the soil is infiltrated;

step 5: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms are contacted with a plant root system through the porous structure, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

Further, the detection method comprises the following specific steps:

step 1: after the plant cultivation area is filled with culture soil, a sealing cover or a film is added for sealing, the bottom tank opening is hermetically connected with the connecting structure, the bottom tank is connected and combined with the integrated container, and a sealed inner environment is formed in the inner cavity of the bottom tank;

step 2: putting the integrated container into a sterilization pot for integral sterilization, and forming an aseptic environment in the inner cavity of the bottom tank after sterilization;

step 3: the integrated container is placed on a superclean bench upside down, the high-temperature sterilized solid culture medium is poured into the microorganism culture area, the bottom tank is opened after the solid culture medium is cooled, the target strain is inoculated on the culture medium, the bottom tank is hermetically connected with the connecting structure through the opening of the bottom tank, the bottom tank and the integrated container are connected and combined, and the integrated container is placed in the forward direction;

step 4: after the bacterial colony culture is finished, opening a sealing cover or a covering film, planting seeds or seedlings in different growth stages and adding sterile water;

step 5: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms are contacted with a plant root system through the porous structure, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

Further, the sterilization method of the sterilized water is one of a steam method, a UHT heat method, an ultraviolet sterilization method, an ozone method, and a physical filtration method.

The working principle of the invention is as follows:

the plant cultivation area comprises a porous structure and two auxiliary function areas of a water storage area. The plant cultivation area realizes the functions of freely passing through the volatile metabolites of the microorganisms, keeping the soil humidity, maintaining the plant growth environment and the like. The wall of the plant cultivation area is provided with a porous structure for passing through microorganism volatile metabolites, and the bottom of the plant cultivation area is provided with a water storage area for storing a proper amount of water to keep the soil humidity.

The opening of the plant cultivation area is upward, and the whole bottom is suspended in the inner cavity of the bottom tank. The microorganism culture area is surrounded around the porous structure of the plant culture area wall, so that the distance between the volatile metabolic substances and the root system is the shortest, the volatile metabolic substances are contacted with the root system of the plant through the porous structure once being released, the sensitivity of the experiment is improved, the method not only can be used for qualitative experiment for identifying the effect of the volatile metabolic substances of the microorganism on the growth of the plant, but also can provide conditions for quantitative experiment for measuring the effect of the volatile metabolic substances of the microorganism with different bacterial quantities on the growth of the plant.

The microorganism culture area is formed by blocking an annular outer partition plate and an inner partition plate, a solid culture medium is filled, and an opening faces downwards to prevent mixed bacteria from falling. The microorganism culture area surrounds the plant culture area, so that the volatile metabolic substances of the microorganisms directly contact with the plant root system from the porous structure.

A certain gap is formed between the plant cultivation area and the microorganism cultivation area, and the plant cultivation area is separated from the microorganism cultivation area through the gap formed by the wall of the plant cultivation area and the inner partition plate, so that the sterile environment required by microorganism cultivation is further prevented from being polluted in the plant cultivation process. Connection structure and end jar mouth pass through connected modes such as threaded connection and connect, make integral type container and end jar be airtight connection, form aseptic district in end jar inner chamber after the sterilization, provide the growth condition for microbial cultivation.

The substantive characteristics and the progress of the invention are as follows:

(1) the integral type container, plant cultivation and microbial cultivation integral type device are convenient for operate. The pot detection device, the culture medium and the culture soil are sterilized, and the plants are cultivated by using sterile water, so that the problem that the plant cultivation is influenced by microbial pollution is effectively solved.

(2) The addition of components can be manually controlled in the sterile water, the external environment for plant growth is controlled, the controllability of the experimental process is realized, and the growth process of microorganisms is ensured not to be polluted by mixed bacteria.

(3) In the aspect of detecting the functionality of the microorganism volatile substances on the action of the plants, the types of the microorganisms, the number of the microorganisms, the growth period of the microorganisms, the types of the plants and the growth stage of the plants can be controlled, so that the method can be applied to experiments with different experimental purposes.

(4) The porous structure penetrates through the volatile metabolic substances of the microorganisms, and the water storage function of the water storage area is realized. When watering, water can be accumulated in the water storage area and can not flow into the inner cavity of the bottom tank. The design of the porous structure and the water storage area ensures that the humidity can be kept in plant cultivation, and the volatile metabolic substances of microorganisms can contact plant roots.

(5) The integrated container and the bottom tank are connected in a sealing mode and can be detached freely, bacterial strains can be inoculated conveniently, the internal environment can be controlled conveniently, and sterile environment is formed through sterilization after sealing to maintain microbial culture.

(6) The microorganism culture area is separated from the plant culture area, so that the partition split charging is realized, the pure culture of the microorganism to be detected is not influenced, and the volatile metabolic substances of the microorganism can be contacted with the root system to the maximum extent through the porous structure.

Drawings

FIG. 1 is a schematic structural diagram of a potting detection device for the action of microbial volatile substances on plants according to the present invention.

Fig. 2 is a perspective view of the integrated container.

Fig. 3 is a schematic view of the structure of the bottom tank.

Fig. 4 is a cross-sectional view of fig. 2.

Part numbers and names in the figures:

1. the device comprises an outer partition board, 2 a microorganism culture area, 3 an inner partition board, 4 a plant culture area wall, 5 a water storage area, 6 a connecting structure, 7 a plant culture area, 8 a porous structure, 9 a bottom tank opening, 10 a bottom tank wall and 11 a bottom tank inner cavity.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are for purposes of illustration only and are not to be construed as limitations of the invention. It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings.

In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

In at least one embodiment of the invention, as shown in fig. 1-4, the potted plant detection device for the action of the volatile substances of the microorganisms on the plants comprises an integrated container, a connecting structure 6 and a bottom tank, wherein the bottom tank comprises a bottom tank inner cavity 11 and a bottom tank opening 9, the connecting structure 6 is connected and arranged at the top of the integrated container, and the bottom tank opening 9 is movably connected with the integrated container through the connecting structure 6.

The integrated container includes: the device comprises a plant cultivation area 7, a microorganism cultivation area 2, a porous structure 8 and a water storage area 5, wherein the plant cultivation area 7 is positioned in the center of the integrated container, and the opening of the plant cultivation area 7 is upward; the microorganism culture area 2 is positioned at the periphery of the plant cultivation area 7, and the opening of the microorganism culture area 2 is downward; the porous structure 8 is positioned at the lower part of the plant cultivation area 7, and the water storage area 5 is positioned at the bottom of the plant cultivation area 7.

The position of the porous structure 8 is consistent with the height of the partition board of the microorganism culture area 2, and the hole spacing and the hole row number are adjusted according to the practical application environment.

The microorganism culture area 2 comprises an outer partition plate 1 and an inner partition plate 3, the outer partition plate 1 and the inner partition plate 3 are both of annular structures, the space formed by the outer partition plate 1 and the inner partition plate 3 is a culture area, and an opening of the culture area faces downwards.

The heights of the outer partition board 1 and the inner partition board 3 of the microorganism culture area 2 are equal, the space between the outer partition board 1 and the inner partition board 3 determines the area of the microorganism culture area 2, and the inoculation quantity is determined according to the size of the microorganism culture area 2 and the quantity of volatile metabolites required by the plant growth to be researched.

The bottom tank opening 9 is hermetically connected with the connecting structure 6.

The material of the integrated container is one of glass, ceramic and plastic.

A certain gap is formed between the plant cultivation area 7 and the microorganism cultivation area 2, and the plant cultivation area 7 is separated from the microorganism cultivation area 2 through the gap formed by the plant cultivation area wall 4 and the inner partition plate 3, so that the sterile environment required by microorganism cultivation is further prevented from being polluted in the plant cultivation process. Connection structure 6 and end jar mouth 9 are connected through connected modes such as threaded connection, make integral type container and end jar be airtight connection, form aseptic district in end jar inner chamber 11 after the sterilization, provide the growth condition for microbial cultivation.

The invention has simple design, strong practicability and wide material selectivity range, can be made into a disposable culture device through various sterilization treatments, and is applied to large-scale production in factories. The structure of the bottom tank opening 9 corresponds to the structure of the integrated container connecting structure 6, so that the purpose of sealing the inner cavity 11 of the bottom tank is achieved. The bottom tank is sized according to the size of the integrated container. The integrated container and the bottom tank are made of glass, plastics, ceramics and the like.

The detection method of the detection device for the plant pot culture by the volatile substances of the microorganisms comprises the following application processes:

1) a pretreatment process: preparation and pretreatment of the potted plant detection device and required experimental materials, including configuration and sterilization operations: preparing a solid culture medium required by microbial culture, culture soil and water required by plant growth; sterilizing materials with the required sterility requirements for experiments, including sterilizing a pot detection device, sterile water, culture soil and a solid culture medium;

2) the disassembly and assembly process of the potted plant detection device is as follows: the integrated container and the bottom tank of the potted plant detection device are disassembled and connected and combined through the connecting structure 6;

3) the filling process of the contents: filling a solid culture medium required by the microorganism culture area 2 and culture soil required by the plant culture area 7, and filling the pretreated solid culture medium and the culture soil into an integrated container;

4) inoculation process of experimental materials: the target strain is inoculated on a solid culture medium in the microorganism culture area 2, and plant seeds or seedlings in different growth stages are planted in culture soil in a plant culture area 7;

5) and (3) post-treatment process: the experimental maintenance required operations comprise temperature and humidity control, plant irrigation, illumination control and sterile external environment control;

6) and (3) detection process: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms penetrate through the porous structure 8 to contact with the root system of the plant, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

The first embodiment is as follows:

the integrated container and the bottom tank are made of glass or ceramic materials, and the detection method comprises the following steps:

step 1: the bottom tank is hermetically connected with the connecting structure 6 through a bottom tank opening 9, and the bottom tank is connected and combined with the integrated container; a cavity is formed in the inner cavity 11 of the bottom tank, the opening of the plant cultivation area 7 is upward, after the opening of the plant cultivation area 7 is sealed by a sealing cover or a covering film, a sealed inner environment is formed in the inner cavity 11 of the bottom tank, and a sterile inner environment is formed after sterilization;

step 2: the integrated container is placed on an ultra-clean workbench after ultraviolet sterilization, the bottom tank is opened, then the solid culture medium after high-temperature sterilization is poured into the microorganism culture area 2, and is hermetically connected with the connecting structure 6 through the bottom tank opening 9, the bottom tank and the integrated container are connected and combined, and the sterile internal environment is kept;

step 3: after the solid culture medium is cooled, opening the bottom tank, inoculating the target strain on the solid culture medium, hermetically connecting the bottom tank with the connecting structure 6 through a bottom tank opening 9, connecting and combining the bottom tank with the integrated container, and placing the bottom tank and the integrated container in the forward direction;

step 4: after the bacterial colony culture is finished, opening a sealing cover or covering a film, filling sterilized culture soil, planting seeds or seedlings in different growth stages, and adding sterile water, so that the water quantity of the bottom water storage area 5 is not overflowed from the porous structure 8 while the soil is infiltrated;

step 5: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms penetrate through the porous structure 8 to contact with the root system of the plant, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

Wherein the culture soil is packaged by a sampling bag and then sterilized in a high-temperature sterilization pot.

Sterile water can be obtained by various sterilization methods, such as steam, UHT heat, ultraviolet sterilization, ozone, and physical filtration. Other components can be added into the sterile water according to the experimental requirements.

The amount of the sterile water is adjusted to enable the water storage area 5 of the integrated container to store a certain amount of sterile water without overflowing from the porous structure, the real-time water storage amount of the water storage area 5 can be directly observed, the sterile water in the water storage area 5 can maintain the soil humidity for a long time, and the soil environment required by plant growth is maintained.

The diameter of the plant cultivation area 7 is 60mm, the height of the plant cultivation area is 50mm, the interval between the microorganism cultivation areas 22 is 30mm, the height of the microorganism cultivation areas is 20mm, the interval between the plant cultivation area 77 and the microorganism cultivation areas 22 is 5mm, and the width of the connection area 6 is 5mm and the height of the connection area is 5 mm.

The porous structure 88 has 5 rows of small holes with a row spacing of 2mm, a hole diameter of 1mm, a hole spacing of 3mm, and a water storage area 5mm high of 20 mm.

The diameter of the bottom tank is 137mm, and the height of the bottom tank is 70 mm.

The thickness of the wall 4 of the plant cultivation area is 1 mm.

Example two:

the whole material of the device is plastic. For recyclable plastic materials, the present invention can be recycled by performing a sterilization treatment according to the first embodiment. The embodiment is characterized in that the device can be used for designing and producing the disposable experimental device due to simple design, and the disposable experimental device is formed by injection molding through designing a mold, so that the production process is simple and efficient, and the mass production can be realized. Carry out aseptic processing and packing to the product, need not to carry out secondary sterilization during the use, directly open the use in super clean bench, the random access helps improving work efficiency, avoids secondary pollution.

Example three:

this embodiment is different from the embodiment in a usage manner. In this example, the experiment was conducted in such a manner that the closed vessel was sterilized as a whole after the culture soil had been prefilled. The method comprises the following specific steps:

step 1: after the plant cultivation area 7 is filled with culture soil, a sealing cover or a film is added for sealing, the bottom tank opening 9 is hermetically connected with the connecting structure 6, the bottom tank is connected and combined with the integrated container, and a sealed inner environment is formed in an inner cavity 11 of the bottom tank;

step 2: putting the integrated container into a sterilization pot for integral sterilization, and forming an aseptic environment in the inner cavity 11 of the bottom tank after sterilization;

step 3: the integrated container is placed on a superclean bench upside down, the high-temperature sterilized solid culture medium is poured into the microorganism culture area 2, after the solid culture medium is cooled, the bottom tank is opened, the target strain is inoculated on the culture medium, the bottom tank is hermetically connected with the connecting structure 6 through the bottom tank opening 9, the bottom tank and the integrated container are connected and combined, and the integrated container is placed in the forward direction;

step 4: after the colony culture is completed, the sealing cover is opened or the covering film is covered, seeds are buried, and sterile water is added.

Step 5: after the bacterial colony is cultured, the volatile secondary metabolites of the microorganisms penetrate through the porous structure 8 to contact with the root system of the plant, and the growth parameters of the plant are measured by observing the growth vigor of the plant, so that different action effects of the volatile metabolites released by different microorganisms on different stages of the plant growth are obtained.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.