阅读说明：本技术 一种利用n-乙酰氨基葡萄糖母液制备涂抹剂的方法 (Method for preparing smearing preparation by using N-acetylglucosamine mother liquor ) 是由 卢健行 梁剑光 蒋萧 韩宁 张建华 巩凤英 于 2021-10-29 设计创作，主要内容包括：本发明公开了一种利用N-乙酰氨基葡萄糖母液制备涂抹剂的方法。涂抹剂的有效成分包括以下重量份的原料：N-乙酰氨基葡萄糖母液20～40份,薄荷醇2～4份,当归提取物5～10份,三七粉5～10份。N-乙酰氨基葡萄糖母液通过N-乙酰氨基葡萄糖发酵液调pH、灭菌,再依次进行陶瓷膜过滤、脱色、电渗析脱盐、浓缩,即得到N-乙酰氨基葡萄糖母液。本发明将N-乙酰氨基葡萄糖母液制成涂抹剂,同时辅以薄荷醇和当归提取物,即能促进N-乙酰氨基葡萄糖的吸收,同时对提高骨密度和关节疼痛的治疗起到协同作用,进一步扩宽了N-乙酰氨基葡萄糖的应用。(The invention discloses a method for preparing a smearing preparation by using N-acetylglucosamine mother liquor. The effective components of the smearing preparation comprise the following raw materials in parts by weight: 20-40 parts of N-acetylglucosamine mother liquor, 2-4 parts of menthol, 5-10 parts of angelica sinensis extract and 5-10 parts of pseudo-ginseng powder. Adjusting the pH value of the N-acetylglucosamine mother liquor by using an N-acetylglucosamine fermentation liquor, sterilizing, sequentially carrying out ceramic membrane filtration, decolorization, electrodialysis desalination and concentration to obtain the N-acetylglucosamine mother liquor. The N-acetylglucosamine mother liquor is prepared into the liniment, and menthol and the angelica sinensis extract are simultaneously used as auxiliary materials, so that the absorption of the N-acetylglucosamine can be promoted, the synergistic effect on the treatment of improving bone density and arthralgia is achieved, and the application of the N-acetylglucosamine is further widened.)

1. The pharmaceutical composition is characterized by comprising the following raw materials in parts by weight:

20-40 parts of N-acetylglucosamine mother liquor, 2-4 parts of menthol, 5-10 parts of angelica sinensis extract and 5-10 parts of pseudo-ginseng powder.

2. The pharmaceutical composition of claim 1, wherein the N-acetylglucosamine mother liquor is prepared by the following method:

(1) adjusting pH of the N-acetylglucosamine fermentation solution to 3.5-4.5, heating for sterilization, and denaturing, inactivating and precipitating the hybrid protein;

(2) and then sequentially carrying out ceramic membrane filtration, decolorization, electrodialysis desalination and concentration to obtain the N-acetylglucosamine mother liquor.

3. The pharmaceutical composition according to claim 2, wherein in step (2), the ceramic membrane has a pore size of 5 to 40 nm.

4. The pharmaceutical composition according to claim 2, wherein in the step (2), the decolorization is performed using activated carbon and diatomaceous earth; the mass ratio of the activated carbon to the diatomite is (0.5-1): 1, and the mass ratio of the filtrate to the activated carbon is 100: (0.5 to 1).

5. The pharmaceutical composition of claim 2, wherein in step (2), the electrodialysis desalination adopts a compact homogeneous ion exchange membrane, the volume ratio of the desalination chamber to the concentration chamber is controlled to be (3-5):1, the final conductivity of the desalted solution is controlled to be less than 300 μ s/cm, the working voltage is controlled to be 20-40V, and the flow rate is controlled to be 18-25L/h.

6. The pharmaceutical composition of claim 2, wherein in step (2), the concentration is: and (3) concentrating the solution subjected to electrodialysis desalination in vacuum at a vacuum degree of more than 0.9MPa and at a temperature of 45-60 ℃ until the solution is saturated.

7. The pharmaceutical composition of claim 1, wherein the angelica sinensis extract is prepared by the following method:

mixing the angelica and water for water extraction, and then performing rotary evaporation on the filtered liquid until the relative density is 1.2-1.3 to obtain the angelica extract.

8. Use of a pharmaceutical composition according to any one of claims 1 to 7 for the preparation of a spread.

9. A liniment characterized by comprising the pharmaceutical composition according to any one of claims 1 to 7 as an active ingredient.

10. Use of the spread according to claim 9 for relieving joint pain or increasing bone density.

Technical Field

The invention relates to the technical field of joint medicines, in particular to a method for preparing a smearing preparation by using N-acetylglucosamine mother liquor.

Background

Currently, N-acetylglucosamine (GlcNAc) is obtained by fermentation of genetically engineered bacteria, and the fermentation broth is subjected to filtration, impurity removal, separation, purification and refining processes to obtain a refined glucosamine hydrochloride or glucosamine sulfate powder. Because domestic manufacturers and downstream products mainly comprise glucosamine hydrochloride or glucosamine hydrochloride, N-acetylglucosamine is relatively less in the appearance of final products, and therefore, N-acetylglucosamine is not common in common consumer varieties. The process for preparing the glucosamine hydrochloride has longer steps, higher difficulty in quality control and large amount of waste water generated in the preparation process, and is not beneficial to environmental protection, energy conservation and emission reduction.

From the chemical composition and structure, N-acetylglucosamine is a glucose derivative and is usually present as a white powder. The solubility of N-acetylglucosamine is high, the solubility of water solvent species at 0 ℃ is 140g/L, and the N-acetylglucosamine is difficult to dissolve in organic solvents such as propanol, ethanol, acetone and the like. Good thermal stability, and melting point of about 200 ℃. N-acetylglucosamine plays an important role in physiological and metabolic synthesis in human body, animals, plants and microorganisms. Therefore, the N-acetylglucosamine can be widely used in the industries of food, medicine and cosmetics, the elderly population in China is aged, and the addition of the N-acetylglucosamine in diet and daily diet is beneficial to preventing osteoporosis, reducing the occurrence of diseases such as arthrosis and the like, and bringing benefits to the life health of people. However, most of the products currently on the market exist in the form of glucosamine hydrochloride or glucosamine sulfate products, and osteoarthritis is prevented and treated by oral administration of glucosamine hydrochloride or glucosamine sulfate. How to effectively utilize N-acetylglucosamine and in which form the N-acetylglucosamine is utilized by people are less related reports. If N-acetylglucosamine can be prepared into an ointment or a smearing preparation which is convenient to carry, the application of the N-acetylglucosamine is widened by directly using the N-acetylglucosamine in a mode of external smearing and absorption instead of oral administration.

Disclosure of Invention

In view of the prior art, the invention aims to provide a method for preparing a smearing preparation by using N-acetylglucosamine mother liquor. The N-acetylglucosamine mother liquor is prepared into the liniment, and menthol and the angelica sinensis extract are simultaneously used as auxiliary materials, so that the absorption of the N-acetylglucosamine can be promoted, the synergistic effect on the treatment of improving bone density and arthralgia is achieved, and the application of the N-acetylglucosamine is further widened.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides a pharmaceutical composition, which comprises the following raw materials in parts by weight: 40-50 parts of N-acetylglucosamine mother liquor, 2-4 parts of menthol, 5-10 parts of angelica sinensis extract and 5-10 parts of pseudo-ginseng powder.

Preferably, the N-acetylglucosamine mother liquor is prepared by the following method:

(1) adjusting pH of the N-acetylglucosamine fermentation solution to 3.5-4.5, heating for sterilization, and denaturing, inactivating and precipitating the hybrid protein;

(2) and then sequentially carrying out ceramic membrane filtration, decolorization, electrodialysis desalination and concentration to obtain the N-acetylglucosamine mother liquor.

Preferably, in the step (2), the pore diameter of the ceramic membrane is 5-40 nm.

Preferably, in the step (2), activated carbon and diatomite are used for decolorization; the mass ratio of the activated carbon to the diatomite is (0.5-1): 1, and the mass ratio of the filtrate to the activated carbon is 100: (0.5 to 1).

Preferably, in the step (2), the electrodialysis desalination adopts a compact homogeneous ion exchange membrane, the volume ratio of the desalination chamber to the concentration chamber is controlled to be (3-5):1, the final conductivity of the desalted solution is controlled to be less than 300 mu s/cm, the working voltage is controlled to be 20-40V, and the flow rate is controlled to be 18-25L/h.

Preferably, in step (2), the concentration is: and (3) concentrating the solution subjected to electrodialysis desalination in vacuum at a vacuum degree of more than 0.9MPa and at a temperature of 45-60 ℃ until the solution is saturated.

Preferably, the angelica sinensis extract is prepared by the following method:

mixing radix Angelicae sinensis and water according to a ratio of 1: 5, then carrying out water extraction, and carrying out rotary evaporation on the filtered liquid until the relative density is 1.2-1.3 to obtain the angelica sinensis extract.

In a second aspect of the invention, the application of the pharmaceutical composition in preparing a smearing preparation is provided.

In a third aspect of the present invention, there is provided a coating agent comprising the pharmaceutical composition as an active ingredient.

The pharmaceutical composition can be prepared into a smearing preparation and can be prepared into a plaster by being matched with other auxiliary materials. After smearing, gauze, non-woven fabric, plastic tape and the like can be applied.

In a fourth aspect of the invention, the application of the smearing preparation in relieving joint pain or increasing bone density is provided.

The invention has the beneficial effects that:

(1) the invention solves the problem that only glucosamine hydrochloride can be used for improving bone density or relieving joint pain in the current market. The preparation process of the glucosamine hydrochloride has longer steps, higher quality control difficulty and large amount of waste water generated in the preparation process, is not beneficial to environmental protection, energy conservation and emission reduction, and the invention does not need the steps, only needs to concentrate the N-acetylglucosamine fermentation liquor, reduces the process steps, simultaneously enlarges the application field of the N-acetylglucosamine, greatly increases the requirement of common people on supplementing the glucosamine and has huge economic value.

(2) According to the invention, researches show that the N-acetylglucosamine can be used together with menthol, angelica extract and pseudo-ginseng powder to improve the absorption effect of the N-acetylglucosamine, and can play a synergistic role in improving bone density and relieving arthralgia.

(3) The smearing preparation prepared by the invention is carried and used preferentially, not only has the health-care function of glucosamine, but also has a certain treatment function and combines prevention and treatment. It is suitable for the middle aged and the elderly people and people with frequent exercise for long term use, and has no side effect.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

As described in the background section, N-acetylglucosamine obtained by fermentation is difficult to purify and crystallize, so that glucosamine can be used only after being changed into glucosamine hydrochloride at present, and N-acetylglucosamine is not directly utilized.

Based on the technical scheme, the invention aims to provide a method for preparing a smearing preparation by using N-acetylglucosamine mother liquor. The invention firstly purifies and concentrates N-acetylglucosamine mother liquor to obtain N-acetylglucosamine saturated liquid (mother liquor), and then compounds the N-acetylglucosamine saturated liquid (mother liquor) with menthol, angelica extract and pseudo-ginseng powder. While menthol can promote the transdermal absorption of many drugs, when the N-acetylglucosamine mother liquor is mixed with menthol in a 10: 1, the N-acetyl of the human body has the best effect of absorbing the glucosamine, and the time lag for the N-acetyl glucosamine to reach the maximum permeation quantity is the shortest. The inventor finds that the menthol, the angelica sinensis extract and the pseudo-ginseng powder can more effectively promote the absorption rate and the absorption effect of a human body on the N-acetylglucosamine through experiments. And because the angelica has the functions of enriching and activating blood, the pseudo-ginseng powder has the effects of activating blood, dissolving stasis and relieving pain, and the function of relieving arthralgia is further improved through synergistic action. The smearing preparation prepared by the invention can prevent and treat joint diseases, relieve pain and swelling, repair joints, improve bone density and improve osteoporosis, and has outstanding effects.

In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.

The test materials used in the examples of the present invention are all conventional in the art and commercially available.

Description of the drawings: examples the N-acetylglucosamine fermentation broth used was from Hiden Bio-technology Ltd, and the N-acetylglucosamine fermentation broth was a fermentation broth for the preparation of glucosamine hydrochloride or glucosamine sulfate.

The Notoginseng radix powder can be obtained by purchasing commercially available product or pulverizing Notoginseng radix rhizome (main root) into powder.

Example 1: preparation of N-acetylglucosamine mother liquor

(1) The pH of the N-acetylglucosamine fermentation broth is adjusted to 4.0 by concentrated sulfuric acid.

(2) The prepared N-acetylglucosamine fermentation liquor with the pH value of 4.0 is quickly heated to 85 ℃ by high-temperature steam, and thalli are inactivated for 30min, so that the thalli are ensured to be inactivated, and the denaturation and precipitation of hybrid proteins are facilitated.

(3) Filtering with ceramic membrane (pore size: 40nm), and collecting filtrate.

(4) Adding activated carbon and diatomite into the collected filtrate for decolorization, and collecting decolorized solution. The mass ratio of the activated carbon to the diatomite is 1: 1, the mass ratio of the filtrate to the activated carbon is 100: 1.

(5) the electrodialysis desalination adopts a compact homogeneous phase ion exchange membrane, the volume ratio of a desalination chamber to a concentration chamber is controlled to be 3:1, the final conductivity of the desalted solution is controlled to be below 300 mu s/cm, the working voltage is controlled to be 20V, and the flow rate is controlled to be 18L/h.

(6) And (3) carrying out vacuum concentration on the solution subjected to electrodialysis desalination until the solution is saturated under the condition that the vacuum degree is more than 0.9MPa and the temperature is controlled at 45 ℃, thus obtaining the N-acetylglucosamine mother liquor.

Example 2: angelica sinensis extract

Pulverizing radix Angelicae sinensis, mixing with water according to a ratio of 1: 5, extracting with water at 100 ℃ for 30min, adding 3 times by mass of warm water at 60 ℃ into the filtered filter residue, extracting with water at 100 ℃ for 15min, and mixing the filtered filtrate with the filtrate obtained by the first water extraction. The filtrate is rotary evaporated at 80 ℃ under the vacuum degree of 0.08MPa until the relative density reaches 1.26. The obtained thick paste is the angelica extract.

Example 3

30g of the N-acetylglucosamine mother liquor prepared in example 1, 3g of menthol, 7.5g of the Angelica sinensis extract prepared in example 2, and 7.5g of Panax notoginseng powder were mixed uniformly to obtain a liniment.

Example 4

20g of the N-acetylglucosamine mother liquor prepared in example 1, 2g of menthol, 10g of the Angelica sinensis extract prepared in example 2, and 5g of Panax notoginseng powder were mixed uniformly to obtain a liniment.

Example 5

40g of the N-acetylglucosamine mother liquor prepared in example 1, 4g of menthol, 5g of the Angelica sinensis extract prepared in example 2, and 10g of Panax notoginseng powder were mixed uniformly to obtain a liniment.

Comparative example 1

30g of the N-acetylglucosamine mother liquor prepared in example 1 and 3g of menthol were mixed uniformly to obtain a liniment.

Comparative example 2

30g of the N-acetylglucosamine mother liquor prepared in example 1 and 7.5g of the Angelica sinensis extract prepared in example 2 were mixed uniformly to obtain a liniment.

Comparative example 3

30g of the N-acetylglucosamine mother liquor prepared in example 1 and 7.5g of pseudo-ginseng powder were mixed uniformly to obtain a liniment.

Test example 1

120 cases of knee osteoarthritis patients are selected, and the male and female proportion is 1: 1, the coating agents prepared by the methods of examples 3-5 and comparative examples 1-3 are randomly divided into 6 groups, and the application method is that the coating agents are applied 3 times a day, and 5g of the coating agents are applied each time (after the coating agents are uniformly applied, non-woven fabrics can be applied). After 2 months of continuous use, the improvement of symptoms and the structural and functional changes of articular cartilage of each group were evaluated by using the WOMAC osteoarthritis index score and the VAS pain score scale, and the average values were calculated, and the results are shown in tables 1 and 2.

WOMAC osteoarthritis index score: including pain, stiffness, joint function, for a total of 24 items. The higher WOMAC index indicates more severe osteoarthritis, and the severity of osteoarthritis is evaluated according to the following criteria, based on the total score: the mild degree is less than or equal to 80, the moderate degree is 80-120, and the severe degree is more than or equal to 120.

VAS pain score scale: a10 cm ruler is adopted, 0 and 10 are marked at two ends, and the corresponding positions of self pain or limited function degree are marked on the ruler by a patient. The average value of mild pain is 2.57 +/-1.04, the average value of moderate pain is 5.18 +/-1.41, and the average value of severe pain is 8.41 +/-1.35.

Table 1 WOMAC test results

Item	Before use	After 1 month of use	After 2 months of use
				Example 3	70.2±8.2	59.8±6.3	45.2±7.4
Example 4	73.4±5.6	63.6±7.4	50.1±6.8
				Example 5	71.7±6.8	61.2±4.1	47.0±9.0
Comparative example 1	69.8±6.5	64.4±4.9	56.7±5.2
				Comparative example 2	74.9±7.1	71.3±8.0	67.6±5.6
Comparative example 3	71.9±7.8	70.8±8.0	69.1±8.3

TABLE 2 VAS test results

Item	Before use	After 1 month of use	After 2 months of use
				Example 3	7.2±1.3	6.2±1.4	4.9±
Example 4	7.2±0.9	6.4±0.8	5.2±
				Example 5	6.9±1.2	6.1±0.7	5.0±
Comparative example 1	7.2±0.8	6.6±1.5	6.0±
				Comparative example 2	6.9±1.3	6.7±1.3	6.3±
Comparative example 3	6.8±1.5	6.7±1.4	6.6±

As can be seen from tables 1 and 2, the application prepared by the method of example 3 is the most effective, and has the effects of relieving joint pain and promoting improvement of joint movement. According to data comparison, the angelica sinensis extract can promote the absorption of the N-acetylglucosamine besides the menthol, but the menthol, the angelica sinensis extract and the pseudo-ginseng powder can synergistically promote the absorption of the N-acetylglucosamine and have the optimal effect of relieving the arthralgia.

Test example 2

300 basketball fans of 18-35 years old are selected as volunteers, and the problems of joint and muscle pain occur during sports. The volunteers were randomly divided into 6 groups of 50 persons, each group using the smearing agents prepared in examples 3-5 and comparative examples 1-3, 1 time per day, 5g each time (after smearing uniformly, non-woven fabric can be applied), and the application was continued for 45 days. The time to onset was scored and the results averaged (rounded to an integer) and reported in table 3 and table 4.

TABLE 3

TABLE 4

As shown in Table 4, the smearing preparation prepared by the method of example 3 can rapidly relieve joint and muscle pain, and compared with comparative examples 1-3, the smearing preparation has the advantages of short time and quick response in joint and muscle pain, joint tissue relaxation and normal function recovery.

Compared with the comparative examples 1-3, the smearing preparation prepared in the comparative example 3 is quick in effect in a short time, and the effect is quick in a short time for relieving joint and muscle pain because the pseudo-ginseng powder is contained in the comparative example 3 and can promote blood circulation, remove blood stasis and relieve pain. However, in long-term use, N-acetylglucosamine is mainly used for relieving joint tissues and restoring normal functions, so that the N-acetylglucosamine has high permeation rate and good effect in long-term use. As can be seen from comparative example 2, the Angelica sinensis extract also has a certain penetration-promoting effect on N-acetylglucosamine, but the effect is still inferior to that of menthol as compared with comparative example 1. However, the menthol, the angelica sinensis extract and the pseudo-ginseng powder can synergistically promote the permeation of the N-acetylglucosamine, and can effectively relieve joint tissues and restore normal functions after long-term use, and the effect taking speed is high.

Test example 3

A batch of 120 patients with primary osteoporosis was randomly drawn, with a male-female ratio of 1: 1, age 60-80 years old. All patients do not suffer from rheumatism, endocrine, immunity and other diseases affecting bone metabolism. The groups were randomly divided into 6 groups. The smearing agents prepared in the methods of examples 3-5 and comparative examples 1-3 are respectively used for each group, and the application method is that the smearing agents are smeared on legs 3 times a day, 5g of the smearing agents are smeared each time (after the smearing agents are evenly smeared, plastic bands can be tied on the smeared legs). The application period is 2 months. BMD values of the front and rear legs of 6 groups of patients using the smearing agent were measured by an ultrasonic bone densitometer, and averaged, and the results are shown in Table 5.

TABLE 5

As can be seen from table 5, the application prepared by the method of example 3 is effective in improving osteoporosis and increasing bone density. Compared with comparative examples 1-3, after the menthol, the angelica sinensis extract and the pseudo-ginseng powder are compounded, the absorption capacity of the N-acetylglucosamine can be effectively improved, and the bone density is improved through a synergistic effect.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.