阅读说明：本技术 修饰的末端脱氧核苷酸转移酶(TdT) (Modified terminal deoxynucleotidyl transferase (TdT) ) 是由 迈克尔·春·浩·陈 戈登·罗斯·米钦罗伊 伊恩·哈斯顿·库克 陈思红 于 2020-02-04 设计创作，主要内容包括：本发明涉及工程化末端脱氧核苷酸转移酶(TdT)或任何物种的Polμ、Polβ、Polλ和Polθ的同源氨基酸序列或任何物种的X家族聚合酶的同源氨基酸序列及其在核酸合成的方法中的用途,涉及合成核酸的方法,以及涉及包含所述酶的试剂盒在核酸合成的方法中的用途。本发明还涉及新的末端脱氧核苷酸转移酶和3’-封闭的核苷三磷酸在非模板依赖性核酸合成的方法中的用途。(The present invention relates to an engineered terminal deoxynucleotidyl transferase (TdT) enzyme or homologous amino acid sequence of Pol μ, Pol β, Pol λ and Pol θ of any species or homologous amino acid sequence of X family polymerase of any species and use thereof in a method of nucleic acid synthesis, to a method of synthesizing nucleic acids, and to the use of a kit comprising said enzyme in a method of nucleic acid synthesis. The invention also relates to the use of novel terminal deoxynucleotidyl transferases and 3' -blocked nucleoside triphosphates in methods for template-independent nucleic acid synthesis.)